Structured Review

Becton Dickinson megamix beads
Megamix Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix beads/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix beads - by Bioz Stars, 2024-02
86/100 stars

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Structured Review

Becton Dickinson megamix beads
Megamix Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix beads/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix beads - by Bioz Stars, 2024-02
86/100 stars

Images


Structured Review

Becton Dickinson megamix beads
Megamix Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix beads/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix beads - by Bioz Stars, 2024-02
86/100 stars

Images


Structured Review

Becton Dickinson megamix beads
Megamix Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix beads/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix beads - by Bioz Stars, 2024-02
86/100 stars

Images


Structured Review

Becton Dickinson megamix beads
Visualization of fluorescent EEVs by Flow Virometry analysis. A Physical, FSC vs. Violet SSC plot (left panel), and fluorescence, B525/40 (green) vs. Violet SSC plot (right panel), characterisation of <t>Megamix</t> beads (900 nm—red, 500 nm—blue, 300 nm—yellow and 100 nm—green dots) by BC CytoFLEX S analysis, setting the instrument for flow virometry. B CEFs were infected with either rMVA-77-SHAG or rMVA-77-G- M1-V5, and supernatants were harvested 30 h p.i. Supernatants were analysed with the settings established using the Megamix beads (B525/40 vs. Violet SSC), revealing signals for entities with the “stable green” surface marker (SHAG-EEVs, left panel), but not so for the “cytoplasmic green” marker (G-EEVs, middle panel), unless these entities were stained in the DNA with the SYTO 13 dye (G-EEVs + SYTO 13, right panel). C and D BC CytoFLEX S analysis with B 525/40 (green) versus R 660/20 (red) settings of SHAG-EEVs. The green fluorescence is progressively lost with sample dilution. E) F/T/S treatment of SHAG EEVs abolishes the green signal, confirming its membrane localization
Megamix Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix beads/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix beads - by Bioz Stars, 2024-02
86/100 stars

Images

1) Product Images from "Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting"

Article Title: Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting

Journal: Journal of Translational Medicine

doi: 10.1186/s12967-023-04353-7

Visualization of fluorescent EEVs by Flow Virometry analysis. A Physical, FSC vs. Violet SSC plot (left panel), and fluorescence, B525/40 (green) vs. Violet SSC plot (right panel), characterisation of Megamix beads (900 nm—red, 500 nm—blue, 300 nm—yellow and 100 nm—green dots) by BC CytoFLEX S analysis, setting the instrument for flow virometry. B CEFs were infected with either rMVA-77-SHAG or rMVA-77-G- M1-V5, and supernatants were harvested 30 h p.i. Supernatants were analysed with the settings established using the Megamix beads (B525/40 vs. Violet SSC), revealing signals for entities with the “stable green” surface marker (SHAG-EEVs, left panel), but not so for the “cytoplasmic green” marker (G-EEVs, middle panel), unless these entities were stained in the DNA with the SYTO 13 dye (G-EEVs + SYTO 13, right panel). C and D BC CytoFLEX S analysis with B 525/40 (green) versus R 660/20 (red) settings of SHAG-EEVs. The green fluorescence is progressively lost with sample dilution. E) F/T/S treatment of SHAG EEVs abolishes the green signal, confirming its membrane localization
Figure Legend Snippet: Visualization of fluorescent EEVs by Flow Virometry analysis. A Physical, FSC vs. Violet SSC plot (left panel), and fluorescence, B525/40 (green) vs. Violet SSC plot (right panel), characterisation of Megamix beads (900 nm—red, 500 nm—blue, 300 nm—yellow and 100 nm—green dots) by BC CytoFLEX S analysis, setting the instrument for flow virometry. B CEFs were infected with either rMVA-77-SHAG or rMVA-77-G- M1-V5, and supernatants were harvested 30 h p.i. Supernatants were analysed with the settings established using the Megamix beads (B525/40 vs. Violet SSC), revealing signals for entities with the “stable green” surface marker (SHAG-EEVs, left panel), but not so for the “cytoplasmic green” marker (G-EEVs, middle panel), unless these entities were stained in the DNA with the SYTO 13 dye (G-EEVs + SYTO 13, right panel). C and D BC CytoFLEX S analysis with B 525/40 (green) versus R 660/20 (red) settings of SHAG-EEVs. The green fluorescence is progressively lost with sample dilution. E) F/T/S treatment of SHAG EEVs abolishes the green signal, confirming its membrane localization

Techniques Used: Fluorescence, Infection, Marker, Staining


Structured Review

Becton Dickinson megamix beads
Megamix Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix beads/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix beads - by Bioz Stars, 2024-02
86/100 stars

Images


Structured Review

Becton Dickinson megamix beads
Megamix Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix beads/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix beads - by Bioz Stars, 2024-02
86/100 stars

Images


Structured Review

Becton Dickinson megamix fsc plus beads
Megamix Fsc Plus Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix fsc plus beads/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix fsc plus beads - by Bioz Stars, 2024-02
86/100 stars

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Structured Review

Becton Dickinson megamix fsc plus beads
Megamix Fsc Plus Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix fsc plus beads/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix fsc plus beads - by Bioz Stars, 2024-02
86/100 stars

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Structured Review

Becton Dickinson megamix plus fsc beads
Megamix Plus Fsc Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megamix plus fsc beads/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
megamix plus fsc beads - by Bioz Stars, 2024-02
86/100 stars

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    Becton Dickinson megamix beads
    Megamix Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megamix beads/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    megamix beads - by Bioz Stars, 2024-02
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson megamix fsc plus beads
    Megamix Fsc Plus Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megamix fsc plus beads/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    megamix fsc plus beads - by Bioz Stars, 2024-02
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson megamix plus fsc beads
    Megamix Plus Fsc Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megamix plus fsc beads/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    megamix plus fsc beads - by Bioz Stars, 2024-02
    86/100 stars
      Buy from Supplier

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