mem neaa  (Thermo Fisher)


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    Name:
    MEM NEAA no glutamine
    Description:
    Minimum Essential Medium MEM is one of the most commonly used of all cell culture media MEM can be used with a variety of suspension and adherent mammalian cells including HeLa BHK 21 293 HEP 2 HT 1080 MCF 7 fibroblasts and primary rat astrocytes We offer a variety of Gibco MEM modifications for a range of cell culture applications Find the right formulation using the media selector tool This MEM is modified as follows With Without • NEAA • L glutamine • Phenol Red • HEPES The complete formulation is available Gibco MEM developed by Harry Eagle was based on his earlier formulation of Basal Medium Eagle BME Many other modifications of MEM followed including Glasgow s MEM MEM α DMEM and Temin s Modification MEM is available with Earle s salts for use in a CO2 incubator or with Hanks salts for use without CO2 This product is made with Earle s salts Product Intended UseFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law Dual site cGMP Manufacturing and Quality SystemGibco MEM is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards For supply chain continuity we offer an identical Gibco MEM product made in our Scotland facility 10370 047 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard MEM contains no proteins lipids or growth factors Therefore MEM requires supplementation commonly with 10 Fetal Bovine Serum FBS MEM uses a sodium bicarbonate buffer system 2 2 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH
    Catalog Number:
    10370021
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Structured Review

    Thermo Fisher mem neaa
    Minimum Essential Medium MEM is one of the most commonly used of all cell culture media MEM can be used with a variety of suspension and adherent mammalian cells including HeLa BHK 21 293 HEP 2 HT 1080 MCF 7 fibroblasts and primary rat astrocytes We offer a variety of Gibco MEM modifications for a range of cell culture applications Find the right formulation using the media selector tool This MEM is modified as follows With Without • NEAA • L glutamine • Phenol Red • HEPES The complete formulation is available Gibco MEM developed by Harry Eagle was based on his earlier formulation of Basal Medium Eagle BME Many other modifications of MEM followed including Glasgow s MEM MEM α DMEM and Temin s Modification MEM is available with Earle s salts for use in a CO2 incubator or with Hanks salts for use without CO2 This product is made with Earle s salts Product Intended UseFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law Dual site cGMP Manufacturing and Quality SystemGibco MEM is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards For supply chain continuity we offer an identical Gibco MEM product made in our Scotland facility 10370 047 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard MEM contains no proteins lipids or growth factors Therefore MEM requires supplementation commonly with 10 Fetal Bovine Serum FBS MEM uses a sodium bicarbonate buffer system 2 2 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH
    https://www.bioz.com/result/mem neaa/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mem neaa - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Systematic comparison of hUC-MSCs at various passages reveals the variations of signatures and therapeutic effect on acute graft-versus-host disease
    Article Snippet: .. The hUC-MSCs (P2, P5, and P14) were simultaneously thawed and cultured in the MSC culture medium (DMEM/F12 basal medium supplemented with 10% fetal bovine serum (Australia), 1% NEAA (Gibco), 1% l -glutamine (Gibco), 4 ng/ml bFGF (PeproTECH), 4 ng/ml EGF (PeproTECH), 1% penicillin and streptomycin (ThermoFisher)). ..

    Article Title: Exosomal LGALS9 in the cerebrospinal fluid of glioblastoma patients suppressed dendritic cell antigen presentation and cytotoxic T-cell immunity
    Article Snippet: GL261 and U118 MG cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) containing 10% fetal calf serum (FCS; Gibco) and 1% penicillin–streptomycin (Life Technologies, Gaithersburg, MD) at 37 °C and 5% CO2 . .. U87 MG cells were cultured in Minimum Essential Medium (MEM) (Gibco) containing 1% non-essential amino acids (NEAA) (Gibco), 10% fetal bovine serum (Gibco) and 1% penicillin–streptomycin (Life Technologies) at 37 °C and 5% CO2. .. Production of human DCs and T cells from PBMCs Relatively homogeneous functionally mature DC populations can be generated from CD14 + blood monocytes by incubation with appropriate cytokines .

