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Pharmingen mck 1
Mck 1, supplied by Pharmingen, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mck 1/product/Pharmingen
Average 88 stars, based on 2 article reviews
Price from $9.99 to $1999.99
mck 1 - by Bioz Stars, 2020-08
88/100 stars

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Related Articles

Rnase Protection Assay:

Article Title: Regulation of ITAM-positive receptors: role of IL-12 and IL-18
Article Snippet: .. The multiprobe RNAse Protection Assay (RPA) was performed using the mck-1 or mck-5 template set (PharMingen, San Diego, CA). .. Total cellular RNA was extracted using Trizol (Life Technologies, Gaithersburg, MD), and 1-5 μg total mRNA was hybridized with a 33-P UTP-labeled RNA probe (1 × 106 cpm/sample) prepared according to the manufacturer's directions (PharMingen, La Jolla, CA) using the PharMingen RiboQuant In Vitro Transcription kit.

Recombinase Polymerase Amplification:

Article Title: Regulation of ITAM-positive receptors: role of IL-12 and IL-18
Article Snippet: .. The multiprobe RNAse Protection Assay (RPA) was performed using the mck-1 or mck-5 template set (PharMingen, San Diego, CA). .. Total cellular RNA was extracted using Trizol (Life Technologies, Gaithersburg, MD), and 1-5 μg total mRNA was hybridized with a 33-P UTP-labeled RNA probe (1 × 106 cpm/sample) prepared according to the manufacturer's directions (PharMingen, La Jolla, CA) using the PharMingen RiboQuant In Vitro Transcription kit.

In Vitro:

Article Title: Murine metal-induced systemic autoimmunity: baseline and stimulated cytokine mRNA expression in genetically susceptible and resistant strains
Article Snippet: .. Briefly, multiprobes incorporating [ α 32 P]UTP were transcribed from the template set mCK-1 (Cat. #45001P, Pharmingen) using T7 polymerase from an in vitro transcription kit (Cat. #45004K, Pharmingen). .. Hybridization and digestion of unprotected probes was done with an RPA kit (Cat# 45004K, Pharmingen).

