breast cancer cell line mda mb  (ATCC)

Journal: PLoS ONE

Article Title: A Novel Network Integrating a miRNA-203/SNAI1 Feedback Loop which Regulates Epithelial to Mesenchymal Transition

doi: 10.1371/journal.pone.0035440

Figure Lengend Snippet: HTB129-ctrl and HTB129-miR203 cells were subjected to A) qRT-PCR analyses of SNAI1 mRNA expression in HTB129-ctrl and HTB129-miR203 cells, B) fluorescent staining with DAPI (blue) and phalloidin (red) (scale bar, 20 µm), C) migration and D) invasion assays. E) Predicted miR-203 target sites within SNAI1 3′UTR. F, G) Relative luciferase activity of SNAI1-3′UTR wild type (wt) or mutant (mut) in MDA231 cells transfected with control (F, G), miR-203 (F) or miR-200a/c (G) precursors. Co-transfection with GAPDH 3′UTR vector served as additional negative control (*, p<0.05; **, p<0.01).

Article Snippet: The human breast cancer cell lines HTB129 and MDA231 (also named MDA-MB-231 or HTB-26), purchased from the ATCC, were maintained in RPMI1640 and Leibovitz culture media (Lonza), respectively, supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/ml penicillin and 100 µg/ml streptomycin.

Techniques: Quantitative RT-PCR, Expressing, Staining, Migration, Luciferase, Activity Assay, Mutagenesis, Transfection, Cotransfection, Plasmid Preparation, Negative Control

Journal: PLoS ONE

Article Title: Links between the Oncoprotein YB-1 and Small Non-Coding RNAs in Breast Cancer

doi: 10.1371/journal.pone.0080171

Figure Lengend Snippet: A . Experion Bioanalyser gel image for RNA isolated following immunoprecipitation with YB-1 antibody or IgG isotype control antibody (labelled YB-1 IP and IgG IP respectively) from MDA-MB-435S cells. Ribosomal RNAs (18S and 28S) are shown by arrows. The ladder shows the size of the RNAs in nucleotides. Small RNAs are highlighted by the labelled bracket. B . RT-qPCR detection of mRNAs bound following immunoprecipitation with the YB-1 antibody.

Article Snippet: MCF7 and MDA-MB-435S breast cancer cell lines were purchased from the American Type Culture Collection (Manassas, VA).

Techniques: Isolation, Immunoprecipitation, Quantitative RT-PCR

Journal: PLoS ONE

Article Title: Links between the Oncoprotein YB-1 and Small Non-Coding RNAs in Breast Cancer

doi: 10.1371/journal.pone.0080171

Figure Lengend Snippet: sncRNAs that are bound by YB-1 protein by immunoprecipitation in MCF7 and MDA-MB-435S cells based on enrichment in abundance in IP over input.

Article Snippet: MCF7 and MDA-MB-435S breast cancer cell lines were purchased from the American Type Culture Collection (Manassas, VA).

Techniques: Immunoprecipitation

Journal: PLoS ONE

Article Title: Links between the Oncoprotein YB-1 and Small Non-Coding RNAs in Breast Cancer

doi: 10.1371/journal.pone.0080171

Figure Lengend Snippet: A. MCF7 cells B. MDA-MB-435S cells Note: miR-886 abundance in MCF7 cells was undetectable.

Article Snippet: MCF7 and MDA-MB-435S breast cancer cell lines were purchased from the American Type Culture Collection (Manassas, VA).

Techniques:

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: MiR-27a Modulates Radiosensitivity of Triple-Negative Breast Cancer (TNBC) Cells by Targeting CDC27

doi: 10.12659/MSM.893974

Figure Lengend Snippet: MiR-27a is significantly increased and CDC27 expression is significantly decreased in TNBC cells. ( A ) qRT-PCR analysis of miR-27a expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. ( B ) qRT-PCR analysis of CDC27 mRNA expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. ( C ) Western blot analysis of CDC27 expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. Data are shown as mean±S.D by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.

