Structured Review

OriGene mcl 1 open reading frame
The regulation of <t>Mcl-1</t> stability by ERK1/2 and the sensitivity of cells to U0126. (A) The levels of phospho-ERK1/2 (p-ERK1/2) and total ERK1/2 (t-ERK1/2) as determined by Western blot. A representative example of three blots is shown. (B) Drug sensitivity
Mcl 1 Open Reading Frame, supplied by OriGene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mcl 1 open reading frame/product/OriGene
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mcl 1 open reading frame - by Bioz Stars, 2020-09
86/100 stars

Related Products / Commonly Used Together

sirna
mcl-1
pegfp-n1 eukaryotic expression vector

Images

1) Product Images from "Overexpression of Mcl-1 Confers Multidrug Resistance, Whereas Topoisomerase IIβ Downregulation Introduces Mitoxantrone-Specific Drug Resistance in Acute Myeloid Leukemia"

Article Title: Overexpression of Mcl-1 Confers Multidrug Resistance, Whereas Topoisomerase IIβ Downregulation Introduces Mitoxantrone-Specific Drug Resistance in Acute Myeloid Leukemia

Journal: Molecular Pharmacology

doi: 10.1124/mol.113.086140

The regulation of Mcl-1 stability by ERK1/2 and the sensitivity of cells to U0126. (A) The levels of phospho-ERK1/2 (p-ERK1/2) and total ERK1/2 (t-ERK1/2) as determined by Western blot. A representative example of three blots is shown. (B) Drug sensitivity
Figure Legend Snippet: The regulation of Mcl-1 stability by ERK1/2 and the sensitivity of cells to U0126. (A) The levels of phospho-ERK1/2 (p-ERK1/2) and total ERK1/2 (t-ERK1/2) as determined by Western blot. A representative example of three blots is shown. (B) Drug sensitivity

Techniques Used: Western Blot

The levels of Mcl-1 among HL60, HL60/MX2, and HL60/MX2/CXL017 cells and the impact of Mcl-1 downregulation on cell survival. (A) Western blot of protein levels of Mcl-1 in various cell lines. Densitometry was performed and normalized to β -actin
Figure Legend Snippet: The levels of Mcl-1 among HL60, HL60/MX2, and HL60/MX2/CXL017 cells and the impact of Mcl-1 downregulation on cell survival. (A) Western blot of protein levels of Mcl-1 in various cell lines. Densitometry was performed and normalized to β -actin

Techniques Used: Western Blot

The impact of Mcl-1 upregulation on drug sensitivity. (A) Western blot of Mcl-1 levels in HL60 cells 24 hours after transfection with Mcl-1 open reading frame. (B–D) Drug sensitivity of HL60/Mcl-1 (♦) cells to mitoxantrone (B), ABT-737
Figure Legend Snippet: The impact of Mcl-1 upregulation on drug sensitivity. (A) Western blot of Mcl-1 levels in HL60 cells 24 hours after transfection with Mcl-1 open reading frame. (B–D) Drug sensitivity of HL60/Mcl-1 (♦) cells to mitoxantrone (B), ABT-737

Techniques Used: Western Blot, Transfection

The regulation of Mcl-1 protein among HL60, HL60/MX2, and HL60/MX2/CXL017 cells. (A) Western Blot of Mcl-1 translation after addition of the proteasome inhibitor MG-132 for the indicated time points. Densitometry was performed and normalized to β
Figure Legend Snippet: The regulation of Mcl-1 protein among HL60, HL60/MX2, and HL60/MX2/CXL017 cells. (A) Western Blot of Mcl-1 translation after addition of the proteasome inhibitor MG-132 for the indicated time points. Densitometry was performed and normalized to β

Techniques Used: Western Blot

Related Articles

Transfection:

Article Title: Overexpression of Mcl-1 Confers Multidrug Resistance, Whereas Topoisomerase IIβ Downregulation Introduces Mitoxantrone-Specific Drug Resistance in Acute Myeloid Leukemia
Article Snippet: .. Cells were either transfected with 12.5 nM small interfering (siRNA) (based on 2.5 ml resuspension volume) from Life Technologies or 5 µ g plasmid containing the Mcl-1 open reading frame (SC315538) from OriGene (Rockville, MD). .. Downregulation was assessed after 3 hours and upregulation was assessed after 24 hours via Western blotting.

Plasmid Preparation:

Article Title: Overexpression of Mcl-1 Confers Multidrug Resistance, Whereas Topoisomerase IIβ Downregulation Introduces Mitoxantrone-Specific Drug Resistance in Acute Myeloid Leukemia
Article Snippet: .. Cells were either transfected with 12.5 nM small interfering (siRNA) (based on 2.5 ml resuspension volume) from Life Technologies or 5 µ g plasmid containing the Mcl-1 open reading frame (SC315538) from OriGene (Rockville, MD). .. Downregulation was assessed after 3 hours and upregulation was assessed after 24 hours via Western blotting.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    OriGene mcl 1 open reading frame
    The regulation of <t>Mcl-1</t> stability by ERK1/2 and the sensitivity of cells to U0126. (A) The levels of phospho-ERK1/2 (p-ERK1/2) and total ERK1/2 (t-ERK1/2) as determined by Western blot. A representative example of three blots is shown. (B) Drug sensitivity
    Mcl 1 Open Reading Frame, supplied by OriGene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mcl 1 open reading frame/product/OriGene
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mcl 1 open reading frame - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    85
    OriGene human orf constructs
    The regulation of <t>Mcl-1</t> stability by ERK1/2 and the sensitivity of cells to U0126. (A) The levels of phospho-ERK1/2 (p-ERK1/2) and total ERK1/2 (t-ERK1/2) as determined by Western blot. A representative example of three blots is shown. (B) Drug sensitivity
    Human Orf Constructs, supplied by OriGene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human orf constructs/product/OriGene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human orf constructs - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    The regulation of Mcl-1 stability by ERK1/2 and the sensitivity of cells to U0126. (A) The levels of phospho-ERK1/2 (p-ERK1/2) and total ERK1/2 (t-ERK1/2) as determined by Western blot. A representative example of three blots is shown. (B) Drug sensitivity

