Journal: Applied microbiology and biotechnology
Article Title: Methanogenic partner influences cell aggregation and signalling of Syntrophobacterium fumaroxidans.
doi: 10.1007/s00253-023-12955-w
Figure Lengend Snippet: Fig. 3 A Scanning electron microscopy imaging of S. fumaroxidans (yellow arrow) aggregates with M. formicicum (1, sampled at Cycle 30) or M. hungatei (2, sampled at Cycle 34) (green arrow). B Overlayed epifluorescent imaging of the syntrophic aggregates with M. formicicum (1) or M. hungatei (2). Green florescence comes from the Syto 16 staining, while blue fluorescence comes from the autofluorescence of cofactor F420 (see “Materials and meth- ods” for details). Images A1.1, A2.1-2 have a scale bar of 5 μm; A1.3 has a 3 μm scale bar; A2.3 and B1-2 have a 10-μm scale bar
Article Snippet: Cultivation of microorganisms Pure cultures of Methanospirillum hungatei strain JF1 (DSM 864T), Methanobacterium formicicum strain MF (DSM 1535) and Syntrophobacterium fumaroxidans (formerly Syntrophobacter fumaroxidans strain MPOB, DSM 10017T) were obtained from the German Collection of Microorganisms and Cell Culture (DSMZ, Braunschweig, Germany).
Techniques: Electron Microscopy, Imaging, Staining, Fluorescence