maxi k beta antibody  (Alomone Labs)


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    Alomone Labs maxi k beta antibody
    Maxi K Beta Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maxi k beta antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    maxi k beta antibody - by Bioz Stars, 2022-08
    93/100 stars

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    Alomone Labs kcnmb4
    BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of <t>KCNMB4</t> in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.
    Kcnmb4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kcnmb4/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
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    kcnmb4 - by Bioz Stars, 2022-08
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    94
    Alomone Labs bk β1 subunit
    Mitochondrial calcium uptake is impaired by Paxilline and by genetic ablation of <t>β1-subunit</t> A) Paxilline reduces the amount of mitochondrial Ca 2+ necessary to induce formation and opening of mPTP. B) Mitochondria from the β1 KO required less Ca 2+ than mitochondria from the Wt to trigger the opening of mPTP. C) Calcium retention capacity values obtained in A for paxilline and in B for the β1-KO mitochondria. Bars SEM, (* p
    Bk β1 Subunit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bk β1 subunit/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of KCNMB4 in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.

    Journal: British Journal of Pharmacology

    Article Title: Big conductance calcium‐activated potassium channel openers control spasticity without sedation) Big conductance calcium‐activated potassium channel openers control spasticity without sedation

    doi: 10.1111/bph.13889

    Figure Lengend Snippet: BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of KCNMB4 in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.

    Article Snippet: Membranes were washed, blocked for 1 h in blocking buffer (5% non‐fat dried milk) and incubated in blocking buffer with primary antibody using rabbit polyclonal antibodies against mouse/human KCNMA1 (APC‐021, previously validated by lack of activity in big conductance calcium‐activated potassium channel Kcnma1 ‐deficient mice and APC‐151 antibody) and KCNMB4 (APC‐061 antibody, previously validated by lack of activity in Kcnmb4‐deficient mice despite detecting multiple isoforms), which were purchased from Alomone Labs, Jerusalem Israel, whose website reports supporting literature concerning characterization of the antibodies.

    Techniques: Expressing, Western Blot, Mouse Assay, Injection

    Effects of BK-β4 small interfering (si) RNA on shear-stress (10 dynes/cm 2 )-induced K efflux from C11. A : C11 were untreated (normal), transfected with green fluorescent protein (control), 4 nontarget siRNA oligos (siRNA nontarget), or 6 siRNA

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Shear stress-induced volume decrease in C11-MDCK cells by BK-?/?4

    doi: 10.1152/ajprenal.00222.2010

    Figure Lengend Snippet: Effects of BK-β4 small interfering (si) RNA on shear-stress (10 dynes/cm 2 )-induced K efflux from C11. A : C11 were untreated (normal), transfected with green fluorescent protein (control), 4 nontarget siRNA oligos (siRNA nontarget), or 6 siRNA

    Article Snippet: Primary antibodies included anti-BK-α (rabbit polyclonal, diluted 1:1,000, Alomone Labs), anti-BK-β4 (rabbit polyclonal, diluted 1:1,000, Alomone Labs), and anti-actin (rabbit polyclonal, 1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA) with either donkey (Santa Cruz Biotechnology) or goat (Pierce Biotechnology) anti-rabbit IgG-conjugated horseradish peroxidase secondary antibody diluted 1:20,000–1:40,000.

    Techniques: Transfection

    Role of aldosterone in expression of BK-α and BK-β4.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Regulation of BK-α expression in the distal nephron by aldosterone and urine pH

    doi: 10.1152/ajprenal.00171.2013

    Figure Lengend Snippet: Role of aldosterone in expression of BK-α and BK-β4.

    Article Snippet: Primary antibodies included anti-BK-α (mouse monoclonal, diluted 1:200; Neuromab), anti-BK-β4 (rabbit polyclonal, diluted 1:500; Alomone Laboratories), anti-cadherin (goat polyclonal, diluted 1:500; Santa Cruz Biotechnology), anti-GAPDH (goat polyclonal, diluted 1:500, Santa Cruz Biotechnology), and anti-β-actin (mouse monoclonal, diluted 1:500; Santa Cruz Biotechnology) with goat anti-rabbit IgG, donkey anti-mouse IgG, or donkey anti-goat IgG-conjugated horseradish peroxidase (diluted 1:10,000–1:20,000; Santa Cruz Biotechnology).

