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Santa Cruz Biotechnology matrin 3 sirna
Matrin 3 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology lentivirus particles
Lentivirus Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology matrin 3 shrna
Matrin 3 Shrna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Structured Review

Santa Cruz Biotechnology matrin 3 sirna
Matrin 3 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matrin 3 sirna/product/Santa Cruz Biotechnology
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
matrin 3 sirna - by Bioz Stars, 2024-04
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Santa Cruz Biotechnology sirna sequences targeting matr3
Effect of LCH on the expression of <t>Matr3</t> proteins in the oral squamous cell carcinoma cell lines. Pull-down assays of LCH binding to Matr3 ex vivo . Whole (A) HCS2 and (B) HSC3 cell lysates were incubated with Sepharose 4B or LCH-Sepharose 4B beads overnight at 4°C. Immunoblotting of pull-down products was performed with the anti-Matr3 antibody. HSC2 and HSC3 cells were seeded onto a cell culture plate for 24 h and treated with LCH (10, 20 and 30 µM) for 48 h. (C) Cells were harvested and western blot analysis was performed via SDS-PAGE. Expression of Matr3, PARP, cleaved PARP, caspase-3, cleaved caspase-3 and GAPDH were detected by western blot analysis using specific antibodies. GAPDH protein was used as the loading control. LCH, licochalcone H; Matr3, matrin 3; PARP, poly (ADP-ribose) polymerase.
Sirna Sequences Targeting Matr3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Licochalcone H induces the apoptosis of human oral squamous cell carcinoma cells via regulation of matrin 3"

Article Title: Licochalcone H induces the apoptosis of human oral squamous cell carcinoma cells via regulation of matrin 3

Journal: Oncology Reports

doi: 10.3892/or.2018.6784

Effect of LCH on the expression of Matr3 proteins in the oral squamous cell carcinoma cell lines. Pull-down assays of LCH binding to Matr3 ex vivo . Whole (A) HCS2 and (B) HSC3 cell lysates were incubated with Sepharose 4B or LCH-Sepharose 4B beads overnight at 4°C. Immunoblotting of pull-down products was performed with the anti-Matr3 antibody. HSC2 and HSC3 cells were seeded onto a cell culture plate for 24 h and treated with LCH (10, 20 and 30 µM) for 48 h. (C) Cells were harvested and western blot analysis was performed via SDS-PAGE. Expression of Matr3, PARP, cleaved PARP, caspase-3, cleaved caspase-3 and GAPDH were detected by western blot analysis using specific antibodies. GAPDH protein was used as the loading control. LCH, licochalcone H; Matr3, matrin 3; PARP, poly (ADP-ribose) polymerase.
Figure Legend Snippet: Effect of LCH on the expression of Matr3 proteins in the oral squamous cell carcinoma cell lines. Pull-down assays of LCH binding to Matr3 ex vivo . Whole (A) HCS2 and (B) HSC3 cell lysates were incubated with Sepharose 4B or LCH-Sepharose 4B beads overnight at 4°C. Immunoblotting of pull-down products was performed with the anti-Matr3 antibody. HSC2 and HSC3 cells were seeded onto a cell culture plate for 24 h and treated with LCH (10, 20 and 30 µM) for 48 h. (C) Cells were harvested and western blot analysis was performed via SDS-PAGE. Expression of Matr3, PARP, cleaved PARP, caspase-3, cleaved caspase-3 and GAPDH were detected by western blot analysis using specific antibodies. GAPDH protein was used as the loading control. LCH, licochalcone H; Matr3, matrin 3; PARP, poly (ADP-ribose) polymerase.

