Structured Review

Charles River Laboratories male c57bl 6 mice
Betaine supplementation alleviates plasma adiponectin reduction and 4-HNE increase in adipose tissue in HF diet mice. Male <t>C57BL/6</t> mice were fed HF diets with or without betaine [1% (wt/vol)] supplementation in the drinking water for 12 weeks. At last,
Male C57bl 6 Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/male c57bl 6 mice/product/Charles River Laboratories
Average 90 stars, based on 26 article reviews
Price from $9.99 to $1999.99
male c57bl 6 mice - by Bioz Stars, 2022-09
90/100 stars

Images

1) Product Images from "4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPAR\u03b3 and accelerating ubiquitin\u2013proteasome degradation"

Article Title: 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPAR\u03b3 and accelerating ubiquitin\u2013proteasome degradation

Journal: Molecular and cellular endocrinology

doi: 10.1016/j.mce.2011.10.027

Betaine supplementation alleviates plasma adiponectin reduction and 4-HNE increase in adipose tissue in HF diet mice. Male C57BL/6 mice were fed HF diets with or without betaine [1% (wt/vol)] supplementation in the drinking water for 12 weeks. At last,
Figure Legend Snippet: Betaine supplementation alleviates plasma adiponectin reduction and 4-HNE increase in adipose tissue in HF diet mice. Male C57BL/6 mice were fed HF diets with or without betaine [1% (wt/vol)] supplementation in the drinking water for 12 weeks. At last,

Techniques Used: Mouse Assay

Long-term high-fat diet feeding caused adiposity and decreased plasma adiponectin level, which is associated with increased oxidative stress in adipose tissue. Male C57BL/6 mice were fed with control and high-fat (HF) diets. Twelve weeks later, the epididymal
Figure Legend Snippet: Long-term high-fat diet feeding caused adiposity and decreased plasma adiponectin level, which is associated with increased oxidative stress in adipose tissue. Male C57BL/6 mice were fed with control and high-fat (HF) diets. Twelve weeks later, the epididymal

Techniques Used: Mouse Assay

2) Product Images from "Skin-stage Schistosomula of Schistosoma mansoni Produce an Apoptosis-inducing Factor That Can Cause Apoptosis of T Cells *"

Article Title: Skin-stage Schistosomula of Schistosoma mansoni Produce an Apoptosis-inducing Factor That Can Cause Apoptosis of T Cells *

Journal: The Journal of biological chemistry

doi: 10.1074/jbc.M201344200

Time courses of caspase-8 and caspase-3 protease activities 5 × 10 6 skin-draining lymph node cells from naive C57BL/6 mice were cultured with 60 μ g/ml ES products for 0, 8, 16, and 32 h. Cells were harvested at different time points, and caspase-8 or caspase-3 protease activity was examined by a colorimetric protease assay. Data presented are from one of two similar experiments.
Figure Legend Snippet: Time courses of caspase-8 and caspase-3 protease activities 5 × 10 6 skin-draining lymph node cells from naive C57BL/6 mice were cultured with 60 μ g/ml ES products for 0, 8, 16, and 32 h. Cells were harvested at different time points, and caspase-8 or caspase-3 protease activity was examined by a colorimetric protease assay. Data presented are from one of two similar experiments.

Techniques Used: Mouse Assay, Cell Culture, Activity Assay, Protease Assay

3) Product Images from "Angiotensin type 1 receptor antagonist losartan, reduces MPTP-induced degeneration of dopaminergic neurons in substantia nigra"

Article Title: Angiotensin type 1 receptor antagonist losartan, reduces MPTP-induced degeneration of dopaminergic neurons in substantia nigra

Journal: Molecular Neurodegeneration

doi: 10.1186/1750-1326-2-1

In vivo AT receptor profile of DA neurons in the substantia nigra pars compacta (SNpc) of adult C57BL/6 male mice. Coronal sections (40 μm) were stained for (A) AT 1 receptor (red) or (D) AT 2 receptor (red), (B, E) TH (DA neurons) (green) and (C, F) represent merged images. Scale bar equals to 200 μm.
Figure Legend Snippet: In vivo AT receptor profile of DA neurons in the substantia nigra pars compacta (SNpc) of adult C57BL/6 male mice. Coronal sections (40 μm) were stained for (A) AT 1 receptor (red) or (D) AT 2 receptor (red), (B, E) TH (DA neurons) (green) and (C, F) represent merged images. Scale bar equals to 200 μm.

Techniques Used: In Vivo, Mouse Assay, Staining

4) Product Images from "Transcribed ultraconserved noncoding RNAs (T-UCR) are involved in Barrett's esophagus carcinogenesis"

Article Title: Transcribed ultraconserved noncoding RNAs (T-UCR) are involved in Barrett's esophagus carcinogenesis

Journal: Oncotarget

doi:

T-UCR dysregulation is comparable in human disease and murine models Five male Wistar Han rats and 5 male C57BL/6 mice underwent “Kumagai-Hattori” esophago-gastroduodenal anastomosis and were sacrificed 52 weeks after surgery. Their esophageal mucosa was macroscopically reddened with small protruding lesions ( A , gross features representative of two surgically-treated rats). Areas of esophageal intestinal metaplasia (IM) were microdissected manually to assess T-UCR expression. An up-regulation of uc.158- ( B ), uc.472- ( C ), and uc.473- ( D ) was observed in the samples of IM from all three species considered.
Figure Legend Snippet: T-UCR dysregulation is comparable in human disease and murine models Five male Wistar Han rats and 5 male C57BL/6 mice underwent “Kumagai-Hattori” esophago-gastroduodenal anastomosis and were sacrificed 52 weeks after surgery. Their esophageal mucosa was macroscopically reddened with small protruding lesions ( A , gross features representative of two surgically-treated rats). Areas of esophageal intestinal metaplasia (IM) were microdissected manually to assess T-UCR expression. An up-regulation of uc.158- ( B ), uc.472- ( C ), and uc.473- ( D ) was observed in the samples of IM from all three species considered.

Techniques Used: Mouse Assay, Expressing

5) Product Images from "In Vivo Detection and Measurement of Aortic Aneurysm and Dissection in Mouse Models Using Microcomputed Tomography with Contrast Agent"

Article Title: In Vivo Detection and Measurement of Aortic Aneurysm and Dissection in Mouse Models Using Microcomputed Tomography with Contrast Agent

Journal: Contrast Media & Molecular Imaging

doi: 10.1155/2019/5940301

(a) Time course of contrast enhancement within the aorta of the three healthy C57BL/6 mice after a single tail vein injection of 100 μ l ExiTron nano 12000 per 25 g mouse. (b) Mean CT number (HU) at the second hour after a single tail vein injection of ExiTron nano 12000 at a dose of 75 μ l/25g, 100 μ l/25g, and 150 μ l/25 g per mouse, respectively. Mean CT number was measured by placing a region of interest within the aorta.
Figure Legend Snippet: (a) Time course of contrast enhancement within the aorta of the three healthy C57BL/6 mice after a single tail vein injection of 100 μ l ExiTron nano 12000 per 25 g mouse. (b) Mean CT number (HU) at the second hour after a single tail vein injection of ExiTron nano 12000 at a dose of 75 μ l/25g, 100 μ l/25g, and 150 μ l/25 g per mouse, respectively. Mean CT number was measured by placing a region of interest within the aorta.

Techniques Used: Mouse Assay, Injection

6) Product Images from "Hypergravity Load Modulates Acetaminophen Nephrotoxicity via Endoplasmic Reticulum Stress in Association with Hepatic microRNA-122 Expression"

Article Title: Hypergravity Load Modulates Acetaminophen Nephrotoxicity via Endoplasmic Reticulum Stress in Association with Hepatic microRNA-122 Expression

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms22094901

Increased kidney dysfunction in mice exposed to a single hypergravity load coupled with APAP treatment. ( A ) Schematic of hypergravity and APAP treatments. ( B ) TUNEL and H E staining of kidney sections. Male C57BL/6 mice were exposed to a single +9 Gx load for 1 h, followed by APAP treatment (500 mg/kg BW, i.p.), and sacrificed 6 h afterward. TUNEL and H E staining was performed for two samples per treatment condition (randomly selected from 6 samples). Scale bar: 100 μm. The arrows indicate TUNEL-positive cells. The asterisks in the H E staining images indicate desquamation of the tubular epithelium, and arrows indicate nuclear swelling and vacuolization of the tubular epithelium in necrotizing tubular epithelia. ( C ) Serum creatinine contents in the same mice as in panel B ( n = 6/group). ( D ) Immunoblots for pAKT and pCREB in renal medulla homogenates from the same mice as in panel B (representative blots are presented). Quantifications were conducted for six samples per group. ( E ) qRT-PCR assays for Kim-1 , Bax , and Bcl2 in the renal cortex homogenates. The data in C-E represent the mean ± SEM (statistical differences were determined via the one-way ANOVA; * p
Figure Legend Snippet: Increased kidney dysfunction in mice exposed to a single hypergravity load coupled with APAP treatment. ( A ) Schematic of hypergravity and APAP treatments. ( B ) TUNEL and H E staining of kidney sections. Male C57BL/6 mice were exposed to a single +9 Gx load for 1 h, followed by APAP treatment (500 mg/kg BW, i.p.), and sacrificed 6 h afterward. TUNEL and H E staining was performed for two samples per treatment condition (randomly selected from 6 samples). Scale bar: 100 μm. The arrows indicate TUNEL-positive cells. The asterisks in the H E staining images indicate desquamation of the tubular epithelium, and arrows indicate nuclear swelling and vacuolization of the tubular epithelium in necrotizing tubular epithelia. ( C ) Serum creatinine contents in the same mice as in panel B ( n = 6/group). ( D ) Immunoblots for pAKT and pCREB in renal medulla homogenates from the same mice as in panel B (representative blots are presented). Quantifications were conducted for six samples per group. ( E ) qRT-PCR assays for Kim-1 , Bax , and Bcl2 in the renal cortex homogenates. The data in C-E represent the mean ± SEM (statistical differences were determined via the one-way ANOVA; * p

Techniques Used: Mouse Assay, TUNEL Assay, Staining, Western Blot, Quantitative RT-PCR