    Article Title: Fibroblast-Derived Extracellular Matrices: An Alternative Cell Culture System That Increases Metastatic Cellular Properties
    Article Snippet: IMR90 and HDF cells were both cultured in RPMI 1640 (Hyclone SH30027.01) supplemented with 10% FBS (Hyclone SH30070.03), 1% penicillin-streptomycin (Hyclone SV30030), and 1% L-glutamine (Hyclone SH30034.01). .. WI38 cells were cultured in MEM media (Gibco 10370–021) supplemented with 10% FBS, 1% penicillin-streptomycin, and 1% L-Glutamine. .. Three-epithelial cell lines, A549, H358, and HPL1D were used to seed onto fibroblast-derived matrix were purchased from ATCC.

    Modification:

    Article Title: Regulation of hepatic stellate cell proliferation and activation by glutamine metabolism
    Article Snippet: Reagents and chemicals Carbon tetra-chloride (CCl4 ) was purchased from Merck (Whitehouse Station, NJ). .. Dulbecco's Modified Eagle Medium (DMEM) with and without L-glutamine, α-KG and NEAA were purchased from Life Technologies (Grand Island, NY). .. Bptes, EGCG, AOAA, 2-DG, 10058-F4, TGF-β1, GDC-0449 and 5-bromo-2'-deoxyuridine (BrdU) were purchased from Sigma-Aldrich (MO, USA).

    Mouse Assay:

    Article Title: CCR8 is expressed by post-positive selection CD4-lineage thymocytes but is dispensable for central tolerance induction
    Article Snippet: For OT-II chimeras, donor bone marrow from Ccr8 +/+ OT-II or from Ccr8 -/- OT-II mice was transplanted into lethally irradiated RIP-mOVA+ or RIP-mOVA- recipient mice as indicated. .. 106 thymocytes from Ccr8 +/+ or Ccr8 -/- mice were incubated at 37°C, 5% CO2 for 24 hours in 200μl of complete RPMI (RPMI-1640 medium [Gibco] + 10% FBS [Hyclone], 1x GlutaMAX, 1x Penicillin [100U/ml]- Streptomycin [100μg/ml]-Glutamine [300μg/ml], 1mM Sodium Pyruvate, 1x MEM NEAA, and 50μM 2-mercaptoethanol [Gibco]), with or without CCL8. .. After 24 hours, cells were immunostained with AnnexinV-PE (eBiosicence) and resuspended in FW + 0.1μg/ml PI (Enzo), and viability (AnnexinV- PI- ) was assessed by flow cytometry.

    Incubation:

    Article Title: CCR8 is expressed by post-positive selection CD4-lineage thymocytes but is dispensable for central tolerance induction
    Article Snippet: For OT-II chimeras, donor bone marrow from Ccr8 +/+ OT-II or from Ccr8 -/- OT-II mice was transplanted into lethally irradiated RIP-mOVA+ or RIP-mOVA- recipient mice as indicated. .. 106 thymocytes from Ccr8 +/+ or Ccr8 -/- mice were incubated at 37°C, 5% CO2 for 24 hours in 200μl of complete RPMI (RPMI-1640 medium [Gibco] + 10% FBS [Hyclone], 1x GlutaMAX, 1x Penicillin [100U/ml]- Streptomycin [100μg/ml]-Glutamine [300μg/ml], 1mM Sodium Pyruvate, 1x MEM NEAA, and 50μM 2-mercaptoethanol [Gibco]), with or without CCL8. .. After 24 hours, cells were immunostained with AnnexinV-PE (eBiosicence) and resuspended in FW + 0.1μg/ml PI (Enzo), and viability (AnnexinV- PI- ) was assessed by flow cytometry.

