mda mb 468  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC mda mb 468
    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , <t>MM468</t> wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Mda Mb 468, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda mb 468/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mda mb 468 - by Bioz Stars, 2024-03
    86/100 stars

    Images

    1) Product Images from "Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy"

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    Journal: Oncology Letters

    doi: 10.3892/ol.2024.14291

    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Figure Legend Snippet: (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.

    Techniques Used: Expressing, Microarray

    (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.
    Figure Legend Snippet: (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.

    Techniques Used: Real-time Polymerase Chain Reaction

    (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.
    Figure Legend Snippet: (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing, Incubation

    human bc cell lines mda mb 231  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC human bc cell lines mda mb 231
    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in <t>MM231</t> wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Human Bc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human bc cell lines mda mb 231/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human bc cell lines mda mb 231 - by Bioz Stars, 2024-03
    86/100 stars

    Images

    1) Product Images from "Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy"

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    Journal: Oncology Letters

    doi: 10.3892/ol.2024.14291

    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Figure Legend Snippet: (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.

    Techniques Used: Expressing, Microarray

    (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.
    Figure Legend Snippet: (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.

    Techniques Used: Real-time Polymerase Chain Reaction

    (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.
    Figure Legend Snippet: (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing, Incubation

    mda mb 468  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC mda mb 468
    Hypoxia suppresses PL2L60 protein expression in various types of cancer cells. (A and B) The effects of oxygen levels on PL2L60 expression in various types of cancer cell lines grown in normoxia (N; 21% O 2 ) or hypoxia (H; 1% O 2 ) for 24 h. The cells were harvested and measured for PL2L60 protein levels by western blotting. (A) Breast cancer cells including MDA-MB-231, <t>MDA-MB-468,</t> MCF-7, T-47D and SKBR3. (B) Cancer cell lines from various types of tissues including lung (A549), colon (HCT116), cervix (HeLa), prostate (PC3) and liver (HepG2). (C-F) Kinetics of hypoxia-induced decreasing PL2L60 expression in various types of cancer cell lines, including (C) MDA-MB-231, (D) MCF-7, (E) SKBR3 and (F) HeLa, which were cultured in hypoxia (H; 1% O 2 ) for 0, 12, 24 and 48 h. It should be noted that PL2L60 was consistently decreased at 24 h under hypoxia. PL2L60, Piwil2-like 60 kDa protein.
    Mda Mb 468, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda mb 468/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mda mb 468 - by Bioz Stars, 2024-03
    86/100 stars

    Images

    1) Product Images from "Selective degradation of PL2L60 by metabolic stresses‑induced autophagy suppresses multi‑cancer growth"

    Article Title: Selective degradation of PL2L60 by metabolic stresses‑induced autophagy suppresses multi‑cancer growth

    Journal: Oncology Reports

    doi: 10.3892/or.2024.8700

    Hypoxia suppresses PL2L60 protein expression in various types of cancer cells. (A and B) The effects of oxygen levels on PL2L60 expression in various types of cancer cell lines grown in normoxia (N; 21% O 2 ) or hypoxia (H; 1% O 2 ) for 24 h. The cells were harvested and measured for PL2L60 protein levels by western blotting. (A) Breast cancer cells including MDA-MB-231, MDA-MB-468, MCF-7, T-47D and SKBR3. (B) Cancer cell lines from various types of tissues including lung (A549), colon (HCT116), cervix (HeLa), prostate (PC3) and liver (HepG2). (C-F) Kinetics of hypoxia-induced decreasing PL2L60 expression in various types of cancer cell lines, including (C) MDA-MB-231, (D) MCF-7, (E) SKBR3 and (F) HeLa, which were cultured in hypoxia (H; 1% O 2 ) for 0, 12, 24 and 48 h. It should be noted that PL2L60 was consistently decreased at 24 h under hypoxia. PL2L60, Piwil2-like 60 kDa protein.
    Figure Legend Snippet: Hypoxia suppresses PL2L60 protein expression in various types of cancer cells. (A and B) The effects of oxygen levels on PL2L60 expression in various types of cancer cell lines grown in normoxia (N; 21% O 2 ) or hypoxia (H; 1% O 2 ) for 24 h. The cells were harvested and measured for PL2L60 protein levels by western blotting. (A) Breast cancer cells including MDA-MB-231, MDA-MB-468, MCF-7, T-47D and SKBR3. (B) Cancer cell lines from various types of tissues including lung (A549), colon (HCT116), cervix (HeLa), prostate (PC3) and liver (HepG2). (C-F) Kinetics of hypoxia-induced decreasing PL2L60 expression in various types of cancer cell lines, including (C) MDA-MB-231, (D) MCF-7, (E) SKBR3 and (F) HeLa, which were cultured in hypoxia (H; 1% O 2 ) for 0, 12, 24 and 48 h. It should be noted that PL2L60 was consistently decreased at 24 h under hypoxia. PL2L60, Piwil2-like 60 kDa protein.

