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Thermo Fisher mfe i
Mfe I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mfe i/product/Thermo Fisher
Average 89 stars, based on 3 article reviews
Price from $9.99 to $1999.99
mfe i - by Bioz Stars, 2020-09
89/100 stars

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Clone Assay:

Article Title: A Two-hybrid Screen Identifies Cathepsins B and L as Uncoating Factors for Adeno-associated Virus 2 and 8
Article Snippet: .. The cathepsin B and L PCR products were digested with Eco RI and Xho I, or Mfe I and Xho I, respectively, and then cloned into the pcDNA3 (Invitrogen, Carlsbad, CA) vector digested with Eco RI and Xho I. Yeast two-hybrid screen. .. The initial two-hybrid screen was conducted using the AAV8 bait plasmid, the mouse liver Matchmaker cDNA library (BD Clontech), and the yeast strain AH109 (MATa, trp1-901, leu2-3, leu2-112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1 UAS -GAL1 TATA -HIS3, GAL2 UAS -GAL2 TATA -ADE2, URA3::MEL1 UAS -MEL1 TATA -lacZ ) (BD Clontech).

Article Title: The Alternative Route to Heme in the Methanogenic Archaeon Methanosarcina barkeri
Article Snippet: .. For the construction of plasmid pETDuetahbD the codon-optimized gene Mbar_A1458 (encoding AhbD) was PCR amplified using the primers NirJ1_Duet_Mfe I_fw ( C AAT TG G ATG ATT GCC ATG ACC AAT G) and NirJ1_pGEX_Xho I_bw (G CTC GAG TTA TTT TTT ACC CGG AC) containing Mfe I and Xho I restriction sites (underlined) and first cloned into the pJET1.2/blunt cloning vector (Thermo Scientific) according to the manufacturer's instructions. .. Mbar_A1458 was then cut from this plasmid with Mfe I and Xho I endonucleases and the resulting DNA fragment was ligated into the correspondingly digested vector pETDuet-1 (Novagen) generating pETDuetahbD .

Article Title: Inactivation of hnRNP K by Expanded Intronic AUUCU Repeat Induces Apoptosis Via Translocation of PKC? to Mitochondria in Spinocerebellar Ataxia 10
Article Snippet: .. For stable over-expression of hnRNP K in Sy5y cells, pcDNA-hnRNP K was digested with Mfe I and the linear plasmid DNA was tranefected into Sy5y cells using Lipofectamin 2000 reagent (Invitrogen), and the stable clones were selected with hygromycin (300 mg/ml). .. Total protein was isolated from the stably transfected cells and the hnRNP K expression level was analyzed by Western blotting with the anti-hnRNP K antibody.

Amplification:

Article Title: Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis
Article Snippet: .. Restriction Fragment Length Polymorphism (RFLP) Analysis 2200 bp bands amplified from gDNA of five +B and five 0B mice were removed from the agarose gels, purified with QIAquick Gel Extraction Kit (Qiagen) and then digested with twelve restriction endonucleases (RE): Apa I, Hind III, Bsp 120I, Mfe I, Hpa I, Msp I, Mbo I, Alu I, Mse I, Ecl 136II, Hae III and Sac I (FastDigest, Thermo Scientific, Waltham, MA USA) according to the manufacturer’s instructions. .. Digested DNA fragments were separated by agarose gel electrophoresis, visualized and photo-documented as above.

Article Title: The Alternative Route to Heme in the Methanogenic Archaeon Methanosarcina barkeri
Article Snippet: .. For the construction of plasmid pETDuetahbD the codon-optimized gene Mbar_A1458 (encoding AhbD) was PCR amplified using the primers NirJ1_Duet_Mfe I_fw ( C AAT TG G ATG ATT GCC ATG ACC AAT G) and NirJ1_pGEX_Xho I_bw (G CTC GAG TTA TTT TTT ACC CGG AC) containing Mfe I and Xho I restriction sites (underlined) and first cloned into the pJET1.2/blunt cloning vector (Thermo Scientific) according to the manufacturer's instructions. .. Mbar_A1458 was then cut from this plasmid with Mfe I and Xho I endonucleases and the resulting DNA fragment was ligated into the correspondingly digested vector pETDuet-1 (Novagen) generating pETDuetahbD .

