Structured Review

TCI Tokyo Chemical Industry mehp
In vitro activation of PPARα and PPARγ by TBPH and TBMEHP. Cell lysates from NIH 3T3 L1 cells treated for 7 days with <t>MEHP</t> (50–100 μM), or <t>rosiglitazone</t> (Rosi; 100 nM), TBPH, TBMEHP or 0.1% DMSO (vehicle) in the presence of insulin (0.5 μg/mL) and dexamethasone (10 –9 M) were stained with Nile Red and their fluorescence measured to quantify lipid accumulation ( A–C ) and immunoblotted for perilipin expression ( D ). RFU, relative fluorescence units. Gene expression analysis of FABPB4 in NIH 3T3 L1 cells ( E ) and AOX in FAO-PPARα cells ( F ) treated with TBPH or TBMEHP for 24 hr was performed using qPCR. Data (mean ± SE) are representative of at least three independent experiments. ** p
Mehp, supplied by TCI Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mehp/product/TCI Tokyo Chemical Industry
Average 91 stars, based on 2 article reviews
Price from $9.99 to $1999.99
mehp - by Bioz Stars, 2020-09
91/100 stars

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1) Product Images from "Rodent Thyroid, Liver, and Fetal Testis Toxicity of the Monoester Metabolite of Bis-(2-ethylhexyl) Tetrabromophthalate (TBPH), a Novel Brominated Flame Retardant Present in Indoor Dust"

Article Title: Rodent Thyroid, Liver, and Fetal Testis Toxicity of the Monoester Metabolite of Bis-(2-ethylhexyl) Tetrabromophthalate (TBPH), a Novel Brominated Flame Retardant Present in Indoor Dust

Journal: Environmental Health Perspectives

doi: 10.1289/ehp.1204932

In vitro activation of PPARα and PPARγ by TBPH and TBMEHP. Cell lysates from NIH 3T3 L1 cells treated for 7 days with MEHP (50–100 μM), or rosiglitazone (Rosi; 100 nM), TBPH, TBMEHP or 0.1% DMSO (vehicle) in the presence of insulin (0.5 μg/mL) and dexamethasone (10 –9 M) were stained with Nile Red and their fluorescence measured to quantify lipid accumulation ( A–C ) and immunoblotted for perilipin expression ( D ). RFU, relative fluorescence units. Gene expression analysis of FABPB4 in NIH 3T3 L1 cells ( E ) and AOX in FAO-PPARα cells ( F ) treated with TBPH or TBMEHP for 24 hr was performed using qPCR. Data (mean ± SE) are representative of at least three independent experiments. ** p
Figure Legend Snippet: In vitro activation of PPARα and PPARγ by TBPH and TBMEHP. Cell lysates from NIH 3T3 L1 cells treated for 7 days with MEHP (50–100 μM), or rosiglitazone (Rosi; 100 nM), TBPH, TBMEHP or 0.1% DMSO (vehicle) in the presence of insulin (0.5 μg/mL) and dexamethasone (10 –9 M) were stained with Nile Red and their fluorescence measured to quantify lipid accumulation ( A–C ) and immunoblotted for perilipin expression ( D ). RFU, relative fluorescence units. Gene expression analysis of FABPB4 in NIH 3T3 L1 cells ( E ) and AOX in FAO-PPARα cells ( F ) treated with TBPH or TBMEHP for 24 hr was performed using qPCR. Data (mean ± SE) are representative of at least three independent experiments. ** p

Techniques Used: In Vitro, Activation Assay, Staining, Fluorescence, Expressing, Real-time Polymerase Chain Reaction

Related Articles

other:

Article Title: Inflammatory Effects Of Phthalates In Neonatal Neutrophils
Article Snippet: MEHP was from TCI America (Portland, OR) and Amplex Red and horseradish peroxidase from Molecular Probes (Carlsbad, CA).

Article Title: Influence of TRP53 status on FAS membrane localization, CFLAR (c-FLIP) ubiquitinylation, and sensitivity of GC-2spd (ts) cells to undergo FAS-mediated apoptosis.
Article Snippet: TreatmentsGC-2 cells that had been in culture for 48 h (grown at either temperature) were exposed to various treatments including JO2 (anti mouse-FAS antibody, 554254; Pharmingen, San Diego, CA), hamster IgG (Isotype control for JO2, 553961; Pharmingen), MEHP (TCI America, Portland, OR), or DMSO (Sigma Aldrich, San Diego, CA) for the indicated time periods.

Article Title: Reproductive Effects in F1 Adult Females Exposed In Utero to Moderate to High Doses of Mono-2-ethylhexylphthalate (MEHP)
Article Snippet: At gestational days 17, 18, and 19 (with day of plug detection considered GD1), pregnant dams were exposed via oral gavage to 100, 500, or 1000 mg/kg MEHP (TCI America) dissolved in corn oil, or an equivalent volume of corn oil as a vehicle control.