    Techniques Used: Expressing, Western Blot, Cell Culture

    crl 1472 fbs 2 mda mb breast atcc adherent leibovitz s l 468 htb  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC crl 1472 fbs 2 mda mb breast atcc adherent leibovitz s l 468 htb
    Crl 1472 Fbs 2 Mda Mb Breast Atcc Adherent Leibovitz S L 468 Htb, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/crl 1472 fbs 2 mda mb breast atcc adherent leibovitz s l 468 htb/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    crl 1472 fbs 2 mda mb breast atcc adherent leibovitz s l 468 htb - by Bioz Stars, 2024-03
    86/100 stars

    Images

    mda mb 231  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC mda mb 231
    Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda mb 231/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mda mb 231 - by Bioz Stars, 2024-03
    86/100 stars

    Images

    fbs 2 mda mb breast atcc adherent leibovitz s l  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC fbs 2 mda mb breast atcc adherent leibovitz s l
    Fbs 2 Mda Mb Breast Atcc Adherent Leibovitz S L, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fbs 2 mda mb breast atcc adherent leibovitz s l/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fbs 2 mda mb breast atcc adherent leibovitz s l - by Bioz Stars, 2024-03
    86/100 stars

    Images

    bc cell line mda mb 231  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC bc cell line mda mb 231
    Bc Cell Line Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bc cell line mda mb 231/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bc cell line mda mb 231 - by Bioz Stars, 2024-03
    86/100 stars

    Images

    mda mb 231  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC mda mb 231
    Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda mb 231/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mda mb 231 - by Bioz Stars, 2024-03
    86/100 stars

    Images

    mda mb 231  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC mda mb 231
    SLC25A17 knockdown inhibited TNBC cell proliferation. A SLC25A17 mRNA and protein expression were higher in TNBC cell lines than in breast epithelial cell. B, C CCK8 and EdU assays were performed to evaluate the effect of SLC25A17 knockdown on proliferative <t>capacity</t> <t>in</t> <t>MDA-MB-231</t> and MDA-MB-468 cells. D The apoptosis level was assessed by flow cytometric analysis in MDA-MB-231 and MDA-MB-468 cells. E Colony formation assays were performed to evaluate the proliferative capacity in MDA-MB-231 and MDA-MB-468 cells. F The expression levels of apoptosis-related and cell cycle-related proteins were detected by western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
    Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda mb 231/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mda mb 231 - by Bioz Stars, 2024-03
    86/100 stars

    Images

    1) Product Images from "SLC25A17 inhibits autophagy to promote triple-negative breast cancer tumorigenesis by ROS-mediated JAK2/STAT3 signaling pathway"

    Article Title: SLC25A17 inhibits autophagy to promote triple-negative breast cancer tumorigenesis by ROS-mediated JAK2/STAT3 signaling pathway