Effects of hypergravity preconditioning on kidney dysfunction, cell survival marker expression, and ER stress induced by APAP treatment. ( A ) Schematic of hypergravity and APAP treatments. ( B ) Serum creatinine contents, ALT activities, and qRT-PCR assay results for Kim-1 in the renal cortex. Male C57BL/6 mice ( n = 5 for APAP-9 GX3 group, and n = 6 for the other groups) were subjected to a single +9 Gx load for 1 h (9 GX1) or multiple +9 Gx loads for 1 h on three consecutive days (9 GX3), followed by a single administration of APAP (500 mg/kg BW, i.p.); the mice were sacrificed 6 h afterward. ( C , D ) Immunoblots for pAKT and pCREB ( C ) and ER stress marker proteins ( D ). Assays were conducted on the same renal medulla homogenates as above ( n = 5 for the APAP-9 GX3 group; n = 6 for the other groups). ( E ) The network of genes linked to Nrf2 in the liver of mice treated with APAP and hypergravity. Blue boxes indicate representative cell-survival marker proteins. The data were reported as the mean ± SEM (statistical differences were determined via the one-way ANOVA; * p
Figure Legend Snippet: Effects of hypergravity preconditioning on kidney dysfunction, cell survival marker expression, and ER stress induced by APAP treatment. ( A ) Schematic of hypergravity and APAP treatments. ( B ) Serum creatinine contents, ALT activities, and qRT-PCR assay results for Kim-1 in the renal cortex. Male C57BL/6 mice ( n = 5 for APAP-9 GX3 group, and n = 6 for the other groups) were subjected to a single +9 Gx load for 1 h (9 GX1) or multiple +9 Gx loads for 1 h on three consecutive days (9 GX3), followed by a single administration of APAP (500 mg/kg BW, i.p.); the mice were sacrificed 6 h afterward. ( C , D ) Immunoblots for pAKT and pCREB ( C ) and ER stress marker proteins ( D ). Assays were conducted on the same renal medulla homogenates as above ( n = 5 for the APAP-9 GX3 group; n = 6 for the other groups). ( E ) The network of genes linked to Nrf2 in the liver of mice treated with APAP and hypergravity. Blue boxes indicate representative cell-survival marker proteins. The data were reported as the mean ± SEM (statistical differences were determined via the one-way ANOVA; * p

Techniques Used: Marker, Expressing, Quantitative RT-PCR, Mouse Assay, Western Blot

7) Product Images from "The p47phox- and NADPH oxidase organiser 1 (NOXO1)-dependent activation of NADPH oxidase 1 (NOX1) mediates endothelial nitric oxide synthase (eNOS) uncoupling and endothelial dysfunction in a streptozotocin-induced murine model of diabetes"

Article Title: The p47phox- and NADPH oxidase organiser 1 (NOXO1)-dependent activation of NADPH oxidase 1 (NOX1) mediates endothelial nitric oxide synthase (eNOS) uncoupling and endothelial dysfunction in a streptozotocin-induced murine model of diabetes

Journal: Diabetologia

doi: 10.1007/s00125-012-2557-6

Recoupling of eNOS in diabetes via restoration of DHFR. C57BL/6 mice were made diabetic by STZ injection (100 mg/kg per day for 3 days) and transfected with empty vector or pcDNA3.1- Dhfr plasmid ( a – d ). Oral administration with folic acid (15 mg/kg per day) was started 2 days prior to induction of diabetes with STZ (100 mg/kg per day for 3 days), and mice were fed with folic acid-containing food throughout the study period of 7 days ( e – h ). On day 7, blood glucose levels were determined and aortas were harvested for superoxide production using electron spin resonance. a Blood glucose levels. *p
Figure Legend Snippet: Recoupling of eNOS in diabetes via restoration of DHFR. C57BL/6 mice were made diabetic by STZ injection (100 mg/kg per day for 3 days) and transfected with empty vector or pcDNA3.1- Dhfr plasmid ( a – d ). Oral administration with folic acid (15 mg/kg per day) was started 2 days prior to induction of diabetes with STZ (100 mg/kg per day for 3 days), and mice were fed with folic acid-containing food throughout the study period of 7 days ( e – h ). On day 7, blood glucose levels were determined and aortas were harvested for superoxide production using electron spin resonance. a Blood glucose levels. *p

Techniques Used: Mouse Assay, Injection, Transfection, Plasmid Preparation, Electron Paramagnetic Resonance

NOX1, but not NOX4, mediates diabetic uncoupling of eNOS. C57BL/6 mice were made diabetic by STZ injection (100 mg/kg per day for 3 days) and transfected with control, Nox1 or Nox4 siRNA. On day 7, blood glucose levels were determined and aortas were harvested for L-NAME-sensitive superoxide detection using electron spin resonance. a Protein contents of NOX1 and NOX4 in siRNA-transfected diabetic mouse aortas. b Blood glucose levels. c , d eNOS uncoupling activity in scrambled siRNA, Nox1 or Nox4 siRNA-transfected diabetic mice. * p
Figure Legend Snippet: NOX1, but not NOX4, mediates diabetic uncoupling of eNOS. C57BL/6 mice were made diabetic by STZ injection (100 mg/kg per day for 3 days) and transfected with control, Nox1 or Nox4 siRNA. On day 7, blood glucose levels were determined and aortas were harvested for L-NAME-sensitive superoxide detection using electron spin resonance. a Protein contents of NOX1 and NOX4 in siRNA-transfected diabetic mouse aortas. b Blood glucose levels. c , d eNOS uncoupling activity in scrambled siRNA, Nox1 or Nox4 siRNA-transfected diabetic mice. * p

Techniques Used: Mouse Assay, Injection, Transfection, Electron Paramagnetic Resonance, Activity Assay

NOXO1-dependent NOX1 mediates diabetic uncoupling of eNOS. C57BL/6 and NOX1 − /y mice were made diabetic by STZ injection (100 mg/kg per day for 3 days) and transfected with control or Noxo1 siRNA as described in the Methods section. On day 7, blood glucose levels were determined and aortas were harvested for L-NAME-sensitive superoxide detection using electron spin resonance. a eNOS uncoupling activity in control siRNA or Noxo1 siRNA-transfected diabetic mice. b Protein content of NOXO1 in siRNA-transfected diabetic mouse aortas. c Blood glucose levels. * p
Figure Legend Snippet: NOXO1-dependent NOX1 mediates diabetic uncoupling of eNOS. C57BL/6 and NOX1 − /y mice were made diabetic by STZ injection (100 mg/kg per day for 3 days) and transfected with control or Noxo1 siRNA as described in the Methods section. On day 7, blood glucose levels were determined and aortas were harvested for L-NAME-sensitive superoxide detection using electron spin resonance. a eNOS uncoupling activity in control siRNA or Noxo1 siRNA-transfected diabetic mice. b Protein content of NOXO1 in siRNA-transfected diabetic mouse aortas. c Blood glucose levels. * p

Techniques Used: Mouse Assay, Injection, Transfection, Electron Paramagnetic Resonance, Activity Assay

Mitochondrion does not contribute to diabetic uncoupling of eNOS. C57BL/6 mice were fed the mitochondrial complex I inhibitor rotenone for 2 days prior to being made diabetic by STZ injection (100 mg/kg per day for 3 days), and throughout the study period of 7 days. Some diabetic mice were transfected with complex III siRNA as described in the Methods section. a Expression of Rieske subunit of mitochondrial complex III. b Circulating levels of rotenone in mice as determined by HPLC analysis (see Methods section). c L-NAME-sensitive superoxide production (eNOS uncoupling activity). d Blood glucose levels. * p
Figure Legend Snippet: Mitochondrion does not contribute to diabetic uncoupling of eNOS. C57BL/6 mice were fed the mitochondrial complex I inhibitor rotenone for 2 days prior to being made diabetic by STZ injection (100 mg/kg per day for 3 days), and throughout the study period of 7 days. Some diabetic mice were transfected with complex III siRNA as described in the Methods section. a Expression of Rieske subunit of mitochondrial complex III. b Circulating levels of rotenone in mice as determined by HPLC analysis (see Methods section). c L-NAME-sensitive superoxide production (eNOS uncoupling activity). d Blood glucose levels. * p

Techniques Used: Mouse Assay, Injection, Transfection, Expressing, High Performance Liquid Chromatography, Activity Assay

8) Product Images from "Inhibition of ERK1/2 Pathway Suppresses Adiponectin Secretion via Accelerating Protein Degradation by Ubiquitin-Proteasome System: Relevance to Obesity-related Adiponectin Decline"

Article Title: Inhibition of ERK1/2 Pathway Suppresses Adiponectin Secretion via Accelerating Protein Degradation by Ubiquitin-Proteasome System: Relevance to Obesity-related Adiponectin Decline

Journal: Metabolism: clinical and experimental

doi: 10.1016/j.metabol.2013.01.025

Obesity-related adiponectin decline is associated with suppressed ERK1/2 and AKT activation in adipose tissue. Male C57BL/6 mice were exposed to high-fat diet for eight weeks. (A) body weight; (B) epididymal fat mass; (C) adiponectin and PPAR-γ
Figure Legend Snippet: Obesity-related adiponectin decline is associated with suppressed ERK1/2 and AKT activation in adipose tissue. Male C57BL/6 mice were exposed to high-fat diet for eight weeks. (A) body weight; (B) epididymal fat mass; (C) adiponectin and PPAR-γ

Techniques Used: Activation Assay, Mouse Assay

9) Product Images from "Divergent Leptin Signaling in Proglucagon Neurons of the Nucleus of the Solitary Tract in Mice and Rats"

Article Title: Divergent Leptin Signaling in Proglucagon Neurons of the Nucleus of the Solitary Tract in Mice and Rats

Journal:

doi: 10.1210/en.2007-0633

Anatomical localization of leptin-responsive neurons in the NTS of rats and mice. Male Sprague Dawley rats and C57BL/6 mice were injected with leptin ip or PBS for 45 min. Series of coronal hindbrain sections were subjected to P-STAT3 DAB IHC. Shown are
Figure Legend Snippet: Anatomical localization of leptin-responsive neurons in the NTS of rats and mice. Male Sprague Dawley rats and C57BL/6 mice were injected with leptin ip or PBS for 45 min. Series of coronal hindbrain sections were subjected to P-STAT3 DAB IHC. Shown are

Techniques Used: Mouse Assay, Injection, Immunohistochemistry

Leptin induces P-STAT3 in 100% GLP-1-positive cells in the NTS of mice but not rats. Male Sprague Dawley rats or C57BL/6 mice were injected with leptin ip for 45 min. Series of coronal brain sections were subjected to combined P-STAT3 DAB and GLP-1 fluorescence
Figure Legend Snippet: Leptin induces P-STAT3 in 100% GLP-1-positive cells in the NTS of mice but not rats. Male Sprague Dawley rats or C57BL/6 mice were injected with leptin ip for 45 min. Series of coronal brain sections were subjected to combined P-STAT3 DAB and GLP-1 fluorescence

