manual microarray platform  (Thermo Fisher)


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    Structured Review

    Thermo Fisher manual microarray platform
    Manual Microarray Platform, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/manual microarray platform/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    manual microarray platform - by Bioz Stars, 2020-09
    90/100 stars

    Related Products / Commonly Used Together

    in‐vitro blood tests
    microtest system
    singleplex test system

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    Related Articles

    Microarray:

    Article Title: Evaluation of a novel automated allergy microarray platform compared with three other allergy test methods
    Article Snippet: .. We thus chose to pursue the practical alternative strategy of comparing results generated by the Microtest system with results from three other established allergy test methods: SPT and two in‐vitro blood tests, one singleplex test system (ImmunoCAP, manufactured by Phadia AB, Uppsala, Sweden) and one manual microarray platform [Immuno‐Solid phase Allergen Chip (ISAC) 112, manufactured by Phadia AB]. .. Regardless of which diagnostic method is used, it is important to use the clinical history to interpret the test results because a positive skin or blood test yields information on IgE sensitization, which is not always equivalent to clinical allergy.

    Generated:

    Article Title: Evaluation of a novel automated allergy microarray platform compared with three other allergy test methods
    Article Snippet: .. We thus chose to pursue the practical alternative strategy of comparing results generated by the Microtest system with results from three other established allergy test methods: SPT and two in‐vitro blood tests, one singleplex test system (ImmunoCAP, manufactured by Phadia AB, Uppsala, Sweden) and one manual microarray platform [Immuno‐Solid phase Allergen Chip (ISAC) 112, manufactured by Phadia AB]. .. Regardless of which diagnostic method is used, it is important to use the clinical history to interpret the test results because a positive skin or blood test yields information on IgE sensitization, which is not always equivalent to clinical allergy.

    Chromatin Immunoprecipitation:

    Article Title: Evaluation of a novel automated allergy microarray platform compared with three other allergy test methods
    Article Snippet: .. We thus chose to pursue the practical alternative strategy of comparing results generated by the Microtest system with results from three other established allergy test methods: SPT and two in‐vitro blood tests, one singleplex test system (ImmunoCAP, manufactured by Phadia AB, Uppsala, Sweden) and one manual microarray platform [Immuno‐Solid phase Allergen Chip (ISAC) 112, manufactured by Phadia AB]. .. Regardless of which diagnostic method is used, it is important to use the clinical history to interpret the test results because a positive skin or blood test yields information on IgE sensitization, which is not always equivalent to clinical allergy.

    In Vitro:

    Article Title: Evaluation of a novel automated allergy microarray platform compared with three other allergy test methods
    Article Snippet: .. We thus chose to pursue the practical alternative strategy of comparing results generated by the Microtest system with results from three other established allergy test methods: SPT and two in‐vitro blood tests, one singleplex test system (ImmunoCAP, manufactured by Phadia AB, Uppsala, Sweden) and one manual microarray platform [Immuno‐Solid phase Allergen Chip (ISAC) 112, manufactured by Phadia AB]. .. Regardless of which diagnostic method is used, it is important to use the clinical history to interpret the test results because a positive skin or blood test yields information on IgE sensitization, which is not always equivalent to clinical allergy.

    Single-particle Tracking:

    Article Title: Evaluation of a novel automated allergy microarray platform compared with three other allergy test methods
    Article Snippet: .. We thus chose to pursue the practical alternative strategy of comparing results generated by the Microtest system with results from three other established allergy test methods: SPT and two in‐vitro blood tests, one singleplex test system (ImmunoCAP, manufactured by Phadia AB, Uppsala, Sweden) and one manual microarray platform [Immuno‐Solid phase Allergen Chip (ISAC) 112, manufactured by Phadia AB]. .. Regardless of which diagnostic method is used, it is important to use the clinical history to interpret the test results because a positive skin or blood test yields information on IgE sensitization, which is not always equivalent to clinical allergy.

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    Thermo Fisher sgc 7901 cells
    miR-593-5p suppressed gastric cancer (GC) cell invasion. Notes: ( A and B ) Images of cell cycles after transfection with miR-593-5p lentivirus was assessed by transwell assay in <t>SGC-7901</t> and MGC-803 cells (×200). ( C and D ) Data represent mean ± SD of three duplication number of invasive cells in each group. * P
    Sgc 7901 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sgc 7901 cells/product/Thermo Fisher
    Average 94 stars, based on 85 article reviews
    Price from $9.99 to $1999.99
    sgc 7901 cells - by Bioz Stars, 2020-09
    94/100 stars
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    miR-593-5p suppressed gastric cancer (GC) cell invasion. Notes: ( A and B ) Images of cell cycles after transfection with miR-593-5p lentivirus was assessed by transwell assay in SGC-7901 and MGC-803 cells (×200). ( C and D ) Data represent mean ± SD of three duplication number of invasive cells in each group. * P