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  • 99
    Thermo Fisher dmem medium
    25HC does not directly affect bacterial infectivity or host viability. 25HC could inhibit L. monocytogenes infection through different mechanisms. For example, it may (1) directly reduce bacterial viability, (2) inhibit the expression or function of bacterial virulence factors, (3) induce host cell death, or (4) regulate host cellular processes that limit bacterial infection. a , To determine whether 25HC directly reduced bacterial viability, a starting bacterial culture was back-diluted in <t>DMEM</t> (10% FBS) supplemented with vehicle (EtOH) or 25HC (5 μM). Bacterial cultures were incubated at 37°C while shaking at 200 rpm, and OD 600 was measured for each sample at the indicated time points. Mean values from 3 independent experiments are plotted, and error bars show s.d. b , To determine whether 25HC directly modifies bacterial virulence, GFP-expressing L. monocytogenes were cultured overnight in BHI supplemented with vehicle (EtOH) or 25HC (5 μM) and <t>HEK293A</t> cells were then infected with bacteria from either culture (MOI = 20, 6 hours). Infection was analysed by flow cytometry. Bars represent mean values. Error bars show s.d. from three independent experiments and statistical significance was determined by student’s unpaired t-test (two-tailed). c-d , Host cell viability was assessed in cells treated with 25HC (c) or in cells virally transduced with CH25H (d). HEK293A cells were treated with 25HC (5 μM) or vehicle (EtOH) for 6, 16, or 24 hours (c) or transduced with Fluc or CH25H for 72 hours (d). Cell viability was evaluated by measuring ATP production using CellTiter-Glo assays. Data are normalized to vehicle (EtOH) in (c), and Fluc in (d). Bars represent mean values. Error bars show s.d. from three independent experiments and statistical significance was determined before normalization by student’s unpaired t-test (two-tailed).
    Dmem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem medium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem medium - by Bioz Stars, 2021-03
    99/100 stars
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    99
    Thermo Fisher wi38 cells
    Fibroblast-derived ECM induce Directional Migration. (A) Cells were seeded on ECM or fibronectin and placed in a humidified temperature controlled chamber. Fields of cells were photographed every 10 minutes for approximately 8 hours. Resultant time-lapse tracks of A549, H358, and HPL1D on <t>WI38</t> ECM were followed and indicated. (B) Directionality of the cells on different substrates was calculated. N = 7. (C) The average velocity of migrated cells was calculated from the time-lapse microscopy (μm/hour) n = 7. *, p-value ≤ 0.05.
    Wi38 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wi38 cells - by Bioz Stars, 2021-03
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    25HC does not directly affect bacterial infectivity or host viability. 25HC could inhibit L. monocytogenes infection through different mechanisms. For example, it may (1) directly reduce bacterial viability, (2) inhibit the expression or function of bacterial virulence factors, (3) induce host cell death, or (4) regulate host cellular processes that limit bacterial infection. a , To determine whether 25HC directly reduced bacterial viability, a starting bacterial culture was back-diluted in DMEM (10% FBS) supplemented with vehicle (EtOH) or 25HC (5 μM). Bacterial cultures were incubated at 37°C while shaking at 200 rpm, and OD 600 was measured for each sample at the indicated time points. Mean values from 3 independent experiments are plotted, and error bars show s.d. b , To determine whether 25HC directly modifies bacterial virulence, GFP-expressing L. monocytogenes were cultured overnight in BHI supplemented with vehicle (EtOH) or 25HC (5 μM) and HEK293A cells were then infected with bacteria from either culture (MOI = 20, 6 hours). Infection was analysed by flow cytometry. Bars represent mean values. Error bars show s.d. from three independent experiments and statistical significance was determined by student’s unpaired t-test (two-tailed). c-d , Host cell viability was assessed in cells treated with 25HC (c) or in cells virally transduced with CH25H (d). HEK293A cells were treated with 25HC (5 μM) or vehicle (EtOH) for 6, 16, or 24 hours (c) or transduced with Fluc or CH25H for 72 hours (d). Cell viability was evaluated by measuring ATP production using CellTiter-Glo assays. Data are normalized to vehicle (EtOH) in (c), and Fluc in (d). Bars represent mean values. Error bars show s.d. from three independent experiments and statistical significance was determined before normalization by student’s unpaired t-test (two-tailed).

    Journal: Nature microbiology

    Article Title: Oxysterols provide innate immunity to bacterial infection by mobilizing cell surface accessible cholesterol

