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plenti x1 hygro mcherry ramp4  (Addgene inc)


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    Structured Review

    Addgene inc plenti x1 hygro mcherry ramp4
    Plenti X1 Hygro Mcherry Ramp4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry/pLenti-X1-hygro-mCherry-RAMP4+(Plasmid+%23118391)/pm42848836-254-0-7
    Average 92 stars, based on 7 article reviews
    plenti x1 hygro mcherry ramp4 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Virus:

    Article Title: Cocaine induced astrocytic activation was associated with its vasoconstricting effects independent of its neuronal effects in the prefrontal cortex.
    Article Snippet: .. Specifically, a mixture of two viruses (0.5μl /each virus) AAV5.CAG.Flex.GCaMP6f.WPRE.SV40 (AV-5-PV2816, Penn Vector Core) with AAV5.GFAP. hM3D(Gq). mCherry (50479-AAV5, Addgene) were slowly infused at 0.1 μl/min into transgenic mice (GFAP-Cre, Figure 1ci) to express GCaMP6f in astrocytes in the right PFC [A/P: +2; M/L: -0.75; D/V: -0.5 mm]. .. To express GCaMP6f into neurons, 0.5μl AAV5.Syn.GCaMP6f.WPRE.SV40 (AV-5-PV2822, Penn Vector Core) mix with 0.5ul AAV5.GFAP. hM3D(Gq). mCherry, were infused into the same brain region of wild type mice (C57BL/6, Figure 1cii).

    Transgenic Assay:

    Article Title: Cocaine induced astrocytic activation was associated with its vasoconstricting effects independent of its neuronal effects in the prefrontal cortex.
    Article Snippet: .. Specifically, a mixture of two viruses (0.5μl /each virus) AAV5.CAG.Flex.GCaMP6f.WPRE.SV40 (AV-5-PV2816, Penn Vector Core) with AAV5.GFAP. hM3D(Gq). mCherry (50479-AAV5, Addgene) were slowly infused at 0.1 μl/min into transgenic mice (GFAP-Cre, Figure 1ci) to express GCaMP6f in astrocytes in the right PFC [A/P: +2; M/L: -0.75; D/V: -0.5 mm]. .. To express GCaMP6f into neurons, 0.5μl AAV5.Syn.GCaMP6f.WPRE.SV40 (AV-5-PV2822, Penn Vector Core) mix with 0.5ul AAV5.GFAP. hM3D(Gq). mCherry, were infused into the same brain region of wild type mice (C57BL/6, Figure 1cii).

    Plasmid Preparation:

    Article Title: A live-cell autophagy reporter reveals reversible vacuolation in naked mole-rat skin fibroblasts under lysosomal stress
    Article Snippet: .. The pDEST-CMV mCherry–EGFP–LC3 WT human plasmid expressing an mCherry–EGFP–LC3 fusion protein was a gift from Robin Kettler (Addgene plasmid #123230) [ ]. ..

    Article Title: Using DIPA-CRISPR for simple and efficient endogenous protein tagging in insects
    Article Snippet: .. We thank the Multicellgenome laboratory (IBE, CSIC-UPF) for kindly providing the plasmid containing mCherry (Addgene plasmid no. 111874). ..

    Article Title: A 3D multi-compartment assembloid to study combined immune cell infiltration and cytotoxicity
    Article Snippet: Data analysis was performed using FlowJo software. .. To generate the OVCAR3 and JAR luciferase-expressing mCherry-tagged cell lines, the luciferase (Addgene 46793) and mCherry (Addgene 84020) sequences were inserted into the pSLCAR-CD19-BBz (Addgene 135992) plasmid via Gibson assembly (NEB E5520S). ..