Techniques Used: Mouse Assay, Injection, Fluorescence

Proglucagon mRNA is regulated by fasting and leptin in the NTS of mice but not rats. Male Sprague Dawley rats and C57BL/6 mice were fed ad libitum and treated with vehicle (PBS), fasted (48 h) and treated with vehicle (PBS), or fasted (48 h) and treated
Figure Legend Snippet: Proglucagon mRNA is regulated by fasting and leptin in the NTS of mice but not rats. Male Sprague Dawley rats and C57BL/6 mice were fed ad libitum and treated with vehicle (PBS), fasted (48 h) and treated with vehicle (PBS), or fasted (48 h) and treated

Techniques Used: Mouse Assay

10) Product Images from "The effects of graded levels of calorie restriction: II. Impact of short term calorie and protein restriction on circulating hormone levels, glucose homeostasis and oxidative stress in male C57BL/6 mice"

Article Title: The effects of graded levels of calorie restriction: II. Impact of short term calorie and protein restriction on circulating hormone levels, glucose homeostasis and oxidative stress in male C57BL/6 mice

Journal: Oncotarget

doi:

Protein restriction (PR) did not impact changes in oxidative stress biomarkers in the liver Antioxidant activity in the liver of a. catalase, b. superoxide dismutase (SOD), and c. glutathione peroxidase (GPx) was unchanged as was d. antioxidant capacity in the blood measured by OxyAdsorbent test (OxyD) following 3 months of graded PR. e. Protein damage as measured by protein carbonyls in the liver and f. reactive oxygen metabolites (dROMs) were not affected by PR reduced by 20, 30 or 40% (20PR, 30PR and 40PR) compared to 12 hr ad libitum (12AL) fed C57BL/6 male mice. Data displayed as mean ± sem and shown on the same axis as Figures 5 and 6 for ease of comparison of calorie restriction (CR) and PR.
Figure Legend Snippet: Protein restriction (PR) did not impact changes in oxidative stress biomarkers in the liver Antioxidant activity in the liver of a. catalase, b. superoxide dismutase (SOD), and c. glutathione peroxidase (GPx) was unchanged as was d. antioxidant capacity in the blood measured by OxyAdsorbent test (OxyD) following 3 months of graded PR. e. Protein damage as measured by protein carbonyls in the liver and f. reactive oxygen metabolites (dROMs) were not affected by PR reduced by 20, 30 or 40% (20PR, 30PR and 40PR) compared to 12 hr ad libitum (12AL) fed C57BL/6 male mice. Data displayed as mean ± sem and shown on the same axis as Figures 5 and 6 for ease of comparison of calorie restriction (CR) and PR.

Techniques Used: Antioxidant Activity Assay, Mouse Assay

Hormonal changes measured in male C57BL/6 mice following protein restriction (PR) PR at 20, 30 and 40% of ad libitum (AL) intake (20, 30, 40PR) does not affect circulating levels of a. leptin, c. insulin or d. insulin growth factor-1 (IGF-1) measured after 3 months of PR. 12AL depicts control animals fed during 12 hours of darkness. The strong relationship between fat mass and leptin are shown in b. Data presented as mean ± sem. To illustrate differences between calorie restriction (CR) and PR, results are shown on the same axis as Figure 1 .
Figure Legend Snippet: Hormonal changes measured in male C57BL/6 mice following protein restriction (PR) PR at 20, 30 and 40% of ad libitum (AL) intake (20, 30, 40PR) does not affect circulating levels of a. leptin, c. insulin or d. insulin growth factor-1 (IGF-1) measured after 3 months of PR. 12AL depicts control animals fed during 12 hours of darkness. The strong relationship between fat mass and leptin are shown in b. Data presented as mean ± sem. To illustrate differences between calorie restriction (CR) and PR, results are shown on the same axis as Figure 1 .

Techniques Used: Mouse Assay

Hormonal changes measured in male C57BL/6 mice following calorie restriction (CR) Mice were fed 12 or 24 hrs ad libitum (12AL or 24AL) or calorie restricted (CR) by 10, 20, 30 or 40% (10CR, 20CR, 30CR and 40CR) for 3 months. Circulating levels of a. leptin, c. tumor necrosis factor (TNF)-α, d. insulin-like growth factor (IGF-1) and f. insulin. Significant hormonal relationships are shown between b. fat mass and leptin, e. structural tissues and IGF-1, g. vital organs and insulin and h. liver and insulin. Results are expressed as mean ± sem. Different letters denote significant differences between treatment groups.
Figure Legend Snippet: Hormonal changes measured in male C57BL/6 mice following calorie restriction (CR) Mice were fed 12 or 24 hrs ad libitum (12AL or 24AL) or calorie restricted (CR) by 10, 20, 30 or 40% (10CR, 20CR, 30CR and 40CR) for 3 months. Circulating levels of a. leptin, c. tumor necrosis factor (TNF)-α, d. insulin-like growth factor (IGF-1) and f. insulin. Significant hormonal relationships are shown between b. fat mass and leptin, e. structural tissues and IGF-1, g. vital organs and insulin and h. liver and insulin. Results are expressed as mean ± sem. Different letters denote significant differences between treatment groups.

Techniques Used: Mouse Assay

11) Product Images from "Natural History of Age-Related Retinal Lesions That Precede AMD in Mice Fed High or Low Glycemic Index Diets"

Article Title: Natural History of Age-Related Retinal Lesions That Precede AMD in Mice Fed High or Low Glycemic Index Diets

Journal: Investigative Ophthalmology & Visual Science

doi: 10.1167/iovs.11-8545

Retinal lesions are more advanced in C57BL/6 mice that were older and that consumed the high GI diet. Electron micrographs of ( A ) 17-month-old high GI-fed, ( B ) 23.5-month-old high GI-fed, ( C ) 17-month-old low GI-fed, and ( D ) 23.5-month-old low GI-fed mice are shown. Note larger, more advanced BLDs, greater loss of BI, and vacuolization of cytoplasm in B than in A , C , and D . BI, basal infolding; BrM, Bruch's membrane (knobbed lines indicate approximate thickness of Bruch's Membrane); FD, fibrillar deposit; MD, BLD-associated membranous debris; RPE, retinal pigment epithelium; V, vacuoles; +, loss of basal infoldings. Scale bar, 1 μm.
Figure Legend Snippet: Retinal lesions are more advanced in C57BL/6 mice that were older and that consumed the high GI diet. Electron micrographs of ( A ) 17-month-old high GI-fed, ( B ) 23.5-month-old high GI-fed, ( C ) 17-month-old low GI-fed, and ( D ) 23.5-month-old low GI-fed mice are shown. Note larger, more advanced BLDs, greater loss of BI, and vacuolization of cytoplasm in B than in A , C , and D . BI, basal infolding; BrM, Bruch's membrane (knobbed lines indicate approximate thickness of Bruch's Membrane); FD, fibrillar deposit; MD, BLD-associated membranous debris; RPE, retinal pigment epithelium; V, vacuoles; +, loss of basal infoldings. Scale bar, 1 μm.

Techniques Used: Mouse Assay

Comparable levels of retinal lesions observed in 17-month-old high GI-fed C57BL/6 mice in the absence or presence of HQ. Electron micrographs of retinas from mice fed high GI diets in the absence ( A ) or presence ( B ) of HQ. BLD, basal laminar deposit; BrM, Bruch's membrane (knobbed lines indicate thickness of Bruch's membrane); LIPO, lipofuscin-like granule; OCL, outer collagenous layer deposit; RPE, retinal pigment epithelium; V, vacuole; +, loss of basal infoldings. Scale bar, 1 μm. Mean values for high GI-fed mice in the absence ( blue bars ) and presence ( green bars ) of HQ are shown for frequency of basal laminar deposits ( C ) and severity of basal laminar deposits ( D ). Error bars represent SEM. ** P
Figure Legend Snippet: Comparable levels of retinal lesions observed in 17-month-old high GI-fed C57BL/6 mice in the absence or presence of HQ. Electron micrographs of retinas from mice fed high GI diets in the absence ( A ) or presence ( B ) of HQ. BLD, basal laminar deposit; BrM, Bruch's membrane (knobbed lines indicate thickness of Bruch's membrane); LIPO, lipofuscin-like granule; OCL, outer collagenous layer deposit; RPE, retinal pigment epithelium; V, vacuole; +, loss of basal infoldings. Scale bar, 1 μm. Mean values for high GI-fed mice in the absence ( blue bars ) and presence ( green bars ) of HQ are shown for frequency of basal laminar deposits ( C ) and severity of basal laminar deposits ( D ). Error bars represent SEM. ** P

Techniques Used: Mouse Assay

12) Product Images from "Ivabradine reduces heart rate while preserving metabolic fluxes and energy status of healthy normoxic working hearts"

Article Title: Ivabradine reduces heart rate while preserving metabolic fluxes and energy status of healthy normoxic working hearts

Journal: American journal of physiology. Heart and circulatory physiology

doi: 10.1152/ajpheart.01034.2010

Impact of ivabradine (IVA) on heart rate ( A ), stroke volume ( B ), coronary flow expressed per minute ( C ) and coronary flow expressed per beat ( D ) of isolated working C57Bl/6 mouse hearts perfused in the absence (control) or in the presence of 3 μM
Figure Legend Snippet: Impact of ivabradine (IVA) on heart rate ( A ), stroke volume ( B ), coronary flow expressed per minute ( C ) and coronary flow expressed per beat ( D ) of isolated working C57Bl/6 mouse hearts perfused in the absence (control) or in the presence of 3 μM

Techniques Used: Flow Cytometry, Isolation

Relative contribution of glucose and oleate to mitochondrial acetyl-CoA formation for citrate synthesis and rates of glycolysis in working C57Bl/6 mouse hearts perfused in the absence (control) or in the presence of IVA with or without atrial pacing.
Figure Legend Snippet: Relative contribution of glucose and oleate to mitochondrial acetyl-CoA formation for citrate synthesis and rates of glycolysis in working C57Bl/6 mouse hearts perfused in the absence (control) or in the presence of IVA with or without atrial pacing.

