materials concanavalin a  (Millipore)

 
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    Name:
    Concanavalin A from Canavalia ensiformis
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    Catalog Number:
    C2631
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    Structured Review

    Millipore materials concanavalin a
    Effect of SCG on protein expression of TRAIL, DR5, FASL, FAS, IL-33, and caspase-3 in Con A-treated mice.  (A)  The expression of TRAIL.  (B)  The expression of DR5.  (C)  The expression of IL-33.  (D)  The expression of FASL.  (E)  The expression of FAS.  (F)  The expression of cleaved caspase-3. Data were expressed as mean ±  SD  ( n  = 3).  ∗∗ P

    https://www.bioz.com/result/materials concanavalin a/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    materials concanavalin a - by Bioz Stars, 2021-04
    99/100 stars

    Images

    1) Product Images from "Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration"

    Article Title: Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2018.00624

    Effect of SCG on protein expression of TRAIL, DR5, FASL, FAS, IL-33, and caspase-3 in Con A-treated mice.  (A)  The expression of TRAIL.  (B)  The expression of DR5.  (C)  The expression of IL-33.  (D)  The expression of FASL.  (E)  The expression of FAS.  (F)  The expression of cleaved caspase-3. Data were expressed as mean ±  SD  ( n  = 3).  ∗∗ P
    Figure Legend Snippet: Effect of SCG on protein expression of TRAIL, DR5, FASL, FAS, IL-33, and caspase-3 in Con A-treated mice. (A) The expression of TRAIL. (B) The expression of DR5. (C) The expression of IL-33. (D) The expression of FASL. (E) The expression of FAS. (F) The expression of cleaved caspase-3. Data were expressed as mean ± SD ( n = 3). ∗∗ P

    Techniques Used: Expressing, Mouse Assay

    The summarized action pathway of SCG on Con A-induced liver injury.
    Figure Legend Snippet: The summarized action pathway of SCG on Con A-induced liver injury.

    Techniques Used:

    Effect of SCG on the levels of inflammatory cytokines in Con A-induced liver injury.  (A)  The expression level of IFN-γ.  (B)  The expression level of TNF-α.  (C)  The expression level of IL-2.  (D)  The expression of IL-4.  (E)  The expression level of IL-5. Data were expressed as mean ± SD  ( n  = 6).  ∗∗ P
    Figure Legend Snippet: Effect of SCG on the levels of inflammatory cytokines in Con A-induced liver injury. (A) The expression level of IFN-γ. (B) The expression level of TNF-α. (C) The expression level of IL-2. (D) The expression of IL-4. (E) The expression level of IL-5. Data were expressed as mean ± SD ( n = 6). ∗∗ P

    Techniques Used: Expressing

    Pharmacological effect of SCG on Con A-induced liver injury in mice.  (A)  The serum level of ALT.  (B)  The serum level of AST.  (C)  The activity of MPO.  (D)  HE-stained liver section for histopathology evaluations and the histological changes. Data were expressed as mean ±  SD  ( n  = 6).  ∗∗ P
    Figure Legend Snippet: Pharmacological effect of SCG on Con A-induced liver injury in mice. (A) The serum level of ALT. (B) The serum level of AST. (C) The activity of MPO. (D) HE-stained liver section for histopathology evaluations and the histological changes. Data were expressed as mean ± SD ( n = 6). ∗∗ P

    Techniques Used: Mouse Assay, AST Assay, Activity Assay, Staining, Histopathology

    Effect of SCG on hepatocyte apoptosis in Con A-treated mice.
    Figure Legend Snippet: Effect of SCG on hepatocyte apoptosis in Con A-treated mice.