    Journal: OncoTargets and therapy

    Article Title: Regulation of cell proliferation and metastasis by microRNA-593-5p in human gastric cancer

    doi: 10.2147/OTT.S178151

    Figure Lengend Snippet: miR-593-5p suppressed gastric cancer (GC) cell invasion. Notes: ( A and B ) Images of cell cycles after transfection with miR-593-5p lentivirus was assessed by transwell assay in SGC-7901 and MGC-803 cells (×200). ( C and D ) Data represent mean ± SD of three duplication number of invasive cells in each group. * P

    Article Snippet: Transfection of MGC-803 and SGC-7901 cells was carried out by Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Techniques: Transfection, Transwell Assay

    miR-593-5p suppressed gastric cancer (GC) cell migration. Notes: ( A and C ) Images of cell migration after transfection with miR-593-5p lentivirus was assessed by wound-healing assay in SGC-7901 and MGC-803 cells. ( B and D ) Data represent mean ± SD of four duplication migration rate in 48 and 72 hours (h) in each group. * P

    Journal: OncoTargets and therapy

    Article Title: Regulation of cell proliferation and metastasis by microRNA-593-5p in human gastric cancer

    doi: 10.2147/OTT.S178151

    Figure Lengend Snippet: miR-593-5p suppressed gastric cancer (GC) cell migration. Notes: ( A and C ) Images of cell migration after transfection with miR-593-5p lentivirus was assessed by wound-healing assay in SGC-7901 and MGC-803 cells. ( B and D ) Data represent mean ± SD of four duplication migration rate in 48 and 72 hours (h) in each group. * P

    Article Snippet: Transfection of MGC-803 and SGC-7901 cells was carried out by Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Techniques: Migration, Transfection, Wound Healing Assay

    miR-593-5p suppressed gastric cancer (GC) cell growth. Notes: ( A and B ) miR-593-5p transfection efficiency after transfection with miR-593-5p lentivirus was assessed by quantitative RT-PCR (qRT-PCR) in SGC-7901 and MGC-803 cells. ( C and D ) Proliferation of GC cells after transfection with miR-593-5p lentivirus was assessed by CCK-8 assay in SGC-7901 and MGC-803 cells. * P

    Journal: OncoTargets and therapy

    Article Title: Regulation of cell proliferation and metastasis by microRNA-593-5p in human gastric cancer

    doi: 10.2147/OTT.S178151

    Figure Lengend Snippet: miR-593-5p suppressed gastric cancer (GC) cell growth. Notes: ( A and B ) miR-593-5p transfection efficiency after transfection with miR-593-5p lentivirus was assessed by quantitative RT-PCR (qRT-PCR) in SGC-7901 and MGC-803 cells. ( C and D ) Proliferation of GC cells after transfection with miR-593-5p lentivirus was assessed by CCK-8 assay in SGC-7901 and MGC-803 cells. * P

    Article Snippet: Transfection of MGC-803 and SGC-7901 cells was carried out by Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Techniques: Transfection, Quantitative RT-PCR, CCK-8 Assay

    miR-593-5p suppressed gastric cancer (GC) growth and metastasis in vivo. Notes: ( A ) Images of tumors isolated from nude mice that were injected for each cell line group after 36 days. The tumor, lung, and liver tissues were assessed by H E stained in each group (×100). ( B and C ) Growth curves of tumor resulting from injection of SGC-7901 or MGC-803 cells into nude mice. The tumor volumes were estimated using calipers. ( D and E ) The last time volume of tumor isolated from nude mice. Data represent mean ± SD of 12 mice per group. * P

    Journal: OncoTargets and therapy

    Article Title: Regulation of cell proliferation and metastasis by microRNA-593-5p in human gastric cancer

    doi: 10.2147/OTT.S178151

    Figure Lengend Snippet: miR-593-5p suppressed gastric cancer (GC) growth and metastasis in vivo. Notes: ( A ) Images of tumors isolated from nude mice that were injected for each cell line group after 36 days. The tumor, lung, and liver tissues were assessed by H E stained in each group (×100). ( B and C ) Growth curves of tumor resulting from injection of SGC-7901 or MGC-803 cells into nude mice. The tumor volumes were estimated using calipers. ( D and E ) The last time volume of tumor isolated from nude mice. Data represent mean ± SD of 12 mice per group. * P

    Article Snippet: Transfection of MGC-803 and SGC-7901 cells was carried out by Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Techniques: In Vivo, Isolation, Mouse Assay, Injection, Staining

    miR-593-5p suppressed G1/S transition. Notes: ( A and B ) Images of cell cycles after transfection with miR-593-5p lentivirus was assessed by flow cytometric method in SGC-7901 and MGC-803 cells. ( C and D ) Data represent mean ± SD of three duplicative experiment respective phase of cell cycle rate in each group. * P