    Techniques: Expressing

    Western blot analysis of renal BK-α and BK- β4 expressions in mice with acid, neutral or alkaline drinking water. Representative immunoblots of total cellular BK-α expression ( A ) and summary bar plots of total BK-β4 expression

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Regulation of BK-α expression in the distal nephron by aldosterone and urine pH

    doi: 10.1152/ajprenal.00171.2013

    Figure Lengend Snippet: Western blot analysis of renal BK-α and BK- β4 expressions in mice with acid, neutral or alkaline drinking water. Representative immunoblots of total cellular BK-α expression ( A ) and summary bar plots of total BK-β4 expression

    Article Snippet: Primary antibodies included anti-BK-α (mouse monoclonal, diluted 1:200; Neuromab), anti-BK-β4 (rabbit polyclonal, diluted 1:500; Alomone Laboratories), anti-cadherin (goat polyclonal, diluted 1:500; Santa Cruz Biotechnology), anti-GAPDH (goat polyclonal, diluted 1:500, Santa Cruz Biotechnology), and anti-β-actin (mouse monoclonal, diluted 1:500; Santa Cruz Biotechnology) with goat anti-rabbit IgG, donkey anti-mouse IgG, or donkey anti-goat IgG-conjugated horseradish peroxidase (diluted 1:10,000–1:20,000; Santa Cruz Biotechnology).

    Techniques: Western Blot, Mouse Assay, Expressing

    Role of acid-base consumption on expression of BK-α and BK-β4.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Regulation of BK-α expression in the distal nephron by aldosterone and urine pH

    doi: 10.1152/ajprenal.00171.2013

    Figure Lengend Snippet: Role of acid-base consumption on expression of BK-α and BK-β4.

    Article Snippet: Primary antibodies included anti-BK-α (mouse monoclonal, diluted 1:200; Neuromab), anti-BK-β4 (rabbit polyclonal, diluted 1:500; Alomone Laboratories), anti-cadherin (goat polyclonal, diluted 1:500; Santa Cruz Biotechnology), anti-GAPDH (goat polyclonal, diluted 1:500, Santa Cruz Biotechnology), and anti-β-actin (mouse monoclonal, diluted 1:500; Santa Cruz Biotechnology) with goat anti-rabbit IgG, donkey anti-mouse IgG, or donkey anti-goat IgG-conjugated horseradish peroxidase (diluted 1:10,000–1:20,000; Santa Cruz Biotechnology).

    Techniques: Expressing

    Western blot analysis of renal BK-α and BK-β4 expression in WT on HK-alk diets treated with vehicle or spironolactone. Representative immunoblots ( A ) and summary bar plots of relative densitometries of total cell lysate BK-β4 (

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Regulation of BK-α expression in the distal nephron by aldosterone and urine pH

    doi: 10.1152/ajprenal.00171.2013

    Figure Lengend Snippet: Western blot analysis of renal BK-α and BK-β4 expression in WT on HK-alk diets treated with vehicle or spironolactone. Representative immunoblots ( A ) and summary bar plots of relative densitometries of total cell lysate BK-β4 (

    Article Snippet: Primary antibodies included anti-BK-α (mouse monoclonal, diluted 1:200; Neuromab), anti-BK-β4 (rabbit polyclonal, diluted 1:500; Alomone Laboratories), anti-cadherin (goat polyclonal, diluted 1:500; Santa Cruz Biotechnology), anti-GAPDH (goat polyclonal, diluted 1:500, Santa Cruz Biotechnology), and anti-β-actin (mouse monoclonal, diluted 1:500; Santa Cruz Biotechnology) with goat anti-rabbit IgG, donkey anti-mouse IgG, or donkey anti-goat IgG-conjugated horseradish peroxidase (diluted 1:10,000–1:20,000; Santa Cruz Biotechnology).

