Journal: Vaccines

Article Title: Development of a Novel Live Attenuated Influenza A Virus Vaccine Encoding the IgA-Inducing Protein

doi: 10.3390/vaccines9070703

Figure Lengend Snippet: In vitro growth kinetics. ( A ) Alignment of the predicted IGIP in different mammalian species. The mature swine IGIP sequence used in this study is shown. ( B ) Schematic representation of the IGIP-H1 gene. The IGIP sequence and the components of the intergenic region are indicated. ( C ) Growth kinetics profiles of IGIP-H1att and H1att in MDCK and MDCK STAT1 KO cells. Experiments were performed two times independently, each time in triplicate. Titers were determined by RT-qPCR and expressed as log 10 TCID50 equivalents. Gray area represents the area below the level of detection of the assay. ( D ) IGIP-H1att virus was serially passaged five times in MDCK (E1C5) cells and SPF eggs (E5), and the HA was amplified by RT-PCR, showing that the IGIP-H1 rearrangement is stable. Abbreviations: FP, fusion peptide; TM, transmembrane domain; CT, c-terminal region; G4S, poly-glycine protein linker; Furin CS, furin cleavage site; Tav2A, Thosea assigna virus 2A protein sequence; GlucS, Signal peptide of Gaussia luciferase.

Article Snippet: MDCK STAT1 KO cells (CCL-34-VHG) were purchased from ATCC.

Techniques: In Vitro, Sequencing, Quantitative RT-PCR, Amplification, Reverse Transcription Polymerase Chain Reaction, Luciferase