mature bdnf  (Alomone Labs)


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    Structured Review

    Alomone Labs mature bdnf
    Line-blot for qualitative analysis of <t>anti-BDNF</t> antibodies specificity. ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R D <t>System-Quantikine</t> ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.
    Mature Bdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mature bdnf/product/Alomone Labs
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    mature bdnf - by Bioz Stars, 2022-10
    94/100 stars

    Images

    1) Product Images from "A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays"

    Article Title: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

    Journal: Scientific Reports

    doi: 10.1038/srep17989

    Line-blot for qualitative analysis of anti-BDNF antibodies specificity. ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R D System-Quantikine ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.
    Figure Legend Snippet: Line-blot for qualitative analysis of anti-BDNF antibodies specificity. ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R D System-Quantikine ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Negative Control, Dot Blot

    2) Product Images from "A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays"

    Article Title: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

    Journal: Scientific Reports

    doi: 10.1038/srep17989

    Line-blot for qualitative analysis of anti-BDNF antibodies specificity. ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R D System-Quantikine ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.
    Figure Legend Snippet: Line-blot for qualitative analysis of anti-BDNF antibodies specificity. ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R D System-Quantikine ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Negative Control, Dot Blot

    3) Product Images from "p75 neurotrophin receptor and pro-BDNF promote cell survival and migration in clear cell renal cell carcinoma"

    Article Title: p75 neurotrophin receptor and pro-BDNF promote cell survival and migration in clear cell renal cell carcinoma

    Journal: Oncotarget

    doi: 10.18632/oncotarget.8911

    Study of prosurvival signaling pathways and immunoprecipitated receptors complex AKT, ERK1/2 and p38MAPK signaling pathways were analyzed in 786-O cell line cultured in starving conditions. Response to pro-BDNF alone (20 ng/mL) A. or with GM6001 (20 μM) B. was evaluated at indicated time points. C. Immunoprecipitation was performed for TrkB or sortilin in presence or absence or FBS or pro-BDNF (20 ng/mL) as indicated in figure. 5% (v/v) of whole cell lysate (WCL) was used as a control by western blotting in this experiment. D. 786-O cell line was transfected with control (siRNA_C) or Sortilin siRNA (siRNA_SORT1). After 24 hours starving 786-O_siC and 786-O_siSortilin cells were treated for one hour with pro-BDNF (20 ng/mL) before AKT and ERK activation studies. Western blots are representative of three independent experiments.
    Figure Legend Snippet: Study of prosurvival signaling pathways and immunoprecipitated receptors complex AKT, ERK1/2 and p38MAPK signaling pathways were analyzed in 786-O cell line cultured in starving conditions. Response to pro-BDNF alone (20 ng/mL) A. or with GM6001 (20 μM) B. was evaluated at indicated time points. C. Immunoprecipitation was performed for TrkB or sortilin in presence or absence or FBS or pro-BDNF (20 ng/mL) as indicated in figure. 5% (v/v) of whole cell lysate (WCL) was used as a control by western blotting in this experiment. D. 786-O cell line was transfected with control (siRNA_C) or Sortilin siRNA (siRNA_SORT1). After 24 hours starving 786-O_siC and 786-O_siSortilin cells were treated for one hour with pro-BDNF (20 ng/mL) before AKT and ERK activation studies. Western blots are representative of three independent experiments.

    Techniques Used: Immunoprecipitation, Cell Culture, Western Blot, Transfection, Activation Assay

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    Alomone Labs mature bdnf
    Line-blot for qualitative analysis of <t>anti-BDNF</t> antibodies specificity. ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R D <t>System-Quantikine</t> ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.
    Mature Bdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mature bdnf/product/Alomone Labs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mature bdnf - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    95
    Alomone Labs mbdnf
    Characterization of <t>proBDNF</t> monoclonal antibody 2B11. ( A ) ELISA assay for the immunoreactivity of 2B11 against human proBDNF prodomain, and human, rat and mice proBDNF proteins, and human mature BDNF <t>(mBDNF).</t> 2B11 has strong immunoreactivity against proBDNF and prodomain, but not mBDNF; ( B ) Representative Western blot of human proBDNF and mBDNF detected by 2B11 (dilution 1:2000), note that 2B11 specifically recognizes proBDNF, but not mBDNF. ( C ) Representative images of neurosphere radiant migration treated by proBDNF, mBDNF, sheep polyclonal anti-proBDNF antibody, mouse monoclonal anti-proBDNF antibody 2B11 and co-treatment. ( D ) Statistical analysis of neurosphere migration radiance assay (***P
    Mbdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mbdnf/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mbdnf - by Bioz Stars, 2022-10
    95/100 stars
      Buy from Supplier

    Image Search Results


    Line-blot for qualitative analysis of anti-BDNF antibodies specificity. ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R D System-Quantikine ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.