    Techniques Used: Mouse Assay

    Effect of SCG on mRNA expression of TRAIL, FASL, IL-33, and caspase-3 in Con A-treated mice.  (A)  The mRNA level of TRAIL.  (B)  The mRNA level of FASL.  (C)  The mRNA level of IL-33.  (D)  The mRNA level of caspase-3. Data were expressed as mean ± SD  ( n  = 6).  ∗∗ P
    Figure Legend Snippet: Effect of SCG on mRNA expression of TRAIL, FASL, IL-33, and caspase-3 in Con A-treated mice. (A) The mRNA level of TRAIL. (B) The mRNA level of FASL. (C) The mRNA level of IL-33. (D) The mRNA level of caspase-3. Data were expressed as mean ± SD ( n = 6). ∗∗ P

    Techniques Used: Expressing, Mouse Assay

    Related Articles

    Incubation:

    Article Title: A trivalent Apx-fusion protein delivered by E. coli outer membrane vesicles induce protection against Actinobacillus pleuropneumoniae of serotype 1 and 7 challenge in a murine model
    Article Snippet: .. The cells were stimulated with Apxr-OMVs (5 μg/ well) or concanavalin A (ConA) (SIGMA-ALDRICH, USA), then incubated for 72 h at 37°C in a 5% CO2 incubator. .. Lymphocyte proliferation assay was performed using MTS Cell Proliferation Assay Kit (BEYOTIME, China), following the manufacturer’s instructions.

    Article Title: Limited overlapping roles of P15INK4b and P18INK4c cell cycle inhibitors in proliferation and tumorigenesis
    Article Snippet: .. For in vitro proliferation assays, duplicate cultures containing 2 × 105 spleen cells were incubated in 200 µl of RPMI-1640 medium supplemented with 10% FBS in either the presence or absence of 10 µg/ml concanavalin A (Sigma) or 50 ng/ml PMA (Sigma) + 500 ng/ml of ionomycin (Sigma). .. After 3 days in culture, cells were pulsed with 10 µM BrdU for 8 h. Labeled cells were harvested and BrdU incorporation measured in a flow cytometer as indicated above.

    In Vitro:

    Article Title: Limited overlapping roles of P15INK4b and P18INK4c cell cycle inhibitors in proliferation and tumorigenesis
    Article Snippet: .. For in vitro proliferation assays, duplicate cultures containing 2 × 105 spleen cells were incubated in 200 µl of RPMI-1640 medium supplemented with 10% FBS in either the presence or absence of 10 µg/ml concanavalin A (Sigma) or 50 ng/ml PMA (Sigma) + 500 ng/ml of ionomycin (Sigma). .. After 3 days in culture, cells were pulsed with 10 µM BrdU for 8 h. Labeled cells were harvested and BrdU incorporation measured in a flow cytometer as indicated above.

    Mouse Assay:

    Article Title: Hepatocyte Toll-Like Receptor 5 Promotes Bacterial Clearance and Protects Mice Against High-Fat Diet–Induced Liver Disease
    Article Snippet: Mice then were euthanized, and colon length, colon weight, spleen weight, liver, and adipose tissue weights were measured. .. Concanavalin A–Induced Hepatitis Eight-week-old mice were injected intravenously (tail vein) with Concanavalin A (ConA) (Sigma), 15 mg/kg of body weight, diluted in sterile pyrogen-free PBS, as previously described. .. Twenty-four hours after injection, blood was collected by retro-orbital capillary plexus and hemolysis-free serum was generated by centrifugation of blood using serum separator tubes (Becton Dickinson).

    Injection:

    Article Title: Hepatocyte Toll-Like Receptor 5 Promotes Bacterial Clearance and Protects Mice Against High-Fat Diet–Induced Liver Disease
    Article Snippet: Mice then were euthanized, and colon length, colon weight, spleen weight, liver, and adipose tissue weights were measured. .. Concanavalin A–Induced Hepatitis Eight-week-old mice were injected intravenously (tail vein) with Concanavalin A (ConA) (Sigma), 15 mg/kg of body weight, diluted in sterile pyrogen-free PBS, as previously described. .. Twenty-four hours after injection, blood was collected by retro-orbital capillary plexus and hemolysis-free serum was generated by centrifugation of blood using serum separator tubes (Becton Dickinson).

    Cell Culture:

    Article Title: Yersinia pestis Antigen F1 but Not LcrV Induced Humoral and Cellular Immune Responses in Humans Immunized with Live Plague Vaccine—Comparison of Immunoinformatic and Immunological Approaches
    Article Snippet: Briefly, PBMCs were isolated from heparinized blood by density gradient centrifugation according to a standard protocol and seeded immediately at a concentration of 105 cells per well. .. Cells were cultured in Dulbecco’s modified Eagle medium (DMEM)/F12 medium supplemented with 10% fetal bovine serum (FBS) and antibiotic–antimycotic solution in 96-well plates for 6 days with or without recombinant F1 or LcrV (both at 5 μg/mL) or concanavalin A (Sigma, North Liberty, IA, USA) at 1 μg/mL, used as a positive stimulation control. .. The proliferative response was measured using a Cell Proliferation ELISA, BrdU chemiluminescent kit (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s protocol.