    Techniques: Western Blot, Expressing

    Western blot analysis of BK-β4 and PM BK-α expression in MDCK-C11 cells. Immunoblots ( A ) and summary bar plots ( B ) demonstrating the relative densitometries of cadherin/GAPDH in the PM, OM, and cytosolic (C) fractions of MDCK-C11 cells.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Regulation of BK-α expression in the distal nephron by aldosterone and urine pH

    doi: 10.1152/ajprenal.00171.2013

    Figure Lengend Snippet: Western blot analysis of BK-β4 and PM BK-α expression in MDCK-C11 cells. Immunoblots ( A ) and summary bar plots ( B ) demonstrating the relative densitometries of cadherin/GAPDH in the PM, OM, and cytosolic (C) fractions of MDCK-C11 cells.

    Article Snippet: Primary antibodies included anti-BK-α (mouse monoclonal, diluted 1:200; Neuromab), anti-BK-β4 (rabbit polyclonal, diluted 1:500; Alomone Laboratories), anti-cadherin (goat polyclonal, diluted 1:500; Santa Cruz Biotechnology), anti-GAPDH (goat polyclonal, diluted 1:500, Santa Cruz Biotechnology), and anti-β-actin (mouse monoclonal, diluted 1:500; Santa Cruz Biotechnology) with goat anti-rabbit IgG, donkey anti-mouse IgG, or donkey anti-goat IgG-conjugated horseradish peroxidase (diluted 1:10,000–1:20,000; Santa Cruz Biotechnology).

    Techniques: Western Blot, Expressing

    Effects of high-K diets and BK-β4 on BK-α expression.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Regulation of BK-α expression in the distal nephron by aldosterone and urine pH

    doi: 10.1152/ajprenal.00171.2013

    Figure Lengend Snippet: Effects of high-K diets and BK-β4 on BK-α expression.

    Article Snippet: Primary antibodies included anti-BK-α (mouse monoclonal, diluted 1:200; Neuromab), anti-BK-β4 (rabbit polyclonal, diluted 1:500; Alomone Laboratories), anti-cadherin (goat polyclonal, diluted 1:500; Santa Cruz Biotechnology), anti-GAPDH (goat polyclonal, diluted 1:500, Santa Cruz Biotechnology), and anti-β-actin (mouse monoclonal, diluted 1:500; Santa Cruz Biotechnology) with goat anti-rabbit IgG, donkey anti-mouse IgG, or donkey anti-goat IgG-conjugated horseradish peroxidase (diluted 1:10,000–1:20,000; Santa Cruz Biotechnology).

    Techniques: Expressing

    Mitochondrial calcium uptake is impaired by Paxilline and by genetic ablation of β1-subunit A) Paxilline reduces the amount of mitochondrial Ca 2+ necessary to induce formation and opening of mPTP. B) Mitochondria from the β1 KO required less Ca 2+ than mitochondria from the Wt to trigger the opening of mPTP. C) Calcium retention capacity values obtained in A for paxilline and in B for the β1-KO mitochondria. Bars SEM, (* p

    Journal: The Journal of physiology

    Article Title: MitoBKCa channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria

    doi: 10.1113/JP277769

    Figure Lengend Snippet: Mitochondrial calcium uptake is impaired by Paxilline and by genetic ablation of β1-subunit A) Paxilline reduces the amount of mitochondrial Ca 2+ necessary to induce formation and opening of mPTP. B) Mitochondria from the β1 KO required less Ca 2+ than mitochondria from the Wt to trigger the opening of mPTP. C) Calcium retention capacity values obtained in A for paxilline and in B for the β1-KO mitochondria. Bars SEM, (* p

    Article Snippet: Polyclonal antibody against BKCa (Rabbit Anti-KCNMA1 Cat. APC-021 and APC-151), and polyclonal antibody for BK-β1 subunit (Rabbit Anti-KCNMB1, Cat. APC-036) were from Alomone labs.