Two Hybrid Screening:

Article Title: A Two-hybrid Screen Identifies Cathepsins B and L as Uncoating Factors for Adeno-associated Virus 2 and 8
Article Snippet: .. The cathepsin B and L PCR products were digested with Eco RI and Xho I, or Mfe I and Xho I, respectively, and then cloned into the pcDNA3 (Invitrogen, Carlsbad, CA) vector digested with Eco RI and Xho I. Yeast two-hybrid screen. .. The initial two-hybrid screen was conducted using the AAV8 bait plasmid, the mouse liver Matchmaker cDNA library (BD Clontech), and the yeast strain AH109 (MATa, trp1-901, leu2-3, leu2-112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1 UAS -GAL1 TATA -HIS3, GAL2 UAS -GAL2 TATA -ADE2, URA3::MEL1 UAS -MEL1 TATA -lacZ ) (BD Clontech).

Purification:

Article Title: Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis
Article Snippet: .. Restriction Fragment Length Polymorphism (RFLP) Analysis 2200 bp bands amplified from gDNA of five +B and five 0B mice were removed from the agarose gels, purified with QIAquick Gel Extraction Kit (Qiagen) and then digested with twelve restriction endonucleases (RE): Apa I, Hind III, Bsp 120I, Mfe I, Hpa I, Msp I, Mbo I, Alu I, Mse I, Ecl 136II, Hae III and Sac I (FastDigest, Thermo Scientific, Waltham, MA USA) according to the manufacturer’s instructions. .. Digested DNA fragments were separated by agarose gel electrophoresis, visualized and photo-documented as above.

Polymerase Chain Reaction:

Article Title: A Two-hybrid Screen Identifies Cathepsins B and L as Uncoating Factors for Adeno-associated Virus 2 and 8
Article Snippet: .. The cathepsin B and L PCR products were digested with Eco RI and Xho I, or Mfe I and Xho I, respectively, and then cloned into the pcDNA3 (Invitrogen, Carlsbad, CA) vector digested with Eco RI and Xho I. Yeast two-hybrid screen. .. The initial two-hybrid screen was conducted using the AAV8 bait plasmid, the mouse liver Matchmaker cDNA library (BD Clontech), and the yeast strain AH109 (MATa, trp1-901, leu2-3, leu2-112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1 UAS -GAL1 TATA -HIS3, GAL2 UAS -GAL2 TATA -ADE2, URA3::MEL1 UAS -MEL1 TATA -lacZ ) (BD Clontech).

Article Title: The Alternative Route to Heme in the Methanogenic Archaeon Methanosarcina barkeri
Article Snippet: .. For the construction of plasmid pETDuetahbD the codon-optimized gene Mbar_A1458 (encoding AhbD) was PCR amplified using the primers NirJ1_Duet_Mfe I_fw ( C AAT TG G ATG ATT GCC ATG ACC AAT G) and NirJ1_pGEX_Xho I_bw (G CTC GAG TTA TTT TTT ACC CGG AC) containing Mfe I and Xho I restriction sites (underlined) and first cloned into the pJET1.2/blunt cloning vector (Thermo Scientific) according to the manufacturer's instructions. .. Mbar_A1458 was then cut from this plasmid with Mfe I and Xho I endonucleases and the resulting DNA fragment was ligated into the correspondingly digested vector pETDuet-1 (Novagen) generating pETDuetahbD .

Gel Extraction:

Article Title: Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis
Article Snippet: .. Restriction Fragment Length Polymorphism (RFLP) Analysis 2200 bp bands amplified from gDNA of five +B and five 0B mice were removed from the agarose gels, purified with QIAquick Gel Extraction Kit (Qiagen) and then digested with twelve restriction endonucleases (RE): Apa I, Hind III, Bsp 120I, Mfe I, Hpa I, Msp I, Mbo I, Alu I, Mse I, Ecl 136II, Hae III and Sac I (FastDigest, Thermo Scientific, Waltham, MA USA) according to the manufacturer’s instructions. .. Digested DNA fragments were separated by agarose gel electrophoresis, visualized and photo-documented as above.

Antiviral Assay:

Article Title: The role of the tyrosine kinase Wzc (Sll0923) and the phosphatase Wzb (Slr0328) in the production of extracellular polymeric substances (EPS) by Synechocystis PCC 6803, et al. The role of the tyrosine kinase Wzc (Sll0923) and the phosphatase Wzb (Slr0328) in the production of extracellular polymeric substances (EPS) by Synechocystis PCC 6803
Article Snippet: .. 2.5 Southern blots Southern blots were performed using genomic DNA of the wild type and mutants digested with Bam HI (∆wzy and ∆kpsM ∆wzy ), Eco RI (∆kpsM and ∆kpsM ∆wzy ), Ava II (∆wzx ), Nco I (∆wzc, wzc Trunc and ∆wzc ∆wzb ), and/or Mfe I (∆wzb and ∆wzc ∆wzb ) (Thermo Scientific). .. The DNA fragments were separated by electrophoresis on a 1% agarose gel and blotted onto Amersham HybondTM ‐N membrane (GE Healthcare).

