materials cinnamic aldehyde  (Millipore)


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    • 99
    Name:
    trans Cinnamaldehyde
    Description:
    The neuroprotective and anti inflammatory effects of trans cinnamaldehyde TCA were studied
    Catalog Number:
    239968
    Price:
    None
    Applications:
    trans-Cinnamaldehyde was used to study the useful amino groups at aminosilanized surface.
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    Structured Review

    Millipore materials cinnamic aldehyde
    trans Cinnamaldehyde
    The neuroprotective and anti inflammatory effects of trans cinnamaldehyde TCA were studied
    https://www.bioz.com/result/materials cinnamic aldehyde/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    materials cinnamic aldehyde - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis
    Article Snippet: .. For flow cytometry experiments, treated cells were fixed in 4% formalin for 15 min and stained with conformation-specific primary antibodies (Bax 6A7 [Sigma, no. B8429] and Bak NT [Millipore, no. 06536]). .. Species-specific Alexa fluor 647 or Cy5-conjugated secondary antibodies were used to detect activated Bax and Bak by flow cytometry.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: A Requirement for Bid for Induction of Apoptosis by Photodynamic Therapy with a Lysosome- but not a Mitochondrion-Targeted Photosensitizer
    Article Snippet: .. After transfer to PVDF membranes, the proteins were probed with one or more of the following antibodies: anti-Bax (1:500 dilution, cat no. N-20, Santa Cruz, Santa Cruz, CA), anti-Bak (1:1000 dilution, cat no.06536, Millipore, Billerica, MA), anti-Bid (1:500 dilution, cat no. 550365, BD Biosciences, San Diego, CA), anti-active caspase-3 (1:1000 dilution, cat. No. 559565, BD Pharmingen, San Diego, CA) or anti-actin (1:2000 dilution, cat.no. .. MS-1295, NeoMarker, Fremont, CA).

    Flow Cytometry:

    Article Title: Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis
    Article Snippet: .. For flow cytometry experiments, treated cells were fixed in 4% formalin for 15 min and stained with conformation-specific primary antibodies (Bax 6A7 [Sigma, no. B8429] and Bak NT [Millipore, no. 06536]). .. Species-specific Alexa fluor 647 or Cy5-conjugated secondary antibodies were used to detect activated Bax and Bak by flow cytometry.

    Cytometry:

    Article Title: Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis
    Article Snippet: .. For flow cytometry experiments, treated cells were fixed in 4% formalin for 15 min and stained with conformation-specific primary antibodies (Bax 6A7 [Sigma, no. B8429] and Bak NT [Millipore, no. 06536]). .. Species-specific Alexa fluor 647 or Cy5-conjugated secondary antibodies were used to detect activated Bax and Bak by flow cytometry.

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    • anti bak  (Millipore)
    98
    Millipore anti bak
    Western blot analysis of the expression of Bid, <t>Bax,</t> <t>Bak,</t> and actin (as loading control) in Bid +/+ and Bid −/− cells. Equal amounts of protein from whole cell lysates were resolved on SDS-PAGE and processed for western blot analysis
    Anti Bak, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bak/product/Millipore
    Average 98 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    anti bak - by Bioz Stars, 2021-01
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    Western blot analysis of the expression of Bid, Bax, Bak, and actin (as loading control) in Bid +/+ and Bid −/− cells. Equal amounts of protein from whole cell lysates were resolved on SDS-PAGE and processed for western blot analysis

    Journal: Photochemistry and photobiology

    Article Title: A Requirement for Bid for Induction of Apoptosis by Photodynamic Therapy with a Lysosome- but not a Mitochondrion-Targeted Photosensitizer

    doi: 10.1111/j.1751-1097.2010.00766.x

    Figure Lengend Snippet: Western blot analysis of the expression of Bid, Bax, Bak, and actin (as loading control) in Bid +/+ and Bid −/− cells. Equal amounts of protein from whole cell lysates were resolved on SDS-PAGE and processed for western blot analysis

    Article Snippet: After transfer to PVDF membranes, the proteins were probed with one or more of the following antibodies: anti-Bax (1:500 dilution, cat no. N-20, Santa Cruz, Santa Cruz, CA), anti-Bak (1:1000 dilution, cat no.06536, Millipore, Billerica, MA), anti-Bid (1:500 dilution, cat no. 550365, BD Biosciences, San Diego, CA), anti-active caspase-3 (1:1000 dilution, cat. No. 559565, BD Pharmingen, San Diego, CA) or anti-actin (1:2000 dilution, cat.no.