Techniques Used: Expressing, Binding Assay, Ex Vivo, Incubation, Western Blot, Cell Culture, SDS Page

Knockdown of the expression of Matr3 results in induction of oral squamous cell carcinoma cell apoptosis. HSC2 and HSC3 cells were transfected with siCon or siMatr3. (A) mRNA expression level of Matr3 in each cell transfected with siCon or siMatr3 was analyzed by reverse transcription-polymerase chain reaction analysis. β-actin was used as a loading control. (B) siCon- or siMatr3-transfected HSC2 and HSC3 cell lysates were determined by western blot analysis using anti-Matr3, anti-caspase-3, anti-cleaved caspase-3, anti-PARP and anti-cleaved PARP antibodies. GAPDH was used as the loading control. (C) HSC2 and HSC3 cells were transfected with siCon or siMatr3 and soft agar assays were performed. Colony numbers and sizes were measured, and the results are expressed as the mean ± standard deviation (*P<0.05) for triplicate experiments. siMatr3, matrin 3-specific targeting siRNA; siCon, scrambled control siRNA; PARP, poly (ADP-ribose) polymerase.
Figure Legend Snippet: Knockdown of the expression of Matr3 results in induction of oral squamous cell carcinoma cell apoptosis. HSC2 and HSC3 cells were transfected with siCon or siMatr3. (A) mRNA expression level of Matr3 in each cell transfected with siCon or siMatr3 was analyzed by reverse transcription-polymerase chain reaction analysis. β-actin was used as a loading control. (B) siCon- or siMatr3-transfected HSC2 and HSC3 cell lysates were determined by western blot analysis using anti-Matr3, anti-caspase-3, anti-cleaved caspase-3, anti-PARP and anti-cleaved PARP antibodies. GAPDH was used as the loading control. (C) HSC2 and HSC3 cells were transfected with siCon or siMatr3 and soft agar assays were performed. Colony numbers and sizes were measured, and the results are expressed as the mean ± standard deviation (*P<0.05) for triplicate experiments. siMatr3, matrin 3-specific targeting siRNA; siCon, scrambled control siRNA; PARP, poly (ADP-ribose) polymerase.

Techniques Used: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Standard Deviation


Structured Review

Santa Cruz Biotechnology mouse matrin 3 sirna
Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the <t>Matrin-3</t> protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .
Mouse Matrin 3 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse matrin 3 sirna/product/Santa Cruz Biotechnology
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse matrin 3 sirna - by Bioz Stars, 2024-04
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1) Product Images from "Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells"

Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells

Journal: Scientific Reports

doi: 10.1038/s41598-018-31597-x

Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the Matrin-3 protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .
Figure Legend Snippet: Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the Matrin-3 protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .

Techniques Used: Mass Spectrometry, Expressing

In vitro and in vivo expression of Matrin-3 and phospho-Matrin-3 (Ser208). ( A ) The expression of Matrin-3 and phospho-Matrin-3 (Ser208) in NSCs after FGF2 deprivation (-FGF2) and restimulation (+FGF2) on 1D-WB. ( B , C ) Mobility changes in phospho-Matrin-3 (P-Ser208-Matrin-3) and Matrin-3 after FGF2 deprivation and restimulation on 2D-WB. The lanes indicate the FGF2 stimulation times. ( B ) The blue arrowhead indicates the alkaline shift by dephosphorylation. The yellow arrowhead indicates the acidic shift by phosphorylation. ( C ) Red arrowheads indicate phospho-Matrin-3 (P-Ser208-Matrin-3) appearance. ( D ), (a) Expression of Matrin-3, (b) phospho-Matrin-3 (P-Ser208-Matrin-3), and (c) ATM from embrynic and adult mouse cerebra on 1D-WB. ( E ) Immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3), Ki67, and Tuj1 in the E14 mouse cortex (serial sections). CP; cortical plate, IZ; intermediate zone, SVZ; subventricular zone, VZ; ventricular zone. Actin is used as a control. Bar, 100 μm. ( F ) HE, DAPI staining and immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3) of the murine adult hippocampal dentate gyrus (same sections). DG; dentate gyrus, CA; cornet d’Ammon. Bars: 500 μm (upper panel), 200 μm (bottom panel); n = 5. Dotted line, subgranular zone (SGZ).
Figure Legend Snippet: In vitro and in vivo expression of Matrin-3 and phospho-Matrin-3 (Ser208). ( A ) The expression of Matrin-3 and phospho-Matrin-3 (Ser208) in NSCs after FGF2 deprivation (-FGF2) and restimulation (+FGF2) on 1D-WB. ( B , C ) Mobility changes in phospho-Matrin-3 (P-Ser208-Matrin-3) and Matrin-3 after FGF2 deprivation and restimulation on 2D-WB. The lanes indicate the FGF2 stimulation times. ( B ) The blue arrowhead indicates the alkaline shift by dephosphorylation. The yellow arrowhead indicates the acidic shift by phosphorylation. ( C ) Red arrowheads indicate phospho-Matrin-3 (P-Ser208-Matrin-3) appearance. ( D ), (a) Expression of Matrin-3, (b) phospho-Matrin-3 (P-Ser208-Matrin-3), and (c) ATM from embrynic and adult mouse cerebra on 1D-WB. ( E ) Immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3), Ki67, and Tuj1 in the E14 mouse cortex (serial sections). CP; cortical plate, IZ; intermediate zone, SVZ; subventricular zone, VZ; ventricular zone. Actin is used as a control. Bar, 100 μm. ( F ) HE, DAPI staining and immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3) of the murine adult hippocampal dentate gyrus (same sections). DG; dentate gyrus, CA; cornet d’Ammon. Bars: 500 μm (upper panel), 200 μm (bottom panel); n = 5. Dotted line, subgranular zone (SGZ).