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  • 80
    Pharmingen mck 1 kit for interleukin il 4
    Time-course of IFN-γ gene expression in polyphenolic antioxidant-treated, antigen-IgE activated mast cells. Levels of cytokine messages of antioxidant-treated mast cells were determined by RNase protection assay with RiboQuant™, <t>mCK-1</t> kit from PharMingen (for <t>IL-4,</t> IL-5, IL-10, IL-13, IL-15, IL-9, IL-2, IL-6, IFN-γ). Similar kinetics for various cytokine messages was noted, and IFN-γ was shown. Mast cells were cultured in T-75 flask at 1 × 10 6 /ml with 2 µg/ml IgE overnight in the presence of different concentrations of rutin/CGA overnight. Cells were washed twice with Tyrode's buffer, resuspended in conditioned medium, and challenged at 37° with DNP-BSA at 100 ng/ml at different time-points. Cells were then pelleted, total RNA extracted and cytokine messages calibrated with housekeeping gene as standard by a PhosphoImager (Molecular Dynamics). Units of expression were computed by dividing the intensity of the individual band over that of L32 gene in each respective lane. Mean and SEM of densitometric readings of two similarly performed experiments are presented.
    Mck 1 Kit For Interleukin Il 4, supplied by Pharmingen, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mck 1 kit for interleukin il 4/product/Pharmingen
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mck 1 kit for interleukin il 4 - by Bioz Stars, 2020-08
    80/100 stars
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    85
    Pharmingen riboquant multiprobe rpa kit
    Representative panels and bar graphs showing the results of <t>RPA.</t> Ten micrograms of total RNA from each heart graft or normal heart was analyzed, using <t>multiprobe</t> RPA kit mCK-1 (containing DNA templates for IL-4, IL-5, IL-10, IL-13, IL-15, IL-9, IL-2, IL-6, IFN-γ, L32, and GAPDH). A: Representative gel images analyzed by mCK-1. B: Bar graph showing the results of densitometric analysis for IL-4 gene expression in the allografts ( n = 3 for each group). IL-4 gene expression decreased significantly in the allografts in B7-2 −/− and B7-1/B7-2 −/− recipients compared with wild-type recipients. C: Bar graph showing the results of densitometric analysis for IFN-γ gene expression in the allografts ( n = 3 for each group). IFN-γ gene expression was comparable in all allograft groups.
    Riboquant Multiprobe Rpa Kit, supplied by Pharmingen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/riboquant multiprobe rpa kit/product/Pharmingen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    riboquant multiprobe rpa kit - by Bioz Stars, 2020-08
    85/100 stars
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    85
    Pharmingen mck 2b
    Representative panels of RPA from transplanted hearts. Fifteen micrograms of total RNA from normal non-transplanted hearts, or from hearts explanted at 0 hour, 4 hour, 24 hour, 3 days, or 7 days after transplantation were analyzed using the multiprobe RPA kits <t>mCK-3b</t> (containing DNA templates for TNF-β, LT-β, TNF-α, IL-6, IFN-γ, IFN-β, TGF-β1, TGF-β2, TGF-β3, MIF, L32, and GAPDH) and <t>mCK-2b</t> (containing DNA templates for IL-12p35, IL-12p40, IL-10, IL-1α, IL-1β, IL-1Ra, IL-18, IL-6, IFN-γ, MIF, L32, and GAPDH). The lanes on the far left show the undigested labeled probes that serve as size references, and the lanes on the far right show the results of RPA using a control RNA sample that was supplied by a manufacturer of the kit. Left panel: Representative gel images of B/6 isografts experiment analyzed by mCK-3b. Right panel: Representative gel images of BALB/c to B/6 allografts experiment analyzed by mCK-3b.
    Mck 2b, supplied by Pharmingen, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mck 2b/product/Pharmingen
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mck 2b - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    85
    Pharmingen rna protection assay
    <t>Cytokine</t> profile of splenocytes from Friend virus-infected mice after ODN treatment. (a) Levels of cytokine transcripts from splenocytes derived from Friend virus-infected CpG-treated mice (gray bars) and control ODN-inoculated mice (black bars) were compared by <t>RNA</t> protection assays. Cells were taken at 4 weeks postinfection and stimulated with phorbol-12-myristate13-acetate/ionomycin for 5 h. Band densities are expressed as percentages of specific cytokine band density of that of an internal housekeeping transcript band (GAPDH). Since CpG-ODN promote Th1 responses, only the typical Th1/Th2-type cytokines IL-2, IL-4, and IFN-γ are shown. The differences in mRNA levels between the groups of treated and untreated mice were statistically significant by Mann-Whitney test for IL-2 ( P
    Rna Protection Assay, supplied by Pharmingen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna protection assay/product/Pharmingen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rna protection assay - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Time-course of IFN-γ gene expression in polyphenolic antioxidant-treated, antigen-IgE activated mast cells. Levels of cytokine messages of antioxidant-treated mast cells were determined by RNase protection assay with RiboQuant™, mCK-1 kit from PharMingen (for IL-4, IL-5, IL-10, IL-13, IL-15, IL-9, IL-2, IL-6, IFN-γ). Similar kinetics for various cytokine messages was noted, and IFN-γ was shown. Mast cells were cultured in T-75 flask at 1 × 10 6 /ml with 2 µg/ml IgE overnight in the presence of different concentrations of rutin/CGA overnight. Cells were washed twice with Tyrode's buffer, resuspended in conditioned medium, and challenged at 37° with DNP-BSA at 100 ng/ml at different time-points. Cells were then pelleted, total RNA extracted and cytokine messages calibrated with housekeeping gene as standard by a PhosphoImager (Molecular Dynamics). Units of expression were computed by dividing the intensity of the individual band over that of L32 gene in each respective lane. Mean and SEM of densitometric readings of two similarly performed experiments are presented.

    Journal: Immunology

    Article Title: Naturally occurring polyphenolic antioxidants modulate IgE-mediated mast cell activation

    doi: 10.1046/j.1365-2567.2000.00045.x

    Figure Lengend Snippet: Time-course of IFN-γ gene expression in polyphenolic antioxidant-treated, antigen-IgE activated mast cells. Levels of cytokine messages of antioxidant-treated mast cells were determined by RNase protection assay with RiboQuant™, mCK-1 kit from PharMingen (for IL-4, IL-5, IL-10, IL-13, IL-15, IL-9, IL-2, IL-6, IFN-γ). Similar kinetics for various cytokine messages was noted, and IFN-γ was shown. Mast cells were cultured in T-75 flask at 1 × 10 6 /ml with 2 µg/ml IgE overnight in the presence of different concentrations of rutin/CGA overnight. Cells were washed twice with Tyrode's buffer, resuspended in conditioned medium, and challenged at 37° with DNP-BSA at 100 ng/ml at different time-points. Cells were then pelleted, total RNA extracted and cytokine messages calibrated with housekeeping gene as standard by a PhosphoImager (Molecular Dynamics). Units of expression were computed by dividing the intensity of the individual band over that of L32 gene in each respective lane. Mean and SEM of densitometric readings of two similarly performed experiments are presented.