Mouse Assay:

Article Title: Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis
Article Snippet: .. Restriction Fragment Length Polymorphism (RFLP) Analysis 2200 bp bands amplified from gDNA of five +B and five 0B mice were removed from the agarose gels, purified with QIAquick Gel Extraction Kit (Qiagen) and then digested with twelve restriction endonucleases (RE): Apa I, Hind III, Bsp 120I, Mfe I, Hpa I, Msp I, Mbo I, Alu I, Mse I, Ecl 136II, Hae III and Sac I (FastDigest, Thermo Scientific, Waltham, MA USA) according to the manufacturer’s instructions. .. Digested DNA fragments were separated by agarose gel electrophoresis, visualized and photo-documented as above.

Over Expression:

Article Title: Inactivation of hnRNP K by Expanded Intronic AUUCU Repeat Induces Apoptosis Via Translocation of PKC? to Mitochondria in Spinocerebellar Ataxia 10
Article Snippet: .. For stable over-expression of hnRNP K in Sy5y cells, pcDNA-hnRNP K was digested with Mfe I and the linear plasmid DNA was tranefected into Sy5y cells using Lipofectamin 2000 reagent (Invitrogen), and the stable clones were selected with hygromycin (300 mg/ml). .. Total protein was isolated from the stably transfected cells and the hnRNP K expression level was analyzed by Western blotting with the anti-hnRNP K antibody.

Plasmid Preparation:

Article Title: A Two-hybrid Screen Identifies Cathepsins B and L as Uncoating Factors for Adeno-associated Virus 2 and 8
Article Snippet: .. The cathepsin B and L PCR products were digested with Eco RI and Xho I, or Mfe I and Xho I, respectively, and then cloned into the pcDNA3 (Invitrogen, Carlsbad, CA) vector digested with Eco RI and Xho I. Yeast two-hybrid screen. .. The initial two-hybrid screen was conducted using the AAV8 bait plasmid, the mouse liver Matchmaker cDNA library (BD Clontech), and the yeast strain AH109 (MATa, trp1-901, leu2-3, leu2-112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1 UAS -GAL1 TATA -HIS3, GAL2 UAS -GAL2 TATA -ADE2, URA3::MEL1 UAS -MEL1 TATA -lacZ ) (BD Clontech).

Article Title: The Alternative Route to Heme in the Methanogenic Archaeon Methanosarcina barkeri
Article Snippet: .. For the construction of plasmid pETDuetahbD the codon-optimized gene Mbar_A1458 (encoding AhbD) was PCR amplified using the primers NirJ1_Duet_Mfe I_fw ( C AAT TG G ATG ATT GCC ATG ACC AAT G) and NirJ1_pGEX_Xho I_bw (G CTC GAG TTA TTT TTT ACC CGG AC) containing Mfe I and Xho I restriction sites (underlined) and first cloned into the pJET1.2/blunt cloning vector (Thermo Scientific) according to the manufacturer's instructions. .. Mbar_A1458 was then cut from this plasmid with Mfe I and Xho I endonucleases and the resulting DNA fragment was ligated into the correspondingly digested vector pETDuet-1 (Novagen) generating pETDuetahbD .

Article Title: Inactivation of hnRNP K by Expanded Intronic AUUCU Repeat Induces Apoptosis Via Translocation of PKC? to Mitochondria in Spinocerebellar Ataxia 10
Article Snippet: .. For stable over-expression of hnRNP K in Sy5y cells, pcDNA-hnRNP K was digested with Mfe I and the linear plasmid DNA was tranefected into Sy5y cells using Lipofectamin 2000 reagent (Invitrogen), and the stable clones were selected with hygromycin (300 mg/ml). .. Total protein was isolated from the stably transfected cells and the hnRNP K expression level was analyzed by Western blotting with the anti-hnRNP K antibody.

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    Thermo Fisher primers nirj1 duet mfe i fw
    Primers Nirj1 Duet Mfe I Fw, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primers nirj1 duet mfe i fw/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primers nirj1 duet mfe i fw - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    89
    Thermo Fisher mfe i
    Mfe I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mfe i/product/Thermo Fisher
    Average 89 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mfe i - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

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