Techniques Used: In Vitro, In Vivo, Expressing, De-Phosphorylation Assay, Immunostaining, Staining

Significance of Matrin-3 for maintaining NSCs in vitro and in vivo . ( A ), (a) In vitro Matrin-3-siRNA induces extension of cellular processes of GFP + NSCs. Red arrowheads indicate extension of cellular processes. (b) Matrin-3-siRNA reduced neurosphere-forming stem cells. Bar, 100 μm. (c) The number of GFP + neurospheres per dish was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( B ), (a) Co-immunostaining for GFP and nestin, Ki67, or Tuj1 of NSCs in vitro . Cells are co-immunostained for GFP and nestin, Ki67 (blue arrowheads), or Tuj1 (white arrowheads). (b) Statistical measurements of neuronal differentiation in Matrin-3-knockdown cells. The number of nestin + , Ki67 + and Tuj1 + cells in GFP + cells in one view was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( C ) In utero knockdown of Matrin-3 in the SVZ and VZ layer. (a) GFP + cells in SVZ and VZ areas at E17.5. The cells are counterstained with DAPI. Bar, 500 μm. (b) Immunostaining of Matrin-3 shows the reduced expression in the Matrin-3 siRNA-treated tissue compared to the control siRNA-treated tissue. The dotted line surrounds the electroporated area. (c) HE staining revealed the disordered layer structure. Blue arrowheads indicate the SVZ/VZ area. (d) Co-immunostaining for GFP and nestin, Ki67, or NeuN in the VZ. Yellow arrowheads indicate nestin + , Ki67 + and NeuN + cells in GFP + cells. Bar, 50 μm; n = 4–5.
Figure Legend Snippet: Significance of Matrin-3 for maintaining NSCs in vitro and in vivo . ( A ), (a) In vitro Matrin-3-siRNA induces extension of cellular processes of GFP + NSCs. Red arrowheads indicate extension of cellular processes. (b) Matrin-3-siRNA reduced neurosphere-forming stem cells. Bar, 100 μm. (c) The number of GFP + neurospheres per dish was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( B ), (a) Co-immunostaining for GFP and nestin, Ki67, or Tuj1 of NSCs in vitro . Cells are co-immunostained for GFP and nestin, Ki67 (blue arrowheads), or Tuj1 (white arrowheads). (b) Statistical measurements of neuronal differentiation in Matrin-3-knockdown cells. The number of nestin + , Ki67 + and Tuj1 + cells in GFP + cells in one view was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( C ) In utero knockdown of Matrin-3 in the SVZ and VZ layer. (a) GFP + cells in SVZ and VZ areas at E17.5. The cells are counterstained with DAPI. Bar, 500 μm. (b) Immunostaining of Matrin-3 shows the reduced expression in the Matrin-3 siRNA-treated tissue compared to the control siRNA-treated tissue. The dotted line surrounds the electroporated area. (c) HE staining revealed the disordered layer structure. Blue arrowheads indicate the SVZ/VZ area. (d) Co-immunostaining for GFP and nestin, Ki67, or NeuN in the VZ. Yellow arrowheads indicate nestin + , Ki67 + and NeuN + cells in GFP + cells. Bar, 50 μm; n = 4–5.

