Structured Review

STAGO GmbH megamix plus ssc beads
Megamix Plus Ssc Beads, supplied by STAGO GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix plus ssc beads/product/STAGO GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix plus ssc beads - by Bioz Stars, 2024-03
86/100 stars

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Structured Review

STAGO GmbH megamix plus ssc beads
Quantification of red blood cell-derived microparticles (RMP). ( A – E ) Flow cytometer (FC) settings and gating strategy for RMP quantification with <t>Megamix-Plus</t> <t>SSC</t> and MP-Counting Beads (0.3 µm sized PE fluorescent beads). Shown are density/dot blots from one representative measurement out of 32 independent analyses. SSC-H vs. FITC-H ( A ) and SSC-H vs. FSC-H ( B ) density blot showing the location of the Megamix-Plus SSC beads. Beads were used to set up the SSC-H, FITC-H and FSC-H PMT voltage ( A , B ), the MP gates ( B ) and the SSC threshold for RMP analysis. ( C ) Gating strategy to characterize the RMP population. Dual APC/Annexin V vs. BV450/CD235a scatter dot showing the double stained RMP isolate. RMPs are defined as CD235a and Annexin V positive. ( D ) Dual-APC-H vs. -PE-H density blot showing the location of the MP-Count Beads during sample aquisition. Beads with a known concentration were spiked into each RMP sample. Samples were analyzed at low speed for at least 2 min or to a corresponding minimum of 500 MP-Count Beads. ( E ) SSC-H vs. FSC-H dot blot illustrating the double stained RMP isolate within the prior defined (see ( B )) MP gates. ( F ) Nanoparticle tracking analysis (NTA) of RMP isolates via Nanosight NS500 ( n = 4). Shown is one representative NTA out of four independent analyses of RMP isolates.
Megamix Plus Ssc Beads, supplied by STAGO GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix plus ssc beads/product/STAGO GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix plus ssc beads - by Bioz Stars, 2024-03
86/100 stars

Images

1) Product Images from "Red Blood Cell-Derived Microparticles Exert No Cancer Promoting Effects on Colorectal Cancer Cells In Vitro"

Article Title: Red Blood Cell-Derived Microparticles Exert No Cancer Promoting Effects on Colorectal Cancer Cells In Vitro

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms23169323

Quantification of red blood cell-derived microparticles (RMP). ( A – E ) Flow cytometer (FC) settings and gating strategy for RMP quantification with Megamix-Plus SSC and MP-Counting Beads (0.3 µm sized PE fluorescent beads). Shown are density/dot blots from one representative measurement out of 32 independent analyses. SSC-H vs. FITC-H ( A ) and SSC-H vs. FSC-H ( B ) density blot showing the location of the Megamix-Plus SSC beads. Beads were used to set up the SSC-H, FITC-H and FSC-H PMT voltage ( A , B ), the MP gates ( B ) and the SSC threshold for RMP analysis. ( C ) Gating strategy to characterize the RMP population. Dual APC/Annexin V vs. BV450/CD235a scatter dot showing the double stained RMP isolate. RMPs are defined as CD235a and Annexin V positive. ( D ) Dual-APC-H vs. -PE-H density blot showing the location of the MP-Count Beads during sample aquisition. Beads with a known concentration were spiked into each RMP sample. Samples were analyzed at low speed for at least 2 min or to a corresponding minimum of 500 MP-Count Beads. ( E ) SSC-H vs. FSC-H dot blot illustrating the double stained RMP isolate within the prior defined (see ( B )) MP gates. ( F ) Nanoparticle tracking analysis (NTA) of RMP isolates via Nanosight NS500 ( n = 4). Shown is one representative NTA out of four independent analyses of RMP isolates.
Figure Legend Snippet: Quantification of red blood cell-derived microparticles (RMP). ( A – E ) Flow cytometer (FC) settings and gating strategy for RMP quantification with Megamix-Plus SSC and MP-Counting Beads (0.3 µm sized PE fluorescent beads). Shown are density/dot blots from one representative measurement out of 32 independent analyses. SSC-H vs. FITC-H ( A ) and SSC-H vs. FSC-H ( B ) density blot showing the location of the Megamix-Plus SSC beads. Beads were used to set up the SSC-H, FITC-H and FSC-H PMT voltage ( A , B ), the MP gates ( B ) and the SSC threshold for RMP analysis. ( C ) Gating strategy to characterize the RMP population. Dual APC/Annexin V vs. BV450/CD235a scatter dot showing the double stained RMP isolate. RMPs are defined as CD235a and Annexin V positive. ( D ) Dual-APC-H vs. -PE-H density blot showing the location of the MP-Count Beads during sample aquisition. Beads with a known concentration were spiked into each RMP sample. Samples were analyzed at low speed for at least 2 min or to a corresponding minimum of 500 MP-Count Beads. ( E ) SSC-H vs. FSC-H dot blot illustrating the double stained RMP isolate within the prior defined (see ( B )) MP gates. ( F ) Nanoparticle tracking analysis (NTA) of RMP isolates via Nanosight NS500 ( n = 4). Shown is one representative NTA out of four independent analyses of RMP isolates.

Techniques Used: Derivative Assay, Flow Cytometry, Staining, Concentration Assay, Dot Blot


Structured Review

STAGO GmbH megamix beads
Megamix Beads, supplied by STAGO GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix beads/product/STAGO GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix beads - by Bioz Stars, 2024-03
86/100 stars

Images


Structured Review

STAGO GmbH megamix beads
Megamix Beads, supplied by STAGO GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix beads/product/STAGO GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix beads - by Bioz Stars, 2024-03
86/100 stars

Images


Structured Review

STAGO GmbH megamix beads
Megamix Beads, supplied by STAGO GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix beads/product/STAGO GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix beads - by Bioz Stars, 2024-03
86/100 stars

Images


Structured Review

STAGO GmbH megamix beads
Megamix Beads, supplied by STAGO GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix beads/product/STAGO GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix beads - by Bioz Stars, 2024-03
86/100 stars

Images

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    STAGO GmbH megamix plus ssc beads
    Megamix Plus Ssc Beads, supplied by STAGO GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megamix plus ssc beads/product/STAGO GmbH
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    megamix plus ssc beads - by Bioz Stars, 2024-03
    86/100 stars
      Buy from Supplier

    86
    STAGO GmbH megamix beads
    Megamix Beads, supplied by STAGO GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megamix beads/product/STAGO GmbH
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    megamix beads - by Bioz Stars, 2024-03
    86/100 stars
      Buy from Supplier

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