Article Snippet: Triple negative breast cancer cell line MDA-MB-435 and MDA-MB-231, normal human breast epithelial cell line MCF10A and HEK293T cells were obtained from ATCC.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: MiR-27a Modulates Radiosensitivity of Triple-Negative Breast Cancer (TNBC) Cells by Targeting CDC27

doi: 10.12659/MSM.893974

Figure Lengend Snippet: MiR-27a is involved in radiosensitivity of TNBC cells. ( A–D ) qRT-PCR analysis of miR-27a expression in MDA-MB-435 ( A, C ) MDA-MB-231 ( B, D ) cells after transfection of 200nM antagomiR-27a ( A, B ) 75nM miR-27a mimics (C, D). ( D–G ) CCK-8 assay of cell proliferation of MDA-MB-435 ( D, F ) MDA-MB-231 ( E, G ) cells transfected with 200 nM antagomiR-27a with/without treatment of IR (8 Gy) (E, F) or transfected with 75 nM miR-27a mimics with/without treatment of IR (8 Gy) ( G, H ). ( I, J ) MDA-MB-435 cells with miR-27a knockdown or overexpression were stained with caspase-3 staining kit 24 h after IR (8 Gy) treatment. Then, flow cytometry analysis was performed to measure the proportion of cells with active caspase-3 staining. Representative flow cytometry images of the cells with active caspase-3 ( I ). Quantification of apoptotic cells with active caspase-3 signal in ( J ). Data are shown as mean ±S.D. by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.

Article Snippet: Triple negative breast cancer cell line MDA-MB-435 and MDA-MB-231, normal human breast epithelial cell line MCF10A and HEK293T cells were obtained from ATCC.

Techniques: Quantitative RT-PCR, Expressing, Transfection, CCK-8 Assay, Over Expression, Staining, Flow Cytometry

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: MiR-27a Modulates Radiosensitivity of Triple-Negative Breast Cancer (TNBC) Cells by Targeting CDC27

doi: 10.12659/MSM.893974

Figure Lengend Snippet: MiR-27a directly targets CDC27 and regulates its expression in TNBC cells. ( A ) The putative binding site between miR-27a and CDC27 and the designed mutant sequence without the pairing. ( B, C ) The relative firefly luciferase activity in HEK-293T ( B ) and MDA-MB-435 ( C ) cells co-transfected with 150 ng reporter plasmids and 50 nM miR-27a mimics. Both firefly and Renilla luciferase activities were measured 24 h after transfection and the firefly luciferase activity was normalized to the Renilla luciferase activity. ( D, E ) Western blot analysis of CDC27 protein expression in MDA-MB-435 transfected with miR-27a mimics or siCDC-27 ( D ) or transfected with antagomiR-27a or infected with CDC-27 lentiviral particles ( E ). Data are shown as mean ±S.D. by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.

Article Snippet: Triple negative breast cancer cell line MDA-MB-435 and MDA-MB-231, normal human breast epithelial cell line MCF10A and HEK293T cells were obtained from ATCC.

Techniques: Expressing, Binding Assay, Mutagenesis, Sequencing, Luciferase, Activity Assay, Transfection, Western Blot, Infection

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: MiR-27a Modulates Radiosensitivity of Triple-Negative Breast Cancer (TNBC) Cells by Targeting CDC27

doi: 10.12659/MSM.893974

Figure Lengend Snippet: MiR-27a modulates radiosensitivity of TNBC cells through targeting CDC27. ( A–D ) CCK-8 assay of cell proliferation in MDA-MB-435 ( A, C ) MDA-MB-231 ( B, D ) cells after infection with CDC27 lentiviral particle with/without treatment of IR (8 Gy) ( A, B ) or transfected with 50 nM siCDC27 with/without treatment of IR (8 Gy) ( C, D ). ( E, F ) The effect of co-transfection of miR-27a and CDC27 on cell proliferation in MDA-MB-435 ( E ) MDA-MB-231 ( F ) cells with/without treatment of IR (8 Gy). ( G, H ) The effect of co-transfection of antagomiR-27a and siCDC27 on cell proliferation in MDA-MB-435 ( G ) MDA-MB-231 ( H ) cells with/without treatment of IR (8 Gy). Data are shown as mean ±S.D. by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.

Article Snippet: Triple negative breast cancer cell line MDA-MB-435 and MDA-MB-231, normal human breast epithelial cell line MCF10A and HEK293T cells were obtained from ATCC.

Techniques: CCK-8 Assay, Infection, Transfection, Cotransfection