Techniques Used: In Vitro, In Vivo, Immunostaining, In Utero, Expressing, Staining

ATM phosphorylates Ser208 of Matrin-3 in the nucleus of NSCs. ( A ) Extension of cellular processes of NSC is induced by ATM inhibition (KU55933) in vitro . The FGF2 deprivation model (-FGF2) is used as an indicator of neuronal differentiation. Red arrowheads indicate extension of cellular processes. ( B ), (a) Phospho-mutant Matrin-3(Ser208Ala) inhibits the formation of neurospheres.P-Ser208-Matrin-3, Flag and DAPI images are merged. Bar, 100 μm. (b) Statistical measurements of Flag + neurospheres in per dish were performed. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( C) , (a) Flag-Matrin-3 and Flag-Matrin-3-Ser208Ala plasmids were transfected into NSCs in vitro . Phospho-mutant Matrin-3 inhibits Matrin-3 nuclear localisation and induces neuronal differentiation. Flag, Tuj1, and DAPI images are merged. Tuj1 + /Flag + cells are observed to assess the nuclear translocation of Matrin-3 and neural differentiation. White arrowheads indicate incidence of Tuj1 + in Flag + cells. (b) The bar graph indicates Tuj1 + /Flag + cells. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( D ) Phosphorylation of Matrin-3 is necessary to regulate NSCs and to maintain self-renewal ability and the undifferentiated state.
Figure Legend Snippet: ATM phosphorylates Ser208 of Matrin-3 in the nucleus of NSCs. ( A ) Extension of cellular processes of NSC is induced by ATM inhibition (KU55933) in vitro . The FGF2 deprivation model (-FGF2) is used as an indicator of neuronal differentiation. Red arrowheads indicate extension of cellular processes. ( B ), (a) Phospho-mutant Matrin-3(Ser208Ala) inhibits the formation of neurospheres.P-Ser208-Matrin-3, Flag and DAPI images are merged. Bar, 100 μm. (b) Statistical measurements of Flag + neurospheres in per dish were performed. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( C) , (a) Flag-Matrin-3 and Flag-Matrin-3-Ser208Ala plasmids were transfected into NSCs in vitro . Phospho-mutant Matrin-3 inhibits Matrin-3 nuclear localisation and induces neuronal differentiation. Flag, Tuj1, and DAPI images are merged. Tuj1 + /Flag + cells are observed to assess the nuclear translocation of Matrin-3 and neural differentiation. White arrowheads indicate incidence of Tuj1 + in Flag + cells. (b) The bar graph indicates Tuj1 + /Flag + cells. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( D ) Phosphorylation of Matrin-3 is necessary to regulate NSCs and to maintain self-renewal ability and the undifferentiated state.

Techniques Used: Inhibition, In Vitro, Mutagenesis, Transfection, Translocation Assay