    Journal: OncoTargets and therapy

    Article Title: Regulation of cell proliferation and metastasis by microRNA-593-5p in human gastric cancer

    doi: 10.2147/OTT.S178151

    Figure Lengend Snippet: miR-593-5p suppressed G1/S transition. Notes: ( A and B ) Images of cell cycles after transfection with miR-593-5p lentivirus was assessed by flow cytometric method in SGC-7901 and MGC-803 cells. ( C and D ) Data represent mean ± SD of three duplicative experiment respective phase of cell cycle rate in each group. * P

    Article Snippet: Transfection of MGC-803 and SGC-7901 cells was carried out by Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Techniques: Transfection, Flow Cytometry, Cytometry

    miR-593-5p is lowly expressed in gastric cancer (GC) tissues and cell lines. Notes: ( A ) miR-593-5p expression was assessed by quantitative RT-PCR (qRT-PCR) in 73 GC tumor specimens as compared to normal adjacent gastric tissue. ( B ) miR-593-5p expression was assessed by qRT-PCR in GC cells (HGC-27, AGS, SGC-7901, MGC-803) and normal gastric epithelial cells (GES). U6 was assessed as an internal control. * P

    Journal: OncoTargets and therapy

    Article Title: Regulation of cell proliferation and metastasis by microRNA-593-5p in human gastric cancer

    doi: 10.2147/OTT.S178151

    Figure Lengend Snippet: miR-593-5p is lowly expressed in gastric cancer (GC) tissues and cell lines. Notes: ( A ) miR-593-5p expression was assessed by quantitative RT-PCR (qRT-PCR) in 73 GC tumor specimens as compared to normal adjacent gastric tissue. ( B ) miR-593-5p expression was assessed by qRT-PCR in GC cells (HGC-27, AGS, SGC-7901, MGC-803) and normal gastric epithelial cells (GES). U6 was assessed as an internal control. * P

    Article Snippet: Transfection of MGC-803 and SGC-7901 cells was carried out by Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Techniques: Expressing, Quantitative RT-PCR

    miR-593-5p negatively regulates MST4 and relative signal pathway. Notes: ( A ) Potential target genes of miR-593-5p were screened by microarray gene expression profiling combined with bioinformatics target prediction. ( B and C ) Data represent mean ± SD of three duplication mRNA expression of potential target genes by quantitative RT-PCR (qRT-PCR) in each group; GAPDH was assessed as an internal control. ( D ) Images of genes’ protein expression after transfection with miR-593-5p lentivirus was assessed by Western blot assay in SGC-7901 and MGC-803 cells. ( E and F ) Data represent mean ± SD of relative protein expression of genes in each group. GAPDH was assessed as an internal control. ( G ) The predicted interaction site of miR-593-5p and candidate target gene MST4 wild-type 3′-untranslated region (3′-UTR) and serial deleted forms of the 3′-UTR reporters. Data represent mean ± SD (n=3) of relative luciferase activity of each group by luciferase assay co-transfected with miR-593-5p and IRREPORT-MST4 plasmid (miR-NC and miR-593-5p with MST4 WT 3′-UTR, miR-NC, and miR-593-5p with MST4 MUT 3′-UTR) after 24 hours. * P > 0.05 compared to controls. Abbreviations: Ctrl, control; NC, negative control.

    Journal: OncoTargets and therapy

    Article Title: Regulation of cell proliferation and metastasis by microRNA-593-5p in human gastric cancer

    doi: 10.2147/OTT.S178151

    Figure Lengend Snippet: miR-593-5p negatively regulates MST4 and relative signal pathway. Notes: ( A ) Potential target genes of miR-593-5p were screened by microarray gene expression profiling combined with bioinformatics target prediction. ( B and C ) Data represent mean ± SD of three duplication mRNA expression of potential target genes by quantitative RT-PCR (qRT-PCR) in each group; GAPDH was assessed as an internal control. ( D ) Images of genes’ protein expression after transfection with miR-593-5p lentivirus was assessed by Western blot assay in SGC-7901 and MGC-803 cells. ( E and F ) Data represent mean ± SD of relative protein expression of genes in each group. GAPDH was assessed as an internal control. ( G ) The predicted interaction site of miR-593-5p and candidate target gene MST4 wild-type 3′-untranslated region (3′-UTR) and serial deleted forms of the 3′-UTR reporters. Data represent mean ± SD (n=3) of relative luciferase activity of each group by luciferase assay co-transfected with miR-593-5p and IRREPORT-MST4 plasmid (miR-NC and miR-593-5p with MST4 WT 3′-UTR, miR-NC, and miR-593-5p with MST4 MUT 3′-UTR) after 24 hours. * P > 0.05 compared to controls. Abbreviations: Ctrl, control; NC, negative control.

    Article Snippet: Transfection of MGC-803 and SGC-7901 cells was carried out by Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Techniques: Microarray, Expressing, Quantitative RT-PCR, Transfection, Western Blot, Luciferase, Activity Assay, Plasmid Preparation, Negative Control