    doi: 10.1038/s41564-020-0701-5

    Figure Lengend Snippet: 25HC does not directly affect bacterial infectivity or host viability. 25HC could inhibit L. monocytogenes infection through different mechanisms. For example, it may (1) directly reduce bacterial viability, (2) inhibit the expression or function of bacterial virulence factors, (3) induce host cell death, or (4) regulate host cellular processes that limit bacterial infection. a , To determine whether 25HC directly reduced bacterial viability, a starting bacterial culture was back-diluted in DMEM (10% FBS) supplemented with vehicle (EtOH) or 25HC (5 μM). Bacterial cultures were incubated at 37°C while shaking at 200 rpm, and OD 600 was measured for each sample at the indicated time points. Mean values from 3 independent experiments are plotted, and error bars show s.d. b , To determine whether 25HC directly modifies bacterial virulence, GFP-expressing L. monocytogenes were cultured overnight in BHI supplemented with vehicle (EtOH) or 25HC (5 μM) and HEK293A cells were then infected with bacteria from either culture (MOI = 20, 6 hours). Infection was analysed by flow cytometry. Bars represent mean values. Error bars show s.d. from three independent experiments and statistical significance was determined by student’s unpaired t-test (two-tailed). c-d , Host cell viability was assessed in cells treated with 25HC (c) or in cells virally transduced with CH25H (d). HEK293A cells were treated with 25HC (5 μM) or vehicle (EtOH) for 6, 16, or 24 hours (c) or transduced with Fluc or CH25H for 72 hours (d). Cell viability was evaluated by measuring ATP production using CellTiter-Glo assays. Data are normalized to vehicle (EtOH) in (c), and Fluc in (d). Bars represent mean values. Error bars show s.d. from three independent experiments and statistical significance was determined before normalization by student’s unpaired t-test (two-tailed).

    Article Snippet: For the BMDM medium-transfer infection assays ( , and ), 15,000 HEK293A cells were seeded per well of a 96-well plate in DMEM medium (containing 5% LPDS, 1×NEAA).

    Techniques: Infection, Expressing, Incubation, Cell Culture, Flow Cytometry, Two Tailed Test, Transduction

    Fibroblast-derived ECM induce Directional Migration. (A) Cells were seeded on ECM or fibronectin and placed in a humidified temperature controlled chamber. Fields of cells were photographed every 10 minutes for approximately 8 hours. Resultant time-lapse tracks of A549, H358, and HPL1D on WI38 ECM were followed and indicated. (B) Directionality of the cells on different substrates was calculated. N = 7. (C) The average velocity of migrated cells was calculated from the time-lapse microscopy (μm/hour) n = 7. *, p-value ≤ 0.05.

    Journal: PLoS ONE

    Article Title: Fibroblast-Derived Extracellular Matrices: An Alternative Cell Culture System That Increases Metastatic Cellular Properties

    doi: 10.1371/journal.pone.0138065

    Figure Lengend Snippet: Fibroblast-derived ECM induce Directional Migration. (A) Cells were seeded on ECM or fibronectin and placed in a humidified temperature controlled chamber. Fields of cells were photographed every 10 minutes for approximately 8 hours. Resultant time-lapse tracks of A549, H358, and HPL1D on WI38 ECM were followed and indicated. (B) Directionality of the cells on different substrates was calculated. N = 7. (C) The average velocity of migrated cells was calculated from the time-lapse microscopy (μm/hour) n = 7. *, p-value ≤ 0.05.

    Article Snippet: WI38 cells were cultured in MEM media (Gibco 10370–021) supplemented with 10% FBS, 1% penicillin-streptomycin, and 1% L-Glutamine.

    Techniques: Derivative Assay, Migration, Time-lapse Microscopy

    Fibroblast-derived ECM alter Lung Cancer Cell Line Morphology. (A) Phase contrast microscopy photos of A549, H358, and HPL1D cells on FN, WI38 ECM, IMR90, and HDF ECM. (B) Immunofluorescent confocal microscopy of A549 cells on WI38-derived ECM stained with Phallodin and DAPI. (C) The circularity of A549 on FN and 3 different fibroblast-derived ECM was calculated. n = 10. *, p ≤ 0.05.

    Journal: PLoS ONE

    Article Title: Fibroblast-Derived Extracellular Matrices: An Alternative Cell Culture System That Increases Metastatic Cellular Properties

    doi: 10.1371/journal.pone.0138065

    Figure Lengend Snippet: Fibroblast-derived ECM alter Lung Cancer Cell Line Morphology. (A) Phase contrast microscopy photos of A549, H358, and HPL1D cells on FN, WI38 ECM, IMR90, and HDF ECM. (B) Immunofluorescent confocal microscopy of A549 cells on WI38-derived ECM stained with Phallodin and DAPI. (C) The circularity of A549 on FN and 3 different fibroblast-derived ECM was calculated. n = 10. *, p ≤ 0.05.