    Article Title: Projections from thalamic nucleus reuniens to medial septum enable extinction of remote fear memory.
    Article Snippet: Mice were anesthetized with isoflurane (5% for in‐ duction, 1.5‐2.0% after) and fixed in the stereotactic frame (51503, Stoelting, Wood Dale, IL, USA). .. AAV2.1 [camk2a_mCherry (titer 7.5 × 107 pc/μl, from Karl De‐ isseroth’s Lab, Addgene plasmid #114469); hSyn_hM‐ 4D(Gi)_mCherry (4.59 × 109 pc/ul; Addgene plasmid #50475)] were injected into RE (250 nl, coordinates from the Bregma: AP, ‐0.58 mm; ML, ±0.1 mm; DV, 32 Acta Neurobiol Exp 2026, 86: 30–52 Thalamic circuit for remote memory extinctionActa Neurobiol Exp 2026, 86 ‐4.3 mm), MS (150 nl, coordinates from the Bregma: AP, ‐0.75 mm; ML, ± 0.0 mm; DV, ‐4.1 mm), AAV9: hSyn_ DiO_hM4Di‐mCherry (3.3 × 109 pc/μl; constructed by the VVF [dlox‐hM4D(Gi)_mCherry(rev)‐dlox: Addgene #50461]) and AAV9: hSyn_DIO_mCherry (3.4 × 109 pc/μl; constructed by the VVF) and AAV9: camk2a_hM4D(Gi)_ mCherry (4.4 × 109 pc/ul; Addgene plasmid #50477) were injected into RE; rAAV1: iCre_EGFP (3.6 × 109 pc/μl) con‐ structed by the VVF [iCre: Addgene #24593] and rAAV1: hSyn‐EGFP (1.7 × 1011 pc/μl) was injected into the MS (Paxinos and Franklin, 2019). ..

    Expressing:

    Article Title: A live-cell autophagy reporter reveals reversible vacuolation in naked mole-rat skin fibroblasts under lysosomal stress
    Article Snippet: .. The pDEST-CMV mCherry–EGFP–LC3 WT human plasmid expressing an mCherry–EGFP–LC3 fusion protein was a gift from Robin Kettler (Addgene plasmid #123230) [ ]. ..

    other:

    Article Title: A live-cell autophagy reporter reveals reversible vacuolation in naked mole-rat skin fibroblasts under lysosomal stress
    Article Snippet: A killing curve for HeLa Kyoto cells with a range of G418 Sulfate (Gibco) concentrations was made to determine 600 μg/ml as the selective concentration.

    Injection:

    Article Title: Acetylcholine demixes heterogeneous dopamine signals for learning and moving.
    Article Snippet: For fiber photometry experiment with separate dopamine (n = 6 rats) and acetylcholine (n = 6 rats) recordings, rats were injected with 300–480 nl of either GRABDA2h (AAV9-hSyn-GRABDA2h; Addgene, 140554) or GRABACh (AAV9-hSyn-ACh4.3, WZ Biosciences, YL10002) into the DMS (AP = −0.4 mm, mediolateral (ML) = ±3.5 mm, dorsoventral (DV) = −4.6 mm or AP = 1.7 mm, ML = ±2.8 mm, DV = −4.3 mm from the skull surface at bregma at a 10∘ angle). .. To allow for brain motion correction, mCherry (AAV9-CB7-CI-mCherry-WPRE-RBG; Addgene, 105544) was injected as a control fluorophore by mixing with the GRAB sensors at a ratio of 1.5:1–2.5:1 before injection. ..

    Article Title: Projections from thalamic nucleus reuniens to medial septum enable extinction of remote fear memory.
    Article Snippet: Mice were anesthetized with isoflurane (5% for in‐ duction, 1.5‐2.0% after) and fixed in the stereotactic frame (51503, Stoelting, Wood Dale, IL, USA). .. AAV2.1 [camk2a_mCherry (titer 7.5 × 107 pc/μl, from Karl De‐ isseroth’s Lab, Addgene plasmid #114469); hSyn_hM‐ 4D(Gi)_mCherry (4.59 × 109 pc/ul; Addgene plasmid #50475)] were injected into RE (250 nl, coordinates from the Bregma: AP, ‐0.58 mm; ML, ±0.1 mm; DV, 32 Acta Neurobiol Exp 2026, 86: 30–52 Thalamic circuit for remote memory extinctionActa Neurobiol Exp 2026, 86 ‐4.3 mm), MS (150 nl, coordinates from the Bregma: AP, ‐0.75 mm; ML, ± 0.0 mm; DV, ‐4.1 mm), AAV9: hSyn_ DiO_hM4Di‐mCherry (3.3 × 109 pc/μl; constructed by the VVF [dlox‐hM4D(Gi)_mCherry(rev)‐dlox: Addgene #50461]) and AAV9: hSyn_DIO_mCherry (3.4 × 109 pc/μl; constructed by the VVF) and AAV9: camk2a_hM4D(Gi)_ mCherry (4.4 × 109 pc/ul; Addgene plasmid #50477) were injected into RE; rAAV1: iCre_EGFP (3.6 × 109 pc/μl) con‐ structed by the VVF [iCre: Addgene #24593] and rAAV1: hSyn‐EGFP (1.7 × 1011 pc/μl) was injected into the MS (Paxinos and Franklin, 2019). ..