    Journal: Cancer Cell International

    doi: 10.1186/s12935-024-03270-z

    SLC25A17 knockdown inhibited TNBC cell proliferation. A SLC25A17 mRNA and protein expression were higher in TNBC cell lines than in breast epithelial cell. B, C CCK8 and EdU assays were performed to evaluate the effect of SLC25A17 knockdown on proliferative capacity in MDA-MB-231 and MDA-MB-468 cells. D The apoptosis level was assessed by flow cytometric analysis in MDA-MB-231 and MDA-MB-468 cells. E Colony formation assays were performed to evaluate the proliferative capacity in MDA-MB-231 and MDA-MB-468 cells. F The expression levels of apoptosis-related and cell cycle-related proteins were detected by western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: SLC25A17 knockdown inhibited TNBC cell proliferation. A SLC25A17 mRNA and protein expression were higher in TNBC cell lines than in breast epithelial cell. B, C CCK8 and EdU assays were performed to evaluate the effect of SLC25A17 knockdown on proliferative capacity in MDA-MB-231 and MDA-MB-468 cells. D The apoptosis level was assessed by flow cytometric analysis in MDA-MB-231 and MDA-MB-468 cells. E Colony formation assays were performed to evaluate the proliferative capacity in MDA-MB-231 and MDA-MB-468 cells. F The expression levels of apoptosis-related and cell cycle-related proteins were detected by western blot. * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Expressing, Western Blot

    SLC25A17 knockdown inhibited migration, invasion, and EMT of TNBC cells. A Representative transwell migration and invasion images and statistics for the number of migrated and invaded TNBC cells with SLC25A17 knockdown. (scale bar: 100 μm). B Representative pictures and quantitative data of wound healing assay in MDA-MB-231 and MDA-MB-468 with SLC25A17 knockdown. C Immunofluorescence staining analysis of EMT markers of E-Cadherin and Vimentin (red), and the nuclei were stained with DAPI (blue) (scale bar: 100 μm). D Invasion-related proteins, E-Cadherin, Vimentin, MMP9 and MMP2 were detected by western blot analysis. * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: SLC25A17 knockdown inhibited migration, invasion, and EMT of TNBC cells. A Representative transwell migration and invasion images and statistics for the number of migrated and invaded TNBC cells with SLC25A17 knockdown. (scale bar: 100 μm). B Representative pictures and quantitative data of wound healing assay in MDA-MB-231 and MDA-MB-468 with SLC25A17 knockdown. C Immunofluorescence staining analysis of EMT markers of E-Cadherin and Vimentin (red), and the nuclei were stained with DAPI (blue) (scale bar: 100 μm). D Invasion-related proteins, E-Cadherin, Vimentin, MMP9 and MMP2 were detected by western blot analysis. * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Migration, Wound Healing Assay, Immunofluorescence, Staining, Western Blot

    SLC25A17 knockdown induces autophagy in TNBC cells. A Expression levels of P62, Beclin-1 and LC3-I, LC3-II in SLC25A17 knockdown or overexpression cells were detected by western blotting. B Representative LC3 immunofluorescence staining using an anti-LC3 antibody in SLC25A17 knockdown cells. (scale bar: 100 μm). C Representative images of MDC staining for autophagosomes in SLC25A17 knockdown cells. (scale bar: 100 μm). D Detection of autophagic flux by the mRFP-GFP-LC3 lentivirus in SLC25A17 knockdown MDA-MB-231 cells with confocal microscopy. (scale bar: 10 μm). E The number of autophagosomes was detected by electron microscopy in SLC25A17 knockdown MDA-MB-231 cells, and the autophagosomes were indicated by red arrows. (scale bar: 2 μm). F Western blot analysis of LC3-I to LC3-II conversion in SLC25A17 knockdown cells with or without chloroquine (CQ, 20 µM)
    Figure Legend Snippet: SLC25A17 knockdown induces autophagy in TNBC cells. A Expression levels of P62, Beclin-1 and LC3-I, LC3-II in SLC25A17 knockdown or overexpression cells were detected by western blotting. B Representative LC3 immunofluorescence staining using an anti-LC3 antibody in SLC25A17 knockdown cells. (scale bar: 100 μm). C Representative images of MDC staining for autophagosomes in SLC25A17 knockdown cells. (scale bar: 100 μm). D Detection of autophagic flux by the mRFP-GFP-LC3 lentivirus in SLC25A17 knockdown MDA-MB-231 cells with confocal microscopy. (scale bar: 10 μm). E The number of autophagosomes was detected by electron microscopy in SLC25A17 knockdown MDA-MB-231 cells, and the autophagosomes were indicated by red arrows. (scale bar: 2 μm). F Western blot analysis of LC3-I to LC3-II conversion in SLC25A17 knockdown cells with or without chloroquine (CQ, 20 µM)

