mammary epithelial cell basal medium  (ATCC)


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    ATCC mammary epithelial cell basal medium
    Mammary Epithelial Cell Basal Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mammary epithelial cell basal medium/product/ATCC
    Average 97 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    mammary epithelial cell basal medium - by Bioz Stars, 2022-12
    97/100 stars

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  • mebm  (ATCC)
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    ATCC mebm
    Vitexin ameliorated <t>cell</t> damage on S. <t>aureus</t> <t>challenged-MAC-T</t> cells. (a) MAC-T cells were stimulated with S. aureus at MOI of 100 for 6 h. And then, different concentrations of vitexin (10, 20, and 40 μ M) were added to coincubate for 24 h. Flow cytometry was used to evaluate the effect of vitexin on the apoptosis of MAC-T cells stimulated by S. aureus . The horizontal axis represented Annexin V-FITC, and the vertical axis expressed PI. (b) Flow cytometry results were statistically analyzed for apoptosis rate by the GraphPad Prism 9.00 software. (c–e) The cells were dealt with S. aureus and/or vitexin; the relative expression of TNF- α , IL-1 β , and IL-6 mRNA was examined by RT-qPCR. GAPDH served as an internal reference gene. (f–h) The proinflammatory factors produced were tested by ELISA. Data were presented as means ± SEM of three independent experiments. # p
    Mebm, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mebm/product/ATCC
    Average 96 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    mebm - by Bioz Stars, 2022-12
    96/100 stars
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    Vitexin ameliorated cell damage on S. aureus challenged-MAC-T cells. (a) MAC-T cells were stimulated with S. aureus at MOI of 100 for 6 h. And then, different concentrations of vitexin (10, 20, and 40 μ M) were added to coincubate for 24 h. Flow cytometry was used to evaluate the effect of vitexin on the apoptosis of MAC-T cells stimulated by S. aureus . The horizontal axis represented Annexin V-FITC, and the vertical axis expressed PI. (b) Flow cytometry results were statistically analyzed for apoptosis rate by the GraphPad Prism 9.00 software. (c–e) The cells were dealt with S. aureus and/or vitexin; the relative expression of TNF- α , IL-1 β , and IL-6 mRNA was examined by RT-qPCR. GAPDH served as an internal reference gene. (f–h) The proinflammatory factors produced were tested by ELISA. Data were presented as means ± SEM of three independent experiments. # p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Vitexin Mitigates Staphylococcus aureus-Induced Mastitis via Regulation of ROS/ER Stress/NF-κB/MAPK Pathway

    doi: 10.1155/2022/7977433

    Figure Lengend Snippet: Vitexin ameliorated cell damage on S. aureus challenged-MAC-T cells. (a) MAC-T cells were stimulated with S. aureus at MOI of 100 for 6 h. And then, different concentrations of vitexin (10, 20, and 40 μ M) were added to coincubate for 24 h. Flow cytometry was used to evaluate the effect of vitexin on the apoptosis of MAC-T cells stimulated by S. aureus . The horizontal axis represented Annexin V-FITC, and the vertical axis expressed PI. (b) Flow cytometry results were statistically analyzed for apoptosis rate by the GraphPad Prism 9.00 software. (c–e) The cells were dealt with S. aureus and/or vitexin; the relative expression of TNF- α , IL-1 β , and IL-6 mRNA was examined by RT-qPCR. GAPDH served as an internal reference gene. (f–h) The proinflammatory factors produced were tested by ELISA. Data were presented as means ± SEM of three independent experiments. # p

    Article Snippet: The cow mammary epithelial cell line MAC-T was purchased from the American Type Culture Collection (ATCC, USA).

    Techniques: Flow Cytometry, Software, Expressing, Quantitative RT-PCR, Produced, Enzyme-linked Immunosorbent Assay

