mec broth  (Millipore)


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  • 93
    Name:
    mEC Broth
    Description:

    Catalog Number:
    71882
    Price:
    None
    Applications:
    For the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods. The medium is used for the isolation and identification of E. coli 0157:H7 from meat acc. the USDA-FSIS method.
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    Structured Review

    Millipore mec broth

    https://www.bioz.com/result/mec broth/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mec broth - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Immunomagnetic Separation:

    Article Title: Reduction of Escherichia coli O157:H7 Shedding in Cattle by Addition of Chitosan Microparticles to Feed ▿
    Article Snippet: Enrichment combined with immunomagnetic separation (IMS) was used for presence or absence determinations on samples that did not yield E. coli O157 by direct plating. .. Samples were enriched in mEC broth supplemented with novobiocin (20 μg/ml; Sigma) for 18 to 24 h at 37°C with shaking, and E. coli O157:H7 was detected by using immunomagnetic anti-O157 beads as described by the manufacturer (Dynal, Carlsbad, CA).

    Modification:

    Article Title: Reduction of Escherichia coli O157:H7 Shedding in Cattle by Addition of Chitosan Microparticles to Feed ▿
    Article Snippet: Fecal samples were resuspended in (1:10-diluted) modified EC (mEC) broth (Difco), and swabs were suspended in 3 ml of mEC broth. .. Samples were enriched in mEC broth supplemented with novobiocin (20 μg/ml; Sigma) for 18 to 24 h at 37°C with shaking, and E. coli O157:H7 was detected by using immunomagnetic anti-O157 beads as described by the manufacturer (Dynal, Carlsbad, CA).

    Incubation:

    Article Title: Reduction of Escherichia coli O157:H7 Shedding in Cattle by Addition of Chitosan Microparticles to Feed ▿
    Article Snippet: Plates were incubated at 42°C for 18 to 24 h, and typical O157 colonies (i.e., sorbitol-negative colonies) that were O157 agglutination positive were enumerated. .. Samples were enriched in mEC broth supplemented with novobiocin (20 μg/ml; Sigma) for 18 to 24 h at 37°C with shaking, and E. coli O157:H7 was detected by using immunomagnetic anti-O157 beads as described by the manufacturer (Dynal, Carlsbad, CA).

    Agglutination:

    Article Title: Reduction of Escherichia coli O157:H7 Shedding in Cattle by Addition of Chitosan Microparticles to Feed ▿
    Article Snippet: Plates were incubated at 42°C for 18 to 24 h, and typical O157 colonies (i.e., sorbitol-negative colonies) that were O157 agglutination positive were enumerated. .. Samples were enriched in mEC broth supplemented with novobiocin (20 μg/ml; Sigma) for 18 to 24 h at 37°C with shaking, and E. coli O157:H7 was detected by using immunomagnetic anti-O157 beads as described by the manufacturer (Dynal, Carlsbad, CA).

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  • 88
    Millipore 5 mec
    Normal urotheliun. ( A ) Mouse IgG control; ( B ) 5-Methylocytosine <t>(5-MeC)</t> immunohistochemistry; ( C ) DNA (cytosine-5)-methyltransferase 1 (DNMT1) immunohistochemistry. High levels of both 5-MeC and DNMT1 present in the normal urothelium. Bar = 200 µm.
    5 Mec, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 mec/product/Millipore
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    5 mec - by Bioz Stars, 2020-04
    88/100 stars
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    86
    Millipore hesc mecs
    Identification of transplanted cells. ( A ) <t>hESC-MECs</t> and HeLa cells with mouse stroma adjacent to microcarriers (mc); hematoxylin staining. ( B ) G-6-P shown by enzyme histochemistry in transplanted hESC-MECs (arrows, brown cytoplasm). G-6-P is not expressed
    Hesc Mecs, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hesc mecs/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hesc mecs - by Bioz Stars, 2020-04
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    Image Search Results


    Normal urotheliun. ( A ) Mouse IgG control; ( B ) 5-Methylocytosine (5-MeC) immunohistochemistry; ( C ) DNA (cytosine-5)-methyltransferase 1 (DNMT1) immunohistochemistry. High levels of both 5-MeC and DNMT1 present in the normal urothelium. Bar = 200 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Reduced 5-Methylcytosine Level as a Potential Progression Predictor in Patients with T1 or Non-Invasive Urothelial Carcinoma

    doi: 10.3390/ijms16010677

    Figure Lengend Snippet: Normal urotheliun. ( A ) Mouse IgG control; ( B ) 5-Methylocytosine (5-MeC) immunohistochemistry; ( C ) DNA (cytosine-5)-methyltransferase 1 (DNMT1) immunohistochemistry. High levels of both 5-MeC and DNMT1 present in the normal urothelium. Bar = 200 µm.

    Article Snippet: UC values greater than 106 or 181 of the H-score were considered high 5-MeC or DNMT1 cancers; conversely, UC values less than or equal to 106 or 181 were considered low 5-MeC or DNMT1 cancers ( ).