    Techniques Used: Expressing, Over Expression, Western Blot, Immunofluorescence, Staining, Confocal Microscopy, Electron Microscopy

    SLC25A17 knockdown inhibited motility and proliferation mediated by autophagy. A, B Apoptosis analysis and colony formation in SLC25A17 knockdown MDA-MB-231 cells with or without 3-MA (5 mM). 3-MA: 3-methyladenine. C The protein levels of LC3-I, LC3-II, P62, cleaved caspase-3, cleaved-PARP, E-cadherin, Vimentin and MMP9 were detected by western blotting in MDA-MB-231 cells. D, E Representative transwell migration and invasion images and statistics for the number of migrated and invaded TNBC cells in SLC25A17 knockdown MDA-MB-231 cells with or without 3-MA (5 mM). * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: SLC25A17 knockdown inhibited motility and proliferation mediated by autophagy. A, B Apoptosis analysis and colony formation in SLC25A17 knockdown MDA-MB-231 cells with or without 3-MA (5 mM). 3-MA: 3-methyladenine. C The protein levels of LC3-I, LC3-II, P62, cleaved caspase-3, cleaved-PARP, E-cadherin, Vimentin and MMP9 were detected by western blotting in MDA-MB-231 cells. D, E Representative transwell migration and invasion images and statistics for the number of migrated and invaded TNBC cells in SLC25A17 knockdown MDA-MB-231 cells with or without 3-MA (5 mM). * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Western Blot, Migration

    ROS induced by SLC25A17 knockdown promoted apoptosis and autophagy in TNBC cells. A Flow cytometric analysis and quantification of ROS production in MDA-MB-231 and MDA-MB-468 cells. B The level of ROS was observed by fluorescence microscopy in SLC25A17 knockdown or overexpression MDA-MB-231 and MDA-MB-468 cells. (scale bar: 100 μm). C The protein levels of autophagy and apoptosis-related proteins was detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without NAC (5 mM). * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: ROS induced by SLC25A17 knockdown promoted apoptosis and autophagy in TNBC cells. A Flow cytometric analysis and quantification of ROS production in MDA-MB-231 and MDA-MB-468 cells. B The level of ROS was observed by fluorescence microscopy in SLC25A17 knockdown or overexpression MDA-MB-231 and MDA-MB-468 cells. (scale bar: 100 μm). C The protein levels of autophagy and apoptosis-related proteins was detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without NAC (5 mM). * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Fluorescence, Microscopy, Over Expression, Western Blot

    SLC25A17 knockdown inhibited JAK2/STAT3 pathway in TNBC cells. A The protein levels of phosphorylated STAT3 (p-STAT3) and phosphorylated JAK2 (p-JAK2) was detected by western blot in MDA-MB-231 and MDA-MB-468 cells. B The protein levels of p-STAT3 and its downstream protein Cyclin D and Bcl-2 were detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without NAC (5 mM). C The protein levels of autophagy and apoptosis-related proteins was detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without IL-6 (50 ng/mL). D Colony formation in SLC25A17 knockdown MDA-MB-231 cells with or without IL-6 (50 ng/mL). E Representative transwell invasion images and statistics for the number of invaded cells in SLC25A17 knockdown MDA-MB-231 cells with or without IL-6 (50 ng/mL). * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: SLC25A17 knockdown inhibited JAK2/STAT3 pathway in TNBC cells. A The protein levels of phosphorylated STAT3 (p-STAT3) and phosphorylated JAK2 (p-JAK2) was detected by western blot in MDA-MB-231 and MDA-MB-468 cells. B The protein levels of p-STAT3 and its downstream protein Cyclin D and Bcl-2 were detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without NAC (5 mM). C The protein levels of autophagy and apoptosis-related proteins was detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without IL-6 (50 ng/mL). D Colony formation in SLC25A17 knockdown MDA-MB-231 cells with or without IL-6 (50 ng/mL). E Representative transwell invasion images and statistics for the number of invaded cells in SLC25A17 knockdown MDA-MB-231 cells with or without IL-6 (50 ng/mL). * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Western Blot