    Article Snippet: WI38 cells were cultured in MEM media (Gibco 10370–021) supplemented with 10% FBS, 1% penicillin-streptomycin, and 1% L-Glutamine.

    Techniques: Derivative Assay, Microscopy, Confocal Microscopy, Staining

    Fibroblast-derived ECM alters Protein Levels of A549 Cells. (A) A549 cells were cultured on the indicated substrate for 48 hours and then cells were harvested and western blots were performed with the indicated antibodies. (B) Cells were treated as in panel A and westerns were performed with indicated antibodies. (C) A549 cells were seeded on either fibronectin (FN) or WI38-derived ECM and then plates were incubated under hypoxic or normoxic conditions. Cells were harvested and westerns were performed with the indicated antibodies.

    Journal: PLoS ONE

    Article Title: Fibroblast-Derived Extracellular Matrices: An Alternative Cell Culture System That Increases Metastatic Cellular Properties

    doi: 10.1371/journal.pone.0138065

    Figure Lengend Snippet: Fibroblast-derived ECM alters Protein Levels of A549 Cells. (A) A549 cells were cultured on the indicated substrate for 48 hours and then cells were harvested and western blots were performed with the indicated antibodies. (B) Cells were treated as in panel A and westerns were performed with indicated antibodies. (C) A549 cells were seeded on either fibronectin (FN) or WI38-derived ECM and then plates were incubated under hypoxic or normoxic conditions. Cells were harvested and westerns were performed with the indicated antibodies.

    Article Snippet: WI38 cells were cultured in MEM media (Gibco 10370–021) supplemented with 10% FBS, 1% penicillin-streptomycin, and 1% L-Glutamine.

    Techniques: Derivative Assay, Cell Culture, Western Blot, Incubation

    Fibroblast-derived ECM Protects Lung Cancer Cell Lines from Serum Deprivation. (A) A549, H358, and HPL1D cells were grown on fibronectin (FN) or on WI38 ECM in serum-free media for 48 hours and then photographed. (B) Relative cell numbers were quantified for A549 cells (B), H358 cells (C) and HPL1D cells (D) using Alamar Blue every 24 hours after cells were put into serum free media. By the fourth day in serum free media, basically all cells were dead.

    Journal: PLoS ONE

    Article Title: Fibroblast-Derived Extracellular Matrices: An Alternative Cell Culture System That Increases Metastatic Cellular Properties

    doi: 10.1371/journal.pone.0138065

    Figure Lengend Snippet: Fibroblast-derived ECM Protects Lung Cancer Cell Lines from Serum Deprivation. (A) A549, H358, and HPL1D cells were grown on fibronectin (FN) or on WI38 ECM in serum-free media for 48 hours and then photographed. (B) Relative cell numbers were quantified for A549 cells (B), H358 cells (C) and HPL1D cells (D) using Alamar Blue every 24 hours after cells were put into serum free media. By the fourth day in serum free media, basically all cells were dead.

    Article Snippet: WI38 cells were cultured in MEM media (Gibco 10370–021) supplemented with 10% FBS, 1% penicillin-streptomycin, and 1% L-Glutamine.

    Techniques: Derivative Assay

    The phenotypic characterization of hUC-MSCs at various passages. a Phase contrast images of hUC-MSCs at various passages (P3, P6, P15) in DMEM/F12 medium containing 10% FBS, 1% l -glutamine, 1% NEAA, and 1% penicillin-streptomycin (short for 10% FBS/DF12 medium thereafter). Scale bar = 200 μm. b Flow cytometry (FCM) analysis of MSC markers of hUC-MSCs at various passages (P3, P6, P15) cultured in 10% FBS/DF12 medium (mean ± SEM, n = 3). NS, not significant. c Proliferation assay of hUC-MSCs at various passages (P3, P6, P15) cultured in 10% FBS/DF12 medium for 7 days (mean ± SEM, n = 3). * P

