Journal: Nanoscale Advances

Article Title: Simultaneous activation and release of a microtubule inhibitor from amphiphilic polymeric nanoparticles

doi: 10.1039/d6na00763e

Figure Lengend Snippet: Representative lowest energy docking poses of (a) cis -PST-1, (b) trans -PST-1, (c) cis -proPST-1, and (d) trans -proPST-1 within the colchicine binding pocket of tubulin (PDB ID: 5LYJ). Ligands are represented as orange sticks, while interacting amino acid residues are shown as yellow sticks and labelled using single-letter amino acid notation. Hydrogen-bonding and hydrophobic interactions are indicated by red and blue dashed lines, respectively, with corresponding interaction distances (Å) shown. Structures and interaction maps were generated using PyMOL v3.0.

Article Snippet: Tubulin MAP-rich (porcine brain) (Cat. #ML116), Tubulin protein (fluorescent HiLyte 488): porcine brain (Cat. #TL488M), General tubulin buffer (Cat. #BST01), Tubulin glycerol buffer (Cat. #BST05) and Taxol (Cat. #TXD01) and GTP (Cat. #BTS06) were obtained from Cytoskeleton, Inc and used as per the instruction manual.

Techniques: Docking Assay, Binding Assay, Generated

Journal: Nanoscale Advances

Article Title: Simultaneous activation and release of a microtubule inhibitor from amphiphilic polymeric nanoparticles

doi: 10.1039/d6na00763e

Figure Lengend Snippet: (a) Schematic representation of light-controlled inhibition of microtubule polymerisation by proPST-1-loaded polymer nanoparticles (proPST-1@PN). Under dark conditions, the encapsulated photocaged prodrug remains inactive, allowing normal microtubule formation. Upon 365 nm irradiation, photolytic uncaging and release of active cis -PST-1 suppress microtubule assembly. Created in BioRender. Singh, H. (2026) https://BioRender.com/fac5r0eT . (b) Tubulin polymerisation kinetics monitored by absorbance at 340 nm for control samples, empty polymer nanoparticles (PN control), and proPST-1@PN under dark and irradiated conditions. Irradiation of proPST-1@PN results in pronounced inhibition of tubulin polymerisation, whereas no inhibition is observed in the dark. (c) Fluorescence microscopy images of microtubule structures formed in the presence of control samples, proPST-1@PN under dark conditions, and irradiated proPST-1@PN. Normal microtubule growth and network formation are observed in control and dark conditions, while irradiation results in substantial disruption of microtubule assembly. Scale bars: 10 µm.

Article Snippet: Tubulin MAP-rich (porcine brain) (Cat. #ML116), Tubulin protein (fluorescent HiLyte 488): porcine brain (Cat. #TL488M), General tubulin buffer (Cat. #BST01), Tubulin glycerol buffer (Cat. #BST05) and Taxol (Cat. #TXD01) and GTP (Cat. #BTS06) were obtained from Cytoskeleton, Inc and used as per the instruction manual.

Techniques: Inhibition, Polymer, Irradiation, Control, Fluorescence, Microscopy, Disruption