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    Santa Cruz Biotechnology matrin 3 sirna
    Matrin 3 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology lentivirus particles
    Lentivirus Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology matrin 3 shrna
    Matrin 3 Shrna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology sirna sequences targeting matr3
    Effect of LCH on the expression of <t>Matr3</t> proteins in the oral squamous cell carcinoma cell lines. Pull-down assays of LCH binding to Matr3 ex vivo . Whole (A) HCS2 and (B) HSC3 cell lysates were incubated with Sepharose 4B or LCH-Sepharose 4B beads overnight at 4°C. Immunoblotting of pull-down products was performed with the anti-Matr3 antibody. HSC2 and HSC3 cells were seeded onto a cell culture plate for 24 h and treated with LCH (10, 20 and 30 µM) for 48 h. (C) Cells were harvested and western blot analysis was performed via SDS-PAGE. Expression of Matr3, PARP, cleaved PARP, caspase-3, cleaved caspase-3 and GAPDH were detected by western blot analysis using specific antibodies. GAPDH protein was used as the loading control. LCH, licochalcone H; Matr3, matrin 3; PARP, poly (ADP-ribose) polymerase.
    Sirna Sequences Targeting Matr3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirna sequences targeting matr3/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Santa Cruz Biotechnology mouse matrin 3 sirna
    Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the <t>Matrin-3</t> protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .
    Mouse Matrin 3 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse matrin 3 sirna/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
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    mouse matrin 3 sirna - by Bioz Stars, 2024-04
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    Effect of LCH on the expression of Matr3 proteins in the oral squamous cell carcinoma cell lines. Pull-down assays of LCH binding to Matr3 ex vivo . Whole (A) HCS2 and (B) HSC3 cell lysates were incubated with Sepharose 4B or LCH-Sepharose 4B beads overnight at 4°C. Immunoblotting of pull-down products was performed with the anti-Matr3 antibody. HSC2 and HSC3 cells were seeded onto a cell culture plate for 24 h and treated with LCH (10, 20 and 30 µM) for 48 h. (C) Cells were harvested and western blot analysis was performed via SDS-PAGE. Expression of Matr3, PARP, cleaved PARP, caspase-3, cleaved caspase-3 and GAPDH were detected by western blot analysis using specific antibodies. GAPDH protein was used as the loading control. LCH, licochalcone H; Matr3, matrin 3; PARP, poly (ADP-ribose) polymerase.

    Journal: Oncology Reports

    Article Title: Licochalcone H induces the apoptosis of human oral squamous cell carcinoma cells via regulation of matrin 3

    doi: 10.3892/or.2018.6784

    Figure Lengend Snippet: Effect of LCH on the expression of Matr3 proteins in the oral squamous cell carcinoma cell lines. Pull-down assays of LCH binding to Matr3 ex vivo . Whole (A) HCS2 and (B) HSC3 cell lysates were incubated with Sepharose 4B or LCH-Sepharose 4B beads overnight at 4°C. Immunoblotting of pull-down products was performed with the anti-Matr3 antibody. HSC2 and HSC3 cells were seeded onto a cell culture plate for 24 h and treated with LCH (10, 20 and 30 µM) for 48 h. (C) Cells were harvested and western blot analysis was performed via SDS-PAGE. Expression of Matr3, PARP, cleaved PARP, caspase-3, cleaved caspase-3 and GAPDH were detected by western blot analysis using specific antibodies. GAPDH protein was used as the loading control. LCH, licochalcone H; Matr3, matrin 3; PARP, poly (ADP-ribose) polymerase.

    Article Snippet: siRNA sequences targeting Matr3 (matrin-3 siRNA; cat. no. sc-62604) and a non-targeting control (control siRNA; cat. no. sc-37007) were purchased from Santa Cruz Biotechnology.

    Techniques: Expressing, Binding Assay, Ex Vivo, Incubation, Western Blot, Cell Culture, SDS Page

    Knockdown of the expression of Matr3 results in induction of oral squamous cell carcinoma cell apoptosis. HSC2 and HSC3 cells were transfected with siCon or siMatr3. (A) mRNA expression level of Matr3 in each cell transfected with siCon or siMatr3 was analyzed by reverse transcription-polymerase chain reaction analysis. β-actin was used as a loading control. (B) siCon- or siMatr3-transfected HSC2 and HSC3 cell lysates were determined by western blot analysis using anti-Matr3, anti-caspase-3, anti-cleaved caspase-3, anti-PARP and anti-cleaved PARP antibodies. GAPDH was used as the loading control. (C) HSC2 and HSC3 cells were transfected with siCon or siMatr3 and soft agar assays were performed. Colony numbers and sizes were measured, and the results are expressed as the mean ± standard deviation (*P<0.05) for triplicate experiments. siMatr3, matrin 3-specific targeting siRNA; siCon, scrambled control siRNA; PARP, poly (ADP-ribose) polymerase.