    Techniques:

    MitoBK Ca alpha and auxiliary β1 subunit association in cardiac mitochondria. A) Immunoprecipitation (IP) of BK Ca alpha and BK-β1 subunit with an antibody against BK Ca ). Taken together, these results indicates an association between mitoBK Ca and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBK Ca in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBK Ca recorded from Wt mitoplasts. The P o of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca 2+ . C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca 2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBK Ca channel activity with a P o of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca 2+ . D) Plots of I vs. V at 12 µM matrix Ca 2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of P o vs. V for mitoBK Ca channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.

    Journal: The Journal of physiology

    Article Title: MitoBKCa channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria

    doi: 10.1113/JP277769

    Figure Lengend Snippet: MitoBK Ca alpha and auxiliary β1 subunit association in cardiac mitochondria. A) Immunoprecipitation (IP) of BK Ca alpha and BK-β1 subunit with an antibody against BK Ca ). Taken together, these results indicates an association between mitoBK Ca and its auxiliary β1 subunit. Having demonstrated this, we characterized the biophysical properties of mitoBK Ca in Wt and β1-KO cardiac mitoplasts. B) Single-channel currents of mitoBK Ca recorded from Wt mitoplasts. The P o of the channel was 0.9 ± 0.08, n=4, in average at +80 mV and the indicated matrix Ca 2+ . C) Representative traces of a mitoplast from the β1 KO, were no single-channel current was detected at the indicated voltages and matrix Ca 2+ (upper traces). Few mitoplasts (5 out of 28, mitoplasts, n=5 hearts) showed mitoBK Ca channel activity with a P o of 0.4 ± 0.004, n=3 at +40 mV in the indicated matrix Ca 2+ . D) Plots of I vs. V at 12 µM matrix Ca 2+ from Wt and β1 KO mitoplasts (data from 6 and 3 patches, respectively). A slope conductance of 302±26 (n=4) and 335 ±15 pS (n=3) was calculated for Wt and β1 KO, respectively. E) Plots of P o vs. V for mitoBK Ca channels, data points represent the average of 4 and 3 mitoplasts for Wt and BK-β1 KO, respectively. Bars SEM.

    Article Snippet: Polyclonal antibody against BKCa (Rabbit Anti-KCNMA1 Cat. APC-021 and APC-151), and polyclonal antibody for BK-β1 subunit (Rabbit Anti-KCNMB1, Cat. APC-036) were from Alomone labs.

    Techniques: Immunoprecipitation, Activity Assay

    Localization of BKDEC and β1 subunit in HeLa cells. A) Cultured cells co-transfected with BKDEC alpha+β1subunit, loaded with mitotracker red, and DAPI B) β1-flag labeled with an anti-flag C) BKDEC labeled with anti-HA. D) Merge of mitotracker red, BKDEC and β1. E) Cross-correlation index (CCi), relative to mitotracker red signal, calculated from cells overexpressing BKDEC alone (Cci=0.53±0.05) and co-expressed with β1 subunit (Cci=0.77±0.01, n=11 cells from 3 different preparations).*p

    Journal: The Journal of physiology

    Article Title: MitoBKCa channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria

    doi: 10.1113/JP277769

    Figure Lengend Snippet: Localization of BKDEC and β1 subunit in HeLa cells. A) Cultured cells co-transfected with BKDEC alpha+β1subunit, loaded with mitotracker red, and DAPI B) β1-flag labeled with an anti-flag C) BKDEC labeled with anti-HA. D) Merge of mitotracker red, BKDEC and β1. E) Cross-correlation index (CCi), relative to mitotracker red signal, calculated from cells overexpressing BKDEC alone (Cci=0.53±0.05) and co-expressed with β1 subunit (Cci=0.77±0.01, n=11 cells from 3 different preparations).*p

    Article Snippet: Polyclonal antibody against BKCa (Rabbit Anti-KCNMA1 Cat. APC-021 and APC-151), and polyclonal antibody for BK-β1 subunit (Rabbit Anti-KCNMB1, Cat. APC-036) were from Alomone labs.

    Techniques: Cell Culture, Transfection, Labeling