Techniques Used:

Energy production in working C57Bl/6 mouse hearts perfused in the absence (control) or in the presence of IVA with or without atrial pacing. Data are expressed as means ± SE of 4 –5 hearts. Rates of ATP production in cytosol and mitochondria
Figure Legend Snippet: Energy production in working C57Bl/6 mouse hearts perfused in the absence (control) or in the presence of IVA with or without atrial pacing. Data are expressed as means ± SE of 4 –5 hearts. Rates of ATP production in cytosol and mitochondria

Techniques Used:

Impact of 20 and 40 μM metoprolol on heart rate ( A ), contractility ( B ), cardiac output and coronary flow ( C ), and stroke volume ( D ) of isolated working C57Bl/6 mouse hearts. Data are expressed as means ± SE of 3 hearts. $ P
Figure Legend Snippet: Impact of 20 and 40 μM metoprolol on heart rate ( A ), contractility ( B ), cardiac output and coronary flow ( C ), and stroke volume ( D ) of isolated working C57Bl/6 mouse hearts. Data are expressed as means ± SE of 3 hearts. $ P

Techniques Used: Flow Cytometry, Isolation

13) Product Images from "Nrf2- and PPAR?-Mediated Regulation of Hepatic Mrp Transporters after Exposure to Perfluorooctanoic Acid and Perfluorodecanoic Acid"

Article Title: Nrf2- and PPAR?-Mediated Regulation of Hepatic Mrp Transporters after Exposure to Perfluorooctanoic Acid and Perfluorodecanoic Acid

Journal:

doi: 10.1093/toxsci/kfn177

Mrps are induced by various PFCAs. Expression of Mrp3 and Mrp4 mRNA in liver 48 h after treatment with vehicle or 80 mg/kg PFDA or PFOA. Total RNA from livers of chemically treated male C57BL/6 mice ( n = 5 per treatment) was analyzed by the branched DNA
Figure Legend Snippet: Mrps are induced by various PFCAs. Expression of Mrp3 and Mrp4 mRNA in liver 48 h after treatment with vehicle or 80 mg/kg PFDA or PFOA. Total RNA from livers of chemically treated male C57BL/6 mice ( n = 5 per treatment) was analyzed by the branched DNA

Techniques Used: Expressing, Mouse Assay

14) Product Images from "Metabolic Effects of Social Isolation in Adult C57BL/6 Mice"

Article Title: Metabolic Effects of Social Isolation in Adult C57BL/6 Mice

Journal: International Scholarly Research Notices

doi: 10.1155/2014/690950

Representative sections of H E stained adipose tissue showing WATi and WATr adipocytes were enlarged in SI mice ((d) and (f), resp.) compared to control C57BL/6 mice ((a) and (c), resp.). In contrast, WATe adipocytes were comparable in size between the two groups ((b) and (e)). Scale bar = 100 μ m.
Figure Legend Snippet: Representative sections of H E stained adipose tissue showing WATi and WATr adipocytes were enlarged in SI mice ((d) and (f), resp.) compared to control C57BL/6 mice ((a) and (c), resp.). In contrast, WATe adipocytes were comparable in size between the two groups ((b) and (e)). Scale bar = 100 μ m.

Techniques Used: Staining, Mouse Assay

15) Product Images from "Autophagy Induction and Accumulation of Phosphorylated Tau in the Hippocampus and Prefrontal Cortex of Adult C57BL/6 Mice Subjected to Adolescent Fluoxetine Treatment"

Article Title: Autophagy Induction and Accumulation of Phosphorylated Tau in the Hippocampus and Prefrontal Cortex of Adult C57BL/6 Mice Subjected to Adolescent Fluoxetine Treatment

Journal: Journal of Alzheimer's disease : JAD

doi: 10.3233/JAD-210475

Experimental design. Male C57BL/6 mice (N = 20; 10/group) were administered with vehicle (VEH; distilled water) or fluoxetine (FLX) for 15 consecutive days during the adolescent period of development (Postnatal Day [PD] 35–49), followed by a 21-day break without FLX exposure (washout period). Tissue was collected on PD70 (adulthood) for western blotting analysis.
Figure Legend Snippet: Experimental design. Male C57BL/6 mice (N = 20; 10/group) were administered with vehicle (VEH; distilled water) or fluoxetine (FLX) for 15 consecutive days during the adolescent period of development (Postnatal Day [PD] 35–49), followed by a 21-day break without FLX exposure (washout period). Tissue was collected on PD70 (adulthood) for western blotting analysis.

Techniques Used: Mouse Assay, Western Blot

16) Product Images from "Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect of betaine in a mouse model of alcoholic liver disease"

Article Title: Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect of betaine in a mouse model of alcoholic liver disease

Journal: British Journal of Pharmacology

doi: 10.1111/bph.12765

Betaine supplementation alleviates alcohol-induced inhibition of PP2A and activation of HSL in adipose tissue. C57BL/6 mice were fed a control or alcohol-containing diet with/without betaine supplementation (0.5%, w v −1 ) for 5 weeks. Total protein extracts from epididymal fat pads were prepared thereafter. Forty micrograms of protein was subjected to Western blot analysis for (methylated) PP2A C subunit and HSL phosphorylation using specific antibodies. (A) PP2A activity in epididymal fat pad. (B) Epididymal adipose tissue PP2A C subunit methylation. (C) Epididymal adipose tissue HSL activity. Data are means ± SD ( n = 8 for PF, BT/PF and BT/AF; n = 6 for AF). Bars with different letters differ significantly ( P
Figure Legend Snippet: Betaine supplementation alleviates alcohol-induced inhibition of PP2A and activation of HSL in adipose tissue. C57BL/6 mice were fed a control or alcohol-containing diet with/without betaine supplementation (0.5%, w v −1 ) for 5 weeks. Total protein extracts from epididymal fat pads were prepared thereafter. Forty micrograms of protein was subjected to Western blot analysis for (methylated) PP2A C subunit and HSL phosphorylation using specific antibodies. (A) PP2A activity in epididymal fat pad. (B) Epididymal adipose tissue PP2A C subunit methylation. (C) Epididymal adipose tissue HSL activity. Data are means ± SD ( n = 8 for PF, BT/PF and BT/AF; n = 6 for AF). Bars with different letters differ significantly ( P

Techniques Used: Inhibition, Activation Assay, Mouse Assay, Western Blot, Methylation, Activity Assay

Betaine supplementation alleviates alcohol-induced fatty liver and liver injury. C57BL/6 mice were fed with control or alcohol-containing diet with/without betaine supplementation (0.5%, w v −1 ) for 5 weeks. (A) Changes in liver-to-body weight (BW). (B) Changes in the liver triglyceride content. (C) Changes in plasma ALT levels. (D) Changes in plasma AST levels. Data are means ± SD ( n = 8 for PF, BT/PF and BT/AF; n = 6 for AF). Bars with different letters differ significantly ( P
Figure Legend Snippet: Betaine supplementation alleviates alcohol-induced fatty liver and liver injury. C57BL/6 mice were fed with control or alcohol-containing diet with/without betaine supplementation (0.5%, w v −1 ) for 5 weeks. (A) Changes in liver-to-body weight (BW). (B) Changes in the liver triglyceride content. (C) Changes in plasma ALT levels. (D) Changes in plasma AST levels. Data are means ± SD ( n = 8 for PF, BT/PF and BT/AF; n = 6 for AF). Bars with different letters differ significantly ( P

Techniques Used: Mouse Assay, AST Assay

Betaine supplementation corrects the abnormal methionine metabolism in adipose tissue induced by chronic alcohol exposure. C57BL/6 mice were fed a control or alcohol-containing diet with/without betaine supplementation (0.5%, w v −1 ) for 5 weeks. (A) Intracellular methionine metabolic pathway. (B) SAM levels in epididymal fat pads. (C) SAH levels in epididymal fat pads. (D) Epididymal adipose tissue SAM:SAH ratio. (E) Homocysteine levels in epididymal fat pads. (F) BHMT gene expression and protein in epididymal fat pads. Data are means ± SD ( n = 8 for PF, BT/PF and BT/AF; n = 6 for AF). Bars with different letters differ significantly ( P
Figure Legend Snippet: Betaine supplementation corrects the abnormal methionine metabolism in adipose tissue induced by chronic alcohol exposure. C57BL/6 mice were fed a control or alcohol-containing diet with/without betaine supplementation (0.5%, w v −1 ) for 5 weeks. (A) Intracellular methionine metabolic pathway. (B) SAM levels in epididymal fat pads. (C) SAH levels in epididymal fat pads. (D) Epididymal adipose tissue SAM:SAH ratio. (E) Homocysteine levels in epididymal fat pads. (F) BHMT gene expression and protein in epididymal fat pads. Data are means ± SD ( n = 8 for PF, BT/PF and BT/AF; n = 6 for AF). Bars with different letters differ significantly ( P

Techniques Used: Mouse Assay, Expressing

Betaine supplementation rectifies alcohol-induced adipose tissue dysfunction. C57BL/6 mice were fed a control or alcohol-containing diet with/without betaine supplementation (0.5%, w v −1 ) for 5 weeks. (A) Epididymal fat pad weights. (B) H E staining of epididymal adipose tissue shows that betaine supplementation prevents the reduction in adipocyte size induced by chronic alcohol exposure. (C) Plasma glycerol levels. (D) Plasma non-esterified fatty acid levels. Data are means ± SD ( n = 8 for PF, BT/PF and BT/AF; n = 6 for AF). Bars with different letters differ significantly ( P
Figure Legend Snippet: Betaine supplementation rectifies alcohol-induced adipose tissue dysfunction. C57BL/6 mice were fed a control or alcohol-containing diet with/without betaine supplementation (0.5%, w v −1 ) for 5 weeks. (A) Epididymal fat pad weights. (B) H E staining of epididymal adipose tissue shows that betaine supplementation prevents the reduction in adipocyte size induced by chronic alcohol exposure. (C) Plasma glycerol levels. (D) Plasma non-esterified fatty acid levels. Data are means ± SD ( n = 8 for PF, BT/PF and BT/AF; n = 6 for AF). Bars with different letters differ significantly ( P

Techniques Used: Mouse Assay, Staining

17) Product Images from "Selective NLRP3 (Pyrin Domain–Containing Protein 3) Inflammasome Inhibitor Reduces Brain Injury After Intracerebral Hemorrhage"

Article Title: Selective NLRP3 (Pyrin Domain–Containing Protein 3) Inflammasome Inhibitor Reduces Brain Injury After Intracerebral Hemorrhage

