mda mb 435 cell lines  (ATCC)


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    ATCC mda mb 435 cell lines
    ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an <t>MDA-MB-435</t> orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).
    Mda Mb 435 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Non-Invasive Imaging of Tumors by Monitoring Autotaxin Activity Using an Enzyme-Activated Near-Infrared Fluorogenic Substrate"

    Article Title: Non-Invasive Imaging of Tumors by Monitoring Autotaxin Activity Using an Enzyme-Activated Near-Infrared Fluorogenic Substrate

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0079065

    ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an MDA-MB-435 orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).
    Figure Legend Snippet: ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an MDA-MB-435 orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).

    Techniques Used: Injection, In Vivo, Fluorescence, Enzyme-linked Immunosorbent Assay

    cell line mda mb 435s  (ATCC)


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    ATCC cell line mda mb 435s
    Cell Line Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human melanoma cell line mda mb 435s  (ATCC)


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    ATCC human melanoma cell line mda mb 435s
    Human Melanoma Cell Line Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human melanoma cell line mda mb 435s  (ATCC)


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    ATCC human melanoma cell line mda mb 435s
    KANK2, unlike KANK1, is localized within αVβ5 FAs. A Talin1 and talin2 are localized within integrin αVβ5 FAs. Forty-eight hours after seeding <t>MDA-MB-435S</t> cells were methanol fixed, and stained with anti-talin1 antibody followed by Alexa-Fluor IgG1 555-conjugated antibody (red), anti-talin2 antibody followed by Alexa-Fluor IgG2b 488-conjugated antibody (green) and anti-integrin β5 antibody followed by Alexa-Fluor 647-conjugated antibody (magenta) and interference reflection microscopy (IRM) images were taken. B , C KANK2, unlike KANK1, localizes in talin1 and talin2-positive FAs. Forty-eight hours after seeding MDA-MB-435S cells were methanol fixed and stained with anti-talin1 or anti-talin2 antibody followed by Alexa-Fluor 546-conjugated antibody (red) or Alexa-Fluor 488-conjugated antibody (green) and anti-KANK1 or anti-KANK2 antibody followed by Alexa-Fluor 555-conjugated antibody or Alexa-Fluor 488-conjugated antibody (shown in magenta). Vinculin was visualized using conjugated anti-vinculin Alexa Fluor 647 antibody (shown in grey) and IRM images were taken. Analysis was performed using TCS SP8 Leica. Scale bar = 10 µm
    Human Melanoma Cell Line Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Talin2 and KANK2 functionally interact to regulate microtubule dynamics, paclitaxel sensitivity and cell migration in the MDA-MB-435S melanoma cell line"

    Article Title: Talin2 and KANK2 functionally interact to regulate microtubule dynamics, paclitaxel sensitivity and cell migration in the MDA-MB-435S melanoma cell line

    Journal: Cellular & Molecular Biology Letters

    doi: 10.1186/s11658-023-00473-6

    KANK2, unlike KANK1, is localized within αVβ5 FAs. A Talin1 and talin2 are localized within integrin αVβ5 FAs. Forty-eight hours after seeding MDA-MB-435S cells were methanol fixed, and stained with anti-talin1 antibody followed by Alexa-Fluor IgG1 555-conjugated antibody (red), anti-talin2 antibody followed by Alexa-Fluor IgG2b 488-conjugated antibody (green) and anti-integrin β5 antibody followed by Alexa-Fluor 647-conjugated antibody (magenta) and interference reflection microscopy (IRM) images were taken. B , C KANK2, unlike KANK1, localizes in talin1 and talin2-positive FAs. Forty-eight hours after seeding MDA-MB-435S cells were methanol fixed and stained with anti-talin1 or anti-talin2 antibody followed by Alexa-Fluor 546-conjugated antibody (red) or Alexa-Fluor 488-conjugated antibody (green) and anti-KANK1 or anti-KANK2 antibody followed by Alexa-Fluor 555-conjugated antibody or Alexa-Fluor 488-conjugated antibody (shown in magenta). Vinculin was visualized using conjugated anti-vinculin Alexa Fluor 647 antibody (shown in grey) and IRM images were taken. Analysis was performed using TCS SP8 Leica. Scale bar = 10 µm
    Figure Legend Snippet: KANK2, unlike KANK1, is localized within αVβ5 FAs. A Talin1 and talin2 are localized within integrin αVβ5 FAs. Forty-eight hours after seeding MDA-MB-435S cells were methanol fixed, and stained with anti-talin1 antibody followed by Alexa-Fluor IgG1 555-conjugated antibody (red), anti-talin2 antibody followed by Alexa-Fluor IgG2b 488-conjugated antibody (green) and anti-integrin β5 antibody followed by Alexa-Fluor 647-conjugated antibody (magenta) and interference reflection microscopy (IRM) images were taken. B , C KANK2, unlike KANK1, localizes in talin1 and talin2-positive FAs. Forty-eight hours after seeding MDA-MB-435S cells were methanol fixed and stained with anti-talin1 or anti-talin2 antibody followed by Alexa-Fluor 546-conjugated antibody (red) or Alexa-Fluor 488-conjugated antibody (green) and anti-KANK1 or anti-KANK2 antibody followed by Alexa-Fluor 555-conjugated antibody or Alexa-Fluor 488-conjugated antibody (shown in magenta). Vinculin was visualized using conjugated anti-vinculin Alexa Fluor 647 antibody (shown in grey) and IRM images were taken. Analysis was performed using TCS SP8 Leica. Scale bar = 10 µm

