Structured Review

FUJIFILM mehp
<t>MEHP</t> exposure also induces neutrophil infiltration into the testis (a) Representative images of (A) hematoxylin and eosin staining at 12h and (B) myeloperoxidase staining (brown) of Fisher rats 12, 24, and 48 hours after receiving an oral dose of <t>0.7g/kg</t> MEHP or equivalent volume corn oil. (A) Cells showing multilobular nuclear staining typical of neutrophils are indicated by an arrow in the highlighted box. (C) Summarized data of myeloperoxidase staining. Data are means ± S.E.M for n≥3. Statistical analysis was performed by comparing MPO cell counts of MEHP-exposed animals for each time point with those of controls using one-way ANOVA and Tukey’s multiple comparisons test. **P
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Images

1) Product Images from "MEHP-Induced Rat Testicular Inflammation Does Not Exacerbate Germ Cell Apoptosis"

Article Title: MEHP-Induced Rat Testicular Inflammation Does Not Exacerbate Germ Cell Apoptosis

Journal: Reproduction (Cambridge, England)

doi: 10.1530/REP-18-0093

MEHP exposure also induces neutrophil infiltration into the testis (a) Representative images of (A) hematoxylin and eosin staining at 12h and (B) myeloperoxidase staining (brown) of Fisher rats 12, 24, and 48 hours after receiving an oral dose of 0.7g/kg MEHP or equivalent volume corn oil. (A) Cells showing multilobular nuclear staining typical of neutrophils are indicated by an arrow in the highlighted box. (C) Summarized data of myeloperoxidase staining. Data are means ± S.E.M for n≥3. Statistical analysis was performed by comparing MPO cell counts of MEHP-exposed animals for each time point with those of controls using one-way ANOVA and Tukey’s multiple comparisons test. **P
Figure Legend Snippet: MEHP exposure also induces neutrophil infiltration into the testis (a) Representative images of (A) hematoxylin and eosin staining at 12h and (B) myeloperoxidase staining (brown) of Fisher rats 12, 24, and 48 hours after receiving an oral dose of 0.7g/kg MEHP or equivalent volume corn oil. (A) Cells showing multilobular nuclear staining typical of neutrophils are indicated by an arrow in the highlighted box. (C) Summarized data of myeloperoxidase staining. Data are means ± S.E.M for n≥3. Statistical analysis was performed by comparing MPO cell counts of MEHP-exposed animals for each time point with those of controls using one-way ANOVA and Tukey’s multiple comparisons test. **P

Techniques Used: Staining

2) Product Images from "Mono-(2-Ethylhexyl) Phthalate (MEHP) Promotes Invasion and Migration of Human Testicular Embryonal Carcinoma Cells 1"

Article Title: Mono-(2-Ethylhexyl) Phthalate (MEHP) Promotes Invasion and Migration of Human Testicular Embryonal Carcinoma Cells 1

Journal: Biology of Reproduction

doi: 10.1095/biolreprod.111.097295

MMP2 protein expression and activity in NT2/D1 cells are increased by MEHP exposure. A ) Total protein from NT2/D1 cells treated with or without MEHP were analyzed by Western blot analysis. Time- and dose-dependent induction of MMP2 were detected following
Figure Legend Snippet: MMP2 protein expression and activity in NT2/D1 cells are increased by MEHP exposure. A ) Total protein from NT2/D1 cells treated with or without MEHP were analyzed by Western blot analysis. Time- and dose-dependent induction of MMP2 were detected following

Techniques Used: Expressing, Activity Assay, Western Blot

SB-3CT inhibited MEHP-induced MMP2 activation in NT2/D1 cells. NT2/D1 cells were treated with various doses of SB-3CT and 200 μM of MEHP for 12 h, and conditioned medium was collected. A ) The amount of soluble MMP2 released from NT2/D1 cells was
Figure Legend Snippet: SB-3CT inhibited MEHP-induced MMP2 activation in NT2/D1 cells. NT2/D1 cells were treated with various doses of SB-3CT and 200 μM of MEHP for 12 h, and conditioned medium was collected. A ) The amount of soluble MMP2 released from NT2/D1 cells was

Techniques Used: Activation Assay

Microarray analysis of the gene expression profile in NT2/D1 cells treated or untreated with MEHP. Microarray analysis was performed using human whole-genome OneArray chips version 5 (Phalanx Biotech Group), carrying 29 187 human genome probes
Figure Legend Snippet: Microarray analysis of the gene expression profile in NT2/D1 cells treated or untreated with MEHP. Microarray analysis was performed using human whole-genome OneArray chips version 5 (Phalanx Biotech Group), carrying 29 187 human genome probes

Techniques Used: Microarray, Expressing

That invasion and migration activities of NT2/D1 cells were enhanced by MEHP exposure and suppressed by SB-3CT shows the compensatory effect on MEHP treatment. A ) In vitro invasion assays were performed using Transwell chambers coated with Matrigel. Nontreated,
Figure Legend Snippet: That invasion and migration activities of NT2/D1 cells were enhanced by MEHP exposure and suppressed by SB-3CT shows the compensatory effect on MEHP treatment. A ) In vitro invasion assays were performed using Transwell chambers coated with Matrigel. Nontreated,