    Modification:

    Article Title: Yersinia pestis Antigen F1 but Not LcrV Induced Humoral and Cellular Immune Responses in Humans Immunized with Live Plague Vaccine—Comparison of Immunoinformatic and Immunological Approaches
    Article Snippet: Briefly, PBMCs were isolated from heparinized blood by density gradient centrifugation according to a standard protocol and seeded immediately at a concentration of 105 cells per well. .. Cells were cultured in Dulbecco’s modified Eagle medium (DMEM)/F12 medium supplemented with 10% fetal bovine serum (FBS) and antibiotic–antimycotic solution in 96-well plates for 6 days with or without recombinant F1 or LcrV (both at 5 μg/mL) or concanavalin A (Sigma, North Liberty, IA, USA) at 1 μg/mL, used as a positive stimulation control. .. The proliferative response was measured using a Cell Proliferation ELISA, BrdU chemiluminescent kit (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s protocol.

    Recombinant:

    Article Title: Yersinia pestis Antigen F1 but Not LcrV Induced Humoral and Cellular Immune Responses in Humans Immunized with Live Plague Vaccine—Comparison of Immunoinformatic and Immunological Approaches
    Article Snippet: Briefly, PBMCs were isolated from heparinized blood by density gradient centrifugation according to a standard protocol and seeded immediately at a concentration of 105 cells per well. .. Cells were cultured in Dulbecco’s modified Eagle medium (DMEM)/F12 medium supplemented with 10% fetal bovine serum (FBS) and antibiotic–antimycotic solution in 96-well plates for 6 days with or without recombinant F1 or LcrV (both at 5 μg/mL) or concanavalin A (Sigma, North Liberty, IA, USA) at 1 μg/mL, used as a positive stimulation control. .. The proliferative response was measured using a Cell Proliferation ELISA, BrdU chemiluminescent kit (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s protocol.

    Microscopy:

    Article Title: Translational regulation of Pmt1 and Pmt2 by Bfr1 affects unfolded protein O-mannosylation
    Article Snippet: Plates were incubated at 30°C for 4 h for cells to reach the logarithmic growth phase. .. Cultures were then transferred by the liquid handler into glass-bottom 384-well microscope plates (Matrical Bioscience; Spokane, WA, USA) coated with Concanavalin A (Sigma-Aldrich). ..

    Droplet Countercurrent Chromatography:

    Article Title: Ultrasensitive impedimetric lectin biosensors with efficient antifouling properties applied in glycoprofiling of human serum samples
    Article Snippet: .. 11-mercaptoundecanoic acid (MUA), potassium hexacyanoferrate(III), potassium hexacyanoferrate(II) trihydrate, sodium chroride, potassium chloride, 1,3-propane sultone, N-hydroxysuccinimide (NHS), N -(3-dimethylaminopropyl)- N ′-ethylcarbodiimide hydrochloride (EDC), sodium sulphate, N , N -dimethylethylene diamine, dicyclohexylcarbdiimide (DCC), sodium periodate, ethylene glycol, ethanolamine, acetonitrile, dichloromethane, tetrahydrofuran (THF), glycine, hydrochloric acid, Tween 20, fetuin (FET, 8.7% of N-acetylneuraminic acid), asialofetuin (ASF, ≤ 0.5% of N-acetylneuraminic acid), invertase from baker´s yeasts (INV), transferrin (TRF), Ricinus communis agglutinin ( handle with special care since it is a toxin ) and concanavalin A were purchased from Sigma Aldrich (USA). ( R )-Lipoic acid was purchased from TCI Europe. .. SNA I lectin from Sambucus nigra was purchased from Gentaur (Belgium).