    Article Snippet: Levels of cytokine messages of polyphenol-treated mast cells were determined by RNase protection assay (RPA) with RiboQuant™, mCK-1 kit (for interleukin (IL)-4, IL-5, IL-10, IL-13, IL-15, IL-9, IL-2, IL-6, interferon-γ (IFN-γ)), and mCK-3 kit (for tumour necrosis factor-β (TNF-β), leukotriene-β (LTβ), TNF-α, IL-6, IFN-γ, transforming growth factor-β1 (TGF-β1) and TGF-β2) from PharMingen (San Diego, CA).

    Techniques: Expressing, Rnase Protection Assay, Cell Culture

    Representative panels and bar graphs showing the results of RPA. Ten micrograms of total RNA from each heart graft or normal heart was analyzed, using multiprobe RPA kit mCK-1 (containing DNA templates for IL-4, IL-5, IL-10, IL-13, IL-15, IL-9, IL-2, IL-6, IFN-γ, L32, and GAPDH). A: Representative gel images analyzed by mCK-1. B: Bar graph showing the results of densitometric analysis for IL-4 gene expression in the allografts ( n = 3 for each group). IL-4 gene expression decreased significantly in the allografts in B7-2 −/− and B7-1/B7-2 −/− recipients compared with wild-type recipients. C: Bar graph showing the results of densitometric analysis for IFN-γ gene expression in the allografts ( n = 3 for each group). IFN-γ gene expression was comparable in all allograft groups.

    Journal: The American Journal of Pathology

    Article Title: Association of B7-1 Co-Stimulation with the Development of Graft Arterial Disease

    doi:

    Figure Lengend Snippet: Representative panels and bar graphs showing the results of RPA. Ten micrograms of total RNA from each heart graft or normal heart was analyzed, using multiprobe RPA kit mCK-1 (containing DNA templates for IL-4, IL-5, IL-10, IL-13, IL-15, IL-9, IL-2, IL-6, IFN-γ, L32, and GAPDH). A: Representative gel images analyzed by mCK-1. B: Bar graph showing the results of densitometric analysis for IL-4 gene expression in the allografts ( n = 3 for each group). IL-4 gene expression decreased significantly in the allografts in B7-2 −/− and B7-1/B7-2 −/− recipients compared with wild-type recipients. C: Bar graph showing the results of densitometric analysis for IFN-γ gene expression in the allografts ( n = 3 for each group). IFN-γ gene expression was comparable in all allograft groups.

    Article Snippet: Purified anti-CD45R/B220 antibody (RA3-6B2), purified anti-CD11b antibody (M1/70), purified anti-CD16/CD32 antibody (Fc-block; clone 2.4G2), purified anti-CD28 antibody (37.51), purified anti-CD4 antibody (RM4-5), purified anti-CD8 antibody (53–6.7), fluorescein isothiocyanate (FITC)-conjugated anti-CD11b antibody (M1/70), FITC-conjugated anti-I-Ab antibody (25-9-17), FITC-conjugated isotype-matched IgG controls, biotinylated anti-B7-1 antibody (16-10A1), biotinylated anti-B7-2 antibody (GL1), biotinylated anti-CD40 antibody (3/23), biotinylated anti-CD40 ligand antibody (MR1), biotinylated anti-I-Ab antibody (25-9-17), biotinylated isotype-matched IgG controls, phycoerythrin-conjugated streptavidin, and the Riboquant multiprobe RPA kit (mCK-1 and mCK-5) were obtained from PharMingen (San Diego, CA).

    Techniques: Recombinase Polymerase Amplification, Expressing

    Representative panels of RPA from transplanted hearts. Fifteen micrograms of total RNA from normal non-transplanted hearts, or from hearts explanted at 0 hour, 4 hour, 24 hour, 3 days, or 7 days after transplantation were analyzed using the multiprobe RPA kits mCK-3b (containing DNA templates for TNF-β, LT-β, TNF-α, IL-6, IFN-γ, IFN-β, TGF-β1, TGF-β2, TGF-β3, MIF, L32, and GAPDH) and mCK-2b (containing DNA templates for IL-12p35, IL-12p40, IL-10, IL-1α, IL-1β, IL-1Ra, IL-18, IL-6, IFN-γ, MIF, L32, and GAPDH). The lanes on the far left show the undigested labeled probes that serve as size references, and the lanes on the far right show the results of RPA using a control RNA sample that was supplied by a manufacturer of the kit. Left panel: Representative gel images of B/6 isografts experiment analyzed by mCK-3b. Right panel: Representative gel images of BALB/c to B/6 allografts experiment analyzed by mCK-3b.