Techniques Used: Migration, In Vitro

3) Product Images from "Mono-(2-ethylhexyl) phthalate-induced Sertoli cell injury stimulates the production of pro-inflammatory cytokines in Fisher 344 rats"

Article Title: Mono-(2-ethylhexyl) phthalate-induced Sertoli cell injury stimulates the production of pro-inflammatory cytokines in Fisher 344 rats

Journal: Reproductive toxicology (Elmsford, N.Y.)

doi: 10.1016/j.reprotox.2017.02.013

No change in IL-1α, but MCP-1 does increase in rat Sertoli cell line ASC-17D (A) IL-1α and (B) MCP-1 mRNA expression by ASC-17D SC line at 3, 6, 12 and 24 h after MEHP (200 μM, black) or control (DMSO, grey) exposure (n=3 per treatment per time point). No statistical differences were found in IL-1α mRNA expression. MEHP induced a significant increase in MCP-1 mRNA compared to control at 12 h. Statistical analysis was performed using two-way ANOVA for matched pairs with Sidak’s multiple comparison test and each MEHP-treated sample was compared to its time-point control.
Figure Legend Snippet: No change in IL-1α, but MCP-1 does increase in rat Sertoli cell line ASC-17D (A) IL-1α and (B) MCP-1 mRNA expression by ASC-17D SC line at 3, 6, 12 and 24 h after MEHP (200 μM, black) or control (DMSO, grey) exposure (n=3 per treatment per time point). No statistical differences were found in IL-1α mRNA expression. MEHP induced a significant increase in MCP-1 mRNA compared to control at 12 h. Statistical analysis was performed using two-way ANOVA for matched pairs with Sidak’s multiple comparison test and each MEHP-treated sample was compared to its time-point control.

Techniques Used: Expressing

IL-6 increases in Sertoli cell rat line ASC-17D cells after in vitro exposure to MEHP (A) IL-6 expression in control (0.04% DMSO, control, grey) or MEHP (200 μM, black) treated ASC-17D cells at 3, 6, 12 and 24 h. MEHP induced a significant increase of IL-6 mRNA levels at 12 h. Statistical analysis was performed using two-way ANOVA for matched pairs with Sidak’s multiple comparison test and each MEHP-treated sample was compared to its time-point control. Representative microphotographs (B) of ASC-17D SC line immunostained with IL-6 (green, AlexaFlour488) after exposure to C (DMSO, control) or M (MEHP) for 24 h.
Figure Legend Snippet: IL-6 increases in Sertoli cell rat line ASC-17D cells after in vitro exposure to MEHP (A) IL-6 expression in control (0.04% DMSO, control, grey) or MEHP (200 μM, black) treated ASC-17D cells at 3, 6, 12 and 24 h. MEHP induced a significant increase of IL-6 mRNA levels at 12 h. Statistical analysis was performed using two-way ANOVA for matched pairs with Sidak’s multiple comparison test and each MEHP-treated sample was compared to its time-point control. Representative microphotographs (B) of ASC-17D SC line immunostained with IL-6 (green, AlexaFlour488) after exposure to C (DMSO, control) or M (MEHP) for 24 h.

Techniques Used: In Vitro, Expressing

Related Articles

Concentration Assay:

Article Title: Mono-(2-ethylhexyl) phthalate-induced Sertoli cell injury stimulates the production of pro-inflammatory cytokines in Fisher 344 rats
Article Snippet: .. Primary co-cultures or ASC-17D cells were treated with 200 μM MEHP (97.3% purity; Wako Chemicals, Richmond, VA) [ , ] diluted in dimethyl sulfoxide (DMSO, final concentration: 0.04%) or equivalent volume of DMSO only, for various time periods (3, 6, 12, 24 h) that precede peak CD11b+ infiltration (24h) [ ]. .. Determination of the dose was based on previous experiments that utilized 200 μM MEHP in vitro to replicate the high exposure of MEHP in vivo [ – ].

other:

Article Title: Mono-(2-Ethylhexyl) Phthalate (MEHP) Promotes Invasion and Migration of Human Testicular Embryonal Carcinoma Cells 1
Article Snippet: NT2/D1 cells were treated with designated concentrations of MEHP (Wako Chemicals USA, Inc., Richmond, VA) diluted in dimethyl sulfoxide (DMSO) for various time periods.

Labeling:

Article Title: Decreased Serum Free Testosterone in Workers Exposed to High Levels of Di-n-butyl Phthalate (DBP) and Di-2-ethylhexyl Phthalate (DEHP): A Cross-Sectional Study in China
Article Snippet: .. We purchased MBP, MEHP, and d4 -labeled internal standards from Hayashi Pure Chemical Industries (Osaka, Japan). .. Organic solvents for PE analysis and sample stock preparation were obtained from Kanto Chemical Co. (Tokyo, Japan).