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    Millipore materials concanavalin a
    Effect of SCG on protein expression of TRAIL, DR5, FASL, FAS, IL-33, and caspase-3 in Con A-treated mice.  (A)  The expression of TRAIL.  (B)  The expression of DR5.  (C)  The expression of IL-33.  (D)  The expression of FASL.  (E)  The expression of FAS.  (F)  The expression of cleaved caspase-3. Data were expressed as mean ±  SD  ( n  = 3).  ∗∗ P
    Materials Concanavalin A, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/materials concanavalin a/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    materials concanavalin a - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    93
    Millipore methods concanavalin a iii
    Effect of SCG on protein expression of TRAIL, DR5, FASL, FAS, IL-33, and caspase-3 in Con A-treated mice.  (A)  The expression of TRAIL.  (B)  The expression of DR5.  (C)  The expression of IL-33.  (D)  The expression of FASL.  (E)  The expression of FAS.  (F)  The expression of cleaved caspase-3. Data were expressed as mean ±  SD  ( n  = 3).  ∗∗ P
    Methods Concanavalin A Iii, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/methods concanavalin a iii/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    methods concanavalin a iii - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Effect of SCG on protein expression of TRAIL, DR5, FASL, FAS, IL-33, and caspase-3 in Con A-treated mice.  (A)  The expression of TRAIL.  (B)  The expression of DR5.  (C)  The expression of IL-33.  (D)  The expression of FASL.  (E)  The expression of FAS.  (F)  The expression of cleaved caspase-3. Data were expressed as mean ±  SD  ( n  = 3).  ∗∗ P

    Journal: Frontiers in Pharmacology

    Article Title: Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration

    doi: 10.3389/fphar.2018.00624

    Figure Lengend Snippet: Effect of SCG on protein expression of TRAIL, DR5, FASL, FAS, IL-33, and caspase-3 in Con A-treated mice. (A) The expression of TRAIL. (B) The expression of DR5. (C) The expression of IL-33. (D) The expression of FASL. (E) The expression of FAS. (F) The expression of cleaved caspase-3. Data were expressed as mean ± SD ( n = 3). ∗∗ P

    Article Snippet: Materials Concanavalin A was purchased from Sigma (St Louis, MO, United States).

    Techniques: Expressing, Mouse Assay

    The summarized action pathway of SCG on Con A-induced liver injury.

    Journal: Frontiers in Pharmacology

    Article Title: Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration

    doi: 10.3389/fphar.2018.00624

    Figure Lengend Snippet: The summarized action pathway of SCG on Con A-induced liver injury.

    Article Snippet: Materials Concanavalin A was purchased from Sigma (St Louis, MO, United States).

    Techniques:

    Effect of SCG on the levels of inflammatory cytokines in Con A-induced liver injury.  (A)  The expression level of IFN-γ.  (B)  The expression level of TNF-α.  (C)  The expression level of IL-2.  (D)  The expression of IL-4.  (E)  The expression level of IL-5. Data were expressed as mean ± SD  ( n  = 6).  ∗∗ P

    Journal: Frontiers in Pharmacology

    Article Title: Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration

    doi: 10.3389/fphar.2018.00624

    Figure Lengend Snippet: Effect of SCG on the levels of inflammatory cytokines in Con A-induced liver injury. (A) The expression level of IFN-γ. (B) The expression level of TNF-α. (C) The expression level of IL-2. (D) The expression of IL-4. (E) The expression level of IL-5. Data were expressed as mean ± SD ( n = 6). ∗∗ P

    Article Snippet: Materials Concanavalin A was purchased from Sigma (St Louis, MO, United States).

    Techniques: Expressing

    Pharmacological effect of SCG on Con A-induced liver injury in mice.  (A)  The serum level of ALT.  (B)  The serum level of AST.  (C)  The activity of MPO.  (D)  HE-stained liver section for histopathology evaluations and the histological changes. Data were expressed as mean ±  SD  ( n  = 6).  ∗∗ P

    Journal: Frontiers in Pharmacology

    Article Title: Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration

    doi: 10.3389/fphar.2018.00624

    Figure Lengend Snippet: Pharmacological effect of SCG on Con A-induced liver injury in mice. (A) The serum level of ALT. (B) The serum level of AST. (C) The activity of MPO. (D) HE-stained liver section for histopathology evaluations and the histological changes. Data were expressed as mean ± SD ( n = 6). ∗∗ P

    Article Snippet: Materials Concanavalin A was purchased from Sigma (St Louis, MO, United States).

