mbb  (Millipore)


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  • 99
    Name:
    TWEEN 20
    Description:

    Catalog Number:
    p5927
    Price:
    None
    Applications:
    Non-ionic detergent
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    Structured Review

    Millipore mbb
    TWEEN 20

    https://www.bioz.com/result/mbb/product/Millipore
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    mbb - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Concentration Assay:

    Article Title: Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing
    Article Snippet: .. Crude chemical/enzymatic lysis method (C) Tween 20 (Sigma-Aldrich, St. Louis, MO) at a final concentration of 0.5% v/v and Proteinase K (Promega, Madison, WI) at a final concentration of 0.2 mg mL−1 were added to samples, which were then incubated at 55°C for 2 h and then at 95°C for 5 min. One microliter of this lysate was directly used in PCR reactions. ..

    Article Title: Dynamin-2 Stabilizes the HIV-1 Fusion Pore with a Low Oligomeric State
    Article Snippet: .. The concentration of T20 (Sigma) used to inhibit cell-cell fusion was 40 μg/mL. .. Time-resolved single virus tracking with TIRFM was performed on TZM-bl cells expressing DNM2-mCherry (Addgene) that were grown to near confluency on glass-bottom 35 mm Petri dishes (MatTek) in phenol red-free growth medium.

    Incubation:

    Article Title: Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing
    Article Snippet: .. Crude chemical/enzymatic lysis method (C) Tween 20 (Sigma-Aldrich, St. Louis, MO) at a final concentration of 0.5% v/v and Proteinase K (Promega, Madison, WI) at a final concentration of 0.2 mg mL−1 were added to samples, which were then incubated at 55°C for 2 h and then at 95°C for 5 min. One microliter of this lysate was directly used in PCR reactions. ..

    Article Title: Comparison of the Effects of Pathogenic Simian Human Immunodeficiency Virus Strains SHIV-89.6P and SHIV-KU2 in Cynomolgus Macaques
    Article Snippet: .. Plates were incubated with antigen for 40 h at 37°C/5% CO2 , and then washed for 10 min with distilled water followed by four washes with ELISPOT wash buffer [D-PBS containing 0.05% Tween-20 (Sigma)]. .. The plates were then incubated with 2.5 μ g/ml biotinylated antihuman IFN- γ antibody (mAb7-B61-Biotin, Mabtech) for 2 h at 37°C/5% CO2 and washed four times with ELISPOT wash buffer.

    Enzyme-linked Immunospot:

    Article Title: Comparison of the Effects of Pathogenic Simian Human Immunodeficiency Virus Strains SHIV-89.6P and SHIV-KU2 in Cynomolgus Macaques
    Article Snippet: .. Plates were incubated with antigen for 40 h at 37°C/5% CO2 , and then washed for 10 min with distilled water followed by four washes with ELISPOT wash buffer [D-PBS containing 0.05% Tween-20 (Sigma)]. .. The plates were then incubated with 2.5 μ g/ml biotinylated antihuman IFN- γ antibody (mAb7-B61-Biotin, Mabtech) for 2 h at 37°C/5% CO2 and washed four times with ELISPOT wash buffer.

    Polymerase Chain Reaction:

    Article Title: Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing
    Article Snippet: .. Crude chemical/enzymatic lysis method (C) Tween 20 (Sigma-Aldrich, St. Louis, MO) at a final concentration of 0.5% v/v and Proteinase K (Promega, Madison, WI) at a final concentration of 0.2 mg mL−1 were added to samples, which were then incubated at 55°C for 2 h and then at 95°C for 5 min. One microliter of this lysate was directly used in PCR reactions. ..

    Lysis:

    Article Title: Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing
    Article Snippet: .. Crude chemical/enzymatic lysis method (C) Tween 20 (Sigma-Aldrich, St. Louis, MO) at a final concentration of 0.5% v/v and Proteinase K (Promega, Madison, WI) at a final concentration of 0.2 mg mL−1 were added to samples, which were then incubated at 55°C for 2 h and then at 95°C for 5 min. One microliter of this lysate was directly used in PCR reactions. ..

    other:

    Article Title: Facile Histamine Detection by Surface-Enhanced Raman Scattering Using SiO2@Au@Ag Alloy Nanoparticles
    Article Snippet: Tetraethylorthosilicate (TEOS), 3-aminopropyltriethoxysilane (APTS), polyvinylpyrrolidone (PVP) (Mw 40,000), silver nitrate (AgNO3 ), L-ascorbic acid, Tween 20, tetrakis(hydroxymethyl)phosphonium chloride (THPC), gold (III) chloride trihydrate (HAuCl4 ), and histamine dihydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA).