    Techniques: Immunohistochemistry

    Representative photography of 5-MeC and DNMT1 immunohistochemistry. The 5-MeC photography in A – E ; The DNMT1 photos in F – J . ( A , F ) High-grade urothelial carcinoma showing high levels of both 5-MeC and DNMT1; ( B , G ) Low-grade urothelial carcinoma showing high levels of both 5-MeC and DNMT1; ( C , H ) High-grade urothelial carcinoma with glandular differentiation, showing high 5-MeC but low DNMT1 levels. ( D , I ) High-grade urothelial carcinoma with squamous differentiation, showing low levels of both 5-MeC and DNMT1; ( E , J ) High-grade urothelial carcinoma showing low levels of both 5-MeC and DNMT1. Bar = 200 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Reduced 5-Methylcytosine Level as a Potential Progression Predictor in Patients with T1 or Non-Invasive Urothelial Carcinoma

    doi: 10.3390/ijms16010677

    Figure Lengend Snippet: Representative photography of 5-MeC and DNMT1 immunohistochemistry. The 5-MeC photography in A – E ; The DNMT1 photos in F – J . ( A , F ) High-grade urothelial carcinoma showing high levels of both 5-MeC and DNMT1; ( B , G ) Low-grade urothelial carcinoma showing high levels of both 5-MeC and DNMT1; ( C , H ) High-grade urothelial carcinoma with glandular differentiation, showing high 5-MeC but low DNMT1 levels. ( D , I ) High-grade urothelial carcinoma with squamous differentiation, showing low levels of both 5-MeC and DNMT1; ( E , J ) High-grade urothelial carcinoma showing low levels of both 5-MeC and DNMT1. Bar = 200 µm.

    Article Snippet: UC values greater than 106 or 181 of the H-score were considered high 5-MeC or DNMT1 cancers; conversely, UC values less than or equal to 106 or 181 were considered low 5-MeC or DNMT1 cancers ( ).

    Techniques: Immunohistochemistry

    Identification of transplanted cells. ( A ) hESC-MECs and HeLa cells with mouse stroma adjacent to microcarriers (mc); hematoxylin staining. ( B ) G-6-P shown by enzyme histochemistry in transplanted hESC-MECs (arrows, brown cytoplasm). G-6-P is not expressed

    Journal: Journal of Cell Science

    Article Title: Spontaneous origin from human embryonic stem cells of liver cells displaying conjoint meso-endodermal phenotype with hepatic functions

    doi: 10.1242/jcs.095372

    Figure Lengend Snippet: Identification of transplanted cells. ( A ) hESC-MECs and HeLa cells with mouse stroma adjacent to microcarriers (mc); hematoxylin staining. ( B ) G-6-P shown by enzyme histochemistry in transplanted hESC-MECs (arrows, brown cytoplasm). G-6-P is not expressed

    Article Snippet: For conditioned medium, hESC-MECs were cultured for 24 hours in complete medium followed by in DMEM for 24 hours, which was harvested and passed through a 0.22 μm filter (Millipore, Billerica, MA).

    Techniques: Staining

    Liver regeneration after transplantation of hESC-MECs. ( A ) Gross appearance of abdominal organs in sham-treated and hESC-MEC-treated mice. Note extensive liver edema and necrosis in sham-treated mouse after 2 weeks compared with normal appearing liver

    Journal: Journal of Cell Science

    Article Title: Spontaneous origin from human embryonic stem cells of liver cells displaying conjoint meso-endodermal phenotype with hepatic functions

    doi: 10.1242/jcs.095372

    Figure Lengend Snippet: Liver regeneration after transplantation of hESC-MECs. ( A ) Gross appearance of abdominal organs in sham-treated and hESC-MEC-treated mice. Note extensive liver edema and necrosis in sham-treated mouse after 2 weeks compared with normal appearing liver

    Article Snippet: For conditioned medium, hESC-MECs were cultured for 24 hours in complete medium followed by in DMEM for 24 hours, which was harvested and passed through a 0.22 μm filter (Millipore, Billerica, MA).

    Techniques: Transplantation Assay, Mouse Assay

    Characterization of hESC-MECs. ( A–C ) Immunostaining for pluripotency markers Oct4, SSEA4 and TRA-1-60 in undifferentiated hESCs (A), hESC-MECs (B) and FH-Ep-P3 cells (C). ( D ) RT-PCR gene expression analysis in hESCs, hESC-MECs, FH-Ep-P3 and FH-Ep-PP

    Journal: Journal of Cell Science

    Article Title: Spontaneous origin from human embryonic stem cells of liver cells displaying conjoint meso-endodermal phenotype with hepatic functions

    doi: 10.1242/jcs.095372

    Figure Lengend Snippet: Characterization of hESC-MECs. ( A–C ) Immunostaining for pluripotency markers Oct4, SSEA4 and TRA-1-60 in undifferentiated hESCs (A), hESC-MECs (B) and FH-Ep-P3 cells (C). ( D ) RT-PCR gene expression analysis in hESCs, hESC-MECs, FH-Ep-P3 and FH-Ep-PP

    Article Snippet: For conditioned medium, hESC-MECs were cultured for 24 hours in complete medium followed by in DMEM for 24 hours, which was harvested and passed through a 0.22 μm filter (Millipore, Billerica, MA).