    Journal: Oncology Reports

    Article Title: Licochalcone H induces the apoptosis of human oral squamous cell carcinoma cells via regulation of matrin 3

    doi: 10.3892/or.2018.6784

    Figure Lengend Snippet: Knockdown of the expression of Matr3 results in induction of oral squamous cell carcinoma cell apoptosis. HSC2 and HSC3 cells were transfected with siCon or siMatr3. (A) mRNA expression level of Matr3 in each cell transfected with siCon or siMatr3 was analyzed by reverse transcription-polymerase chain reaction analysis. β-actin was used as a loading control. (B) siCon- or siMatr3-transfected HSC2 and HSC3 cell lysates were determined by western blot analysis using anti-Matr3, anti-caspase-3, anti-cleaved caspase-3, anti-PARP and anti-cleaved PARP antibodies. GAPDH was used as the loading control. (C) HSC2 and HSC3 cells were transfected with siCon or siMatr3 and soft agar assays were performed. Colony numbers and sizes were measured, and the results are expressed as the mean ± standard deviation (*P<0.05) for triplicate experiments. siMatr3, matrin 3-specific targeting siRNA; siCon, scrambled control siRNA; PARP, poly (ADP-ribose) polymerase.

    Article Snippet: siRNA sequences targeting Matr3 (matrin-3 siRNA; cat. no. sc-62604) and a non-targeting control (control siRNA; cat. no. sc-37007) were purchased from Santa Cruz Biotechnology.

    Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Standard Deviation

    Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the Matrin-3 protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .

    Journal: Scientific Reports

    Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells

    doi: 10.1038/s41598-018-31597-x

    Figure Lengend Snippet: Identification of NSC maintaining phosphorylated proteins by 2D-DIGE and mass spectrometry analysis. ( A ) The experimental strategy for 2D-DIGE. ( B ) The 2D-DIGE map and 3D-images of up- or downregulated proteins. The asterisks indicate the up- or downregulated phosphoproteins. The red frame indicates the Matrin-3 protein group. Bottom panels (spots 871 and 889): 3D-images of Matrin-3 protein expression at 0 and 60 min after FGF2 stimulation following 6-h FGF2 deprivation. ( C ) The identified major proteins are listed. The spot numbers correspond to the annotation shown in ( B ). Detailed results of the protein identification are shown in Table .

    Article Snippet: A total of 2 μg of the plasmid encoding EGFP and mouse Matrin-3-siRNA (Santa Cruz Biotechnology, CA, USA) was cotransfected.

    Techniques: Mass Spectrometry, Expressing

    In vitro and in vivo expression of Matrin-3 and phospho-Matrin-3 (Ser208). ( A ) The expression of Matrin-3 and phospho-Matrin-3 (Ser208) in NSCs after FGF2 deprivation (-FGF2) and restimulation (+FGF2) on 1D-WB. ( B , C ) Mobility changes in phospho-Matrin-3 (P-Ser208-Matrin-3) and Matrin-3 after FGF2 deprivation and restimulation on 2D-WB. The lanes indicate the FGF2 stimulation times. ( B ) The blue arrowhead indicates the alkaline shift by dephosphorylation. The yellow arrowhead indicates the acidic shift by phosphorylation. ( C ) Red arrowheads indicate phospho-Matrin-3 (P-Ser208-Matrin-3) appearance. ( D ), (a) Expression of Matrin-3, (b) phospho-Matrin-3 (P-Ser208-Matrin-3), and (c) ATM from embrynic and adult mouse cerebra on 1D-WB. ( E ) Immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3), Ki67, and Tuj1 in the E14 mouse cortex (serial sections). CP; cortical plate, IZ; intermediate zone, SVZ; subventricular zone, VZ; ventricular zone. Actin is used as a control. Bar, 100 μm. ( F ) HE, DAPI staining and immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3) of the murine adult hippocampal dentate gyrus (same sections). DG; dentate gyrus, CA; cornet d’Ammon. Bars: 500 μm (upper panel), 200 μm (bottom panel); n = 5. Dotted line, subgranular zone (SGZ).

