Structured Review

GE Healthcare megabace 1000
 Detection of SNPWave products with a flanking sizing standard in three consecutive 35 min runs on the MegaBACE 1000. One hundred-plex ligation using 625 ng of genomic DNA of  Arabidopsis  sample Kashmir-2 (N1264), and SNPWave amplification using JOE-labeled
Megabace 1000, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megabace 1000/product/GE Healthcare
Average 95 stars, based on 43 article reviews
Price from $9.99 to $1999.99
megabace 1000 - by Bioz Stars, 2020-09
95/100 stars

Images

1) Product Images from "SNPWaveTM: a flexible multiplexed SNP genotyping technology"

Article Title: SNPWaveTM: a flexible multiplexed SNP genotyping technology

Journal: Nucleic Acids Research

doi: 10.1093/nar/gnh045

 Detection of SNPWave products with a flanking sizing standard in three consecutive 35 min runs on the MegaBACE 1000. One hundred-plex ligation using 625 ng of genomic DNA of  Arabidopsis  sample Kashmir-2 (N1264), and SNPWave amplification using JOE-labeled
Figure Legend Snippet: Detection of SNPWave products with a flanking sizing standard in three consecutive 35 min runs on the MegaBACE 1000. One hundred-plex ligation using 625 ng of genomic DNA of Arabidopsis sample Kashmir-2 (N1264), and SNPWave amplification using JOE-labeled

Techniques Used: Ligation, Amplification, Labeling

2) Product Images from "SNPWaveTM: a flexible multiplexed SNP genotyping technology"

Article Title: SNPWaveTM: a flexible multiplexed SNP genotyping technology

Journal: Nucleic Acids Research

doi: 10.1093/nar/gnh045

 Detection of SNPWave products with a flanking sizing standard in three consecutive 35 min runs on the MegaBACE 1000. One hundred-plex ligation using 625 ng of genomic DNA of  Arabidopsis  sample Kashmir-2 (N1264), and SNPWave amplification using JOE-labeled
Figure Legend Snippet: Detection of SNPWave products with a flanking sizing standard in three consecutive 35 min runs on the MegaBACE 1000. One hundred-plex ligation using 625 ng of genomic DNA of Arabidopsis sample Kashmir-2 (N1264), and SNPWave amplification using JOE-labeled

Techniques Used: Ligation, Amplification, Labeling

Related Articles

Radio Immunoprecipitation:

Article Title: Lactic acid bacterium, Lactobacillus paracasei KW3110, suppresses inflammatory stress-induced caspase-1 activation by promoting interleukin-10 production in mouse and human immune cells
Article Snippet: .. The cells were then primed with 10 μg/mL of LPS for 4 h, and stimulated with 2 mM ATP for 1 h. After stimulation, cells were lysed with radioimmunoprecipitation assay buffer and whole cell lysates were prepared and then boiled in sodium dodecyl sulfate (SDS) sample buffer for 5 min at 95 ˚C. ..

Modification:

Article Title: Substrate binding in vitro and kinetics of RsrI [N6-adenine] DNA methyltransferase
Article Snippet: .. PhastSystem 20% gels, buffer strips, silver staining reagents and the PhastSystem were also from Amersham Pharmacia Biotech. λ DNA and restriction, modification and PCR enzymes were purchased from either Gibco BRL, Promega, New England Biolabs or Stratagene. .. S -adenosylmethionine (AdoMet) and S -adenosylhomocysteine (AdoHcy) were purchased from Sigma and purified ( ).

