malt extract broth  (Merck KGaA)

 
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    Merck KGaA malt extract broth
    Malt Extract Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    malt extract broth  (Merck KGaA)

     
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    Merck KGaA malt extract broth
    Malt Extract Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    malt extract broth meb  (Merck KGaA)

     
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    Merck KGaA malt extract broth meb
    Malt Extract Broth Meb, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/malt extract broth meb/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
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    malt extract broth  (Merck KGaA)

     
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    Merck KGaA malt extract broth
    Malt Extract Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/malt extract broth/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    malt extract broth - by Bioz Stars, 2023-11
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    malt extract broth  (Merck KGaA)

     
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    Merck KGaA malt extract broth
    Malt Extract Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/malt extract broth/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    malt extract broth - by Bioz Stars, 2023-11
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    Merck KGaA malt extract broth
    Malt Extract Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/malt extract broth/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
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    Merck KGaA malt extract broth
    Malt Extract Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/malt extract broth/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    malt extract broth - by Bioz Stars, 2023-11
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    malt extract broth medium meb  (Merck KGaA)

     
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    Merck KGaA malt extract broth medium meb
    Malt Extract Broth Medium Meb, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/malt extract broth medium meb/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    malt extract broth meb  (Merck KGaA)

     
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    Merck KGaA malt extract broth meb
    Malt Extract Broth Meb, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/malt extract broth meb/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
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    malt extract broth meb  (Merck KGaA)

     
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    Merck KGaA malt extract broth meb
    Growth rate and morphology of Hortaea werneckii during experimental evolution at high salinity. ( A ) H. werneckii progenitor (T0) and evolved strains (T50-1, T100-1; T50-2, T100-2); pre-cultures were diluted to OD 600 0.5 and two 10-fold serial dilutions were spotted onto MEA plates without osmolyte or <t>with</t> <t>NaCl,</t> KCl, sorbitol, or glycerol, as indicated. ( B ) Growth of H. werneckii progenitor and evolved strains in <t>MEB</t> without or supplemented with 4.3 M NaCl, as measured by OD 600 over 160 h of incubation. Data are means ± standard deviation (SD) of triplicate experiments. Direct cell counts (number of cells per millilitre) of H. werneckii progenitor and evolved strains at OD 600 0.5 and 1.0 are listed within the rectangles in the graph (in the same order as in the graph legend). ( C , D ) Cell chain length, number of septa in individual cell chains and cell width of H. werneckii progenitor (T0) and selected evolved cultures (T50-1, T100-1) measured on 120 cell chains after growth to OD 600 0.5 in MEB without NaCl (panel C) or with 4.3 M NaCl (panel D), where significantly different ( p ≤ 0.001) pairs of data are marked with blue horizontal lines. Outliers are marked with black dots. Measurements were made in two independent experiments that yielded similar data, with one representative experiment shown. ( D ) H. werneckii progenitor and evolved strains were grown in MEB with to OD 600 0.5. Microscopy images were then taken and processed as in panel C. Representative images of DAPI-stained H. werneckii cells of T0, T50-1, and T100-1 in MEB containing 4.3 M NaCl are shown in panel D. Black arrows mark multicellular chains with multiple septa. The length of the scale bars is 20 µm.
    Malt Extract Broth Meb, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/malt extract broth meb/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    malt extract broth meb - by Bioz Stars, 2023-11
    86/100 stars

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    1) Product Images from "Seven Years at High Salinity—Experimental Evolution of the Extremely Halotolerant Black Yeast Hortaea werneckii"

    Article Title: Seven Years at High Salinity—Experimental Evolution of the Extremely Halotolerant Black Yeast Hortaea werneckii