Journal: Stroke

doi: 10.1161/STROKEAHA.117.018904

MCC950 attenuates brain injury and improves long-term outcome after intracerebral hemorrhage (ICH). ICH was induced in C57BL/6 mice by injection of autologous blood or collagenase. A , Flow chart illustrates MCC950 administration and experimental design. Mice received daily intraperitoneal (IP) injections of MCC950 (10 mg/kg) or an equal volume of phosphate-buffered saline (PBS) vehicle for 3 consecutive days starting immediately after ICH induction. B , Neurological tests were performed to evaluate the motor, sensory, and balance functions in mice receiving vehicle or MCC950 at days 1 and 3 after injection of autologous blood ( left ) or collagenase ( right ). C , T2-weighted image (T2WI) sequences were scanned to assess lesion volume at day 3 after ICH induced by injection of autologous blood ( left ) or collagenase ( right ), as outlined in red. Susceptibility-weighted sequences were assessed for hematoma lesion volume, visible in yellow regions. Quantification of lesion volume and perihematomal edema in mice receiving MCC950 or vehicle at day 3 after ICH induced by injection of autologous blood ( left ) or collagenase ( right ). n=8 mice per group. D , Mice received vehicle or MCC950 at a dose of 10 mg/kg by intraperitoneal injection. The assessments of modified Neurological Severity Score (mNSS) score and corner test were performed at days 7, 14, and 28 after ICH induced by injection of collagenase. n=10 per group. Data are presented as mean±SD. * P
Figure Legend Snippet: MCC950 attenuates brain injury and improves long-term outcome after intracerebral hemorrhage (ICH). ICH was induced in C57BL/6 mice by injection of autologous blood or collagenase. A , Flow chart illustrates MCC950 administration and experimental design. Mice received daily intraperitoneal (IP) injections of MCC950 (10 mg/kg) or an equal volume of phosphate-buffered saline (PBS) vehicle for 3 consecutive days starting immediately after ICH induction. B , Neurological tests were performed to evaluate the motor, sensory, and balance functions in mice receiving vehicle or MCC950 at days 1 and 3 after injection of autologous blood ( left ) or collagenase ( right ). C , T2-weighted image (T2WI) sequences were scanned to assess lesion volume at day 3 after ICH induced by injection of autologous blood ( left ) or collagenase ( right ), as outlined in red. Susceptibility-weighted sequences were assessed for hematoma lesion volume, visible in yellow regions. Quantification of lesion volume and perihematomal edema in mice receiving MCC950 or vehicle at day 3 after ICH induced by injection of autologous blood ( left ) or collagenase ( right ). n=8 mice per group. D , Mice received vehicle or MCC950 at a dose of 10 mg/kg by intraperitoneal injection. The assessments of modified Neurological Severity Score (mNSS) score and corner test were performed at days 7, 14, and 28 after ICH induced by injection of collagenase. n=10 per group. Data are presented as mean±SD. * P

Techniques Used: Mouse Assay, Injection, Flow Cytometry, Modification

MCC950 treatment preserves blood–brain barrier (BBB) integrity and reduces cell death after intracerebral hemorrhage (ICH). ICH was induced in C57BL/6 mice by injection of autologous blood. Mice received daily intraperitoneal (IP) injections of MCC950 (10 mg/kg) or an equal volume of vehicle for 3 consecutive days starting immediately after ICH induction. At day 3 after ICH, brain tissues were prepared for measurements of BBB integrity and cell death. A , Histology images show that MCC950 decreased Evens Blue dye leakage compared with vehicle (phosphate-buffered saline [PBS]) treatment. B , Western blot images show expression of the tight junction proteins, claudin-5, and ZO-1, in the brains of ICH mice receiving MCC950 or vehicle. C , Flow cytometry plots and summarized results show percentages of Annexin V + cells in the brains of ICH mice receiving MCC950 or vehicle. n=6 mice per group. Data are presented as mean±SD. * P
Figure Legend Snippet: MCC950 treatment preserves blood–brain barrier (BBB) integrity and reduces cell death after intracerebral hemorrhage (ICH). ICH was induced in C57BL/6 mice by injection of autologous blood. Mice received daily intraperitoneal (IP) injections of MCC950 (10 mg/kg) or an equal volume of vehicle for 3 consecutive days starting immediately after ICH induction. At day 3 after ICH, brain tissues were prepared for measurements of BBB integrity and cell death. A , Histology images show that MCC950 decreased Evens Blue dye leakage compared with vehicle (phosphate-buffered saline [PBS]) treatment. B , Western blot images show expression of the tight junction proteins, claudin-5, and ZO-1, in the brains of ICH mice receiving MCC950 or vehicle. C , Flow cytometry plots and summarized results show percentages of Annexin V + cells in the brains of ICH mice receiving MCC950 or vehicle. n=6 mice per group. Data are presented as mean±SD. * P

Techniques Used: Mouse Assay, Injection, Western Blot, Expressing, Flow Cytometry, Cytometry

Microglia contribute to the benefit of MCC950 after intracerebral hemorrhage (ICH). A , Schematic diagram illustrates drug administration and experimental design. C57BL/6 mice received oral gavage of PLX3397 (40 mg/kg) for 21 days and continuously until the experiment ended. ICH was induced in PLX3397-treated mice by injection of autologous blood. Thereafter, these mice received daily injections of MCC950 (10 mg/kg, intraperitoneal [IP]) or an equal volume of vehicle for 3 consecutive days starting immediately after ICH induction. At day 3 after ICH, neurodeficits and brain water content were assessed. B , Quantification of modified Neurological Severity Score (mNSS) and corner-turning test scores in indicated groups of ICH mice. C , Quantification of brain water content in indicated groups of ICH mice. n=6 per group. Data are presented as mean±SD.
Figure Legend Snippet: Microglia contribute to the benefit of MCC950 after intracerebral hemorrhage (ICH). A , Schematic diagram illustrates drug administration and experimental design. C57BL/6 mice received oral gavage of PLX3397 (40 mg/kg) for 21 days and continuously until the experiment ended. ICH was induced in PLX3397-treated mice by injection of autologous blood. Thereafter, these mice received daily injections of MCC950 (10 mg/kg, intraperitoneal [IP]) or an equal volume of vehicle for 3 consecutive days starting immediately after ICH induction. At day 3 after ICH, neurodeficits and brain water content were assessed. B , Quantification of modified Neurological Severity Score (mNSS) and corner-turning test scores in indicated groups of ICH mice. C , Quantification of brain water content in indicated groups of ICH mice. n=6 per group. Data are presented as mean±SD.

Techniques Used: Mouse Assay, Injection, Modification

MCC950 reduces leukocyte infiltration and microglia production of proinflammatory factors after intracerebral hemorrhage (ICH). ICH was induced in C57BL/6 mice by injection of autologous blood. Mice received daily intraperitoneal (IP) injections of MCC950 (10 mg/kg) or an equal volume of vehicle for 3 consecutive days starting immediately after ICH induction. At day 3 after ICH, immune cells were isolated from brain tissues of ICH mice receiving MCC950 or vehicle. A , Gating strategy of brain-infiltrating immune cells including CD4 + T cells (CD3 + CD4 + ), CD8 + T cells (CD3 + CD8 + ), B cells (CD3 − CD19 + ), NK cells (CD3 − NK1.1 + ), monocyte/macrophages (CD11b + CD45 high Ly6C + ), neutrophils (CD11b + CD45 high Ly6G + ), and microglia (CD11b + CD45 int ) and their expression of IL (interleukin)-6, TNF-α (tumor necrosis factor-α), TGF-β (transforming growth factor-β), and IL-10. FMO, fluorescence minus one. B , Data points show counts of brain-infiltrating leukocytes in the brains of ICH mice receiving indicated treatment. C , Data points show counts of microglia in the brains of ICH mice receiving indicated treatment. D , Data points show the counts of microglia expressing IL-6, TNF-α, TGF-β, and IL-10 in the brains of ICH mice receiving indicated treatment. n=6 mice per group. Data are presented as mean±SD. * P
Figure Legend Snippet: MCC950 reduces leukocyte infiltration and microglia production of proinflammatory factors after intracerebral hemorrhage (ICH). ICH was induced in C57BL/6 mice by injection of autologous blood. Mice received daily intraperitoneal (IP) injections of MCC950 (10 mg/kg) or an equal volume of vehicle for 3 consecutive days starting immediately after ICH induction. At day 3 after ICH, immune cells were isolated from brain tissues of ICH mice receiving MCC950 or vehicle. A , Gating strategy of brain-infiltrating immune cells including CD4 + T cells (CD3 + CD4 + ), CD8 + T cells (CD3 + CD8 + ), B cells (CD3 − CD19 + ), NK cells (CD3 − NK1.1 + ), monocyte/macrophages (CD11b + CD45 high Ly6C + ), neutrophils (CD11b + CD45 high Ly6G + ), and microglia (CD11b + CD45 int ) and their expression of IL (interleukin)-6, TNF-α (tumor necrosis factor-α), TGF-β (transforming growth factor-β), and IL-10. FMO, fluorescence minus one. B , Data points show counts of brain-infiltrating leukocytes in the brains of ICH mice receiving indicated treatment. C , Data points show counts of microglia in the brains of ICH mice receiving indicated treatment. D , Data points show the counts of microglia expressing IL-6, TNF-α, TGF-β, and IL-10 in the brains of ICH mice receiving indicated treatment. n=6 mice per group. Data are presented as mean±SD. * P

