mem neaa (Thermo Fisher)


Name:
MEM NEAA no glutamine
Description:
Minimum Essential Medium MEM is one of the most commonly used of all cell culture media MEM can be used with a variety of suspension and adherent mammalian cells including HeLa BHK 21 293 HEP 2 HT 1080 MCF 7 fibroblasts and primary rat astrocytes We offer a variety of Gibco MEM modifications for a range of cell culture applications Find the right formulation using the media selector tool This MEM is modified as follows With Without • NEAA • L glutamine • Phenol Red • HEPES The complete formulation is available Gibco MEM developed by Harry Eagle was based on his earlier formulation of Basal Medium Eagle BME Many other modifications of MEM followed including Glasgow s MEM MEM α DMEM and Temin s Modification MEM is available with Earle s salts for use in a CO2 incubator or with Hanks salts for use without CO2 This product is made with Earle s salts Product Intended UseFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law Dual site cGMP Manufacturing and Quality SystemGibco MEM is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards For supply chain continuity we offer an identical Gibco MEM product made in our Scotland facility 10370 047 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard MEM contains no proteins lipids or growth factors Therefore MEM requires supplementation commonly with 10 Fetal Bovine Serum FBS MEM uses a sodium bicarbonate buffer system 2 2 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH
Catalog Number:
10370021
Price:
None
Category:
Cell Culture Transfection Reagents
Applications:
Cell Culture|Mammalian Cell Culture
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Structured Review
Minimum Essential Medium MEM is one of the most commonly used of all cell culture media MEM can be used with a variety of suspension and adherent mammalian cells including HeLa BHK 21 293 HEP 2 HT 1080 MCF 7 fibroblasts and primary rat astrocytes We offer a variety of Gibco MEM modifications for a range of cell culture applications Find the right formulation using the media selector tool This MEM is modified as follows With Without • NEAA • L glutamine • Phenol Red • HEPES The complete formulation is available Gibco MEM developed by Harry Eagle was based on his earlier formulation of Basal Medium Eagle BME Many other modifications of MEM followed including Glasgow s MEM MEM α DMEM and Temin s Modification MEM is available with Earle s salts for use in a CO2 incubator or with Hanks salts for use without CO2 This product is made with Earle s salts Product Intended UseFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law Dual site cGMP Manufacturing and Quality SystemGibco MEM is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards For supply chain continuity we offer an identical Gibco MEM product made in our Scotland facility 10370 047 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard MEM contains no proteins lipids or growth factors Therefore MEM requires supplementation commonly with 10 Fetal Bovine Serum FBS MEM uses a sodium bicarbonate buffer system 2 2 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH
https://www.bioz.com/result/mem neaa/product/Thermo Fisher
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Cell Culture:Article Title: Systematic comparison of hUC-MSCs at various passages reveals the variations of signatures and therapeutic effect on acute graft-versus-host disease Article Snippet: .. The hUC-MSCs (P2, P5, and P14) were simultaneously thawed and cultured in the MSC culture medium (DMEM/F12 basal medium supplemented with 10% fetal bovine serum (Australia), 1% Article Title: Exosomal LGALS9 in the cerebrospinal fluid of glioblastoma patients suppressed dendritic cell antigen presentation and cytotoxic T-cell immunity Article Snippet: GL261 and U118 MG cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) containing 10% fetal calf serum (FCS; Gibco) and 1% penicillin–streptomycin (Life Technologies, Gaithersburg, MD) at 37 °C and 5% CO2 . .. U87 MG cells were cultured in Article Title: Fibroblast-Derived Extracellular Matrices: An Alternative Cell Culture System That Increases Metastatic Cellular Properties Article Snippet: IMR90 and HDF cells were both cultured in RPMI 1640 (Hyclone SH30027.01) supplemented with 10% FBS (Hyclone SH30070.03), 1% penicillin-streptomycin (Hyclone SV30030), and 1% L-glutamine (Hyclone SH30034.01). .. Modification:Article Title: Regulation of hepatic stellate cell proliferation and activation by glutamine metabolism Article Snippet: Reagents and chemicals Carbon tetra-chloride (CCl4 ) was purchased from Merck (Whitehouse Station, NJ). .. Dulbecco's Modified Eagle Medium (DMEM) with and without L-glutamine, α-KG and Mouse Assay:Article Title: CCR8 is expressed by post-positive selection CD4-lineage thymocytes but is dispensable for central tolerance induction Article Snippet: For OT-II chimeras, donor bone marrow from Ccr8 +/+ OT-II or from Ccr8 -/- OT-II mice was transplanted into lethally irradiated RIP-mOVA+ or RIP-mOVA- recipient mice as indicated. .. 106 thymocytes from Ccr8 +/+ or Ccr8 -/- mice were incubated at 37°C, 5% CO2 for 24 hours in 200μl of complete RPMI (RPMI-1640 medium [Gibco] + 10% FBS [Hyclone], 1x GlutaMAX, 1x Penicillin [100U/ml]- Streptomycin [100μg/ml]-Glutamine [300μg/ml], 1mM Sodium Pyruvate, 1x Incubation:Article Title: CCR8 is expressed by post-positive selection CD4-lineage thymocytes but is dispensable for central tolerance induction Article Snippet: For OT-II chimeras, donor bone marrow from Ccr8 +/+ OT-II or from Ccr8 -/- OT-II mice was transplanted into lethally irradiated RIP-mOVA+ or RIP-mOVA- recipient mice as indicated. .. 106 thymocytes from Ccr8 +/+ or Ccr8 -/- mice were incubated at 37°C, 5% CO2 for 24 hours in 200μl of complete RPMI (RPMI-1640 medium [Gibco] + 10% FBS [Hyclone], 1x GlutaMAX, 1x Penicillin [100U/ml]- Streptomycin [100μg/ml]-Glutamine [300μg/ml], 1mM Sodium Pyruvate, 1x |