    Techniques Used: Staining, Microscopy

    Talin1, unlike talin2, is necessary for formation of integrin αVβ5 FAs. A Talin1 knockdown, unlike talin2, reduces the level of KANK2 in whole cell lysates, while KANK1 level does not change upon either talin1 or talin2 knockdown. WB analysis of talin1, talin2, KANK1 or KANK2 in MDA-MB-435S cells transfected with either control, talin1, talin2 or a combination of talin1 and talin2-specific siRNAs and in the 3αV clone with decreased expression of integrin αV. Forty-eight hours after transfection total cell lysates were collected and WB analysis was performed. The results presented are representative of three independent experiments with similar results. B Quantification of data presented in ( A ). Histogram data are plotted as mean ± SD ( n ≥ 3) relative to expression in MDA-MB-435S cells transfected with control siRNA that was set as 1 (indicated by a dotted line). Data were analyzed by one-way ANOVA with Dunnett’s multiple comparison. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. C Knockdown of talin1 leads to disruption of αVβ5 FAs. Forty-eight hours after transfection with either control siRNA, talin1 or talin2-specific siRNA, MDA-MB-435S cells were methanol fixed, and stained with anti-talin1 antibody followed by Alexa-Fluor IgG1 555-conjugated antibody (red), anti-talin2 antibody followed by Alexa-Fluor IgG2b 488-conjugated antibody (green) and anti-β5 antibody followed by Alexa-Fluor 647-conjugated antibody (magenta) and IRM images were taken. Analysis was performed using TCS SP8 Leica. Scale bar = 10 µm. D Quantification of data presented in ( C ). Violin plots (number of structures/cell) and scatter plots with median marked in size (size of structures/cell) represents measurements of > 45 cells. Data were analyzed by one-way ANOVA with Dunnett’s multiple comparison. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001
    Figure Legend Snippet: Talin1, unlike talin2, is necessary for formation of integrin αVβ5 FAs. A Talin1 knockdown, unlike talin2, reduces the level of KANK2 in whole cell lysates, while KANK1 level does not change upon either talin1 or talin2 knockdown. WB analysis of talin1, talin2, KANK1 or KANK2 in MDA-MB-435S cells transfected with either control, talin1, talin2 or a combination of talin1 and talin2-specific siRNAs and in the 3αV clone with decreased expression of integrin αV. Forty-eight hours after transfection total cell lysates were collected and WB analysis was performed. The results presented are representative of three independent experiments with similar results. B Quantification of data presented in ( A ). Histogram data are plotted as mean ± SD ( n ≥ 3) relative to expression in MDA-MB-435S cells transfected with control siRNA that was set as 1 (indicated by a dotted line). Data were analyzed by one-way ANOVA with Dunnett’s multiple comparison. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. C Knockdown of talin1 leads to disruption of αVβ5 FAs. Forty-eight hours after transfection with either control siRNA, talin1 or talin2-specific siRNA, MDA-MB-435S cells were methanol fixed, and stained with anti-talin1 antibody followed by Alexa-Fluor IgG1 555-conjugated antibody (red), anti-talin2 antibody followed by Alexa-Fluor IgG2b 488-conjugated antibody (green) and anti-β5 antibody followed by Alexa-Fluor 647-conjugated antibody (magenta) and IRM images were taken. Analysis was performed using TCS SP8 Leica. Scale bar = 10 µm. D Quantification of data presented in ( C ). Violin plots (number of structures/cell) and scatter plots with median marked in size (size of structures/cell) represents measurements of > 45 cells. Data were analyzed by one-way ANOVA with Dunnett’s multiple comparison. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

