matrin 3 sirna  (Santa Cruz Biotechnology)

Journal: Oncology Reports

Article Title: Licochalcone H induces the apoptosis of human oral squamous cell carcinoma cells via regulation of matrin 3

doi: 10.3892/or.2018.6784

Figure Lengend Snippet: Effect of LCH on the expression of Matr3 proteins in the oral squamous cell carcinoma cell lines. Pull-down assays of LCH binding to Matr3 ex vivo . Whole (A) HCS2 and (B) HSC3 cell lysates were incubated with Sepharose 4B or LCH-Sepharose 4B beads overnight at 4°C. Immunoblotting of pull-down products was performed with the anti-Matr3 antibody. HSC2 and HSC3 cells were seeded onto a cell culture plate for 24 h and treated with LCH (10, 20 and 30 µM) for 48 h. (C) Cells were harvested and western blot analysis was performed via SDS-PAGE. Expression of Matr3, PARP, cleaved PARP, caspase-3, cleaved caspase-3 and GAPDH were detected by western blot analysis using specific antibodies. GAPDH protein was used as the loading control. LCH, licochalcone H; Matr3, matrin 3; PARP, poly (ADP-ribose) polymerase.

Article Snippet: siRNA sequences targeting Matr3 (matrin-3 siRNA; cat. no. sc-62604) and a non-targeting control (control siRNA; cat. no. sc-37007) were purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Binding Assay, Ex Vivo, Incubation, Western Blot, Cell Culture, SDS Page

Journal: Oncology Reports

Article Title: Licochalcone H induces the apoptosis of human oral squamous cell carcinoma cells via regulation of matrin 3

doi: 10.3892/or.2018.6784

Figure Lengend Snippet: Knockdown of the expression of Matr3 results in induction of oral squamous cell carcinoma cell apoptosis. HSC2 and HSC3 cells were transfected with siCon or siMatr3. (A) mRNA expression level of Matr3 in each cell transfected with siCon or siMatr3 was analyzed by reverse transcription-polymerase chain reaction analysis. β-actin was used as a loading control. (B) siCon- or siMatr3-transfected HSC2 and HSC3 cell lysates were determined by western blot analysis using anti-Matr3, anti-caspase-3, anti-cleaved caspase-3, anti-PARP and anti-cleaved PARP antibodies. GAPDH was used as the loading control. (C) HSC2 and HSC3 cells were transfected with siCon or siMatr3 and soft agar assays were performed. Colony numbers and sizes were measured, and the results are expressed as the mean ± standard deviation (*P<0.05) for triplicate experiments. siMatr3, matrin 3-specific targeting siRNA; siCon, scrambled control siRNA; PARP, poly (ADP-ribose) polymerase.

Article Snippet: siRNA sequences targeting Matr3 (matrin-3 siRNA; cat. no. sc-62604) and a non-targeting control (control siRNA; cat. no. sc-37007) were purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Standard Deviation