    Techniques: Immunostaining, Reverse Transcription Polymerase Chain Reaction, Expressing

    Outcomes in mice with severe ALF and effects of conditioned medium. ( A ) Survival in mice given Rif, Phen and 160 mg/kg MCT followed by either sham treatment or transplantation of hESC-MECs ( n =8 each). Seven of eight sham-treated mice died 3 days after

    Journal: Journal of Cell Science

    Article Title: Spontaneous origin from human embryonic stem cells of liver cells displaying conjoint meso-endodermal phenotype with hepatic functions

    doi: 10.1242/jcs.095372

    Figure Lengend Snippet: Outcomes in mice with severe ALF and effects of conditioned medium. ( A ) Survival in mice given Rif, Phen and 160 mg/kg MCT followed by either sham treatment or transplantation of hESC-MECs ( n =8 each). Seven of eight sham-treated mice died 3 days after

    Article Snippet: For conditioned medium, hESC-MECs were cultured for 24 hours in complete medium followed by in DMEM for 24 hours, which was harvested and passed through a 0.22 μm filter (Millipore, Billerica, MA).

    Techniques: Mouse Assay, Transplantation Assay

    Morphological properties of cells. ( A–D ) Phase-contrast micrographs showing (A) undifferentiated hESCs, (B) hESC-MECs, (C) Ep-CAM-positive primary fetal liver stem cells (FH-Ep-PP) and (D) FH-Ep-P3 fetal liver cells after three passages in culture.

    Journal: Journal of Cell Science

    Article Title: Spontaneous origin from human embryonic stem cells of liver cells displaying conjoint meso-endodermal phenotype with hepatic functions

    doi: 10.1242/jcs.095372

    Figure Lengend Snippet: Morphological properties of cells. ( A–D ) Phase-contrast micrographs showing (A) undifferentiated hESCs, (B) hESC-MECs, (C) Ep-CAM-positive primary fetal liver stem cells (FH-Ep-PP) and (D) FH-Ep-P3 fetal liver cells after three passages in culture.

    Article Snippet: For conditioned medium, hESC-MECs were cultured for 24 hours in complete medium followed by in DMEM for 24 hours, which was harvested and passed through a 0.22 μm filter (Millipore, Billerica, MA).

    Techniques: Chick Chorioallantoic Membrane Assay

    Mesenchymal and hepatic properties in hESC-MECs. ( A ) Cytostaining in hESCs, hESC-MECs and FH-Ep-P3 cells for hepatobiliary markers glycogen, G-6-P and GGT, and the mesenchymal marker vimentin. hESCs stained for glycogen. hESC-MECs and FH-Ep-P3 cells display

    Journal: Journal of Cell Science

    Article Title: Spontaneous origin from human embryonic stem cells of liver cells displaying conjoint meso-endodermal phenotype with hepatic functions

    doi: 10.1242/jcs.095372

    Figure Lengend Snippet: Mesenchymal and hepatic properties in hESC-MECs. ( A ) Cytostaining in hESCs, hESC-MECs and FH-Ep-P3 cells for hepatobiliary markers glycogen, G-6-P and GGT, and the mesenchymal marker vimentin. hESCs stained for glycogen. hESC-MECs and FH-Ep-P3 cells display

    Article Snippet: For conditioned medium, hESC-MECs were cultured for 24 hours in complete medium followed by in DMEM for 24 hours, which was harvested and passed through a 0.22 μm filter (Millipore, Billerica, MA).

    Techniques: Marker, Staining

    Hepatic functions in transplanted hESC-MECs. ( A–C ) hESC-MECs transduced with lentiviral vectors containing the Alb promoter to drive GFP expression. Cells were transplanted with microcarriers (mc) into peritoneal cavity in NOD/SCID mice. Two weeks

    Journal: Journal of Cell Science

    Article Title: Spontaneous origin from human embryonic stem cells of liver cells displaying conjoint meso-endodermal phenotype with hepatic functions

    doi: 10.1242/jcs.095372

    Figure Lengend Snippet: Hepatic functions in transplanted hESC-MECs. ( A–C ) hESC-MECs transduced with lentiviral vectors containing the Alb promoter to drive GFP expression. Cells were transplanted with microcarriers (mc) into peritoneal cavity in NOD/SCID mice. Two weeks

    Article Snippet: For conditioned medium, hESC-MECs were cultured for 24 hours in complete medium followed by in DMEM for 24 hours, which was harvested and passed through a 0.22 μm filter (Millipore, Billerica, MA).

    Techniques: Transduction, Expressing, Mouse Assay