    Techniques Used: Transfection, Expressing, Staining

    Talin2 functionally interacts with KANK2. A Knockdown of talin1, unlike talin2, leads to changes in KANK2 localization. Forty-eight hours after transfection with either control siRNA, talin1 or talin2-specific siRNA, MDA-MB-435S cells were methanol fixed and stained with anti-talin1 antibody followed by Alexa-Fluor IgG1 555-conjugated antibody (red), anti-talin2 antibody followed by Alexa-Fluor IgG2b 488-conjugated antibody (green), anti-KANK2 antibody followed by Alexa-Fluor 647-conjugated antibody (magenta) and IRM images were taken. Analysis was performed using TCS SP8 Leica. Scale bar = 10 µm. B Quantification of data presented in ( A ). Violin plot represents measurements of > 30 cells. Data were analyzed by one-way ANOVA with Dunnett’s multiple comparison. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. C WB analysis of talin1, talin2, liprin-β1, KANK2 or integrin β5 in IACs isolated from MDA-MB-435S cells transfected with either control siRNA, talin1 or talin2-specific siRNA. Forty-eight hours after transfection, IACs were isolated and WB analysis was performed. The results presented are representative of two independent experiments yielding similar results. Numbers below WB scan represent expression of target proteins after talin1 or talin2 knockdown as relative to expression of target proteins in cell transfected with control siRNA set as 1
    Figure Legend Snippet: Talin2 functionally interacts with KANK2. A Knockdown of talin1, unlike talin2, leads to changes in KANK2 localization. Forty-eight hours after transfection with either control siRNA, talin1 or talin2-specific siRNA, MDA-MB-435S cells were methanol fixed and stained with anti-talin1 antibody followed by Alexa-Fluor IgG1 555-conjugated antibody (red), anti-talin2 antibody followed by Alexa-Fluor IgG2b 488-conjugated antibody (green), anti-KANK2 antibody followed by Alexa-Fluor 647-conjugated antibody (magenta) and IRM images were taken. Analysis was performed using TCS SP8 Leica. Scale bar = 10 µm. B Quantification of data presented in ( A ). Violin plot represents measurements of > 30 cells. Data were analyzed by one-way ANOVA with Dunnett’s multiple comparison. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. C WB analysis of talin1, talin2, liprin-β1, KANK2 or integrin β5 in IACs isolated from MDA-MB-435S cells transfected with either control siRNA, talin1 or talin2-specific siRNA. Forty-eight hours after transfection, IACs were isolated and WB analysis was performed. The results presented are representative of two independent experiments yielding similar results. Numbers below WB scan represent expression of target proteins after talin1 or talin2 knockdown as relative to expression of target proteins in cell transfected with control siRNA set as 1

    Techniques Used: Transfection, Staining, Isolation, Expressing

    Talin1 or talin2 knockdown affects actin and MT cytoskeleton . A Visualization of α-tubulin and F-actin upon knockdown of talin1 or talin2. Forty-eight hours after transfection with either control, talin1 or talin2-specific siRNA, MDA-MB-435S cells were fixed with methanol (for α-tubulin visualization) or PFA followed by permeabilization with Triton X-100 (for F-actin visualization), and stained with anti-talin1 antibody followed by Alexa-Fluor IgG1 555-conjugated antibody (red), anti-talin2 antibody followed by Alexa-Fluor IgG2b 488-conjugated antibody (green), anti-α-tubulin antibody followed by Alexa-Flour 647-conjugated antibody (magenta) and IRM images were taken. For F-actin visualization cells were incubated with Alexa-Flour 488 conjugated phalloidin (shown in gold). Analysis was performed using TCS SP8 Leica. Scale bar = 10 µm. B , C Quantification of data presented in ( A ). Violin plots represents measurements of > 30 cells. Data were analyzed by unpaired Student’s t -test. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001
    Figure Legend Snippet: Talin1 or talin2 knockdown affects actin and MT cytoskeleton . A Visualization of α-tubulin and F-actin upon knockdown of talin1 or talin2. Forty-eight hours after transfection with either control, talin1 or talin2-specific siRNA, MDA-MB-435S cells were fixed with methanol (for α-tubulin visualization) or PFA followed by permeabilization with Triton X-100 (for F-actin visualization), and stained with anti-talin1 antibody followed by Alexa-Fluor IgG1 555-conjugated antibody (red), anti-talin2 antibody followed by Alexa-Fluor IgG2b 488-conjugated antibody (green), anti-α-tubulin antibody followed by Alexa-Flour 647-conjugated antibody (magenta) and IRM images were taken. For F-actin visualization cells were incubated with Alexa-Flour 488 conjugated phalloidin (shown in gold). Analysis was performed using TCS SP8 Leica. Scale bar = 10 µm. B , C Quantification of data presented in ( A ). Violin plots represents measurements of > 30 cells. Data were analyzed by unpaired Student’s t -test. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