    Techniques: Mouse Assay, AST Assay, Activity Assay, Staining, Histopathology

    Effect of SCG on hepatocyte apoptosis in Con A-treated mice.

    Journal: Frontiers in Pharmacology

    Article Title: Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration

    doi: 10.3389/fphar.2018.00624

    Figure Lengend Snippet: Effect of SCG on hepatocyte apoptosis in Con A-treated mice.

    Article Snippet: Materials Concanavalin A was purchased from Sigma (St Louis, MO, United States).

    Techniques: Mouse Assay

    Effect of SCG on mRNA expression of TRAIL, FASL, IL-33, and caspase-3 in Con A-treated mice.  (A)  The mRNA level of TRAIL.  (B)  The mRNA level of FASL.  (C)  The mRNA level of IL-33.  (D)  The mRNA level of caspase-3. Data were expressed as mean ± SD  ( n  = 6).  ∗∗ P

    Journal: Frontiers in Pharmacology

    Article Title: Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration

    doi: 10.3389/fphar.2018.00624

    Figure Lengend Snippet: Effect of SCG on mRNA expression of TRAIL, FASL, IL-33, and caspase-3 in Con A-treated mice. (A) The mRNA level of TRAIL. (B) The mRNA level of FASL. (C) The mRNA level of IL-33. (D) The mRNA level of caspase-3. Data were expressed as mean ± SD ( n = 6). ∗∗ P

    Article Snippet: Materials Concanavalin A was purchased from Sigma (St Louis, MO, United States).

    Techniques: Expressing, Mouse Assay

    This is a schematic representation of the aggregative nature used in the Con-A/dendrimer assay. With the assay exposed to low glucose concentrations (A) the assay emits lower fluorescence than when the assay is exposed to high glucose concentrations (B).

    Journal: Biomedical Optics Express

    Article Title: Encapsulation of a Concanavalin A/dendrimer glucose sensing assay within microporated poly (ethylene glycol) microspheres

    doi: 10.1364/BOE.2.001243

    Figure Lengend Snippet: This is a schematic representation of the aggregative nature used in the Con-A/dendrimer assay. With the assay exposed to low glucose concentrations (A) the assay emits lower fluorescence than when the assay is exposed to high glucose concentrations (B).

    Article Snippet: Materials and methods Concanavalin A (type IV), dimethyl sulfoxide (DMSO), trizma- hydrochloride (Tris-HCl), manganese chloride (MnCl2), sodium chloride (NaCl), D-glucose, D-mannitol, poly(ethylene glycol) diacrylate (PEG-DA) (Mn ~575), and light mineral oil were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Fluorescence

    These are hybrid images of false-colored confocal fluorescence slices overlaid onto bright-field images of the microspheres. The fluorescence displays the location of the labeled Con-A within the hydrogel, and it shows the differences of the pore distribution between PEG50 microspheres (A) and microporated microspheres (B). The large pores in B allow for the sensing mechanism to function effectively while maintaining reversibility by preventing leaching. The reference bar is 100 μm in length.

    Journal: Biomedical Optics Express

    Article Title: Encapsulation of a Concanavalin A/dendrimer glucose sensing assay within microporated poly (ethylene glycol) microspheres

    doi: 10.1364/BOE.2.001243

    Figure Lengend Snippet: These are hybrid images of false-colored confocal fluorescence slices overlaid onto bright-field images of the microspheres. The fluorescence displays the location of the labeled Con-A within the hydrogel, and it shows the differences of the pore distribution between PEG50 microspheres (A) and microporated microspheres (B). The large pores in B allow for the sensing mechanism to function effectively while maintaining reversibility by preventing leaching. The reference bar is 100 μm in length.

    Article Snippet: Materials and methods Concanavalin A (type IV), dimethyl sulfoxide (DMSO), trizma- hydrochloride (Tris-HCl), manganese chloride (MnCl2), sodium chloride (NaCl), D-glucose, D-mannitol, poly(ethylene glycol) diacrylate (PEG-DA) (Mn ~575), and light mineral oil were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Fluorescence, Labeling

    Leaching Studies: Steady state fluorescent scans were taken for microporated and PEG50 microspheres at 0 mg/dL and 600 mg/dL. The supernatant was removed and scanned to determine if labeled Con-A leached out of the matrix. Fluorescence data is plotted relative to the initial 0 mg/dL scan. Error bars depict the standard deviation of the recorded measurements.

