Journal: Molecules

Article Title: The Effects of Different Thiol-Containing Compounds on the Degradation of Sulforaphene

doi: 10.3390/molecules29184328

Figure Lengend Snippet: The concentration of each substance in radish seeds hydrolytic process. ( A – C ) the concentrations of SFE, HS − -SFE product, and Cys-SFE product 2 detected by HPLC analysis with PDA detector; ( D – F ) HS − , Cys, and GSH assayed by HPLC after mBBr derivatization with fluorescence detector. The radish seeds’ powders and PBS buffer were added to a screw-plugged tube at a ratio of 1:10, and the mixture was reacted at 25 °C. At different time intervals, 1 mL of the mixture were taken, centrifuged, and filtered with 0.22 μm filter. Part of the filtrate was used to detect HS − , Cys, and GSH after mBBr derivatization, and others was injected to HPLC to analyze the contents of SFE and its partial degradation products.

Article Snippet: Monobromodimane (mBBr) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Concentration Assay, Fluorescence, Injection

Journal: Molecules

Article Title: The Effects of Different Thiol-Containing Compounds on the Degradation of Sulforaphene

doi: 10.3390/molecules29184328

Figure Lengend Snippet: The concentration of each substance in radish seed hydrolytic process with or without sulfide-oxidizing bacteria. ( A – C ) the concentrations of SFE, HS − -SFE product, and Cys-SFE product 2 detected by HPLC analysis with PDA detector; ( D – F ) HS − , Cys, and GSH assayed by HPLC after mBBr derivatization with fluorescence detector. Ten mL OD 600 = 5 Cp wt or Cp ko resuspension was added into 1 g seed powders, respectively. The reaction was carried out at 25 °C for 6 h. At different time intervals, 1 mL mixture were taken, centrifuged, and filtered with 0.22 μm filter. Part of the filtrate was used to detect HS − , Cys, and GSH after mBBr derivatization, and others were injected to HPLC to analyze the contents of SFE and its partial gradation products.

Article Snippet: Monobromodimane (mBBr) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Concentration Assay, Bacteria, Fluorescence, Injection

Journal: Molecules

Article Title: The Effects of Different Thiol-Containing Compounds on the Degradation of Sulforaphene

doi: 10.3390/molecules29184328

Figure Lengend Snippet: The concentration of each substance in the hydrolytic process of heat-treatment seeds. ( A – C ) the concentrations of SFE, HS − -SFE product, and Cys-SFE product 2 detected by HPLC analysis with PDA detector; ( D – F ) HS − , Cys, and GSH assayed by HPLC after mBBr derivatization with fluorescence detector. The seed powders and PBS buffer were added to a screw-plugged tube at a ratio of 1:10, and the mixture reacted at 25 °C. At different time intervals, 1 mL of the mixture was taken, centrifuged, and filtered with 0.22 μm filter. Part of the filtrate was used to detect HS − , Cys, and GSH after mBBr derivatization, and others were injected to HPLC to analyze the contents of SFE and its partial degradation products.

Article Snippet: Monobromodimane (mBBr) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Concentration Assay, Fluorescence, Injection