    Journal: Molecular Pharmacology

    Article Title: Overexpression of Mcl-1 Confers Multidrug Resistance, Whereas Topoisomerase IIβ Downregulation Introduces Mitoxantrone-Specific Drug Resistance in Acute Myeloid Leukemia

    doi: 10.1124/mol.113.086140

    Figure Lengend Snippet: The regulation of Mcl-1 stability by ERK1/2 and the sensitivity of cells to U0126. (A) The levels of phospho-ERK1/2 (p-ERK1/2) and total ERK1/2 (t-ERK1/2) as determined by Western blot. A representative example of three blots is shown. (B) Drug sensitivity

    Article Snippet: Cells were either transfected with 12.5 nM small interfering (siRNA) (based on 2.5 ml resuspension volume) from Life Technologies or 5 µ g plasmid containing the Mcl-1 open reading frame (SC315538) from OriGene (Rockville, MD).

    Techniques: Western Blot

    The levels of Mcl-1 among HL60, HL60/MX2, and HL60/MX2/CXL017 cells and the impact of Mcl-1 downregulation on cell survival. (A) Western blot of protein levels of Mcl-1 in various cell lines. Densitometry was performed and normalized to β -actin

    Journal: Molecular Pharmacology

    Article Title: Overexpression of Mcl-1 Confers Multidrug Resistance, Whereas Topoisomerase IIβ Downregulation Introduces Mitoxantrone-Specific Drug Resistance in Acute Myeloid Leukemia

    doi: 10.1124/mol.113.086140

    Figure Lengend Snippet: The levels of Mcl-1 among HL60, HL60/MX2, and HL60/MX2/CXL017 cells and the impact of Mcl-1 downregulation on cell survival. (A) Western blot of protein levels of Mcl-1 in various cell lines. Densitometry was performed and normalized to β -actin

    Article Snippet: Cells were either transfected with 12.5 nM small interfering (siRNA) (based on 2.5 ml resuspension volume) from Life Technologies or 5 µ g plasmid containing the Mcl-1 open reading frame (SC315538) from OriGene (Rockville, MD).

    Techniques: Western Blot

    The impact of Mcl-1 upregulation on drug sensitivity. (A) Western blot of Mcl-1 levels in HL60 cells 24 hours after transfection with Mcl-1 open reading frame. (B–D) Drug sensitivity of HL60/Mcl-1 (♦) cells to mitoxantrone (B), ABT-737

    Journal: Molecular Pharmacology

    Article Title: Overexpression of Mcl-1 Confers Multidrug Resistance, Whereas Topoisomerase IIβ Downregulation Introduces Mitoxantrone-Specific Drug Resistance in Acute Myeloid Leukemia

    doi: 10.1124/mol.113.086140

    Figure Lengend Snippet: The impact of Mcl-1 upregulation on drug sensitivity. (A) Western blot of Mcl-1 levels in HL60 cells 24 hours after transfection with Mcl-1 open reading frame. (B–D) Drug sensitivity of HL60/Mcl-1 (♦) cells to mitoxantrone (B), ABT-737

    Article Snippet: Cells were either transfected with 12.5 nM small interfering (siRNA) (based on 2.5 ml resuspension volume) from Life Technologies or 5 µ g plasmid containing the Mcl-1 open reading frame (SC315538) from OriGene (Rockville, MD).

    Techniques: Western Blot, Transfection

    The regulation of Mcl-1 protein among HL60, HL60/MX2, and HL60/MX2/CXL017 cells. (A) Western Blot of Mcl-1 translation after addition of the proteasome inhibitor MG-132 for the indicated time points. Densitometry was performed and normalized to β

    Journal: Molecular Pharmacology

    Article Title: Overexpression of Mcl-1 Confers Multidrug Resistance, Whereas Topoisomerase IIβ Downregulation Introduces Mitoxantrone-Specific Drug Resistance in Acute Myeloid Leukemia

    doi: 10.1124/mol.113.086140

    Figure Lengend Snippet: The regulation of Mcl-1 protein among HL60, HL60/MX2, and HL60/MX2/CXL017 cells. (A) Western Blot of Mcl-1 translation after addition of the proteasome inhibitor MG-132 for the indicated time points. Densitometry was performed and normalized to β

    Article Snippet: Cells were either transfected with 12.5 nM small interfering (siRNA) (based on 2.5 ml resuspension volume) from Life Technologies or 5 µ g plasmid containing the Mcl-1 open reading frame (SC315538) from OriGene (Rockville, MD).

    Techniques: Western Blot