    Journal: Scientific Reports

    Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells

    doi: 10.1038/s41598-018-31597-x

    Figure Lengend Snippet: In vitro and in vivo expression of Matrin-3 and phospho-Matrin-3 (Ser208). ( A ) The expression of Matrin-3 and phospho-Matrin-3 (Ser208) in NSCs after FGF2 deprivation (-FGF2) and restimulation (+FGF2) on 1D-WB. ( B , C ) Mobility changes in phospho-Matrin-3 (P-Ser208-Matrin-3) and Matrin-3 after FGF2 deprivation and restimulation on 2D-WB. The lanes indicate the FGF2 stimulation times. ( B ) The blue arrowhead indicates the alkaline shift by dephosphorylation. The yellow arrowhead indicates the acidic shift by phosphorylation. ( C ) Red arrowheads indicate phospho-Matrin-3 (P-Ser208-Matrin-3) appearance. ( D ), (a) Expression of Matrin-3, (b) phospho-Matrin-3 (P-Ser208-Matrin-3), and (c) ATM from embrynic and adult mouse cerebra on 1D-WB. ( E ) Immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3), Ki67, and Tuj1 in the E14 mouse cortex (serial sections). CP; cortical plate, IZ; intermediate zone, SVZ; subventricular zone, VZ; ventricular zone. Actin is used as a control. Bar, 100 μm. ( F ) HE, DAPI staining and immunostaining of phospho-Matrin-3 (P-Ser208-Matrin-3) of the murine adult hippocampal dentate gyrus (same sections). DG; dentate gyrus, CA; cornet d’Ammon. Bars: 500 μm (upper panel), 200 μm (bottom panel); n = 5. Dotted line, subgranular zone (SGZ).

    Article Snippet: A total of 2 μg of the plasmid encoding EGFP and mouse Matrin-3-siRNA (Santa Cruz Biotechnology, CA, USA) was cotransfected.

    Techniques: In Vitro, In Vivo, Expressing, De-Phosphorylation Assay, Immunostaining, Staining

    Significance of Matrin-3 for maintaining NSCs in vitro and in vivo . ( A ), (a) In vitro Matrin-3-siRNA induces extension of cellular processes of GFP + NSCs. Red arrowheads indicate extension of cellular processes. (b) Matrin-3-siRNA reduced neurosphere-forming stem cells. Bar, 100 μm. (c) The number of GFP + neurospheres per dish was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( B ), (a) Co-immunostaining for GFP and nestin, Ki67, or Tuj1 of NSCs in vitro . Cells are co-immunostained for GFP and nestin, Ki67 (blue arrowheads), or Tuj1 (white arrowheads). (b) Statistical measurements of neuronal differentiation in Matrin-3-knockdown cells. The number of nestin + , Ki67 + and Tuj1 + cells in GFP + cells in one view was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( C ) In utero knockdown of Matrin-3 in the SVZ and VZ layer. (a) GFP + cells in SVZ and VZ areas at E17.5. The cells are counterstained with DAPI. Bar, 500 μm. (b) Immunostaining of Matrin-3 shows the reduced expression in the Matrin-3 siRNA-treated tissue compared to the control siRNA-treated tissue. The dotted line surrounds the electroporated area. (c) HE staining revealed the disordered layer structure. Blue arrowheads indicate the SVZ/VZ area. (d) Co-immunostaining for GFP and nestin, Ki67, or NeuN in the VZ. Yellow arrowheads indicate nestin + , Ki67 + and NeuN + cells in GFP + cells. Bar, 50 μm; n = 4–5.

    Journal: Scientific Reports

    Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells

    doi: 10.1038/s41598-018-31597-x

    Figure Lengend Snippet: Significance of Matrin-3 for maintaining NSCs in vitro and in vivo . ( A ), (a) In vitro Matrin-3-siRNA induces extension of cellular processes of GFP + NSCs. Red arrowheads indicate extension of cellular processes. (b) Matrin-3-siRNA reduced neurosphere-forming stem cells. Bar, 100 μm. (c) The number of GFP + neurospheres per dish was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( B ), (a) Co-immunostaining for GFP and nestin, Ki67, or Tuj1 of NSCs in vitro . Cells are co-immunostained for GFP and nestin, Ki67 (blue arrowheads), or Tuj1 (white arrowheads). (b) Statistical measurements of neuronal differentiation in Matrin-3-knockdown cells. The number of nestin + , Ki67 + and Tuj1 + cells in GFP + cells in one view was counted. ** P < 0.01, * P < 0.05 (one-way ANOVA plus Bonferroni/Dunn post-hoc test). Error bars, SE (5 separate experiments). ( C ) In utero knockdown of Matrin-3 in the SVZ and VZ layer. (a) GFP + cells in SVZ and VZ areas at E17.5. The cells are counterstained with DAPI. Bar, 500 μm. (b) Immunostaining of Matrin-3 shows the reduced expression in the Matrin-3 siRNA-treated tissue compared to the control siRNA-treated tissue. The dotted line surrounds the electroporated area. (c) HE staining revealed the disordered layer structure. Blue arrowheads indicate the SVZ/VZ area. (d) Co-immunostaining for GFP and nestin, Ki67, or NeuN in the VZ. Yellow arrowheads indicate nestin + , Ki67 + and NeuN + cells in GFP + cells. Bar, 50 μm; n = 4–5.