    Knockdown of SLC25A17 inhibited the growth and metastasis of xenograft tumors in vivo. A Gross appearance of tumors from BALB/c nude mice injected with MDA-MB-231 cells with or without SLC25A17 knockdown. B The subcutaneous tumor volumes were tracked for 24 days. C Quantification of subcutaneous tumor weight of the nude mice in each group. D Representative IHC staining of p-STAT3, LC3, Ki-67 and cleaved-caspase3 in tumor tissues. (scale bar: 100 μm). E The protein level of LC3, Bcl-2, P62, Cyclin D, cleaved caspase 3 and p-STAT3 by western blot in xenograft tumors. F Proposed possible mechanism of SLC25A17 functions in TNBC. SLC25A17 inhibits apoptosis and autophagy by the regulation of ROS/JAK2/STAT3 signaling pathways. Image created using biorender.com. * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: Knockdown of SLC25A17 inhibited the growth and metastasis of xenograft tumors in vivo. A Gross appearance of tumors from BALB/c nude mice injected with MDA-MB-231 cells with or without SLC25A17 knockdown. B The subcutaneous tumor volumes were tracked for 24 days. C Quantification of subcutaneous tumor weight of the nude mice in each group. D Representative IHC staining of p-STAT3, LC3, Ki-67 and cleaved-caspase3 in tumor tissues. (scale bar: 100 μm). E The protein level of LC3, Bcl-2, P62, Cyclin D, cleaved caspase 3 and p-STAT3 by western blot in xenograft tumors. F Proposed possible mechanism of SLC25A17 functions in TNBC. SLC25A17 inhibits apoptosis and autophagy by the regulation of ROS/JAK2/STAT3 signaling pathways. Image created using biorender.com. * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: In Vivo, Injection, Immunohistochemistry, Western Blot

    mda mb 468  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC mda mb 468
    SLC25A17 knockdown inhibited TNBC cell proliferation. A SLC25A17 mRNA and protein expression were higher in TNBC cell lines than in breast epithelial cell. B, C CCK8 and EdU assays were performed to evaluate the effect of SLC25A17 knockdown on proliferative capacity in MDA-MB-231 and <t>MDA-MB-468</t> cells. D The apoptosis level was assessed by flow cytometric analysis in MDA-MB-231 and MDA-MB-468 cells. E Colony formation assays were performed to evaluate the proliferative capacity in MDA-MB-231 and MDA-MB-468 cells. F The expression levels of apoptosis-related and cell cycle-related proteins were detected by western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
    Mda Mb 468, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda mb 468/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mda mb 468 - by Bioz Stars, 2024-03
    86/100 stars

    Images

    1) Product Images from "SLC25A17 inhibits autophagy to promote triple-negative breast cancer tumorigenesis by ROS-mediated JAK2/STAT3 signaling pathway"

    Article Title: SLC25A17 inhibits autophagy to promote triple-negative breast cancer tumorigenesis by ROS-mediated JAK2/STAT3 signaling pathway

    Journal: Cancer Cell International

    doi: 10.1186/s12935-024-03270-z

    SLC25A17 knockdown inhibited TNBC cell proliferation. A SLC25A17 mRNA and protein expression were higher in TNBC cell lines than in breast epithelial cell. B, C CCK8 and EdU assays were performed to evaluate the effect of SLC25A17 knockdown on proliferative capacity in MDA-MB-231 and MDA-MB-468 cells. D The apoptosis level was assessed by flow cytometric analysis in MDA-MB-231 and MDA-MB-468 cells. E Colony formation assays were performed to evaluate the proliferative capacity in MDA-MB-231 and MDA-MB-468 cells. F The expression levels of apoptosis-related and cell cycle-related proteins were detected by western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: SLC25A17 knockdown inhibited TNBC cell proliferation. A SLC25A17 mRNA and protein expression were higher in TNBC cell lines than in breast epithelial cell. B, C CCK8 and EdU assays were performed to evaluate the effect of SLC25A17 knockdown on proliferative capacity in MDA-MB-231 and MDA-MB-468 cells. D The apoptosis level was assessed by flow cytometric analysis in MDA-MB-231 and MDA-MB-468 cells. E Colony formation assays were performed to evaluate the proliferative capacity in MDA-MB-231 and MDA-MB-468 cells. F The expression levels of apoptosis-related and cell cycle-related proteins were detected by western blot. * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Expressing, Western Blot