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  • 96
    Millipore monobromobimane mbb
    Monobromobimane Mbb, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monobromobimane mbb/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monobromobimane mbb - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    85
    Millipore mbb buffer
    <t>MBB</t> reduces non-specific bindings in the <t>ELISA</t> assay. Serum samples NHS, M16, and M38 were diluted in either 1% BSA (left) or MBB buffer (right) for ELISA assay. For Tyrosinase (Tyr), antibody T9230 (US Biologicals) and biotinylated T311 were used as the capture antibody and the detection antibody, respectively. For IL-8, the IL-8 Cytoset from Biosource was used. To show non-specific bindings, Tyr or IL-8 capture antibodies were used but biotinylated anti-MIA or anti-VEGF antibodies were used respectively as the detection antibodies.
    Mbb Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mbb buffer/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mbb buffer - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    91
    Millipore mbb
    Blocking TLR4 inhibited intracellular oxidative stress and prevented SR Ca 2+ leak in LPS-stimulated cardiomyocytes. A , representative images of MitoSOX red fluorescence recorded from control, LPS, LPS + TAK-242, and TAK-242 groups. B , averages of MitoSOX fluorescence in four groups ( n = 38–49 cells in each group). C , representative images of <t>mBB</t> fluorescence intensity and Coomassie-stained gels in parallel in four groups. D , relative free thiol content (%) of <t>RyR2</t> measured by normalizing mBB fluorescence to RyR2 level ( n = 3 in each group). E , representative images and statistics of the frequency of Ca 2+ sparks ( n = 52–67 cells in each group). F , representative images and statistics of caffeine-elicited Ca 2+ transient ( n = 13–20 cells in each group). G , representative images and statistics of the amplitude of Ca 2+ transient and the maximum of cell shortening ( n = 37–50 in each group). *, p
    Mbb, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mbb/product/Millipore
    Average 91 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    mbb - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    MBB reduces non-specific bindings in the ELISA assay. Serum samples NHS, M16, and M38 were diluted in either 1% BSA (left) or MBB buffer (right) for ELISA assay. For Tyrosinase (Tyr), antibody T9230 (US Biologicals) and biotinylated T311 were used as the capture antibody and the detection antibody, respectively. For IL-8, the IL-8 Cytoset from Biosource was used. To show non-specific bindings, Tyr or IL-8 capture antibodies were used but biotinylated anti-MIA or anti-VEGF antibodies were used respectively as the detection antibodies.

    Journal: International Journal of Molecular Sciences

    Article Title: IL8 and Cathepsin B as Melanoma Serum Biomarkers

    doi: 10.3390/ijms12031505

    Figure Lengend Snippet: MBB reduces non-specific bindings in the ELISA assay. Serum samples NHS, M16, and M38 were diluted in either 1% BSA (left) or MBB buffer (right) for ELISA assay. For Tyrosinase (Tyr), antibody T9230 (US Biologicals) and biotinylated T311 were used as the capture antibody and the detection antibody, respectively. For IL-8, the IL-8 Cytoset from Biosource was used. To show non-specific bindings, Tyr or IL-8 capture antibodies were used but biotinylated anti-MIA or anti-VEGF antibodies were used respectively as the detection antibodies.

    Article Snippet: To study if additional serum proteins can also be used to characterize melanoma, we also performed multiplex ELISA assays in MBB buffer using the Milliplex cytokine/chemokine kit for 39 human target proteins (Millipore) for sera from 11 melanoma patients.

    Techniques: Enzyme-linked Immunosorbent Assay

    Blocking TLR4 inhibited intracellular oxidative stress and prevented SR Ca 2+ leak in LPS-stimulated cardiomyocytes. A , representative images of MitoSOX red fluorescence recorded from control, LPS, LPS + TAK-242, and TAK-242 groups. B , averages of MitoSOX fluorescence in four groups ( n = 38–49 cells in each group). C , representative images of mBB fluorescence intensity and Coomassie-stained gels in parallel in four groups. D , relative free thiol content (%) of RyR2 measured by normalizing mBB fluorescence to RyR2 level ( n = 3 in each group). E , representative images and statistics of the frequency of Ca 2+ sparks ( n = 52–67 cells in each group). F , representative images and statistics of caffeine-elicited Ca 2+ transient ( n = 13–20 cells in each group). G , representative images and statistics of the amplitude of Ca 2+ transient and the maximum of cell shortening ( n = 37–50 in each group). *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Toll-like receptor 4–induced ryanodine receptor 2 oxidation and sarcoplasmic reticulum Ca2+ leakage promote cardiac contractile dysfunction in sepsis

    doi: 10.1074/jbc.M117.812289

    Figure Lengend Snippet: Blocking TLR4 inhibited intracellular oxidative stress and prevented SR Ca 2+ leak in LPS-stimulated cardiomyocytes. A , representative images of MitoSOX red fluorescence recorded from control, LPS, LPS + TAK-242, and TAK-242 groups. B , averages of MitoSOX fluorescence in four groups ( n = 38–49 cells in each group). C , representative images of mBB fluorescence intensity and Coomassie-stained gels in parallel in four groups. D , relative free thiol content (%) of RyR2 measured by normalizing mBB fluorescence to RyR2 level ( n = 3 in each group). E , representative images and statistics of the frequency of Ca 2+ sparks ( n = 52–67 cells in each group). F , representative images and statistics of caffeine-elicited Ca 2+ transient ( n = 13–20 cells in each group). G , representative images and statistics of the amplitude of Ca 2+ transient and the maximum of cell shortening ( n = 37–50 in each group). *, p

    Article Snippet: The content of the free thiols ( i.e. the number of reduced cysteines) in RyR2 in cardiomyocytes was determined with the mBB (Calbiochem) fluorescence technique ( , ).

    Techniques: Blocking Assay, Fluorescence, Staining