    Control:

    Article Title: Acetylcholine demixes heterogeneous dopamine signals for learning and moving.
    Article Snippet: For fiber photometry experiment with separate dopamine (n = 6 rats) and acetylcholine (n = 6 rats) recordings, rats were injected with 300–480 nl of either GRABDA2h (AAV9-hSyn-GRABDA2h; Addgene, 140554) or GRABACh (AAV9-hSyn-ACh4.3, WZ Biosciences, YL10002) into the DMS (AP = −0.4 mm, mediolateral (ML) = ±3.5 mm, dorsoventral (DV) = −4.6 mm or AP = 1.7 mm, ML = ±2.8 mm, DV = −4.3 mm from the skull surface at bregma at a 10∘ angle). .. To allow for brain motion correction, mCherry (AAV9-CB7-CI-mCherry-WPRE-RBG; Addgene, 105544) was injected as a control fluorophore by mixing with the GRAB sensors at a ratio of 1.5:1–2.5:1 before injection. ..

    Luciferase:

    Article Title: A 3D multi-compartment assembloid to study combined immune cell infiltration and cytotoxicity
    Article Snippet: Data analysis was performed using FlowJo software. .. To generate the OVCAR3 and JAR luciferase-expressing mCherry-tagged cell lines, the luciferase (Addgene 46793) and mCherry (Addgene 84020) sequences were inserted into the pSLCAR-CD19-BBz (Addgene 135992) plasmid via Gibson assembly (NEB E5520S). ..

    Construct:

    Article Title: Projections from thalamic nucleus reuniens to medial septum enable extinction of remote fear memory.
    Article Snippet: Mice were anesthetized with isoflurane (5% for in‐ duction, 1.5‐2.0% after) and fixed in the stereotactic frame (51503, Stoelting, Wood Dale, IL, USA). .. AAV2.1 [camk2a_mCherry (titer 7.5 × 107 pc/μl, from Karl De‐ isseroth’s Lab, Addgene plasmid #114469); hSyn_hM‐ 4D(Gi)_mCherry (4.59 × 109 pc/ul; Addgene plasmid #50475)] were injected into RE (250 nl, coordinates from the Bregma: AP, ‐0.58 mm; ML, ±0.1 mm; DV, 32 Acta Neurobiol Exp 2026, 86: 30–52 Thalamic circuit for remote memory extinctionActa Neurobiol Exp 2026, 86 ‐4.3 mm), MS (150 nl, coordinates from the Bregma: AP, ‐0.75 mm; ML, ± 0.0 mm; DV, ‐4.1 mm), AAV9: hSyn_ DiO_hM4Di‐mCherry (3.3 × 109 pc/μl; constructed by the VVF [dlox‐hM4D(Gi)_mCherry(rev)‐dlox: Addgene #50461]) and AAV9: hSyn_DIO_mCherry (3.4 × 109 pc/μl; constructed by the VVF) and AAV9: camk2a_hM4D(Gi)_ mCherry (4.4 × 109 pc/ul; Addgene plasmid #50477) were injected into RE; rAAV1: iCre_EGFP (3.6 × 109 pc/μl) con‐ structed by the VVF [iCre: Addgene #24593] and rAAV1: hSyn‐EGFP (1.7 × 1011 pc/μl) was injected into the MS (Paxinos and Franklin, 2019). ..



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