Incubation:

Article Title: Muscleblind-like 1 interacts with RNA hairpins in splicing target and pathogenic RNAs
Article Snippet: .. After three washes (10 ml each) of buffer (WB) containing 25 mM Tris–Cl, pH 8.0, 300 mM NaCl, 5 mM β-mercaptoethanol, 0.1% Triton X-100, the glutathione-Sepharose beads were incubated with 4 ml WB containing 40 U of PreScission protease (Amersham) at 4°C overnight. .. Chemical and enzymatic analysis of RNA structures Transcription reactions were carried out in a 50 µl volume which contained 2 μg of each DNA template, 1 mM rNTPs, 3.3 mM guanosine, 60 U of ribonuclease inhibitor RNase Out (Invitrogen), 200 U of T7 RNA polymerase (Ambion, Austin, TX, USA), 10 mM DTT, 40 mM Tris-HCl (pH 7.9), 6 mM MgCl2 , 2 mM spermidine, 10 mM NaCl.

Polymerase Chain Reaction:

Article Title: Substrate binding in vitro and kinetics of RsrI [N6-adenine] DNA methyltransferase
Article Snippet: .. PhastSystem 20% gels, buffer strips, silver staining reagents and the PhastSystem were also from Amersham Pharmacia Biotech. λ DNA and restriction, modification and PCR enzymes were purchased from either Gibco BRL, Promega, New England Biolabs or Stratagene. .. S -adenosylmethionine (AdoMet) and S -adenosylhomocysteine (AdoHcy) were purchased from Sigma and purified ( ).

Western Blot:

Article Title: Muscleblind-like 1 interacts with RNA hairpins in splicing target and pathogenic RNAs
Article Snippet: .. After three washes (10 ml each) of buffer (WB) containing 25 mM Tris–Cl, pH 8.0, 300 mM NaCl, 5 mM β-mercaptoethanol, 0.1% Triton X-100, the glutathione-Sepharose beads were incubated with 4 ml WB containing 40 U of PreScission protease (Amersham) at 4°C overnight. .. Chemical and enzymatic analysis of RNA structures Transcription reactions were carried out in a 50 µl volume which contained 2 μg of each DNA template, 1 mM rNTPs, 3.3 mM guanosine, 60 U of ribonuclease inhibitor RNase Out (Invitrogen), 200 U of T7 RNA polymerase (Ambion, Austin, TX, USA), 10 mM DTT, 40 mM Tris-HCl (pH 7.9), 6 mM MgCl2 , 2 mM spermidine, 10 mM NaCl.

Silver Staining:

Article Title: Substrate binding in vitro and kinetics of RsrI [N6-adenine] DNA methyltransferase
Article Snippet: .. PhastSystem 20% gels, buffer strips, silver staining reagents and the PhastSystem were also from Amersham Pharmacia Biotech. λ DNA and restriction, modification and PCR enzymes were purchased from either Gibco BRL, Promega, New England Biolabs or Stratagene. .. S -adenosylmethionine (AdoMet) and S -adenosylhomocysteine (AdoHcy) were purchased from Sigma and purified ( ).

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    Image Search Results


    RSCA electropherograms . Examples of RSCA electropherograms from the MegaBace™ 1000 for one individual

    Journal: BMC Research Notes

    Article Title: Genotyping of black grouse MHC class II B using reference Strand-Mediated Conformational Analysis (RSCA)

    doi: 10.1186/1756-0500-4-183

    Figure Lengend Snippet: RSCA electropherograms . Examples of RSCA electropherograms from the MegaBace™ 1000 for one individual "Ref26" using all four FLRs, a) FLR-Tete1, b) FLR-Bobo1, c) FLR-Bobo2 and d) FLR-Bobo3. On the x-axis are the migration values in the non-denaturing gel, the y-axis reflects the height of the peaks. The red peaks are the size markers and the blue peaks are the RSCA-peaks. The left most of the blue peaks are the FLR-homoduplex peak. The other blue peaks are used in the RSCA-scoring.

    Article Snippet: After genotyping RSCA in MegaBACE™ 1000, we viewed our electropherograms in the software MegaBACE Fragment Profiler™ version 1.2 (GE Healthcare) and assigned possible alleles (see Figure and Table for an example) for each FLR using the RSCA library and scoring rules.

    Techniques: Migration