    Article Snippet: A total of 2 μg of the plasmid encoding EGFP and mouse Matrin-3-siRNA (Santa Cruz Biotechnology, CA, USA) was cotransfected.

    Techniques: In Vitro, In Vivo, Immunostaining, In Utero, Expressing, Staining

    ATM phosphorylates Ser208 of Matrin-3 in the nucleus of NSCs. ( A ) Extension of cellular processes of NSC is induced by ATM inhibition (KU55933) in vitro . The FGF2 deprivation model (-FGF2) is used as an indicator of neuronal differentiation. Red arrowheads indicate extension of cellular processes. ( B ), (a) Phospho-mutant Matrin-3(Ser208Ala) inhibits the formation of neurospheres.P-Ser208-Matrin-3, Flag and DAPI images are merged. Bar, 100 μm. (b) Statistical measurements of Flag + neurospheres in per dish were performed. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( C) , (a) Flag-Matrin-3 and Flag-Matrin-3-Ser208Ala plasmids were transfected into NSCs in vitro . Phospho-mutant Matrin-3 inhibits Matrin-3 nuclear localisation and induces neuronal differentiation. Flag, Tuj1, and DAPI images are merged. Tuj1 + /Flag + cells are observed to assess the nuclear translocation of Matrin-3 and neural differentiation. White arrowheads indicate incidence of Tuj1 + in Flag + cells. (b) The bar graph indicates Tuj1 + /Flag + cells. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( D ) Phosphorylation of Matrin-3 is necessary to regulate NSCs and to maintain self-renewal ability and the undifferentiated state.

    Journal: Scientific Reports

    Article Title: Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells

    doi: 10.1038/s41598-018-31597-x

    Figure Lengend Snippet: ATM phosphorylates Ser208 of Matrin-3 in the nucleus of NSCs. ( A ) Extension of cellular processes of NSC is induced by ATM inhibition (KU55933) in vitro . The FGF2 deprivation model (-FGF2) is used as an indicator of neuronal differentiation. Red arrowheads indicate extension of cellular processes. ( B ), (a) Phospho-mutant Matrin-3(Ser208Ala) inhibits the formation of neurospheres.P-Ser208-Matrin-3, Flag and DAPI images are merged. Bar, 100 μm. (b) Statistical measurements of Flag + neurospheres in per dish were performed. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( C) , (a) Flag-Matrin-3 and Flag-Matrin-3-Ser208Ala plasmids were transfected into NSCs in vitro . Phospho-mutant Matrin-3 inhibits Matrin-3 nuclear localisation and induces neuronal differentiation. Flag, Tuj1, and DAPI images are merged. Tuj1 + /Flag + cells are observed to assess the nuclear translocation of Matrin-3 and neural differentiation. White arrowheads indicate incidence of Tuj1 + in Flag + cells. (b) The bar graph indicates Tuj1 + /Flag + cells. ** P < 0.01 (Student’s t test). Error bars, SE (5 separate experiments). ( D ) Phosphorylation of Matrin-3 is necessary to regulate NSCs and to maintain self-renewal ability and the undifferentiated state.

    Article Snippet: A total of 2 μg of the plasmid encoding EGFP and mouse Matrin-3-siRNA (Santa Cruz Biotechnology, CA, USA) was cotransfected.

    Techniques: Inhibition, In Vitro, Mutagenesis, Transfection, Translocation Assay