    Techniques Used: Transfection, Staining, Incubation

    Disruption of crosstalk between CMSCs and FAs leads to increased velocity of microtubule growth and increased sensitivity to PTX. A Knockdown of αV integrin subunit increases velocity of microtubule growth. Quantification of time-lapse live cell microscopy data of 435S-EB3 cells and 3αV-EB3 cell clone with decreased expression of integrin αV. Violin plot represents measurements of > 450 analyzed microtubules, ( n = 3) relative to velocity of MT growth in MDA-MB-435S cells that was set as 1. Data were analyzed by unpaired Student’s t -test. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Still images of Additional file : Movie S1 and Additional file : S2 represent tracking of one microtubule tip in 104 s through five frames. (B) 435S-EB3 cells transfected with either talin1, talin2, KANK2 or β5-specific siRNA, but not KANK1 siRNA, showed a significant increase in velocity of MT growth compared to cells transfected with control siRNA. Quantification of time-lapse live cell microscopy data. Violin plot represents measurement of > 250 analyzed microtubules, ( n ≥ 2) relative to velocity of MT growth in MDA-MB-435S cells transfected with control siRNA that was set as 1. Data were analyzed by one-way ANOVA with Šídák’s multiple comparisons test. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. C PTX treatment decreases MT growth velocity much stronger in 3αV-EB3 cells compared to 435S-EB3 cells. Quantification of time-lapse live cell microscopy data of 435S-EB3 cells and 3αV-EB3 clone upon treatment with equitoxic and equimolar concentrations of PTX. Scatter plot with median marked in red represents measurements of > 200 analyzed microtubules, ( n = 3) relative to velocity of MT growth in MDA-MB-435S cells that was set as 1. Data were analyzed by one-way ANOVA with Šídák’s multiple comparisons test. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. D Cell proliferation decreases upon knockdown of talin1, but not talin2, KANK1 or KANK2. Cell proliferation was measured using ClickIT EdU assay upon transfection with either control, talin1, talin2, combination of talin1 and talin2, KANK1 or KANK2-specific siRNA. Histogram represents measurements of > 30 cells, plotted as mean ± SD ( n = 2). Data were analyzed by one-way ANOVA with Dunnett’s multiple comparison. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. E Talin2 knockdown increases sensitivity to PTX in MDA-MB-435S cells. Twenty-four hours upon transfection, cells were seeded in 96-well plates and 24 h later treated with different concentrations of PTX. Cytotoxicity was measured by MTT assay. Data were analyzed by two-way analysis of variance (ANOVA) with Šídák’s multiple comparisons test, with a single pooled variance. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ( n = 3). F Talin2 knockdown decreases migration in MDA-MB-435S cells. Serum starved (24 h) cells, transfected previously with either control or talin2-specific siRNA were seeded in Transwell cell culture inserts and left to migrate for 22 h toward serum. Cells on the underside of the inserts were stained with crystal violet, photographed, and counted. Scale bar = 100 µm. F Histogram data represents averages of five microscope fields of three independently performed experiments, plotted as mean ± SD. Data were analyzed by unpaired Student’s t -test. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001
    Figure Legend Snippet: Disruption of crosstalk between CMSCs and FAs leads to increased velocity of microtubule growth and increased sensitivity to PTX. A Knockdown of αV integrin subunit increases velocity of microtubule growth. Quantification of time-lapse live cell microscopy data of 435S-EB3 cells and 3αV-EB3 cell clone with decreased expression of integrin αV. Violin plot represents measurements of > 450 analyzed microtubules, ( n = 3) relative to velocity of MT growth in MDA-MB-435S cells that was set as 1. Data were analyzed by unpaired Student’s t -test. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Still images of Additional file : Movie S1 and Additional file : S2 represent tracking of one microtubule tip in 104 s through five frames. (B) 435S-EB3 cells transfected with either talin1, talin2, KANK2 or β5-specific siRNA, but not KANK1 siRNA, showed a significant increase in velocity of MT growth compared to cells transfected with control siRNA. Quantification of time-lapse live cell microscopy data. Violin plot represents measurement of > 250 analyzed microtubules, ( n ≥ 2) relative to velocity of MT growth in MDA-MB-435S cells transfected with control siRNA that was set as 1. Data were analyzed by one-way ANOVA with Šídák’s multiple comparisons test. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. C PTX treatment decreases MT growth velocity much stronger in 3αV-EB3 cells compared to 435S-EB3 cells. Quantification of time-lapse live cell microscopy data of 435S-EB3 cells and 3αV-EB3 clone upon treatment with equitoxic and equimolar concentrations of PTX. Scatter plot with median marked in red represents measurements of > 200 analyzed microtubules, ( n = 3) relative to velocity of MT growth in MDA-MB-435S cells that was set as 1. Data were analyzed by one-way ANOVA with Šídák’s multiple comparisons test. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. D Cell proliferation decreases upon knockdown of talin1, but not talin2, KANK1 or KANK2. Cell proliferation was measured using ClickIT EdU assay upon transfection with either control, talin1, talin2, combination of talin1 and talin2, KANK1 or KANK2-specific siRNA. Histogram represents measurements of > 30 cells, plotted as mean ± SD ( n = 2). Data were analyzed by one-way ANOVA with Dunnett’s multiple comparison. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. E Talin2 knockdown increases sensitivity to PTX in MDA-MB-435S cells. Twenty-four hours upon transfection, cells were seeded in 96-well plates and 24 h later treated with different concentrations of PTX. Cytotoxicity was measured by MTT assay. Data were analyzed by two-way analysis of variance (ANOVA) with Šídák’s multiple comparisons test, with a single pooled variance. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ( n = 3). F Talin2 knockdown decreases migration in MDA-MB-435S cells. Serum starved (24 h) cells, transfected previously with either control or talin2-specific siRNA were seeded in Transwell cell culture inserts and left to migrate for 22 h toward serum. Cells on the underside of the inserts were stained with crystal violet, photographed, and counted. Scale bar = 100 µm. F Histogram data represents averages of five microscope fields of three independently performed experiments, plotted as mean ± SD. Data were analyzed by unpaired Student’s t -test. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

    Techniques Used: Microscopy, Expressing, Transfection, EdU Assay, MTT Assay, Migration, Cell Culture, Staining

    Schematic representation of the functional link between talin2 from αVβ5 FAs and KANK2 from CMSC. Disruption of the link, either by KANK2 or talin2 knockdown, results in increased velocity of MT growth, increased sensitivity to PTX and decreased migration of MDA-MB-435S cells
    Figure Legend Snippet: Schematic representation of the functional link between talin2 from αVβ5 FAs and KANK2 from CMSC. Disruption of the link, either by KANK2 or talin2 knockdown, results in increased velocity of MT growth, increased sensitivity to PTX and decreased migration of MDA-MB-435S cells