Techniques Used: Mouse Assay, Injection, Isolation, Expressing, Fluorescence

MCC950 inhibits NOD-like receptor (NLR) family, NLRP3 (pyrin domain-containing protein 3) inflammasome activation and IL (interleukin)-1β expression. Intracerebral hemorrhage (ICH) was induced in C57BL/6 mice by injection of autologous blood. Mice received daily intraperitoneal (IP) injections of MCC950 (10 mg/kg) or an equal volume of vehicle for 3 consecutive days starting immediately after ICH induction. At day 3 after ICH or sham surgery, brain tissues were harvested for the extraction of mRNA and protein. A , The mRNA expression levels of the NLRP3 inflammasome components and IL-1β after ICH. B , Western blot images show the expression of NLRP3, IL-1β, and caspase-1 in the brains of indicated groups of mice receiving sham plus vehicle, ICH plus vehicle, or ICH plus MCC950. C , Data points show the protein expression levels of NLRP3, IL-1β, and caspase-1 in the brains of indicated groups of mice receiving sham plus vehicle, ICH plus vehicle, or ICH plus MCC950. n=6 mice per group. Data are presented as mean±SD. * P
Figure Legend Snippet: MCC950 inhibits NOD-like receptor (NLR) family, NLRP3 (pyrin domain-containing protein 3) inflammasome activation and IL (interleukin)-1β expression. Intracerebral hemorrhage (ICH) was induced in C57BL/6 mice by injection of autologous blood. Mice received daily intraperitoneal (IP) injections of MCC950 (10 mg/kg) or an equal volume of vehicle for 3 consecutive days starting immediately after ICH induction. At day 3 after ICH or sham surgery, brain tissues were harvested for the extraction of mRNA and protein. A , The mRNA expression levels of the NLRP3 inflammasome components and IL-1β after ICH. B , Western blot images show the expression of NLRP3, IL-1β, and caspase-1 in the brains of indicated groups of mice receiving sham plus vehicle, ICH plus vehicle, or ICH plus MCC950. C , Data points show the protein expression levels of NLRP3, IL-1β, and caspase-1 in the brains of indicated groups of mice receiving sham plus vehicle, ICH plus vehicle, or ICH plus MCC950. n=6 mice per group. Data are presented as mean±SD. * P

Techniques Used: Activation Assay, Expressing, Mouse Assay, Injection, Western Blot

Gr-1 + myeloid cells contribute to the benefit of MCC950 after intracerebral hemorrhage (ICH). A , Schematic diagram illustrates drug administration and experimental design. C57BL/6 mice received anti–Gr-1 mAb 1 day before and 1 day after ICH surgery. ICH was induced by injection of autologous blood. Thereafter, these mice received daily injections of MCC950 (10 mg/kg, intraperitoneal [IP]) or an equal volume of vehicle for 3 consecutive days starting immediately after ICH induction. At day 3 after ICH, neurodeficits and brain water content were assessed. B , Data points show the counts of circulating Gr-1 + cells in mice before ICH induction, or mice receiving anti–Gr-1 mAb or IgG control at day 3 after ICH. C , Summarized results of modified Neurological Severity Score (mNSS) and corner-turning test scores in ICH mice receiving indicated treatments. D , Data points show brain water content in ICH mice receiving indicated treatments. n=8 mice per group. Data are presented as mean±SD.
Figure Legend Snippet: Gr-1 + myeloid cells contribute to the benefit of MCC950 after intracerebral hemorrhage (ICH). A , Schematic diagram illustrates drug administration and experimental design. C57BL/6 mice received anti–Gr-1 mAb 1 day before and 1 day after ICH surgery. ICH was induced by injection of autologous blood. Thereafter, these mice received daily injections of MCC950 (10 mg/kg, intraperitoneal [IP]) or an equal volume of vehicle for 3 consecutive days starting immediately after ICH induction. At day 3 after ICH, neurodeficits and brain water content were assessed. B , Data points show the counts of circulating Gr-1 + cells in mice before ICH induction, or mice receiving anti–Gr-1 mAb or IgG control at day 3 after ICH. C , Summarized results of modified Neurological Severity Score (mNSS) and corner-turning test scores in ICH mice receiving indicated treatments. D , Data points show brain water content in ICH mice receiving indicated treatments. n=8 mice per group. Data are presented as mean±SD.

Techniques Used: Mouse Assay, Injection, Modification

18) Product Images from "Oral Escherichia coli Colonization Factor Antigen I (CFA/I) Fimbriae Ameliorate Arthritis via IL-35, not IL-27"

Article Title: Oral Escherichia coli Colonization Factor Antigen I (CFA/I) Fimbriae Ameliorate Arthritis via IL-35, not IL-27

Journal: Journal of immunology (Baltimore, Md. : 1950)

doi: 10.4049/jimmunol.1302018

Oral CFA/I fimbriae stimulate IL-35 expression by Foxp3 + CD39 + CD4 + T cells. C57BL/6 mice were challenged with CII and treated with CFA/I fimbriae as described, and on day 40, flow cytometry analysis of CD39 + CD4 + T cells stained for EBI3 and p35 subunits
Figure Legend Snippet: Oral CFA/I fimbriae stimulate IL-35 expression by Foxp3 + CD39 + CD4 + T cells. C57BL/6 mice were challenged with CII and treated with CFA/I fimbriae as described, and on day 40, flow cytometry analysis of CD39 + CD4 + T cells stained for EBI3 and p35 subunits

Techniques Used: Expressing, Mouse Assay, Flow Cytometry, Cytometry, Staining

A , CII-specific CD4 + T cells cytokine responses in C57BL/6, EBI3 −/− and WSX-1 −/− mice upon termination of clinical studies; bars indicate differences within species tested; * p
Figure Legend Snippet: A , CII-specific CD4 + T cells cytokine responses in C57BL/6, EBI3 −/− and WSX-1 −/− mice upon termination of clinical studies; bars indicate differences within species tested; * p

Techniques Used: Mouse Assay

Oral CFA/I fimbriae after collagen II (CII) challenge protects against collagen-induced arthritis (CIA). CIA was induced in C57BL/6 mice (10 per group) on day 0 upon s.c. injection of emulsified chick CII. On day 14 post-CII challenge, mice were orally
Figure Legend Snippet: Oral CFA/I fimbriae after collagen II (CII) challenge protects against collagen-induced arthritis (CIA). CIA was induced in C57BL/6 mice (10 per group) on day 0 upon s.c. injection of emulsified chick CII. On day 14 post-CII challenge, mice were orally

Techniques Used: Mouse Assay, Injection

19) Product Images from "Oncolytic Adenovirus Expressing IL-23 and p35 Elicits IFN-?- and TNF-?-Co-Producing T Cell-Mediated Antitumor Immunity"

Article Title: Oncolytic Adenovirus Expressing IL-23 and p35 Elicits IFN-?- and TNF-?-Co-Producing T Cell-Mediated Antitumor Immunity

Journal: PLoS ONE

doi: 10.1371/journal.pone.0067512

Antitumor effect and survival rate of tumor-bearing mice. Antitumor effect (a and c) and survival rate (b and d) in mice given PBS (◊), RdB (□), RdB/IL23 or dE1/IL23/p35 (♦), RdB/IL12 (▪), or RdB/IL23/p35 (•). C57BL/6 tumor-bearing mice were treated with intratumoral injections of 5×10 9 VP/50 μL (a and b; initial tumor volume: 130-150 mm 3 ) or 1×10 10 VP/150 μL (c and d; initial tumor volume: 500 mm 3 ) of Ads on days 0, 2, and 4. Tumor volume was monitored and recorded every day until the end of the study. The arrow represents Ad inoculation. Values represent the mean ± SE (6∼8 animals per group). **, P
Figure Legend Snippet: Antitumor effect and survival rate of tumor-bearing mice. Antitumor effect (a and c) and survival rate (b and d) in mice given PBS (◊), RdB (□), RdB/IL23 or dE1/IL23/p35 (♦), RdB/IL12 (▪), or RdB/IL23/p35 (•). C57BL/6 tumor-bearing mice were treated with intratumoral injections of 5×10 9 VP/50 μL (a and b; initial tumor volume: 130-150 mm 3 ) or 1×10 10 VP/150 μL (c and d; initial tumor volume: 500 mm 3 ) of Ads on days 0, 2, and 4. Tumor volume was monitored and recorded every day until the end of the study. The arrow represents Ad inoculation. Values represent the mean ± SE (6∼8 animals per group). **, P

Techniques Used: Mouse Assay

In vivo CD4 + /CD8 + T cells or antitumor cytokines (IFN-γ and/or TNF-α depletion in RdB/IL23/p35-administrated mice. C57BL/6 tumor-bearing mice were treated with intratumoral injections of RdB/IL23/p35 and intravenous injection of antibodies [anti-CD4 or anti-CD8 antibodies (a) anti-IFN-γ and/or anti-TNF-αantibodies (b)] according to the protocol described in the Materials and Methods. The control group received isotype-matched antibody (IgG). Tumor growth was monitored and recorded every day. Values represent the mean ± SE (6∼8 animals per group). *, P
Figure Legend Snippet: In vivo CD4 + /CD8 + T cells or antitumor cytokines (IFN-γ and/or TNF-α depletion in RdB/IL23/p35-administrated mice. C57BL/6 tumor-bearing mice were treated with intratumoral injections of RdB/IL23/p35 and intravenous injection of antibodies [anti-CD4 or anti-CD8 antibodies (a) anti-IFN-γ and/or anti-TNF-αantibodies (b)] according to the protocol described in the Materials and Methods. The control group received isotype-matched antibody (IgG). Tumor growth was monitored and recorded every day. Values represent the mean ± SE (6∼8 animals per group). *, P

Techniques Used: In Vivo, Mouse Assay, Injection

20) Product Images from "The SMILE transcriptional corepressor inhibits cAMP response element–binding protein (CREB)–mediated transactivation of gluconeogenic genes"

Article Title: The SMILE transcriptional corepressor inhibits cAMP response element–binding protein (CREB)–mediated transactivation of gluconeogenic genes

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.RA118.002196

CREB/CRTC2-induced increase in blood glucose level is reduced by WT SMILE, but not by leucine zipper–mutated SMILE. A–E , recombinant Ad-GFP, Ad-S171A, Ad-SMILE (WT SMILE), and Ad-L5V (leucine zipper mutant SMILE) were delivered by tail vein injection into C57BL/6 mice ( n = 6). A , in vivo experiment scheme. B , expression of G6Pase, Pepck, Pgc-1α, albumin, and SMILE was analyzed by real-time quantitative RT-PCR. Expression was normalized to that of ribosomal L32. C , fasting glucose measured after 16 h of fasting, n = 6. D , glucose tolerance test in mice, glucose (2g/kg), right panel area under curve ( AUC ), n = 6. E , pyruvate tolerance test in mice, pyruvate (2g/kg), right panel , AUC, n = 6. F , insulin tolerance test (0.75 units/kg) in mice, right panel AUC, n = 6. Statistical test for B–E : One-way ANOVA with Tukey's multiple comparison test. n.s. , not significant; **, p
Figure Legend Snippet: CREB/CRTC2-induced increase in blood glucose level is reduced by WT SMILE, but not by leucine zipper–mutated SMILE. A–E , recombinant Ad-GFP, Ad-S171A, Ad-SMILE (WT SMILE), and Ad-L5V (leucine zipper mutant SMILE) were delivered by tail vein injection into C57BL/6 mice ( n = 6). A , in vivo experiment scheme. B , expression of G6Pase, Pepck, Pgc-1α, albumin, and SMILE was analyzed by real-time quantitative RT-PCR. Expression was normalized to that of ribosomal L32. C , fasting glucose measured after 16 h of fasting, n = 6. D , glucose tolerance test in mice, glucose (2g/kg), right panel area under curve ( AUC ), n = 6. E , pyruvate tolerance test in mice, pyruvate (2g/kg), right panel , AUC, n = 6. F , insulin tolerance test (0.75 units/kg) in mice, right panel AUC, n = 6. Statistical test for B–E : One-way ANOVA with Tukey's multiple comparison test. n.s. , not significant; **, p