    Vitexin inhibited ROS production on S. aureus stimulated-MAC-T cells. (a) The cells were stimulated with S. aureus for 6 h and then treated with different concentrations of vitexin (10, 20, and 40 μ M) for 24 h. ROS red fluorescence was displayed with DHE probe. (b) ROS levels were quantified by the ImageJ software. Relative ROS levels were expressed as red fluorescence intensity/cell number. (c) The level of T-AOC. (d) The activity of SOD. (e) The activity of GSH-PX. (f) The activity of CAT. (g) The concentration of MDA. (h) Western blot detection of the expression level of PPAR γ . (i) PPAR γ gray values were measured by the ImageJ software. (j) MAC-T cells were pretreated with GW9662 (10 μ M) or GW1929 (10 μ M) for 30 min to downregulate or upregulate the expression of PPAR γ , respectively, then stimulated with S. aureus to mimic inflammatory stimulation, and finally treated with vitexin. ROS was detected with DHE probe. Data was expressed as means ± SEM of three independent experiments. # p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Vitexin Mitigates Staphylococcus aureus-Induced Mastitis via Regulation of ROS/ER Stress/NF-κB/MAPK Pathway

    doi: 10.1155/2022/7977433

    Figure Lengend Snippet: Vitexin inhibited ROS production on S. aureus stimulated-MAC-T cells. (a) The cells were stimulated with S. aureus for 6 h and then treated with different concentrations of vitexin (10, 20, and 40 μ M) for 24 h. ROS red fluorescence was displayed with DHE probe. (b) ROS levels were quantified by the ImageJ software. Relative ROS levels were expressed as red fluorescence intensity/cell number. (c) The level of T-AOC. (d) The activity of SOD. (e) The activity of GSH-PX. (f) The activity of CAT. (g) The concentration of MDA. (h) Western blot detection of the expression level of PPAR γ . (i) PPAR γ gray values were measured by the ImageJ software. (j) MAC-T cells were pretreated with GW9662 (10 μ M) or GW1929 (10 μ M) for 30 min to downregulate or upregulate the expression of PPAR γ , respectively, then stimulated with S. aureus to mimic inflammatory stimulation, and finally treated with vitexin. ROS was detected with DHE probe. Data was expressed as means ± SEM of three independent experiments. # p

    Article Snippet: The cow mammary epithelial cell line MAC-T was purchased from the American Type Culture Collection (ATCC, USA).

    Techniques: Fluorescence, Software, Activity Assay, Concentration Assay, Multiple Displacement Amplification, Western Blot, Expressing

    The purified substance vitexin had no effect on the viability of MAC-T cells. (a) The chemical structure of vitexin. (b) HPLC for purity inspection of vitexin. (c) CCK-8 assessed the effect of vitexin on the viability of MAC-T cells.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Vitexin Mitigates Staphylococcus aureus-Induced Mastitis via Regulation of ROS/ER Stress/NF-κB/MAPK Pathway

    doi: 10.1155/2022/7977433

    Figure Lengend Snippet: The purified substance vitexin had no effect on the viability of MAC-T cells. (a) The chemical structure of vitexin. (b) HPLC for purity inspection of vitexin. (c) CCK-8 assessed the effect of vitexin on the viability of MAC-T cells.

    Article Snippet: The cow mammary epithelial cell line MAC-T was purchased from the American Type Culture Collection (ATCC, USA).

    Techniques: Purification, High Performance Liquid Chromatography, CCK-8 Assay

    Vitexin suppressed the activation of MAPK and NF- κ B signaling pathways by blocking the ER stress caused by S. aureus on MAC-T cells. (a) The protein levels of PDI, BiP, Ero1-L α , p-IRE1 α , PERK, p-eIF2 α , and CHOP were detected by Western blot on MAC-T cells. (c) The expression levels of JNK, ERK, p38, and p65 proteins in MAC-T cells were determined by Western blot. (b, d) Protein gray values were measured by the ImageJ software. Data was expressed as means ± SEM of three independent experiments. # p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Vitexin Mitigates Staphylococcus aureus-Induced Mastitis via Regulation of ROS/ER Stress/NF-κB/MAPK Pathway

    doi: 10.1155/2022/7977433

    Figure Lengend Snippet: Vitexin suppressed the activation of MAPK and NF- κ B signaling pathways by blocking the ER stress caused by S. aureus on MAC-T cells. (a) The protein levels of PDI, BiP, Ero1-L α , p-IRE1 α , PERK, p-eIF2 α , and CHOP were detected by Western blot on MAC-T cells. (c) The expression levels of JNK, ERK, p38, and p65 proteins in MAC-T cells were determined by Western blot. (b, d) Protein gray values were measured by the ImageJ software. Data was expressed as means ± SEM of three independent experiments. # p

    Article Snippet: The cow mammary epithelial cell line MAC-T was purchased from the American Type Culture Collection (ATCC, USA).

    Techniques: Activation Assay, Blocking Assay, Western Blot, Expressing, Software