    SLC25A17 knockdown inhibited migration, invasion, and EMT of TNBC cells. A Representative transwell migration and invasion images and statistics for the number of migrated and invaded TNBC cells with SLC25A17 knockdown. (scale bar: 100 μm). B Representative pictures and quantitative data of wound healing assay in MDA-MB-231 and MDA-MB-468 with SLC25A17 knockdown. C Immunofluorescence staining analysis of EMT markers of E-Cadherin and Vimentin (red), and the nuclei were stained with DAPI (blue) (scale bar: 100 μm). D Invasion-related proteins, E-Cadherin, Vimentin, MMP9 and MMP2 were detected by western blot analysis. * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: SLC25A17 knockdown inhibited migration, invasion, and EMT of TNBC cells. A Representative transwell migration and invasion images and statistics for the number of migrated and invaded TNBC cells with SLC25A17 knockdown. (scale bar: 100 μm). B Representative pictures and quantitative data of wound healing assay in MDA-MB-231 and MDA-MB-468 with SLC25A17 knockdown. C Immunofluorescence staining analysis of EMT markers of E-Cadherin and Vimentin (red), and the nuclei were stained with DAPI (blue) (scale bar: 100 μm). D Invasion-related proteins, E-Cadherin, Vimentin, MMP9 and MMP2 were detected by western blot analysis. * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Migration, Wound Healing Assay, Immunofluorescence, Staining, Western Blot

    ROS induced by SLC25A17 knockdown promoted apoptosis and autophagy in TNBC cells. A Flow cytometric analysis and quantification of ROS production in MDA-MB-231 and MDA-MB-468 cells. B The level of ROS was observed by fluorescence microscopy in SLC25A17 knockdown or overexpression MDA-MB-231 and MDA-MB-468 cells. (scale bar: 100 μm). C The protein levels of autophagy and apoptosis-related proteins was detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without NAC (5 mM). * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: ROS induced by SLC25A17 knockdown promoted apoptosis and autophagy in TNBC cells. A Flow cytometric analysis and quantification of ROS production in MDA-MB-231 and MDA-MB-468 cells. B The level of ROS was observed by fluorescence microscopy in SLC25A17 knockdown or overexpression MDA-MB-231 and MDA-MB-468 cells. (scale bar: 100 μm). C The protein levels of autophagy and apoptosis-related proteins was detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without NAC (5 mM). * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Fluorescence, Microscopy, Over Expression, Western Blot

    SLC25A17 knockdown inhibited JAK2/STAT3 pathway in TNBC cells. A The protein levels of phosphorylated STAT3 (p-STAT3) and phosphorylated JAK2 (p-JAK2) was detected by western blot in MDA-MB-231 and MDA-MB-468 cells. B The protein levels of p-STAT3 and its downstream protein Cyclin D and Bcl-2 were detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without NAC (5 mM). C The protein levels of autophagy and apoptosis-related proteins was detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without IL-6 (50 ng/mL). D Colony formation in SLC25A17 knockdown MDA-MB-231 cells with or without IL-6 (50 ng/mL). E Representative transwell invasion images and statistics for the number of invaded cells in SLC25A17 knockdown MDA-MB-231 cells with or without IL-6 (50 ng/mL). * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: SLC25A17 knockdown inhibited JAK2/STAT3 pathway in TNBC cells. A The protein levels of phosphorylated STAT3 (p-STAT3) and phosphorylated JAK2 (p-JAK2) was detected by western blot in MDA-MB-231 and MDA-MB-468 cells. B The protein levels of p-STAT3 and its downstream protein Cyclin D and Bcl-2 were detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without NAC (5 mM). C The protein levels of autophagy and apoptosis-related proteins was detected by western blot in MDA-MB-231 and MDA-MB-468 cells treated with or without IL-6 (50 ng/mL). D Colony formation in SLC25A17 knockdown MDA-MB-231 cells with or without IL-6 (50 ng/mL). E Representative transwell invasion images and statistics for the number of invaded cells in SLC25A17 knockdown MDA-MB-231 cells with or without IL-6 (50 ng/mL). * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Western Blot