    Techniques: Western Blot, Expressing, SDS Page

    Bax is necessary for β-cell apoptosis induced by Pdx1 suppression. A , Bax protein levels in Bax KD cells. 3 days after Bax knockdown, immunoblot of Bax, Bak, and actin in Bax KD MIN6 cells. B , Bax KD inhibits the translocation of cytochrome c in Pdx1/Bax DKD MIN6 cells. Fluorescence microscopy of MIN6 cells 4 days after exposure to Pdx1 and Bax lentivirus. Green represents cytochrome c immunostaining, and blue is DAPI staining. Red arrows indicate apoptotic cells that have lost cytochrome c . Scale bar, 20 μm. C , Bax inhibits cytochrome c release induced by Pdx1 KD. 3 days after Bax and Pdx1 KD in MIN6 cells, the levels of cytochrome c in the cytosol and mitochondria fractions are determined by immunoblot. Bar graphs represent quantification using densitometry of the relative amounts of the indicated proteins determined by Western blots ( n = 3). *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Pancreatic β-Cell Death due to Pdx-1 Deficiency Requires Multi-BH Domain Protein Bax but Not Bak *

    doi: 10.1074/jbc.M115.705293

    Figure Lengend Snippet: Bax is necessary for β-cell apoptosis induced by Pdx1 suppression. A , Bax protein levels in Bax KD cells. 3 days after Bax knockdown, immunoblot of Bax, Bak, and actin in Bax KD MIN6 cells. B , Bax KD inhibits the translocation of cytochrome c in Pdx1/Bax DKD MIN6 cells. Fluorescence microscopy of MIN6 cells 4 days after exposure to Pdx1 and Bax lentivirus. Green represents cytochrome c immunostaining, and blue is DAPI staining. Red arrows indicate apoptotic cells that have lost cytochrome c . Scale bar, 20 μm. C , Bax inhibits cytochrome c release induced by Pdx1 KD. 3 days after Bax and Pdx1 KD in MIN6 cells, the levels of cytochrome c in the cytosol and mitochondria fractions are determined by immunoblot. Bar graphs represent quantification using densitometry of the relative amounts of the indicated proteins determined by Western blots ( n = 3). *, p

    Article Snippet: The blots were probed with antibodies against Pdx1 (07-696; Millipore), cytochrome c (mouse6H2.B4, Millipore), Actin (A-2066; Sigma), Bax (6A7) (2281-MC-100; Trevigen), Bax (N20) (SC493, Santa Cruz Biotechnology) and Bak (06536, Millipore).

    Techniques: Translocation Assay, Fluorescence, Microscopy, Immunostaining, Staining, Western Blot

    Pdx1 knockdown induces Bax activation in MIN6 cells and islets. A , Bax and Bak mRNA levels in control and Pdx1 KD cells. 3 days after Pdx1 KD in MIN6 cells, Bax and Bak mRNA levels were not different between control and Pdx1 KD cells ( n = 3). B , Western blot of Pdx1 KD cells. 3 days after Pdx1 KD in MIN6 cells, immunoblot analysis was performed to determine Pdx1, Bax, and Bak in Pdx1 KD MIN6 cells. C , Bax nuclear/cytosolic translocation in Pdx1 KD MIN6 cells. 3 days after Pdx1 shRNA lentivirus infection, cytosolic and nuclear proteins were analyzed by Western blot. D , immunoprecipitate of Bax. 3 days after Pdx1 KD in MIN6 cells, cells were lysed in 1% CHAPS and then immunoprecipitated with the 6A7 anti-BAX antibody. Immunoprecipitates were analyzed by anti-BAX (N20) immunoblots. E , effects of Pdx1 on ΔΨ m . MIn6 cells were treated with Pdx1 shRNA lentivirus for 0, 4 days. Cells were stained with TMRE dye to measure ΔΨ m . ***, p