Techniques Used: Recombinant, Mutagenesis, Injection, Mouse Assay, In Vivo, Expressing, Pyrolysis Gas Chromatography, Quantitative RT-PCR

21) Product Images from "KEAP1-modifying small molecule reveals muted NRF2 signaling responses in neural stem cells from Huntington's disease patients"

Article Title: KEAP1-modifying small molecule reveals muted NRF2 signaling responses in neural stem cells from Huntington's disease patients

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.1614943114

MIND4 pharmacokinetics in serum (filled circles) were examined by HPLC analysis in WT male C57BL/6 mice ( n = 3) at 0.5, 1.0, 2, 4, 8, and 24 h at compound treatment dose of 50 mg/kg; blood C max = 477 ng/mL at 0.5-h time point. Brain levels (red triangle) were analyzed in cortices from mice ( n = 3) killed at 0.5 h after i.p. injection; C brain = 120 ng/mL and respective brain/serum ratio, 0.25.
Figure Legend Snippet: MIND4 pharmacokinetics in serum (filled circles) were examined by HPLC analysis in WT male C57BL/6 mice ( n = 3) at 0.5, 1.0, 2, 4, 8, and 24 h at compound treatment dose of 50 mg/kg; blood C max = 477 ng/mL at 0.5-h time point. Brain levels (red triangle) were analyzed in cortices from mice ( n = 3) killed at 0.5 h after i.p. injection; C brain = 120 ng/mL and respective brain/serum ratio, 0.25.

Techniques Used: High Performance Liquid Chromatography, Mouse Assay, Injection

22) Product Images from "Exposure to a social stressor induces translocation of commensal lactobacilli to the spleen and priming of the innate immune system"

Article Title: Exposure to a social stressor induces translocation of commensal lactobacilli to the spleen and priming of the innate immune system

Journal: Journal of immunology (Baltimore, Md. : 1950)

doi: 10.4049/jimmunol.1601269

In vitro stimulation with heat-killed Lactobacillus animalis induces pro-inflammatory cytokine mRNA in monocytes and neutrophils Spleen monocytes (A) and neutrophils (B) were isolated by negative selection from non-stressed C57BL/6 mice and stimulated with heat-killed Lactobacillus animalis at 2.5 and 5 bacteria/cell for 6 hrs. Cytokine mRNA levels were analyzed by qRT-PCR and the relative mRNA expression was determined using β-actin mRNA as the normalizer. Data represents means ±SEM of 3–7 replicate wells from two experiments. Statistical analysis was performed by Student t test with Welch’s correction for unequal variance *p
Figure Legend Snippet: In vitro stimulation with heat-killed Lactobacillus animalis induces pro-inflammatory cytokine mRNA in monocytes and neutrophils Spleen monocytes (A) and neutrophils (B) were isolated by negative selection from non-stressed C57BL/6 mice and stimulated with heat-killed Lactobacillus animalis at 2.5 and 5 bacteria/cell for 6 hrs. Cytokine mRNA levels were analyzed by qRT-PCR and the relative mRNA expression was determined using β-actin mRNA as the normalizer. Data represents means ±SEM of 3–7 replicate wells from two experiments. Statistical analysis was performed by Student t test with Welch’s correction for unequal variance *p

Techniques Used: In Vitro, Isolation, Selection, Mouse Assay, Quantitative RT-PCR, Expressing

23) Product Images from "Unraveling the Novel Protective Effect of Patchouli Alcohol Against Helicobacter pylori-Induced Gastritis: Insights Into the Molecular Mechanism in vitro and in vivo"

Article Title: Unraveling the Novel Protective Effect of Patchouli Alcohol Against Helicobacter pylori-Induced Gastritis: Insights Into the Molecular Mechanism in vitro and in vivo

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2018.01347

Effects of PA on gastric oxidative factor levels and pro-inflammatory cytokine expression in Helicobacter pylori -infected mice. C57BL/6 mice were infected with H. pylori SS1 strain for 10 weeks and treated with vehicle (model group), triple therapy (omeprazole + MET +CLR, positive group), and different doses of PA (PA 5, 10, and 20 mg/kg groups) for 2 weeks. The control group received vehicle treatment for 2 weeks without H. pylori challenge. After the treatment, the gastric antrum and pylorus were removed, homogenized and centrifuged to obtain the supernatant. The supernatant was quantified by BCA assay and stored at −80°C for further detection. (A) Oxidant factor levels (CAT, MPO, and SOD activities; NP-SH and MDA contents; GSH/GSSG). (B) Pro-inflammatory cytokine expression levels (IL-1β, KC, and IL-6). Δ P
Figure Legend Snippet: Effects of PA on gastric oxidative factor levels and pro-inflammatory cytokine expression in Helicobacter pylori -infected mice. C57BL/6 mice were infected with H. pylori SS1 strain for 10 weeks and treated with vehicle (model group), triple therapy (omeprazole + MET +CLR, positive group), and different doses of PA (PA 5, 10, and 20 mg/kg groups) for 2 weeks. The control group received vehicle treatment for 2 weeks without H. pylori challenge. After the treatment, the gastric antrum and pylorus were removed, homogenized and centrifuged to obtain the supernatant. The supernatant was quantified by BCA assay and stored at −80°C for further detection. (A) Oxidant factor levels (CAT, MPO, and SOD activities; NP-SH and MDA contents; GSH/GSSG). (B) Pro-inflammatory cytokine expression levels (IL-1β, KC, and IL-6). Δ P

Techniques Used: Expressing, Infection, Mouse Assay, BIA-KA, Multiple Displacement Amplification

24) Product Images from "Synthetic amyloid-\u03b2 oligomers impair long-term memory independently of cellular prion protein"

Article Title: Synthetic amyloid-\u03b2 oligomers impair long-term memory independently of cellular prion protein

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.0911829107

Aβ 1–42 oligomers impair recognition memory in mice. ( A ) Effect of Aβ initial state, oligomers, and fibrils on memory was investigated in C57BL/6 male mice in the object recognition task after two i.c.v. injections (7.5 μL;
Figure Legend Snippet: Aβ 1–42 oligomers impair recognition memory in mice. ( A ) Effect of Aβ initial state, oligomers, and fibrils on memory was investigated in C57BL/6 male mice in the object recognition task after two i.c.v. injections (7.5 μL;

Techniques Used: Mouse Assay

25) Product Images from "Inhibition of ERK1/2 Pathway Suppresses Adiponectin Secretion via Accelerating Protein Degradation by Ubiquitin-Proteasome System: Relevance to Obesity-related Adiponectin Decline"

Article Title: Inhibition of ERK1/2 Pathway Suppresses Adiponectin Secretion via Accelerating Protein Degradation by Ubiquitin-Proteasome System: Relevance to Obesity-related Adiponectin Decline

Journal: Metabolism: clinical and experimental

doi: 10.1016/j.metabol.2013.01.025

Obesity-related adiponectin decline is associated with suppressed ERK1/2 and AKT activation in adipose tissue. Male C57BL/6 mice were exposed to high-fat diet for eight weeks. (A) body weight; (B) epididymal fat mass; (C) adiponectin and PPAR-γ
Figure Legend Snippet: Obesity-related adiponectin decline is associated with suppressed ERK1/2 and AKT activation in adipose tissue. Male C57BL/6 mice were exposed to high-fat diet for eight weeks. (A) body weight; (B) epididymal fat mass; (C) adiponectin and PPAR-γ

Techniques Used: Activation Assay, Mouse Assay

26) Product Images from "Ethanol elevates physiological all-trans-retinoic acid levels in select loci through altering retinoid metabolism in multiple loci: a potential mechanism of ethanol toxicity"

Article Title: Ethanol elevates physiological all-trans-retinoic acid levels in select loci through altering retinoid metabolism in multiple loci: a potential mechanism of ethanol toxicity

Journal: The FASEB Journal

doi: 10.1096/fj.09-141572

LC-MS/MS quantification of ethanol effects on endogenous atRA. A ) atRA in tissues and serum after an acute i.p. dose of 3.5 g/kg ethanol to 3-mo-old male C57BL/6 mice ( n =4–8). Inset: corresponding BAC% values. B , C ) Increases in e19 fetal hippocampus ( B ) and cortex atRA ( C ) in fetal mice from 2- to 3-mo-old dams after dam ingestion of 6.5% ethanol from e13 to e19. Each set of bars represents a pool of fetal hippocampi/cortexes ( n =4–10) from an individual litter and the corresponding dam BAC%. D , E ) atRA in tissues and serum ( D ) or brain regions ( E ) of 3-mo-old male mice after 1-mo feeding of control or 6.5% ethanol diets ( n =4–8). Hip, hippocampus; Cx, cortex; OB, olfactory bulb; Th, thalamus; Cb, cerebellum; St, striatum. F, G ) Representative LC-MS/MS chromatograms for liver ( F ) and hippocampus ( G ), showing the effect of ethanol. H ) MS/MS spectrum of RA, showing its characteristic fragmentation pattern: * P
Figure Legend Snippet: LC-MS/MS quantification of ethanol effects on endogenous atRA. A ) atRA in tissues and serum after an acute i.p. dose of 3.5 g/kg ethanol to 3-mo-old male C57BL/6 mice ( n =4–8). Inset: corresponding BAC% values. B , C ) Increases in e19 fetal hippocampus ( B ) and cortex atRA ( C ) in fetal mice from 2- to 3-mo-old dams after dam ingestion of 6.5% ethanol from e13 to e19. Each set of bars represents a pool of fetal hippocampi/cortexes ( n =4–10) from an individual litter and the corresponding dam BAC%. D , E ) atRA in tissues and serum ( D ) or brain regions ( E ) of 3-mo-old male mice after 1-mo feeding of control or 6.5% ethanol diets ( n =4–8). Hip, hippocampus; Cx, cortex; OB, olfactory bulb; Th, thalamus; Cb, cerebellum; St, striatum. F, G ) Representative LC-MS/MS chromatograms for liver ( F ) and hippocampus ( G ), showing the effect of ethanol. H ) MS/MS spectrum of RA, showing its characteristic fragmentation pattern: * P

