Journal: Journal of Fungi

Article Title: What’s New in Prevention of Invasive Fungal Diseases during Hospital Construction and Renovation Work: An Overview

doi: 10.3390/jof9020151

Figure Lengend Snippet: Methods used for collecting air and environmental samples, fungal identification, and measured total and specific airborne fungal levels during fungal outbreaks and infections associated with construction or renovation described in articles published from January 2015 to December 2022.

Article Snippet: Wirmann, L. et al., 2018 [ ] , The measuring apparatus MAS−100 (Merck Chemicals GmbH, Darmstadt, Germany) was used. The apparatus was placed 1m above ground level. For each sample, 500 L of air was collected on malt extract agar plates. The measuring head of MAS−100 was autoclaved between each sampling day. The agar plates were incubated at 50 °C for 48 h. , not performed , Samples that showed visible mold growth after the incubation period were examined microscopically to identify A. fumigatus . All A. fumigatus isolates were plated on to Sabouraud dextrose agar containing 4mg/L itraconazole to screen for azole resistance. Grown isolates were subjected to antifungal susceptibility testing for itraconazole, voriconazole, posaconazole, and isavuconazole, according to EUCAST standard 9.3. The cyp51A gene was sequenced for all isolates with elevated minimum inhibitory concentration against an azole, as described recently. All colonies identified microscopically as A. fumigatus were counted and documented as colony-forming units (CFU)/m 3 . Additionally, the number of colonies was corrected according to Feller. , not performed (only Aspergillus spp. concentrations were measured) , Mean concentrations of A. fumigatus spores did not differ significantly be-tween the three periods before (17.5 CFU/m³), during 30 (20.8 CFU/m³) ( p = 0.26), and after demolition (17.7 CFU/m³) ( p = 0.33). , N/A.

Techniques: Environmental Sampling, Sampling, Incubation, Microscopy, Concentration Assay, Modification, Infection, Bioburden Testing