    Journal: The Journal of Biological Chemistry

    Article Title: Pancreatic β-Cell Death due to Pdx-1 Deficiency Requires Multi-BH Domain Protein Bax but Not Bak *

    doi: 10.1074/jbc.M115.705293

    Figure Lengend Snippet: Pdx1 knockdown induces Bax activation in MIN6 cells and islets. A , Bax and Bak mRNA levels in control and Pdx1 KD cells. 3 days after Pdx1 KD in MIN6 cells, Bax and Bak mRNA levels were not different between control and Pdx1 KD cells ( n = 3). B , Western blot of Pdx1 KD cells. 3 days after Pdx1 KD in MIN6 cells, immunoblot analysis was performed to determine Pdx1, Bax, and Bak in Pdx1 KD MIN6 cells. C , Bax nuclear/cytosolic translocation in Pdx1 KD MIN6 cells. 3 days after Pdx1 shRNA lentivirus infection, cytosolic and nuclear proteins were analyzed by Western blot. D , immunoprecipitate of Bax. 3 days after Pdx1 KD in MIN6 cells, cells were lysed in 1% CHAPS and then immunoprecipitated with the 6A7 anti-BAX antibody. Immunoprecipitates were analyzed by anti-BAX (N20) immunoblots. E , effects of Pdx1 on ΔΨ m . MIn6 cells were treated with Pdx1 shRNA lentivirus for 0, 4 days. Cells were stained with TMRE dye to measure ΔΨ m . ***, p

    Article Snippet: The blots were probed with antibodies against Pdx1 (07-696; Millipore), cytochrome c (mouse6H2.B4, Millipore), Actin (A-2066; Sigma), Bax (6A7) (2281-MC-100; Trevigen), Bax (N20) (SC493, Santa Cruz Biotechnology) and Bak (06536, Millipore).

    Techniques: Activation Assay, Western Blot, Translocation Assay, shRNA, Infection, Immunoprecipitation, Staining

    Bax gene ablation in islets inhibits diabetes in Pdx1 +/− Bax F/F Bak −/− Cre − mice. A and B , blood glucose levels after intraperitoneal injection of dextrose (1 g/kg) in the male mice on a HFD for 13 weeks. **, p

    Journal: The Journal of Biological Chemistry

    Article Title: Pancreatic β-Cell Death due to Pdx-1 Deficiency Requires Multi-BH Domain Protein Bax but Not Bak *

    doi: 10.1074/jbc.M115.705293

    Figure Lengend Snippet: Bax gene ablation in islets inhibits diabetes in Pdx1 +/− Bax F/F Bak −/− Cre − mice. A and B , blood glucose levels after intraperitoneal injection of dextrose (1 g/kg) in the male mice on a HFD for 13 weeks. **, p

    Article Snippet: The blots were probed with antibodies against Pdx1 (07-696; Millipore), cytochrome c (mouse6H2.B4, Millipore), Actin (A-2066; Sigma), Bax (6A7) (2281-MC-100; Trevigen), Bax (N20) (SC493, Santa Cruz Biotechnology) and Bak (06536, Millipore).

    Techniques: Mouse Assay, Injection

    Bax ablation protects β-cells in adult Pdx1 +/− Bax F/F Bak −/− Cre − mice. A and B , Western blot of Bax in islets from 4-month old mice with normal chow ( A ) and high fat diet ( B ). C , islet morphology in adult mouse after 12 weeks on a high fat diet. Anti-insulin and anti-glucagon antibodies were used to stain β-cells ( red ) and α cells ( green ) respectively. Scale bar, 20 μm. D , histological analysis of pancreatic islets and quantitation of group data for β-cell mass ( n = 3–5 per group). **, p

    Journal: The Journal of Biological Chemistry

    Article Title: Pancreatic β-Cell Death due to Pdx-1 Deficiency Requires Multi-BH Domain Protein Bax but Not Bak *

    doi: 10.1074/jbc.M115.705293

    Figure Lengend Snippet: Bax ablation protects β-cells in adult Pdx1 +/− Bax F/F Bak −/− Cre − mice. A and B , Western blot of Bax in islets from 4-month old mice with normal chow ( A ) and high fat diet ( B ). C , islet morphology in adult mouse after 12 weeks on a high fat diet. Anti-insulin and anti-glucagon antibodies were used to stain β-cells ( red ) and α cells ( green ) respectively. Scale bar, 20 μm. D , histological analysis of pancreatic islets and quantitation of group data for β-cell mass ( n = 3–5 per group). **, p

    Article Snippet: The blots were probed with antibodies against Pdx1 (07-696; Millipore), cytochrome c (mouse6H2.B4, Millipore), Actin (A-2066; Sigma), Bax (6A7) (2281-MC-100; Trevigen), Bax (N20) (SC493, Santa Cruz Biotechnology) and Bak (06536, Millipore).

    Techniques: Mouse Assay, Western Blot, Staining, Quantitation Assay