    Techniques Used: Functional Assay, Migration

    breast cancer cell line mda mb 435  (ATCC)


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    ATCC breast cancer cell line mda mb 435
    Breast Cancer Cell Line Mda Mb 435, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    breast cancer cell line mda mb 435  (ATCC)


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    ATCC breast cancer cell line mda mb 435
    Breast Cancer Cell Line Mda Mb 435, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human melanoma cell line mda mb 435s  (ATCC)


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    ATCC human melanoma cell line mda mb 435s
    Human Melanoma Cell Line Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human breast cancer cells mda mb 435 melanoma cell line meoh methanol mgc  (ATCC)


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    ATCC human breast cancer cells mda mb 435 melanoma cell line meoh methanol mgc
    Human Breast Cancer Cells Mda Mb 435 Melanoma Cell Line Meoh Methanol Mgc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mda mb 435 cell lines  (ATCC)


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    ATCC mda mb 435 cell lines
    ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an <t>MDA-MB-435</t> orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).
    Mda Mb 435 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Non-Invasive Imaging of Tumors by Monitoring Autotaxin Activity Using an Enzyme-Activated Near-Infrared Fluorogenic Substrate"

    Article Title: Non-Invasive Imaging of Tumors by Monitoring Autotaxin Activity Using an Enzyme-Activated Near-Infrared Fluorogenic Substrate

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0079065

    ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an MDA-MB-435 orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).
    Figure Legend Snippet: ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an MDA-MB-435 orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).

    Techniques Used: Injection, In Vivo, Fluorescence, Enzyme-linked Immunosorbent Assay

    ductal carcinoma cell line mda mb 435s  (ATCC)


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    ATCC ductal carcinoma cell line mda mb 435s
    Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, <t>MDA-MB</t> <t>435S)</t> were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.
    Ductal Carcinoma Cell Line Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli"

    Article Title: High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

    Journal: Microbial Cell Factories

    doi: 10.1186/1475-2859-12-97

    Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, MDA-MB 435S) were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.
    Figure Legend Snippet: Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, MDA-MB 435S) were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.

    Techniques Used: Expressing, Binding Assay, SDS Page, Western Blot, Incubation, In Vitro, Injection

    mda mb 435s cell line  (ATCC)


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    ATCC mda mb 435s cell line
    Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, <t>MDA-MB-231,</t> BT-549, and <t>MDA-MB-435S</t> by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.
    Mda Mb 435s Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold"

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S66723

    Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, MDA-MB-231, BT-549, and MDA-MB-435S by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.
    Figure Legend Snippet: Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, MDA-MB-231, BT-549, and MDA-MB-435S by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.

    Techniques Used: Flow Cytometry

    The reversion of the malignant phenotype in the morphology of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) AFM images of collagen I, Matrigel, and RADA16 peptide nanofiber scaffolds. Scale bars represent 500 nm. ( B ) Light microscope images (upper) and F-actin and nuclear fluorescence images (lower) of cells encapsulated in collagen I, Matrigel, and RADA16 peptide scaffolds. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (upper) and 50 μm (lower). ( C ) F-actin and nuclear fluorescence images (upper) and light microscope images (lower) of most common morphology of polarized cell colonies formed in RADA16 scaffold. Scale bars represent 25 μm. ( D ) Encapsulation of cells in different scaffolds using calcein-AM staining for the living cells. Scale bar represents 500 μm. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: AFM, atomic force microscopy; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .
    Figure Legend Snippet: The reversion of the malignant phenotype in the morphology of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) AFM images of collagen I, Matrigel, and RADA16 peptide nanofiber scaffolds. Scale bars represent 500 nm. ( B ) Light microscope images (upper) and F-actin and nuclear fluorescence images (lower) of cells encapsulated in collagen I, Matrigel, and RADA16 peptide scaffolds. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (upper) and 50 μm (lower). ( C ) F-actin and nuclear fluorescence images (upper) and light microscope images (lower) of most common morphology of polarized cell colonies formed in RADA16 scaffold. Scale bars represent 25 μm. ( D ) Encapsulation of cells in different scaffolds using calcein-AM staining for the living cells. Scale bar represents 500 μm. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: AFM, atomic force microscopy; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Techniques Used: Light Microscopy, Fluorescence, Staining, Microscopy

    The reversion of the malignant phenotype in the proliferation and migration potential of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) Cell density in different scaffolds calculated from DNA measurement after 3-day, 5-day, 7-day, and 9-day culture. ( B ) Percentage of BrdU labeling in cells grown in different scaffolds expressed as the BrdU labeling index from three separate experiments (about 200 cells/experiment) on days 3, 5, 7, and 9. ( C ) Migration potential of MDA-MB-435S cells in Matrigel, collagen I, and RADA16 scaffolds; * P <0.05; ** P <0.001; *** P <0.0001. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: BrdU, 5-bromo-2′-deoxyuridine; C, collagen; M, Matrigel; R, RADA16; SEM, standard error of the mean; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .
    Figure Legend Snippet: The reversion of the malignant phenotype in the proliferation and migration potential of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) Cell density in different scaffolds calculated from DNA measurement after 3-day, 5-day, 7-day, and 9-day culture. ( B ) Percentage of BrdU labeling in cells grown in different scaffolds expressed as the BrdU labeling index from three separate experiments (about 200 cells/experiment) on days 3, 5, 7, and 9. ( C ) Migration potential of MDA-MB-435S cells in Matrigel, collagen I, and RADA16 scaffolds; * P <0.05; ** P <0.001; *** P <0.0001. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: BrdU, 5-bromo-2′-deoxyuridine; C, collagen; M, Matrigel; R, RADA16; SEM, standard error of the mean; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Techniques Used: Migration, Labeling