    Journal: Scientific Reports

    Article Title: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

    doi: 10.1038/srep17989

    Figure Lengend Snippet: Line-blot for qualitative analysis of anti-BDNF antibodies specificity. ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R D System-Quantikine ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.

    Article Snippet: Of note, Aviscera-Bioscience and R & D System-Quantikine® kits were claimed to be specific for mature BDNF and to have some cross-reactivity with the human pro-BDNF.

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Dot Blot

    The mBDNF-TrkB receptor signaling is not impaired in the presence of aprotinin. ( A, B ) Mean native BDNF mRNA ( A , 4 intact hippocampi for each condition) and protein ( B , 4 slices for each condition) levels in control ACSF or aprotinin-treated tissue. (

    Journal: Cerebral Cortex (New York, NY)

    Article Title: NMDA-Dependent Switch of proBDNF Actions on Developing GABAergic Synapses

    doi: 10.1093/cercor/bhs071

    Figure Lengend Snippet: The mBDNF-TrkB receptor signaling is not impaired in the presence of aprotinin. ( A, B ) Mean native BDNF mRNA ( A , 4 intact hippocampi for each condition) and protein ( B , 4 slices for each condition) levels in control ACSF or aprotinin-treated tissue. (

    Article Snippet: 1,2,3,4-Tetrahydro-6-nitro-2,3-dioxo-benzo[f]quinoxaline-7-sulfonamide (NBQX), Gabazin, (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d] cyclohepten-5,10-imine maleate (MK801) and D-2-amino-5-phosphovaleric acid (D-AP5) (Tocris Cookson); k252a, TAT-pep5 (Calbiochem); Nifedipine, aprotinin (Sigma); p75NTR antibody (ab1554, Millipore); cleavage-resistant (CR) proBDNF, mBDNF (Alomone labs); TrkA-IgG, TrkB-IgG (R & D system).

    Techniques:

    Characterization of proBDNF monoclonal antibody 2B11. ( A ) ELISA assay for the immunoreactivity of 2B11 against human proBDNF prodomain, and human, rat and mice proBDNF proteins, and human mature BDNF (mBDNF). 2B11 has strong immunoreactivity against proBDNF and prodomain, but not mBDNF; ( B ) Representative Western blot of human proBDNF and mBDNF detected by 2B11 (dilution 1:2000), note that 2B11 specifically recognizes proBDNF, but not mBDNF. ( C ) Representative images of neurosphere radiant migration treated by proBDNF, mBDNF, sheep polyclonal anti-proBDNF antibody, mouse monoclonal anti-proBDNF antibody 2B11 and co-treatment. ( D ) Statistical analysis of neurosphere migration radiance assay (***P

    Journal: Scientific Reports

    Article Title: Peripheral Brain Derived Neurotrophic Factor Precursor Regulates Pain as an Inflammatory Mediator

    doi: 10.1038/srep27171

    Figure Lengend Snippet: Characterization of proBDNF monoclonal antibody 2B11. ( A ) ELISA assay for the immunoreactivity of 2B11 against human proBDNF prodomain, and human, rat and mice proBDNF proteins, and human mature BDNF (mBDNF). 2B11 has strong immunoreactivity against proBDNF and prodomain, but not mBDNF; ( B ) Representative Western blot of human proBDNF and mBDNF detected by 2B11 (dilution 1:2000), note that 2B11 specifically recognizes proBDNF, but not mBDNF. ( C ) Representative images of neurosphere radiant migration treated by proBDNF, mBDNF, sheep polyclonal anti-proBDNF antibody, mouse monoclonal anti-proBDNF antibody 2B11 and co-treatment. ( D ) Statistical analysis of neurosphere migration radiance assay (***P

    Article Snippet: 1, 3, 10, 30 ng proBDNF or mBDNF (Alomone Labs, Israel) also loaded onto a 10% SDS-PAGE gel.

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Western Blot, Migration