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Mouse Assay, BAC Assay

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Charles River Laboratories male c57bl 6 mice
    Betaine supplementation alleviates plasma adiponectin reduction and 4-HNE increase in adipose tissue in HF diet mice. Male <t>C57BL/6</t> mice were fed HF diets with or without betaine [1% (wt/vol)] supplementation in the drinking water for 12 weeks. At last,
    Male C57bl 6 Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/male c57bl 6 mice/product/Charles River Laboratories
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    male c57bl 6 mice - by Bioz Stars, 2022-09
    90/100 stars
      Buy from Supplier

    88
    Charles River Laboratories female wt c57bl 6 mice
    Subcellular localization of δOR immunoreactivity in the dorsal horn of the spinal cord of untreated versus morphine-pretreated wild-type mice. <t>C57BL/6</t> WT mice were pretreated ( D–F ) or not ( A–C ) with morphine as described and were
    Female Wt C57bl 6 Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/female wt c57bl 6 mice/product/Charles River Laboratories
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    female wt c57bl 6 mice - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

    88
    Charles River Laboratories male c57bl 6 wt mice
    No difference in liver damage after HS/BFF in IL18ko, IL18Rko or IL1R1ko mice compared with WT <t>(C57BL/6).</t> AST and ALT levels in plasma at 6 h after HS/BFF are shown. n = 3 or 6/group. Data show mean ± SEM.
    Male C57bl 6 Wt Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/male c57bl 6 wt mice/product/Charles River Laboratories
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    male c57bl 6 wt mice - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

    90
    Charles River Laboratories c57bl 6 mice
    Effect of control solution, NLC and NLC-R11 containing SP and KP treatment on the response of ACD model in <t>C57BL/6</t> mice. Data represent mean ± SD, n=6; significance solution against NLC-R11, *p
    C57bl 6 Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c57bl 6 mice/product/Charles River Laboratories
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c57bl 6 mice - by Bioz Stars, 2022-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Betaine supplementation alleviates plasma adiponectin reduction and 4-HNE increase in adipose tissue in HF diet mice. Male C57BL/6 mice were fed HF diets with or without betaine [1% (wt/vol)] supplementation in the drinking water for 12 weeks. At last,

    Journal: Molecular and cellular endocrinology

    Article Title: 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPAR\u03b3 and accelerating ubiquitin\u2013proteasome degradation

    doi: 10.1016/j.mce.2011.10.027

    Figure Lengend Snippet: Betaine supplementation alleviates plasma adiponectin reduction and 4-HNE increase in adipose tissue in HF diet mice. Male C57BL/6 mice were fed HF diets with or without betaine [1% (wt/vol)] supplementation in the drinking water for 12 weeks. At last,

    Article Snippet: Male C57BL/6 mice were fed with either Control diet (Con) or high-fat (HF) diet for 12 weeks.

    Techniques: Mouse Assay

    Long-term high-fat diet feeding caused adiposity and decreased plasma adiponectin level, which is associated with increased oxidative stress in adipose tissue. Male C57BL/6 mice were fed with control and high-fat (HF) diets. Twelve weeks later, the epididymal

    Journal: Molecular and cellular endocrinology

    Article Title: 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPAR\u03b3 and accelerating ubiquitin\u2013proteasome degradation

    doi: 10.1016/j.mce.2011.10.027

    Figure Lengend Snippet: Long-term high-fat diet feeding caused adiposity and decreased plasma adiponectin level, which is associated with increased oxidative stress in adipose tissue. Male C57BL/6 mice were fed with control and high-fat (HF) diets. Twelve weeks later, the epididymal

    Article Snippet: Male C57BL/6 mice were fed with either Control diet (Con) or high-fat (HF) diet for 12 weeks.

    Techniques: Mouse Assay

    Subcellular localization of δOR immunoreactivity in the dorsal horn of the spinal cord of untreated versus morphine-pretreated wild-type mice. C57BL/6 WT mice were pretreated ( D–F ) or not ( A–C ) with morphine as described and were

    Journal: The Journal of Neuroscience

    Article Title: Regulation of δ-Opioid Receptor Trafficking via μ-Opioid Receptor Stimulation: Evidence from μ-Opioid Receptor Knock-Out Mice

    doi: 10.1523/JNEUROSCI.23-12-04888.2003

    Figure Lengend Snippet: Subcellular localization of δOR immunoreactivity in the dorsal horn of the spinal cord of untreated versus morphine-pretreated wild-type mice. C57BL/6 WT mice were pretreated ( D–F ) or not ( A–C ) with morphine as described and were

    Article Snippet: Behavioral experiments used both male and female WT C57BL/6 mice (8–15 weeks of age; Charles River, Québec, Canada).

    Techniques: Mouse Assay

    Immunogold electron microscopic localization of δOR in the dorsal horn of the spinal cord of untreated and morphine-pretreated μOR knock-out mice. C57BL/6 KO mice were pretreated ( D–F ) or not ( A–C ) with morphine as described

    Journal: The Journal of Neuroscience

    Article Title: Regulation of δ-Opioid Receptor Trafficking via μ-Opioid Receptor Stimulation: Evidence from μ-Opioid Receptor Knock-Out Mice

    doi: 10.1523/JNEUROSCI.23-12-04888.2003

    Figure Lengend Snippet: Immunogold electron microscopic localization of δOR in the dorsal horn of the spinal cord of untreated and morphine-pretreated μOR knock-out mice. C57BL/6 KO mice were pretreated ( D–F ) or not ( A–C ) with morphine as described

    Article Snippet: Behavioral experiments used both male and female WT C57BL/6 mice (8–15 weeks of age; Charles River, Québec, Canada).

    Techniques: Knock-Out, Mouse Assay

    Prolonged treatment with morphine leads to enhanced δOR-mediated antinociception in C57BL/6 mice. WT male and female mice were pretreated ( B ) or not ( A ) with morphine sulfate at 5, 8, 10, 15 mg/kg (subcutaneously every 12 hr). At 12 hr after the

    Journal: The Journal of Neuroscience

    Article Title: Regulation of δ-Opioid Receptor Trafficking via μ-Opioid Receptor Stimulation: Evidence from μ-Opioid Receptor Knock-Out Mice

    doi: 10.1523/JNEUROSCI.23-12-04888.2003

    Figure Lengend Snippet: Prolonged treatment with morphine leads to enhanced δOR-mediated antinociception in C57BL/6 mice. WT male and female mice were pretreated ( B ) or not ( A ) with morphine sulfate at 5, 8, 10, 15 mg/kg (subcutaneously every 12 hr). At 12 hr after the

    Article Snippet: Behavioral experiments used both male and female WT C57BL/6 mice (8–15 weeks of age; Charles River, Québec, Canada).

    Techniques: Mouse Assay

    No difference in liver damage after HS/BFF in IL18ko, IL18Rko or IL1R1ko mice compared with WT (C57BL/6). AST and ALT levels in plasma at 6 h after HS/BFF are shown. n = 3 or 6/group. Data show mean ± SEM.

    Journal: Molecular Medicine

    Article Title: Caspase-1 Is Hepatoprotective during Trauma and Hemorrhagic Shock by Reducing Liver Injury and Inflammation

    doi: 10.2119/molmed.2011.00015

    Figure Lengend Snippet: No difference in liver damage after HS/BFF in IL18ko, IL18Rko or IL1R1ko mice compared with WT (C57BL/6). AST and ALT levels in plasma at 6 h after HS/BFF are shown. n = 3 or 6/group. Data show mean ± SEM.

    Article Snippet: Male C57BL/6 (WT) mice (Charles River Laboratories International, Wilmington, MA, USA), caspase-1−/− mice (a gift from Richard Flavell, Yale University, New Haven, CT, USA [ ]), and NLRP3 (NALP3, pyrin-1)−/− mice (Millennium Pharmaceuticals, Boston, MA, USA) aged 7–11 weeks, weighing 21–30 g, were used in experiments.

    Techniques: Mouse Assay, AST Assay

    Caspase-1 is activated after HS/BFF. (A) Western blot images showing caspase-1 is activated (cleaved) in liver of WT (C57BL/6) mice at 6 h after HS/BFF. (B) Caspase-1 −/− mice did not express IL18 at baseline or after HS/BFF, but NLRP3 −/−

    Journal: Molecular Medicine

    Article Title: Caspase-1 Is Hepatoprotective during Trauma and Hemorrhagic Shock by Reducing Liver Injury and Inflammation

    doi: 10.2119/molmed.2011.00015

    Figure Lengend Snippet: Caspase-1 is activated after HS/BFF. (A) Western blot images showing caspase-1 is activated (cleaved) in liver of WT (C57BL/6) mice at 6 h after HS/BFF. (B) Caspase-1 −/− mice did not express IL18 at baseline or after HS/BFF, but NLRP3 −/−

    Article Snippet: Male C57BL/6 (WT) mice (Charles River Laboratories International, Wilmington, MA, USA), caspase-1−/− mice (a gift from Richard Flavell, Yale University, New Haven, CT, USA [ ]), and NLRP3 (NALP3, pyrin-1)−/− mice (Millennium Pharmaceuticals, Boston, MA, USA) aged 7–11 weeks, weighing 21–30 g, were used in experiments.

    Techniques: Western Blot, Mouse Assay

    Effect of control solution, NLC and NLC-R11 containing SP and KP treatment on the response of ACD model in C57BL/6 mice. Data represent mean ± SD, n=6; significance solution against NLC-R11, *p

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Enhanced skin permeation using polyarginine modified nanostructured lipid carriers

    doi: 10.1016/j.jconrel.2012.05.011

    Figure Lengend Snippet: Effect of control solution, NLC and NLC-R11 containing SP and KP treatment on the response of ACD model in C57BL/6 mice. Data represent mean ± SD, n=6; significance solution against NLC-R11, *p

    Article Snippet: Hairless rats (CD ® (SD) HrBi, Male) and C57BL/6 mice (6 weeks old, male) (Charles River Laboratories, Wilmington, MA) were grouped and housed (n = 6 per cage) in cages with bedding.

    Techniques: Mouse Assay