    Cellular characteristics and the localization and expression of signaling proteins in RADA16 cells colonies. Notes: ( A ) Hematoxylin staining of cryostat sections of the Matrigel and RADA16 scaffolds show lumen formation only in RADA16 scaffold. Scale bar represents 25 μm. ( B ) The nuclei of cell colonies stained with DAPI show lumen formation in RADA16 and irregular organization in Matrigel. Scale bar represents 25 μm. ( C ) Immunolocalization of β-catenin (green) with DAPI stained nuclei (blue) in RADA16 and Matrigel scaffolds. The β-catenin is diffusely localized in the cytoplasm, nucleus, and some cell–cell contacts within colonies formed in Matrigel but forms intense localization in the cell–cell junctions in colonies formed in RADA16, indicating similar β-catenin distribution like that of nonmalignant S-1 cells. Scale bar represents 25 μm. ( D ) Western blot analysis of the indicated proteins β-catenin and ICAM-1 of MDA-MB-435S cells in different three-dimensional cultures at 6 days and 9 days. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: 6 d, 6-day cultures; 9 d, 9-day cultures; C, collagen; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; M, Matrigel; R, RADA16; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .
    Figure Legend Snippet: Cellular characteristics and the localization and expression of signaling proteins in RADA16 cells colonies. Notes: ( A ) Hematoxylin staining of cryostat sections of the Matrigel and RADA16 scaffolds show lumen formation only in RADA16 scaffold. Scale bar represents 25 μm. ( B ) The nuclei of cell colonies stained with DAPI show lumen formation in RADA16 and irregular organization in Matrigel. Scale bar represents 25 μm. ( C ) Immunolocalization of β-catenin (green) with DAPI stained nuclei (blue) in RADA16 and Matrigel scaffolds. The β-catenin is diffusely localized in the cytoplasm, nucleus, and some cell–cell contacts within colonies formed in Matrigel but forms intense localization in the cell–cell junctions in colonies formed in RADA16, indicating similar β-catenin distribution like that of nonmalignant S-1 cells. Scale bar represents 25 μm. ( D ) Western blot analysis of the indicated proteins β-catenin and ICAM-1 of MDA-MB-435S cells in different three-dimensional cultures at 6 days and 9 days. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: 6 d, 6-day cultures; 9 d, 9-day cultures; C, collagen; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; M, Matrigel; R, RADA16; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Techniques Used: Expressing, Staining, Western Blot

    The reversion phenotype of MDA-MB-435S cells was inhibited by blocking NF-κB signaling by PDTC. Notes: ( A ) Western blot analysis of ICAM-1 of MDA-MB-435S cells in different culture groups. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. The expression of ICAM-1 of cells in RADA16 was significantly downregulated by PDTC (* P <0.05). ( B ) Light microscope images and F-actin and nuclear fluorescence images of cells in PDTC-treated RADA16 group. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (left) and 50 μm (right). Abbreviations: 2D, two dimensional group; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; PDTC, pyrrolidine dithiocarbamate; R, RADA16 group; Rp, RADA16 + PDTC group; RADA16, COCH 3 -RADARADAR-ADARADA-CONH 2 .
    Figure Legend Snippet: The reversion phenotype of MDA-MB-435S cells was inhibited by blocking NF-κB signaling by PDTC. Notes: ( A ) Western blot analysis of ICAM-1 of MDA-MB-435S cells in different culture groups. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. The expression of ICAM-1 of cells in RADA16 was significantly downregulated by PDTC (* P <0.05). ( B ) Light microscope images and F-actin and nuclear fluorescence images of cells in PDTC-treated RADA16 group. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (left) and 50 μm (right). Abbreviations: 2D, two dimensional group; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; PDTC, pyrrolidine dithiocarbamate; R, RADA16 group; Rp, RADA16 + PDTC group; RADA16, COCH 3 -RADARADAR-ADARADA-CONH 2 .

    Techniques Used: Blocking Assay, Western Blot, Expressing, Light Microscopy, Fluorescence, Staining

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    ATCC mda mb 435 cell lines
    ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an <t>MDA-MB-435</t> orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).
    Mda Mb 435 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC cell line mda mb 435s
    ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an <t>MDA-MB-435</t> orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).
    Cell Line Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human melanoma cell line mda mb 435s
    ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an <t>MDA-MB-435</t> orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).
    Human Melanoma Cell Line Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC breast cancer cell line mda mb 435
    ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an <t>MDA-MB-435</t> orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).
    Breast Cancer Cell Line Mda Mb 435, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human breast cancer cells mda mb 435 melanoma cell line meoh methanol mgc
    ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an <t>MDA-MB-435</t> orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).
    Human Breast Cancer Cells Mda Mb 435 Melanoma Cell Line Meoh Methanol Mgc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC ductal carcinoma cell line mda mb 435s
    Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, <t>MDA-MB</t> <t>435S)</t> were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.
    Ductal Carcinoma Cell Line Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mda mb 435s cell line
    Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, <t>MDA-MB-231,</t> BT-549, and <t>MDA-MB-435S</t> by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.
    Mda Mb 435s Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an MDA-MB-435 orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).