    Journal: Journal of Fungi

    doi: 10.3390/jof7090723

    Growth rate and morphology of Hortaea werneckii during experimental evolution at high salinity. ( A ) H. werneckii progenitor (T0) and evolved strains (T50-1, T100-1; T50-2, T100-2); pre-cultures were diluted to OD 600 0.5 and two 10-fold serial dilutions were spotted onto MEA plates without osmolyte or with NaCl, KCl, sorbitol, or glycerol, as indicated. ( B ) Growth of H. werneckii progenitor and evolved strains in MEB without or supplemented with 4.3 M NaCl, as measured by OD 600 over 160 h of incubation. Data are means ± standard deviation (SD) of triplicate experiments. Direct cell counts (number of cells per millilitre) of H. werneckii progenitor and evolved strains at OD 600 0.5 and 1.0 are listed within the rectangles in the graph (in the same order as in the graph legend). ( C , D ) Cell chain length, number of septa in individual cell chains and cell width of H. werneckii progenitor (T0) and selected evolved cultures (T50-1, T100-1) measured on 120 cell chains after growth to OD 600 0.5 in MEB without NaCl (panel C) or with 4.3 M NaCl (panel D), where significantly different ( p ≤ 0.001) pairs of data are marked with blue horizontal lines. Outliers are marked with black dots. Measurements were made in two independent experiments that yielded similar data, with one representative experiment shown. ( D ) H. werneckii progenitor and evolved strains were grown in MEB with to OD 600 0.5. Microscopy images were then taken and processed as in panel C. Representative images of DAPI-stained H. werneckii cells of T0, T50-1, and T100-1 in MEB containing 4.3 M NaCl are shown in panel D. Black arrows mark multicellular chains with multiple septa. The length of the scale bars is 20 µm.
    Figure Legend Snippet: Growth rate and morphology of Hortaea werneckii during experimental evolution at high salinity. ( A ) H. werneckii progenitor (T0) and evolved strains (T50-1, T100-1; T50-2, T100-2); pre-cultures were diluted to OD 600 0.5 and two 10-fold serial dilutions were spotted onto MEA plates without osmolyte or with NaCl, KCl, sorbitol, or glycerol, as indicated. ( B ) Growth of H. werneckii progenitor and evolved strains in MEB without or supplemented with 4.3 M NaCl, as measured by OD 600 over 160 h of incubation. Data are means ± standard deviation (SD) of triplicate experiments. Direct cell counts (number of cells per millilitre) of H. werneckii progenitor and evolved strains at OD 600 0.5 and 1.0 are listed within the rectangles in the graph (in the same order as in the graph legend). ( C , D ) Cell chain length, number of septa in individual cell chains and cell width of H. werneckii progenitor (T0) and selected evolved cultures (T50-1, T100-1) measured on 120 cell chains after growth to OD 600 0.5 in MEB without NaCl (panel C) or with 4.3 M NaCl (panel D), where significantly different ( p ≤ 0.001) pairs of data are marked with blue horizontal lines. Outliers are marked with black dots. Measurements were made in two independent experiments that yielded similar data, with one representative experiment shown. ( D ) H. werneckii progenitor and evolved strains were grown in MEB with to OD 600 0.5. Microscopy images were then taken and processed as in panel C. Representative images of DAPI-stained H. werneckii cells of T0, T50-1, and T100-1 in MEB containing 4.3 M NaCl are shown in panel D. Black arrows mark multicellular chains with multiple septa. The length of the scale bars is 20 µm.

    Techniques Used: Incubation, Standard Deviation, Microscopy, Staining

    Morphology of Hortaea werneckii exposed to caspofungin and various osmolytes. ( A ) Progenitor (T0) Hortaea werneckii and evolved strains (T50-1, T100-1; T50-2, T100-2) were grown in MEB without osmolyte or with 1.8 M, 3 M, or 4.3 M NaCl in the presence of 50 ng/mL of the cell wall synthesis inhibitor caspofungin. Microscopy images were taken when the cultures reached an OD 600 of 0.5. Two independent experiments yielded similar data, and the representative images of one experiment are shown. The length of the scale bars is 20 µm. Inlays show representative examples of casponfungin-caused changed morphology. ( B ) Melanisation of H. werneckii progenitor and evolved strains grown in the presence of different solutes. Pre-cultures diluted to OD 600 0.5 were spotted at 10-fold serial dilutions on MEA plates without osmolyte or with sorbitol, glycerol, NaCl, or KCl as indicated. Images were taken after 9 days incubation, except for 1 M sorbitol (taken after 6 days) and 4.3 M NaCl (taken after 14 days). The experiment was performed in triplicate and the representative images are shown.
    Figure Legend Snippet: Morphology of Hortaea werneckii exposed to caspofungin and various osmolytes. ( A ) Progenitor (T0) Hortaea werneckii and evolved strains (T50-1, T100-1; T50-2, T100-2) were grown in MEB without osmolyte or with 1.8 M, 3 M, or 4.3 M NaCl in the presence of 50 ng/mL of the cell wall synthesis inhibitor caspofungin. Microscopy images were taken when the cultures reached an OD 600 of 0.5. Two independent experiments yielded similar data, and the representative images of one experiment are shown. The length of the scale bars is 20 µm. Inlays show representative examples of casponfungin-caused changed morphology. ( B ) Melanisation of H. werneckii progenitor and evolved strains grown in the presence of different solutes. Pre-cultures diluted to OD 600 0.5 were spotted at 10-fold serial dilutions on MEA plates without osmolyte or with sorbitol, glycerol, NaCl, or KCl as indicated. Images were taken after 9 days incubation, except for 1 M sorbitol (taken after 6 days) and 4.3 M NaCl (taken after 14 days). The experiment was performed in triplicate and the representative images are shown.

    Techniques Used: Microscopy, Incubation

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    Merck KGaA malt extract broth
    Malt Extract Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/malt extract broth/product/Merck KGaA
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    malt extract broth meb  (Merck KGaA)
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    Merck KGaA malt extract broth meb
    Malt Extract Broth Meb, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA malt extract broth medium meb
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