Gas Chromatography:

Article Title: Cross-talk between the Akt and NF-?B Signaling Pathways Inhibits MEHP-Induced Germ Cell Apoptosis
Article Snippet: .. MEHP was purchased from TCI America (Portland, OR) and certified to be more than 94% pure by gas chromatography. .. Akt1 wild-type, Akt1 heterozygous, and Akt1-deficient mice were gavaged at postnatal day 28.

Mouse Assay:

Article Title: Transcriptional Suppression of Sertoli Cell Timp2 in Rodents Following Mono-(2-ethylhexyl) Phthalate Exposure Is Regulated by CEBPA and MYC 1
Article Snippet: .. Mice (28-day-old) were given a single dose (1 g/kg, in corn oil) of MEHP (TCI America, Portland, OR) by oral gavage, an established model system for eliciting Sertoli cell dysfunction [ ]. ..

Article Title: FasL Gene–Deficient Mice Display a Limited Disruption in Spermatogenesis and Inhibition of Mono-(2-ethylhexyl) Phthalate–Induced Germ Cell Apoptosis
Article Snippet: .. PND 28 FasL−/− and C57BL/6J mice were given a single dose of MEHP (1 g/kg, TCI America, Portland, OR) by oral gavage at a volume equal to 4 ml/kg, a standard procedure for investigating the consequences of MEHP-induced Sertoli cell injury ( ). ..

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    TCI Tokyo Chemical Industry mehp
    In vitro activation of PPARα and PPARγ by TBPH and TBMEHP. Cell lysates from NIH 3T3 L1 cells treated for 7 days with <t>MEHP</t> (50–100 μM), or <t>rosiglitazone</t> (Rosi; 100 nM), TBPH, TBMEHP or 0.1% DMSO (vehicle) in the presence of insulin (0.5 μg/mL) and dexamethasone (10 –9 M) were stained with Nile Red and their fluorescence measured to quantify lipid accumulation ( A–C ) and immunoblotted for perilipin expression ( D ). RFU, relative fluorescence units. Gene expression analysis of FABPB4 in NIH 3T3 L1 cells ( E ) and AOX in FAO-PPARα cells ( F ) treated with TBPH or TBMEHP for 24 hr was performed using qPCR. Data (mean ± SE) are representative of at least three independent experiments. ** p
    Mehp, supplied by TCI Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mehp/product/TCI Tokyo Chemical Industry
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mehp - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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    In vitro activation of PPARα and PPARγ by TBPH and TBMEHP. Cell lysates from NIH 3T3 L1 cells treated for 7 days with MEHP (50–100 μM), or rosiglitazone (Rosi; 100 nM), TBPH, TBMEHP or 0.1% DMSO (vehicle) in the presence of insulin (0.5 μg/mL) and dexamethasone (10 –9 M) were stained with Nile Red and their fluorescence measured to quantify lipid accumulation ( A–C ) and immunoblotted for perilipin expression ( D ). RFU, relative fluorescence units. Gene expression analysis of FABPB4 in NIH 3T3 L1 cells ( E ) and AOX in FAO-PPARα cells ( F ) treated with TBPH or TBMEHP for 24 hr was performed using qPCR. Data (mean ± SE) are representative of at least three independent experiments. ** p

    Journal: Environmental Health Perspectives

    Article Title: Rodent Thyroid, Liver, and Fetal Testis Toxicity of the Monoester Metabolite of Bis-(2-ethylhexyl) Tetrabromophthalate (TBPH), a Novel Brominated Flame Retardant Present in Indoor Dust

    doi: 10.1289/ehp.1204932

    Figure Lengend Snippet: In vitro activation of PPARα and PPARγ by TBPH and TBMEHP. Cell lysates from NIH 3T3 L1 cells treated for 7 days with MEHP (50–100 μM), or rosiglitazone (Rosi; 100 nM), TBPH, TBMEHP or 0.1% DMSO (vehicle) in the presence of insulin (0.5 μg/mL) and dexamethasone (10 –9 M) were stained with Nile Red and their fluorescence measured to quantify lipid accumulation ( A–C ) and immunoblotted for perilipin expression ( D ). RFU, relative fluorescence units. Gene expression analysis of FABPB4 in NIH 3T3 L1 cells ( E ) and AOX in FAO-PPARα cells ( F ) treated with TBPH or TBMEHP for 24 hr was performed using qPCR. Data (mean ± SE) are representative of at least three independent experiments. ** p

    Article Snippet: We purchased MEHP from TCI America (Portland, OR); rosiglitazone from Cayman Chemical (Ann Arbor, MI); and 2-(4-(2-(1-(cyclohexanebutyl)-3-cyclohexylureido)ethyl)phenylthio)-2-methylpropionic acid (GW7647), a prototypic PPARα agonist, was purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: In Vitro, Activation Assay, Staining, Fluorescence, Expressing, Real-time Polymerase Chain Reaction