    Journal: Biomedical Optics Express

    Article Title: Encapsulation of a Concanavalin A/dendrimer glucose sensing assay within microporated poly (ethylene glycol) microspheres

    doi: 10.1364/BOE.2.001243

    Figure Lengend Snippet: Leaching Studies: Steady state fluorescent scans were taken for microporated and PEG50 microspheres at 0 mg/dL and 600 mg/dL. The supernatant was removed and scanned to determine if labeled Con-A leached out of the matrix. Fluorescence data is plotted relative to the initial 0 mg/dL scan. Error bars depict the standard deviation of the recorded measurements.

    Article Snippet: Materials and methods Concanavalin A (type IV), dimethyl sulfoxide (DMSO), trizma- hydrochloride (Tris-HCl), manganese chloride (MnCl2), sodium chloride (NaCl), D-glucose, D-mannitol, poly(ethylene glycol) diacrylate (PEG-DA) (Mn ~575), and light mineral oil were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Labeling, Fluorescence, Standard Deviation

    CD Spectra of Globular Soluble Proteins. CD spectra of soluble globular proteins representing the four CATH classes (mostly helical: panels A, B, and C; mostly sheet: panels D, E, and F; mixed helix/sheet: panels G and H; and irregular: panel I). Solid lines represent solution spectra, while dashed lines are from films. The error bars indicate one standard deviation in three repeated measurements. The proteins are A: hemoglobin, B: myoglobin, C: cytochrome C, D: α‐chymotrypsin, E: elastase, F: concanavalin A, G: ribonuclease A, H: carbonic anhydrase II, I: aprotinin.

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Differential dehydration effects on globular proteins and intrinsically disordered proteins during film formation

    doi: 10.1002/pro.3118

    Figure Lengend Snippet: CD Spectra of Globular Soluble Proteins. CD spectra of soluble globular proteins representing the four CATH classes (mostly helical: panels A, B, and C; mostly sheet: panels D, E, and F; mixed helix/sheet: panels G and H; and irregular: panel I). Solid lines represent solution spectra, while dashed lines are from films. The error bars indicate one standard deviation in three repeated measurements. The proteins are A: hemoglobin, B: myoglobin, C: cytochrome C, D: α‐chymotrypsin, E: elastase, F: concanavalin A, G: ribonuclease A, H: carbonic anhydrase II, I: aprotinin.

    Article Snippet: Materials and Methods Concanavalin A (Canavalia ensiformis ) [CON], carbonic anhydrase II (Bos taurus ) [CAH], elastase (Sus scrofa ) [ELA], bovine trypsin inhibitor (aprotinin) [APO], α‐chymotrypsin (Bos taurus ) [CHM], cytochrome C (Equus caballus ) [CYC], were purchased from Sigma.

    Techniques: Standard Deviation

    Effect of Rehydration on the SRCD Spectra of Protein Films. Black solid lines are dehydrated films, gray solid lines are resolubilized films, and dashed lines are HT spectra in the same respective colors as Figure 2. (A) Concanavalin A (globular) and (B) β‐synuclein (IDP).

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Differential dehydration effects on globular proteins and intrinsically disordered proteins during film formation

    doi: 10.1002/pro.3118

    Figure Lengend Snippet: Effect of Rehydration on the SRCD Spectra of Protein Films. Black solid lines are dehydrated films, gray solid lines are resolubilized films, and dashed lines are HT spectra in the same respective colors as Figure 2. (A) Concanavalin A (globular) and (B) β‐synuclein (IDP).

    Article Snippet: Materials and Methods Concanavalin A (Canavalia ensiformis ) [CON], carbonic anhydrase II (Bos taurus ) [CAH], elastase (Sus scrofa ) [ELA], bovine trypsin inhibitor (aprotinin) [APO], α‐chymotrypsin (Bos taurus ) [CHM], cytochrome C (Equus caballus ) [CYC], were purchased from Sigma.

    Techniques: