Structured Review

Millipore mefloquine hydrochloride mq
Sensitization to several quinoline antimalarials by PF3D7_0629500 expression and reversal with the T162E SNP. ( A ) Yeast trp1Δ cells transformed with pCM190 vector, either empty (ev) or expressing PF3D7-0629500 (Pf protein) or the same protein carrying the T162E SNP (Pf-T162E), were cultured with either 1 mM chloroquine (CQ), 1 mM amodiaquine (AQ), 25 μM <t>mefloquine</t> (MQ) or 3 mM quinine (QN). Mean data are shown from at least three independent experiments ± SEM. ( B ) RNA was extracted from wild type yeast transformed as in ( A ) and mRNA corresponding to the wild type PF3D7-0629500 ORF or SNP (T162E) version was analysed with qRT-PCR, performed in triplicate for each condition. The same amount of RNA extract was used for each reaction. There was not a significant difference (ns, according to Student’s t -test, two tailed) between the conditions either when compared by raw counts (as shown) or after normalization against ACT1 mRNA. au, arbitrary units.
Mefloquine Hydrochloride Mq, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mefloquine hydrochloride mq/product/Millipore
Average 94 stars, based on 5 article reviews
Price from $9.99 to $1999.99
mefloquine hydrochloride mq - by Bioz Stars, 2022-09
94/100 stars

Images

1) Product Images from "Heterologous Expression of a Novel Drug Transporter from the Malaria Parasite Alters Resistance to Quinoline Antimalarials"

Article Title: Heterologous Expression of a Novel Drug Transporter from the Malaria Parasite Alters Resistance to Quinoline Antimalarials

Journal: Scientific Reports

doi: 10.1038/s41598-018-20816-0

Sensitization to several quinoline antimalarials by PF3D7_0629500 expression and reversal with the T162E SNP. ( A ) Yeast trp1Δ cells transformed with pCM190 vector, either empty (ev) or expressing PF3D7-0629500 (Pf protein) or the same protein carrying the T162E SNP (Pf-T162E), were cultured with either 1 mM chloroquine (CQ), 1 mM amodiaquine (AQ), 25 μM mefloquine (MQ) or 3 mM quinine (QN). Mean data are shown from at least three independent experiments ± SEM. ( B ) RNA was extracted from wild type yeast transformed as in ( A ) and mRNA corresponding to the wild type PF3D7-0629500 ORF or SNP (T162E) version was analysed with qRT-PCR, performed in triplicate for each condition. The same amount of RNA extract was used for each reaction. There was not a significant difference (ns, according to Student’s t -test, two tailed) between the conditions either when compared by raw counts (as shown) or after normalization against ACT1 mRNA. au, arbitrary units.
Figure Legend Snippet: Sensitization to several quinoline antimalarials by PF3D7_0629500 expression and reversal with the T162E SNP. ( A ) Yeast trp1Δ cells transformed with pCM190 vector, either empty (ev) or expressing PF3D7-0629500 (Pf protein) or the same protein carrying the T162E SNP (Pf-T162E), were cultured with either 1 mM chloroquine (CQ), 1 mM amodiaquine (AQ), 25 μM mefloquine (MQ) or 3 mM quinine (QN). Mean data are shown from at least three independent experiments ± SEM. ( B ) RNA was extracted from wild type yeast transformed as in ( A ) and mRNA corresponding to the wild type PF3D7-0629500 ORF or SNP (T162E) version was analysed with qRT-PCR, performed in triplicate for each condition. The same amount of RNA extract was used for each reaction. There was not a significant difference (ns, according to Student’s t -test, two tailed) between the conditions either when compared by raw counts (as shown) or after normalization against ACT1 mRNA. au, arbitrary units.

Techniques Used: Expressing, Transformation Assay, Plasmid Preparation, Cell Culture, Quantitative RT-PCR, Two Tailed Test

2) Product Images from "Heterologous Expression of a Novel Drug Transporter from the Malaria Parasite Alters Resistance to Quinoline Antimalarials"

Article Title: Heterologous Expression of a Novel Drug Transporter from the Malaria Parasite Alters Resistance to Quinoline Antimalarials

Journal: Scientific Reports

doi: 10.1038/s41598-018-20816-0

Sensitization to several quinoline antimalarials by PF3D7_0629500 expression and reversal with the T162E SNP. ( A ) Yeast trp1Δ cells transformed with pCM190 vector, either empty (ev) or expressing PF3D7-0629500 (Pf protein) or the same protein carrying the T162E SNP (Pf-T162E), were cultured with either 1 mM chloroquine (CQ), 1 mM amodiaquine (AQ), 25 μM mefloquine (MQ) or 3 mM quinine (QN). Mean data are shown from at least three independent experiments ± SEM. ( B ) RNA was extracted from wild type yeast transformed as in ( A ) and mRNA corresponding to the wild type PF3D7-0629500 ORF or SNP (T162E) version was analysed with qRT-PCR, performed in triplicate for each condition. The same amount of RNA extract was used for each reaction. There was not a significant difference (ns, according to Student’s t -test, two tailed) between the conditions either when compared by raw counts (as shown) or after normalization against ACT1 mRNA. au, arbitrary units.
Figure Legend Snippet: Sensitization to several quinoline antimalarials by PF3D7_0629500 expression and reversal with the T162E SNP. ( A ) Yeast trp1Δ cells transformed with pCM190 vector, either empty (ev) or expressing PF3D7-0629500 (Pf protein) or the same protein carrying the T162E SNP (Pf-T162E), were cultured with either 1 mM chloroquine (CQ), 1 mM amodiaquine (AQ), 25 μM mefloquine (MQ) or 3 mM quinine (QN). Mean data are shown from at least three independent experiments ± SEM. ( B ) RNA was extracted from wild type yeast transformed as in ( A ) and mRNA corresponding to the wild type PF3D7-0629500 ORF or SNP (T162E) version was analysed with qRT-PCR, performed in triplicate for each condition. The same amount of RNA extract was used for each reaction. There was not a significant difference (ns, according to Student’s t -test, two tailed) between the conditions either when compared by raw counts (as shown) or after normalization against ACT1 mRNA. au, arbitrary units.

Techniques Used: Expressing, Transformation Assay, Plasmid Preparation, Cell Culture, Quantitative RT-PCR, Two Tailed Test

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Millipore mefloquine hydrochloride
    (A) An example of paradoxical increase in signal with very high concentrations of <t>mefloquine.</t> (B) Paradoxical increased apparent growth at high drug concentrations for the seven drugs with flow cytometry (FCM) and DELI methods (see Materials and Methods for the definition and calculation of paradoxical growth).
    Mefloquine Hydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mefloquine hydrochloride/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mefloquine hydrochloride - by Bioz Stars, 2022-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    (A) An example of paradoxical increase in signal with very high concentrations of mefloquine. (B) Paradoxical increased apparent growth at high drug concentrations for the seven drugs with flow cytometry (FCM) and DELI methods (see Materials and Methods for the definition and calculation of paradoxical growth).

    Journal: Journal of Clinical Microbiology

    Article Title: Comparison between Flow Cytometry, Microscopy, and Lactate Dehydrogenase-Based Enzyme-Linked Immunosorbent Assay for Plasmodium falciparum Drug Susceptibility Testing under Field Conditions

    doi: 10.1128/JCM.01226-15

    Figure Lengend Snippet: (A) An example of paradoxical increase in signal with very high concentrations of mefloquine. (B) Paradoxical increased apparent growth at high drug concentrations for the seven drugs with flow cytometry (FCM) and DELI methods (see Materials and Methods for the definition and calculation of paradoxical growth).

    Article Snippet: These assays used artesunate (AS; molecular weight [M w ], 384.4 g/mol; Holly Pharmaceuticals Co. Ltd.), dihydroartemisinin (DHA; M w , 284.94; Fluka), lumefantrine (LUM; M w , 528.94; Sigma-Aldrich), mefloquine hydrochloride (MQ; M w , 414.77; Sigma-Aldrich), piperaquine tetraphosphate (PIP; M w , 999.56; Artekin Holley Pharmaceutical Co), chloroquine diphosphate (CQ; M w , 515.9; Sigma-Aldrich), and quinine hydrochloride (QN; M w , 396.9; Sigma-Aldrich).

    Techniques: Flow Cytometry, Cytometry

    Sensitization to several quinoline antimalarials by PF3D7_0629500 expression and reversal with the T162E SNP. ( A ) Yeast trp1Δ cells transformed with pCM190 vector, either empty (ev) or expressing PF3D7-0629500 (Pf protein) or the same protein carrying the T162E SNP (Pf-T162E), were cultured with either 1 mM chloroquine (CQ), 1 mM amodiaquine (AQ), 25 μM mefloquine (MQ) or 3 mM quinine (QN). Mean data are shown from at least three independent experiments ± SEM. ( B ) RNA was extracted from wild type yeast transformed as in ( A ) and mRNA corresponding to the wild type PF3D7-0629500 ORF or SNP (T162E) version was analysed with qRT-PCR, performed in triplicate for each condition. The same amount of RNA extract was used for each reaction. There was not a significant difference (ns, according to Student’s t -test, two tailed) between the conditions either when compared by raw counts (as shown) or after normalization against ACT1 mRNA. au, arbitrary units.

    Journal: Scientific Reports

    Article Title: Heterologous Expression of a Novel Drug Transporter from the Malaria Parasite Alters Resistance to Quinoline Antimalarials

    doi: 10.1038/s41598-018-20816-0

    Figure Lengend Snippet: Sensitization to several quinoline antimalarials by PF3D7_0629500 expression and reversal with the T162E SNP. ( A ) Yeast trp1Δ cells transformed with pCM190 vector, either empty (ev) or expressing PF3D7-0629500 (Pf protein) or the same protein carrying the T162E SNP (Pf-T162E), were cultured with either 1 mM chloroquine (CQ), 1 mM amodiaquine (AQ), 25 μM mefloquine (MQ) or 3 mM quinine (QN). Mean data are shown from at least three independent experiments ± SEM. ( B ) RNA was extracted from wild type yeast transformed as in ( A ) and mRNA corresponding to the wild type PF3D7-0629500 ORF or SNP (T162E) version was analysed with qRT-PCR, performed in triplicate for each condition. The same amount of RNA extract was used for each reaction. There was not a significant difference (ns, according to Student’s t -test, two tailed) between the conditions either when compared by raw counts (as shown) or after normalization against ACT1 mRNA. au, arbitrary units.

    Article Snippet: Antimalarial drugs used were amodiaquine dihydrochloride dihydrate (AMQ), chloroquine diphosphate salt (CQ), mefloquine hydrochloride (MQ) and quinine dihydrochloride (QN) (Sigma).

    Techniques: Expressing, Transformation Assay, Plasmid Preparation, Cell Culture, Quantitative RT-PCR, Two Tailed Test

    Manhattan plots of whole genome association tests. X-axis is Chromosomes 1 to 14 in alternating colors; Y-axis is the −log10 p-value from a Wilcoxon test; points in blue indicate P-values less than 0.0007 (above horizontal dashed line); DHA dihydroartemisinin (Fig. 2A), LM lumefantrine (Fig. 2B), PPQ piperaquine (Fig. 2C), CQ chloroquine (Fig. 2D) , PM pyrimethamine (Fig. 2E), MQ mefloquine (Fig. 2F), QN quinine (Fig. 2G), DEAQ desethylamodiaquine (Fig. 2H).

    Journal: Scientific Reports

    Article Title: Genome-wide screen identifies new candidate genes associated with artemisinin susceptibility in Plasmodium falciparum in Kenya

    doi: 10.1038/srep03318

    Figure Lengend Snippet: Manhattan plots of whole genome association tests. X-axis is Chromosomes 1 to 14 in alternating colors; Y-axis is the −log10 p-value from a Wilcoxon test; points in blue indicate P-values less than 0.0007 (above horizontal dashed line); DHA dihydroartemisinin (Fig. 2A), LM lumefantrine (Fig. 2B), PPQ piperaquine (Fig. 2C), CQ chloroquine (Fig. 2D) , PM pyrimethamine (Fig. 2E), MQ mefloquine (Fig. 2F), QN quinine (Fig. 2G), DEAQ desethylamodiaquine (Fig. 2H).

    Article Snippet: Growth inhibition of parasite cultures at 0.5% packed cell volume and 0.1% parasitemia was determined on 96-well plates by exposure to serial dilutions of dihydroartemisinin (DHA, Sigma), lumefantrine (LM, Novartis), piperaquine (PPQ, SigmaTau), chloroquine (CQ, Sigma), pyrimethamine (PM, Sigma), mefloquine (MQ, Sigma), N-desethylamodiaquine (DEAQ, Sigma) and quinine (QN, Sigma).

