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A) LSEC cell culture activation with differentiated metastatic colorectal tumor cells and colorectal CSC conditioned media. B) Immunofluorescent staining of PCSK9 (red), tubulin (green) and DAPI (blue) in human LSEC cell cultures in basal conditions and activation with differentiated metastatic colorectal tumor cells <t>SW620</t> and colorectal CSC-like from SW620 conditioned media. C) DAPI (blue), CD31(green) and PCSK9 (red) staining on liver tissue samples from colorectal cancer patients with liver metastases. The areas highlighted in the images in the upper row are shown enlarged in the lower row.
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A) LSEC cell culture activation with differentiated metastatic colorectal tumor cells and colorectal CSC conditioned media. B) Immunofluorescent staining of PCSK9 (red), tubulin (green) and DAPI (blue) in human LSEC cell cultures in basal conditions and activation with differentiated metastatic colorectal tumor cells <t>SW620</t> and colorectal CSC-like from SW620 conditioned media. C) DAPI (blue), CD31(green) and PCSK9 (red) staining on liver tissue samples from colorectal cancer patients with liver metastases. The areas highlighted in the images in the upper row are shown enlarged in the lower row.
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A) LSEC cell culture activation with differentiated metastatic colorectal tumor cells and colorectal CSC conditioned media. B) Immunofluorescent staining of PCSK9 (red), tubulin (green) and DAPI (blue) in human LSEC cell cultures in basal conditions and activation with differentiated metastatic colorectal tumor cells SW620 and colorectal CSC-like from SW620 conditioned media. C) DAPI (blue), CD31(green) and PCSK9 (red) staining on liver tissue samples from colorectal cancer patients with liver metastases. The areas highlighted in the images in the upper row are shown enlarged in the lower row.

Journal: bioRxiv

Article Title: PCSK9 Exhibits Novel Nuclear Localization in LSEC and Its Targeting with Bioinspired Nanoparticles Reduces Colorectal Liver Metastasis

doi: 10.64898/2026.05.26.727886

Figure Lengend Snippet: A) LSEC cell culture activation with differentiated metastatic colorectal tumor cells and colorectal CSC conditioned media. B) Immunofluorescent staining of PCSK9 (red), tubulin (green) and DAPI (blue) in human LSEC cell cultures in basal conditions and activation with differentiated metastatic colorectal tumor cells SW620 and colorectal CSC-like from SW620 conditioned media. C) DAPI (blue), CD31(green) and PCSK9 (red) staining on liver tissue samples from colorectal cancer patients with liver metastases. The areas highlighted in the images in the upper row are shown enlarged in the lower row.

Article Snippet: The human metastatic colorectal adenocarcinoma cell line SW620 and the murine metastatic colorectal adenocarcinoma cell line CT26 were acquired from the American Type Culture Collection (ATCC).

Techniques: Cell Culture, Activation Assay, Staining