    Journal: PLoS ONE

    Article Title: Non-Invasive Imaging of Tumors by Monitoring Autotaxin Activity Using an Enzyme-Activated Near-Infrared Fluorogenic Substrate

    doi: 10.1371/journal.pone.0079065

    Figure Lengend Snippet: ( a ) A mouse bearing an MDA-MB-231 orthotopic tumor (arrow) was injected with AR-2 by tail vein injection and NIRF was imaged. ( b ) AR-2 tumor-bearing mice similar to (a), or bearing an MDA-MB-435 orthotopic tumor were imaged and in vivo tumor fluorescence was determined (n = 11 for MDA-MB-231 or n = 3 for MDA-MB-435). Tumors were then excised and ATX levels were measured in tumor homogenates by ELISA. The data were plotted as tumor fluorescence versus ATX mass and fit using linear regression analysis. The slope of the resulting line demonstrates significant correlation (p<0.0001).

    Article Snippet: COS-7, MDA-MB-231, and MDA-MB-435 cell lines were purchased from ATCC (Manassas, VA).

    Techniques: Injection, In Vivo, Fluorescence, Enzyme-linked Immunosorbent Assay

    Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, MDA-MB 435S) were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.

    Journal: Microbial Cell Factories

    Article Title: High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

    doi: 10.1186/1475-2859-12-97

    Figure Lengend Snippet: Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, MDA-MB 435S) were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.

    Article Snippet: For binding and uptake studies, three different adherent human tumor cell lines were used: the epidermoid carcinoma cell line A431 (ATCC® Number: CRL-1555), the squamous cell carcinoma cell line FaDu (ATCC® Number: HTB-43), the ductal carcinoma cell line MDA-MB 435S (ATCC® Number: HTB-129).

    Techniques: Expressing, Binding Assay, SDS Page, Western Blot, Incubation, In Vitro, Injection

    Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, MDA-MB-231, BT-549, and MDA-MB-435S by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.

    Journal: International Journal of Nanomedicine

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    doi: 10.2147/IJN.S66723

    Figure Lengend Snippet: Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, MDA-MB-231, BT-549, and MDA-MB-435S by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.

    Article Snippet: The MDA-MB-435S cell line was obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Flow Cytometry

    The reversion of the malignant phenotype in the morphology of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) AFM images of collagen I, Matrigel, and RADA16 peptide nanofiber scaffolds. Scale bars represent 500 nm. ( B ) Light microscope images (upper) and F-actin and nuclear fluorescence images (lower) of cells encapsulated in collagen I, Matrigel, and RADA16 peptide scaffolds. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (upper) and 50 μm (lower). ( C ) F-actin and nuclear fluorescence images (upper) and light microscope images (lower) of most common morphology of polarized cell colonies formed in RADA16 scaffold. Scale bars represent 25 μm. ( D ) Encapsulation of cells in different scaffolds using calcein-AM staining for the living cells. Scale bar represents 500 μm. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: AFM, atomic force microscopy; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Journal: International Journal of Nanomedicine

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    doi: 10.2147/IJN.S66723

    Figure Lengend Snippet: The reversion of the malignant phenotype in the morphology of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) AFM images of collagen I, Matrigel, and RADA16 peptide nanofiber scaffolds. Scale bars represent 500 nm. ( B ) Light microscope images (upper) and F-actin and nuclear fluorescence images (lower) of cells encapsulated in collagen I, Matrigel, and RADA16 peptide scaffolds. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (upper) and 50 μm (lower). ( C ) F-actin and nuclear fluorescence images (upper) and light microscope images (lower) of most common morphology of polarized cell colonies formed in RADA16 scaffold. Scale bars represent 25 μm. ( D ) Encapsulation of cells in different scaffolds using calcein-AM staining for the living cells. Scale bar represents 500 μm. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: AFM, atomic force microscopy; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Article Snippet: The MDA-MB-435S cell line was obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Light Microscopy, Fluorescence, Staining, Microscopy

    The reversion of the malignant phenotype in the proliferation and migration potential of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) Cell density in different scaffolds calculated from DNA measurement after 3-day, 5-day, 7-day, and 9-day culture. ( B ) Percentage of BrdU labeling in cells grown in different scaffolds expressed as the BrdU labeling index from three separate experiments (about 200 cells/experiment) on days 3, 5, 7, and 9. ( C ) Migration potential of MDA-MB-435S cells in Matrigel, collagen I, and RADA16 scaffolds; * P <0.05; ** P <0.001; *** P <0.0001. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: BrdU, 5-bromo-2′-deoxyuridine; C, collagen; M, Matrigel; R, RADA16; SEM, standard error of the mean; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Journal: International Journal of Nanomedicine