    Journal: The American Journal of Pathology

    Article Title: Cold Ischemia Induces Isograft Arteriopathy, but Does Not Augment Allograft Arteriopathy in Non-Immunosuppressed Hosts

    doi:

    Figure Lengend Snippet: Representative panels of RPA from transplanted hearts. Fifteen micrograms of total RNA from normal non-transplanted hearts, or from hearts explanted at 0 hour, 4 hour, 24 hour, 3 days, or 7 days after transplantation were analyzed using the multiprobe RPA kits mCK-3b (containing DNA templates for TNF-β, LT-β, TNF-α, IL-6, IFN-γ, IFN-β, TGF-β1, TGF-β2, TGF-β3, MIF, L32, and GAPDH) and mCK-2b (containing DNA templates for IL-12p35, IL-12p40, IL-10, IL-1α, IL-1β, IL-1Ra, IL-18, IL-6, IFN-γ, MIF, L32, and GAPDH). The lanes on the far left show the undigested labeled probes that serve as size references, and the lanes on the far right show the results of RPA using a control RNA sample that was supplied by a manufacturer of the kit. Left panel: Representative gel images of B/6 isografts experiment analyzed by mCK-3b. Right panel: Representative gel images of BALB/c to B/6 allografts experiment analyzed by mCK-3b.

    Article Snippet: Multiprobe RNase protection assay kit mCK-3b (containing DNA templates for tumor necrosis factor (TNF)-β, lymphotoxin (LT)-β, TNF-α, interleukin (IL)−6, interferon (IFN)-γ, IFN-β, transforming growth factor (TGF)-β1, TGF-β2, TGF-β3, macrophage migration-inhibitory factor (MIF), L32 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) and mCK-2b (containing DNA templates for IL-12p35, IL-12p40, IL-10, IL-1α, IL-1β, IL-1 receptor antagonist (Ra), IL-18, IL-6, IFN-γ, MIF, L32, and GAPDH) were obtained from PharMingen.

    Techniques: Recombinase Polymerase Amplification, Transplantation Assay, Labeling

    Cytokine profile of splenocytes from Friend virus-infected mice after ODN treatment. (a) Levels of cytokine transcripts from splenocytes derived from Friend virus-infected CpG-treated mice (gray bars) and control ODN-inoculated mice (black bars) were compared by RNA protection assays. Cells were taken at 4 weeks postinfection and stimulated with phorbol-12-myristate13-acetate/ionomycin for 5 h. Band densities are expressed as percentages of specific cytokine band density of that of an internal housekeeping transcript band (GAPDH). Since CpG-ODN promote Th1 responses, only the typical Th1/Th2-type cytokines IL-2, IL-4, and IFN-γ are shown. The differences in mRNA levels between the groups of treated and untreated mice were statistically significant by Mann-Whitney test for IL-2 ( P

    Journal: Journal of Virology

    Article Title: Effective Postexposure Treatment of Retrovirus-Induced Disease with Immunostimulatory DNA Containing CpG Motifs

    doi: 10.1128/JVI.76.22.11397-11404.2002

    Figure Lengend Snippet: Cytokine profile of splenocytes from Friend virus-infected mice after ODN treatment. (a) Levels of cytokine transcripts from splenocytes derived from Friend virus-infected CpG-treated mice (gray bars) and control ODN-inoculated mice (black bars) were compared by RNA protection assays. Cells were taken at 4 weeks postinfection and stimulated with phorbol-12-myristate13-acetate/ionomycin for 5 h. Band densities are expressed as percentages of specific cytokine band density of that of an internal housekeeping transcript band (GAPDH). Since CpG-ODN promote Th1 responses, only the typical Th1/Th2-type cytokines IL-2, IL-4, and IFN-γ are shown. The differences in mRNA levels between the groups of treated and untreated mice were statistically significant by Mann-Whitney test for IL-2 ( P

    Article Snippet: Spleen cells from Friend virus-infected mice were depleted of red blood cells, and 107 cells were stimulated with 2 ng of phorbol-12-myristate13-acetate and 500 ng of ionomycin per ml for 5 h. Total RNA was isolated with Trizol (AppliChem, Darmstadt, Germany), and a commercial RNA protection assay (cytokine template set mCK-1; Pharmingen, Heidelberg, Germany) was performed according to the company's standard protocol.

    Techniques: Infection, Mouse Assay, Derivative Assay, MANN-WHITNEY