hia5 mtase  (New England Biolabs)


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    New England Biolabs hia5 mtase
    Hia5 Mtase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mrna cap 2 o methyltransferase  (New England Biolabs)


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    New England Biolabs mrna cap 2 o methyltransferase
    Mrna Cap 2 O Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cpg methyltransferase  (New England Biolabs)


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    New England Biolabs cpg methyltransferase
    Cpg Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cpg methyltransferase  (New England Biolabs)


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    New England Biolabs cpg methyltransferase
    Cpg Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cpg methyltransferase  (New England Biolabs)


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    New England Biolabs cpg methyltransferase
    Cpg Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gpc methyltransferase  (New England Biolabs)


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    New England Biolabs gpc methyltransferase
    Gpc Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cpg methyltransferase  (New England Biolabs)


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    New England Biolabs cpg methyltransferase
    (A) Illustration of heat-induced mutagenesis on 5-meC-containing DNA. (B-E) Spectra of G>A mutations on C residues of templates with different sequence contexts. The templates were modified with 5-meC and treated with EcUdg as indicated. The control DNA without 5-meC modification was prepared by the same exact procedure, except that CpG <t>methyltransferase</t> was omitted. Mutations on NpCpG template contexts are indicated by triangles. (F) Effects of the 5-meC modification on G>A mutation frequencies without Udg treatment were compared within each trinucleotide context. ***p<0.0001 in a 2-way ANOVA with Tukey’s multiple comparisons (n values are shown under the graph). All other pairs are scored “ns”. (G) Illustration to explain how in vitro mutation frequency data was organized in comparable format of cancer mutational signature. In this example, strand extension occurs from left to right on the damaged template. During the DNA synthesis, two misincorporations (G>A in blue and C>T in green) occur on the extending strand. These changes are complementary to each other including the sequence context, making them indistinguishable by genome sequencing. Since our in vitro approach separately quantifies such complementary mutations on the extending strand, we show them at the same position on the graph using a blue bar (purine substitution) and a green bar (pyrimidine substitution). (H) Spectrum of mutations that were produced by yPol δ on the ssDNA containing 5-meC, which was treated by heat and then by EcUdg. (I) Cancer mutational signature 1 (Alexandrov et. al, 2020) after normalization of trinucleotides appearance in human genome.
    Cpg Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Biochemical reconstitution of a major age-related cancer mutational signature by heat-induced spontaneous deamination of 5-methylcytosine residues, repair of uracil residues, and DNA replication"

    Article Title: Biochemical reconstitution of a major age-related cancer mutational signature by heat-induced spontaneous deamination of 5-methylcytosine residues, repair of uracil residues, and DNA replication

    Journal: bioRxiv

    doi: 10.1101/2024.05.22.595323

    (A) Illustration of heat-induced mutagenesis on 5-meC-containing DNA. (B-E) Spectra of G>A mutations on C residues of templates with different sequence contexts. The templates were modified with 5-meC and treated with EcUdg as indicated. The control DNA without 5-meC modification was prepared by the same exact procedure, except that CpG methyltransferase was omitted. Mutations on NpCpG template contexts are indicated by triangles. (F) Effects of the 5-meC modification on G>A mutation frequencies without Udg treatment were compared within each trinucleotide context. ***p<0.0001 in a 2-way ANOVA with Tukey’s multiple comparisons (n values are shown under the graph). All other pairs are scored “ns”. (G) Illustration to explain how in vitro mutation frequency data was organized in comparable format of cancer mutational signature. In this example, strand extension occurs from left to right on the damaged template. During the DNA synthesis, two misincorporations (G>A in blue and C>T in green) occur on the extending strand. These changes are complementary to each other including the sequence context, making them indistinguishable by genome sequencing. Since our in vitro approach separately quantifies such complementary mutations on the extending strand, we show them at the same position on the graph using a blue bar (purine substitution) and a green bar (pyrimidine substitution). (H) Spectrum of mutations that were produced by yPol δ on the ssDNA containing 5-meC, which was treated by heat and then by EcUdg. (I) Cancer mutational signature 1 (Alexandrov et. al, 2020) after normalization of trinucleotides appearance in human genome.
    Figure Legend Snippet: (A) Illustration of heat-induced mutagenesis on 5-meC-containing DNA. (B-E) Spectra of G>A mutations on C residues of templates with different sequence contexts. The templates were modified with 5-meC and treated with EcUdg as indicated. The control DNA without 5-meC modification was prepared by the same exact procedure, except that CpG methyltransferase was omitted. Mutations on NpCpG template contexts are indicated by triangles. (F) Effects of the 5-meC modification on G>A mutation frequencies without Udg treatment were compared within each trinucleotide context. ***p<0.0001 in a 2-way ANOVA with Tukey’s multiple comparisons (n values are shown under the graph). All other pairs are scored “ns”. (G) Illustration to explain how in vitro mutation frequency data was organized in comparable format of cancer mutational signature. In this example, strand extension occurs from left to right on the damaged template. During the DNA synthesis, two misincorporations (G>A in blue and C>T in green) occur on the extending strand. These changes are complementary to each other including the sequence context, making them indistinguishable by genome sequencing. Since our in vitro approach separately quantifies such complementary mutations on the extending strand, we show them at the same position on the graph using a blue bar (purine substitution) and a green bar (pyrimidine substitution). (H) Spectrum of mutations that were produced by yPol δ on the ssDNA containing 5-meC, which was treated by heat and then by EcUdg. (I) Cancer mutational signature 1 (Alexandrov et. al, 2020) after normalization of trinucleotides appearance in human genome.

    Techniques Used: Mutagenesis, Sequencing, Modification, In Vitro, DNA Synthesis, Produced

    m sssi cpg methyltransferase  (New England Biolabs)


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    M Sssi Cpg Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mrna cap 2 o methyltransferase  (New England Biolabs)


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    New England Biolabs mrna cap 2 o methyltransferase
    Mrna Cap 2 O Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    o methyltransferase  (New England Biolabs)


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    New England Biolabs o methyltransferase
    O Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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