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    doi: 10.2147/IJN.S66723

    Figure Lengend Snippet: The reversion of the malignant phenotype in the proliferation and migration potential of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) Cell density in different scaffolds calculated from DNA measurement after 3-day, 5-day, 7-day, and 9-day culture. ( B ) Percentage of BrdU labeling in cells grown in different scaffolds expressed as the BrdU labeling index from three separate experiments (about 200 cells/experiment) on days 3, 5, 7, and 9. ( C ) Migration potential of MDA-MB-435S cells in Matrigel, collagen I, and RADA16 scaffolds; * P <0.05; ** P <0.001; *** P <0.0001. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: BrdU, 5-bromo-2′-deoxyuridine; C, collagen; M, Matrigel; R, RADA16; SEM, standard error of the mean; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Article Snippet: The MDA-MB-435S cell line was obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Migration, Labeling

    Cellular characteristics and the localization and expression of signaling proteins in RADA16 cells colonies. Notes: ( A ) Hematoxylin staining of cryostat sections of the Matrigel and RADA16 scaffolds show lumen formation only in RADA16 scaffold. Scale bar represents 25 μm. ( B ) The nuclei of cell colonies stained with DAPI show lumen formation in RADA16 and irregular organization in Matrigel. Scale bar represents 25 μm. ( C ) Immunolocalization of β-catenin (green) with DAPI stained nuclei (blue) in RADA16 and Matrigel scaffolds. The β-catenin is diffusely localized in the cytoplasm, nucleus, and some cell–cell contacts within colonies formed in Matrigel but forms intense localization in the cell–cell junctions in colonies formed in RADA16, indicating similar β-catenin distribution like that of nonmalignant S-1 cells. Scale bar represents 25 μm. ( D ) Western blot analysis of the indicated proteins β-catenin and ICAM-1 of MDA-MB-435S cells in different three-dimensional cultures at 6 days and 9 days. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: 6 d, 6-day cultures; 9 d, 9-day cultures; C, collagen; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; M, Matrigel; R, RADA16; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Journal: International Journal of Nanomedicine

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    doi: 10.2147/IJN.S66723

    Figure Lengend Snippet: Cellular characteristics and the localization and expression of signaling proteins in RADA16 cells colonies. Notes: ( A ) Hematoxylin staining of cryostat sections of the Matrigel and RADA16 scaffolds show lumen formation only in RADA16 scaffold. Scale bar represents 25 μm. ( B ) The nuclei of cell colonies stained with DAPI show lumen formation in RADA16 and irregular organization in Matrigel. Scale bar represents 25 μm. ( C ) Immunolocalization of β-catenin (green) with DAPI stained nuclei (blue) in RADA16 and Matrigel scaffolds. The β-catenin is diffusely localized in the cytoplasm, nucleus, and some cell–cell contacts within colonies formed in Matrigel but forms intense localization in the cell–cell junctions in colonies formed in RADA16, indicating similar β-catenin distribution like that of nonmalignant S-1 cells. Scale bar represents 25 μm. ( D ) Western blot analysis of the indicated proteins β-catenin and ICAM-1 of MDA-MB-435S cells in different three-dimensional cultures at 6 days and 9 days. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: 6 d, 6-day cultures; 9 d, 9-day cultures; C, collagen; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; M, Matrigel; R, RADA16; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Article Snippet: The MDA-MB-435S cell line was obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Expressing, Staining, Western Blot

    The reversion phenotype of MDA-MB-435S cells was inhibited by blocking NF-κB signaling by PDTC. Notes: ( A ) Western blot analysis of ICAM-1 of MDA-MB-435S cells in different culture groups. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. The expression of ICAM-1 of cells in RADA16 was significantly downregulated by PDTC (* P <0.05). ( B ) Light microscope images and F-actin and nuclear fluorescence images of cells in PDTC-treated RADA16 group. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (left) and 50 μm (right). Abbreviations: 2D, two dimensional group; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; PDTC, pyrrolidine dithiocarbamate; R, RADA16 group; Rp, RADA16 + PDTC group; RADA16, COCH 3 -RADARADAR-ADARADA-CONH 2 .

    Journal: International Journal of Nanomedicine

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    doi: 10.2147/IJN.S66723

    Figure Lengend Snippet: The reversion phenotype of MDA-MB-435S cells was inhibited by blocking NF-κB signaling by PDTC. Notes: ( A ) Western blot analysis of ICAM-1 of MDA-MB-435S cells in different culture groups. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. The expression of ICAM-1 of cells in RADA16 was significantly downregulated by PDTC (* P <0.05). ( B ) Light microscope images and F-actin and nuclear fluorescence images of cells in PDTC-treated RADA16 group. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (left) and 50 μm (right). Abbreviations: 2D, two dimensional group; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; PDTC, pyrrolidine dithiocarbamate; R, RADA16 group; Rp, RADA16 + PDTC group; RADA16, COCH 3 -RADARADAR-ADARADA-CONH 2 .

    Article Snippet: The MDA-MB-435S cell line was obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Blocking Assay, Western Blot, Expressing, Light Microscopy, Fluorescence, Staining