    Techniques:

    Half-maximal inhibitory concentrations (IC 50 ) for drug assays (phenotypes). The diagonal is a histogram of the phenotypes, right diagonal is the Spearman’s correlation between assays; left diagonal is raw data and smoothed relationship using cubic splines; DHA dihydroartemisinin , LM lumefantrine , PPQ piperaquine , CQ chloroquine, PM pyrimethamine , MQ mefloquine , QN quinine , DEAQ desethylamodiaquine .

    Journal: Scientific Reports

    Article Title: Genome-wide screen identifies new candidate genes associated with artemisinin susceptibility in Plasmodium falciparum in Kenya

    doi: 10.1038/srep03318

    Figure Lengend Snippet: Half-maximal inhibitory concentrations (IC 50 ) for drug assays (phenotypes). The diagonal is a histogram of the phenotypes, right diagonal is the Spearman’s correlation between assays; left diagonal is raw data and smoothed relationship using cubic splines; DHA dihydroartemisinin , LM lumefantrine , PPQ piperaquine , CQ chloroquine, PM pyrimethamine , MQ mefloquine , QN quinine , DEAQ desethylamodiaquine .

    Article Snippet: Growth inhibition of parasite cultures at 0.5% packed cell volume and 0.1% parasitemia was determined on 96-well plates by exposure to serial dilutions of dihydroartemisinin (DHA, Sigma), lumefantrine (LM, Novartis), piperaquine (PPQ, SigmaTau), chloroquine (CQ, Sigma), pyrimethamine (PM, Sigma), mefloquine (MQ, Sigma), N-desethylamodiaquine (DEAQ, Sigma) and quinine (QN, Sigma).

    Techniques:

    Response to P. chabaudi infection in drug-cured Bcl6, Blimp-1, and STAT3 TKO mice. TKO and WT animals were infected. Animals were given mefloquine (+MQ) starting on day 3 p.i. or left untreated (NTx). Splenocytes were harvested and analyzed by flow cytometry at day 7 p.i. (A) Contour plots show expression of IFN-γ and IL-21 in Teff. Bar graphs show average of fold change (%TKO/%WT) in a log2 scale of percentages of IFN-γ + IL-21 − and IFN-γ + IL-21 − Teff from NTx (black bars) and +MQ (white bars) from Bcl6 TKO (top), Blimp-1 TKO (middle), and STAT3 TKO (bottom). Intracellular cytokine staining in STAT3 TKO was the only one done with commercially prepared secretion inhibitor. (B) Contour plots show expression of PD-1 and CXCR5 in Teff from STAT3 TKO and WT mice. Bar graphs show percentages. Data representative of 2 experiments with 3 mice/group. † p

    Journal: bioRxiv

    Article Title: Increased Th1 bias in memory T cells corresponds with protection from reinfection in Plasmodium infection, and is regulated by T cell-intrinsic STAT3

    doi: 10.1101/724963

    Figure Lengend Snippet: Response to P. chabaudi infection in drug-cured Bcl6, Blimp-1, and STAT3 TKO mice. TKO and WT animals were infected. Animals were given mefloquine (+MQ) starting on day 3 p.i. or left untreated (NTx). Splenocytes were harvested and analyzed by flow cytometry at day 7 p.i. (A) Contour plots show expression of IFN-γ and IL-21 in Teff. Bar graphs show average of fold change (%TKO/%WT) in a log2 scale of percentages of IFN-γ + IL-21 − and IFN-γ + IL-21 − Teff from NTx (black bars) and +MQ (white bars) from Bcl6 TKO (top), Blimp-1 TKO (middle), and STAT3 TKO (bottom). Intracellular cytokine staining in STAT3 TKO was the only one done with commercially prepared secretion inhibitor. (B) Contour plots show expression of PD-1 and CXCR5 in Teff from STAT3 TKO and WT mice. Bar graphs show percentages. Data representative of 2 experiments with 3 mice/group. † p

    Article Snippet: In some experiments, mice were treated with mefloquine hydrochloride (MQ, 4mg/kg body weight, Sigma, St. Louis, MO) by oral gavage daily five times or until the mice were euthanized.