    Journal: Stem Cell Research & Therapy

    Article Title: Systematic comparison of hUC-MSCs at various passages reveals the variations of signatures and therapeutic effect on acute graft-versus-host disease

    doi: 10.1186/s13287-019-1478-4

    Figure Lengend Snippet: The phenotypic characterization of hUC-MSCs at various passages. a Phase contrast images of hUC-MSCs at various passages (P3, P6, P15) in DMEM/F12 medium containing 10% FBS, 1% l -glutamine, 1% NEAA, and 1% penicillin-streptomycin (short for 10% FBS/DF12 medium thereafter). Scale bar = 200 μm. b Flow cytometry (FCM) analysis of MSC markers of hUC-MSCs at various passages (P3, P6, P15) cultured in 10% FBS/DF12 medium (mean ± SEM, n = 3). NS, not significant. c Proliferation assay of hUC-MSCs at various passages (P3, P6, P15) cultured in 10% FBS/DF12 medium for 7 days (mean ± SEM, n = 3). * P

    Article Snippet: The hUC-MSCs (P2, P5, and P14) were simultaneously thawed and cultured in the MSC culture medium (DMEM/F12 basal medium supplemented with 10% fetal bovine serum (Australia), 1% NEAA (Gibco), 1% l -glutamine (Gibco), 4 ng/ml bFGF (PeproTECH), 4 ng/ml EGF (PeproTECH), 1% penicillin and streptomycin (ThermoFisher)).

    Techniques: Flow Cytometry, Cytometry, Cell Culture, Proliferation Assay

    Isolation and phenotype of SCAP-Ss and DPSCs. (a) Age distribution of the patients with supernumerary teeth during the years of 2016–2018 at the dental clinic of the First Affiliated Hospital of Fujian Medical University. (b) The periapical radiography of the supernumerary teeth (mesiodens) and permanent teeth of the patients in this study. (c) The removed supernumerary tooth and permanent tooth in a 10 cm dish. (d) Phase contrast images of primary (upper panel) and passages (bottom panel) of SCAP-Ss and DPSCs derived from the supernumerary tooth and permanent tooth (P1, P3), respectively. Scale bar = 200 μ m (upper panel) or scale bar = 100 μ m (bottom panel). (e) Flow cytometry (FCM) analysis for surface markers (CD73, CD90, CD105, CD44, CD31, CD34, CD45, and HLA-DR) of SCAP-Ss and DPSCs in DMEM/F12 basal medium supplemented with 1% NEAA (Gibco), 1% L-glutamine (Gibco), 1% Penicillin and streptomycin (ThermoFisher), 10% fetal bovine serum, 4 ng/ml EGF, and 4 ng/ml bFGF. (f) Statistical analysis of FCM data in (e). All data were shown as the mean ± SEM ( N = 3). NS: not significant.

    Journal: Stem Cells International

    Article Title: Human Supernumerary Teeth-Derived Apical Papillary Stem Cells Possess Preferable Characteristics and Efficacy on Hepatic Fibrosis in Mice

    doi: 10.1155/2020/6489396

    Figure Lengend Snippet: Isolation and phenotype of SCAP-Ss and DPSCs. (a) Age distribution of the patients with supernumerary teeth during the years of 2016–2018 at the dental clinic of the First Affiliated Hospital of Fujian Medical University. (b) The periapical radiography of the supernumerary teeth (mesiodens) and permanent teeth of the patients in this study. (c) The removed supernumerary tooth and permanent tooth in a 10 cm dish. (d) Phase contrast images of primary (upper panel) and passages (bottom panel) of SCAP-Ss and DPSCs derived from the supernumerary tooth and permanent tooth (P1, P3), respectively. Scale bar = 200 μ m (upper panel) or scale bar = 100 μ m (bottom panel). (e) Flow cytometry (FCM) analysis for surface markers (CD73, CD90, CD105, CD44, CD31, CD34, CD45, and HLA-DR) of SCAP-Ss and DPSCs in DMEM/F12 basal medium supplemented with 1% NEAA (Gibco), 1% L-glutamine (Gibco), 1% Penicillin and streptomycin (ThermoFisher), 10% fetal bovine serum, 4 ng/ml EGF, and 4 ng/ml bFGF. (f) Statistical analysis of FCM data in (e). All data were shown as the mean ± SEM ( N = 3). NS: not significant.

    Article Snippet: Briefly, the two types of stem cells were maintained in DMEM/F12 basal medium supplemented with 1% NEAA (Gibco), 1% L-glutamine (Gibco), 1% Penicillin and streptomycin (ThermoFisher), 10% fetal bovine serum (Australia), 4 ng/ml EGF (PeproTECH), and 4 ng/ml bFGF (PeproTECH).

    Techniques: Isolation, Derivative Assay, Flow Cytometry