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    ATCC mda mb 468
    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , <t>MM468</t> wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Mda Mb 468, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda mb 468/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mda mb 468 - by Bioz Stars, 2024-03
    86/100 stars
      Buy from Supplier

    86
    ATCC human bc cell lines mda mb 231
    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in <t>MM231</t> wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Human Bc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human bc cell lines mda mb 231/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human bc cell lines mda mb 231 - by Bioz Stars, 2024-03
    86/100 stars
      Buy from Supplier

    86
    ATCC crl 1472 fbs 2 mda mb breast atcc adherent leibovitz s l 468 htb
    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in <t>MM231</t> wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Crl 1472 Fbs 2 Mda Mb Breast Atcc Adherent Leibovitz S L 468 Htb, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/crl 1472 fbs 2 mda mb breast atcc adherent leibovitz s l 468 htb/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    crl 1472 fbs 2 mda mb breast atcc adherent leibovitz s l 468 htb - by Bioz Stars, 2024-03
    86/100 stars
      Buy from Supplier

    86
    ATCC mda mb 231
    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in <t>MM231</t> wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mda mb 231/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mda mb 231 - by Bioz Stars, 2024-03
    86/100 stars
      Buy from Supplier

    86
    ATCC fbs 2 mda mb breast atcc adherent leibovitz s l
    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in <t>MM231</t> wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Fbs 2 Mda Mb Breast Atcc Adherent Leibovitz S L, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fbs 2 mda mb breast atcc adherent leibovitz s l/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fbs 2 mda mb breast atcc adherent leibovitz s l - by Bioz Stars, 2024-03
    86/100 stars
      Buy from Supplier

    86
    ATCC bc cell line mda mb 231
    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in <t>MM231</t> wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Bc Cell Line Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bc cell line mda mb 231/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bc cell line mda mb 231 - by Bioz Stars, 2024-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.

    Journal: Oncology Letters

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    doi: 10.3892/ol.2024.14291

    Figure Lengend Snippet: (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.

    Article Snippet: Human BC cell lines MDA-MB-231 (MM231; HTB-26), MDA-MB-468 (MM468; HTB-132), and HCC1395 (CRL-2324) were purchased from the American Type Culture Collection.

    Techniques: Expressing, Microarray

    (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.

    Journal: Oncology Letters

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    doi: 10.3892/ol.2024.14291

    Figure Lengend Snippet: (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.

    Article Snippet: Human BC cell lines MDA-MB-231 (MM231; HTB-26), MDA-MB-468 (MM468; HTB-132), and HCC1395 (CRL-2324) were purchased from the American Type Culture Collection.

    Techniques: Real-time Polymerase Chain Reaction

    (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.

    Journal: Oncology Letters

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    doi: 10.3892/ol.2024.14291

    Figure Lengend Snippet: (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.

    Article Snippet: Human BC cell lines MDA-MB-231 (MM231; HTB-26), MDA-MB-468 (MM468; HTB-132), and HCC1395 (CRL-2324) were purchased from the American Type Culture Collection.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Incubation

    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.

    Journal: Oncology Letters

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    doi: 10.3892/ol.2024.14291

    Figure Lengend Snippet: (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.

    Article Snippet: Human BC cell lines MDA-MB-231 (MM231; HTB-26), MDA-MB-468 (MM468; HTB-132), and HCC1395 (CRL-2324) were purchased from the American Type Culture Collection.

    Techniques: Expressing, Microarray

    (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.

    Journal: Oncology Letters

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    doi: 10.3892/ol.2024.14291

    Figure Lengend Snippet: (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.

    Article Snippet: Human BC cell lines MDA-MB-231 (MM231; HTB-26), MDA-MB-468 (MM468; HTB-132), and HCC1395 (CRL-2324) were purchased from the American Type Culture Collection.

    Techniques: Real-time Polymerase Chain Reaction

    (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.

    Journal: Oncology Letters

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    doi: 10.3892/ol.2024.14291

    Figure Lengend Snippet: (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.

    Article Snippet: Human BC cell lines MDA-MB-231 (MM231; HTB-26), MDA-MB-468 (MM468; HTB-132), and HCC1395 (CRL-2324) were purchased from the American Type Culture Collection.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Incubation