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ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) <t>RT-qPCR</t> analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.
Chamq Universal Sybr Qpcr Mastermix Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) <t>RT-qPCR</t> analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.
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ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) <t>RT-qPCR</t> analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.
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ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) <t>RT-qPCR</t> analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.
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ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) <t>RT-qPCR</t> analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.
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ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) <t>RT-qPCR</t> analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.
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ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) <t>RT-qPCR</t> analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.
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ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) <t>RT-qPCR</t> analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.
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evagreen 2 × qpcr mastermix  (Applied Biological Materials Inc)
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ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) <t>RT-qPCR</t> analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.
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ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) RT-qPCR analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.

Journal: Synthetic and Systems Biotechnology

Article Title: XRE-type transcriptional regulator ProR controls prodigiosin synthesis in Serratia marcescens JNB5-1

doi: 10.1016/j.synbio.2026.02.014

Figure Lengend Snippet: ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) RT-qPCR analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.

Article Snippet: RT-qPCR analyses were performed on 200 ng/μL cDNA with ChamQ Universal SYBR qPCR mastermix kit (Vazyme) and primers from .

Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Electrophoretic Mobility Shift Assay, Binding Assay