    Techniques: Infection, Mouse Assay, Flow Cytometry, Expressing, Staining

    Drug-cured mice have fewer IFN-γ + IL-21 + CXCR5 + hybrid Th1/Tfh, and more IFN-γ + IL-21 − CXCR5 − Th1-like cells. C57BL/6J mice were infected, and one group was treated with mefloquine (MQ) starting day 3 and splenocytes were analyzed at day 7 p.i. (A) Parasitemia curve from not-treated (NTx, black filled circles) and treated (+MQ, open circles) groups. Arrows depict treatment days. (B) Bar graph shows CD4 Teff numbers in NTx (black) and +MQ (white) at day 7 p.i. (C) Contour plots show expression of PD-1 and CXCR5 and (D) IFN-γ and IL-21 in Teff. Bar graphs show percentages and numbers of Teff subsets. (E) Histogram shows CXCR5 expression in IFN-γ + IL-21 − Teff at day 7 p.i. Bar graph shows CXCR5 MFI (Mean Fluorescence Intensity) fold change over isotype control. (F) Boolean gating analysis of all possible combinations of CXCR5, IFN-γ and IL-21 expression by Teff. (G) Contour plots show expression of CD38 and GL-7 in B cells (B220 hi MHC-II hi ). Bar graph shows numbers of GC B cells. Data representative of 3 experiments with 3-4 mice/group.

    Journal: bioRxiv

    Article Title: Increased Th1 bias in memory T cells corresponds with protection from reinfection in Plasmodium infection, and is regulated by T cell-intrinsic STAT3

    doi: 10.1101/724963

    Figure Lengend Snippet: Drug-cured mice have fewer IFN-γ + IL-21 + CXCR5 + hybrid Th1/Tfh, and more IFN-γ + IL-21 − CXCR5 − Th1-like cells. C57BL/6J mice were infected, and one group was treated with mefloquine (MQ) starting day 3 and splenocytes were analyzed at day 7 p.i. (A) Parasitemia curve from not-treated (NTx, black filled circles) and treated (+MQ, open circles) groups. Arrows depict treatment days. (B) Bar graph shows CD4 Teff numbers in NTx (black) and +MQ (white) at day 7 p.i. (C) Contour plots show expression of PD-1 and CXCR5 and (D) IFN-γ and IL-21 in Teff. Bar graphs show percentages and numbers of Teff subsets. (E) Histogram shows CXCR5 expression in IFN-γ + IL-21 − Teff at day 7 p.i. Bar graph shows CXCR5 MFI (Mean Fluorescence Intensity) fold change over isotype control. (F) Boolean gating analysis of all possible combinations of CXCR5, IFN-γ and IL-21 expression by Teff. (G) Contour plots show expression of CD38 and GL-7 in B cells (B220 hi MHC-II hi ). Bar graph shows numbers of GC B cells. Data representative of 3 experiments with 3-4 mice/group.

    Article Snippet: In some experiments, mice were treated with mefloquine hydrochloride (MQ, 4mg/kg body weight, Sigma, St. Louis, MO) by oral gavage daily five times or until the mice were euthanized.

    Techniques: Mouse Assay, Infection, Expressing, Fluorescence

    Day 5 mefloquine treatment has no effect on hybrid Th1/Tfh cell differentiation. C57BL/6J mice were infected, and one group was treated with mefloquine starting day 5 and splenocytes were analyzed at day 7 p.i. ( A ) Parasitemia on day 7 p.i. from untreated (NTx, black filled circles) and treated (+MQ, open circles) groups. Contour plots show expression of B ) PD-1 and CXCR5, and C) IFN-γ and IL-21 in Teff. Bar graphs show fraction of Teff and numbers of subsets. Data representative of 2 experiments with 3 mice/group.

    Journal: bioRxiv

    Article Title: Increased Th1 bias in memory T cells corresponds with protection from reinfection in Plasmodium infection, and is regulated by T cell-intrinsic STAT3

    doi: 10.1101/724963

    Figure Lengend Snippet: Day 5 mefloquine treatment has no effect on hybrid Th1/Tfh cell differentiation. C57BL/6J mice were infected, and one group was treated with mefloquine starting day 5 and splenocytes were analyzed at day 7 p.i. ( A ) Parasitemia on day 7 p.i. from untreated (NTx, black filled circles) and treated (+MQ, open circles) groups. Contour plots show expression of B ) PD-1 and CXCR5, and C) IFN-γ and IL-21 in Teff. Bar graphs show fraction of Teff and numbers of subsets. Data representative of 2 experiments with 3 mice/group.

    Article Snippet: In some experiments, mice were treated with mefloquine hydrochloride (MQ, 4mg/kg body weight, Sigma, St. Louis, MO) by oral gavage daily five times or until the mice were euthanized.

    Techniques: Cell Differentiation, Mouse Assay, Infection, Expressing