maldi ltq orbitrap mass spectrometer  (Thermo Fisher)


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    Name:
    MALDI LTQ Orbitrap Spectrometer
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    Thermo Fisher maldi ltq orbitrap mass spectrometer
    Workflow for MS imaging of Pseudonocardia on the ant propleural plate. a) Photograph and b) cartoon of Pseudonocardia on the ant exoskeleton. c) Grooves were cut into glass slides and double-sided tape was applied to the back of the slide. The ant thoraxes were removed and positioned into the groove of the slide with the propleural plate facing outward and even with the top of the slide. An additional thin strip of double-sided tape was applied below the propleural plate to stabilize and secure the thorax into place. d) Matrix was applied to the slide using an automatic sprayer. e) A laser was fired at the sample to ionize compounds of interest and introduce them into the mass spectrometer. f) An array of mass spectra was acquired using a <t>MALDI-LTQ</t> <t>Orbitrap</t> and g) compiled into MS images. Photo courtesy of Alex Wild.

    https://www.bioz.com/result/maldi ltq orbitrap mass spectrometer/product/Thermo Fisher
    Average 96 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    maldi ltq orbitrap mass spectrometer - by Bioz Stars, 2020-05
    96/100 stars

    Images

    1) Product Images from "Imaging with Mass Spectrometry of Bacteria on the Exoskeleton of Fungus-Growing Ants"

    Article Title: Imaging with Mass Spectrometry of Bacteria on the Exoskeleton of Fungus-Growing Ants

    Journal: ACS chemical biology

    doi: 10.1021/acschembio.7b00038

    Workflow for MS imaging of Pseudonocardia on the ant propleural plate. a) Photograph and b) cartoon of Pseudonocardia on the ant exoskeleton. c) Grooves were cut into glass slides and double-sided tape was applied to the back of the slide. The ant thoraxes were removed and positioned into the groove of the slide with the propleural plate facing outward and even with the top of the slide. An additional thin strip of double-sided tape was applied below the propleural plate to stabilize and secure the thorax into place. d) Matrix was applied to the slide using an automatic sprayer. e) A laser was fired at the sample to ionize compounds of interest and introduce them into the mass spectrometer. f) An array of mass spectra was acquired using a MALDI-LTQ Orbitrap and g) compiled into MS images. Photo courtesy of Alex Wild.
    Figure Legend Snippet: Workflow for MS imaging of Pseudonocardia on the ant propleural plate. a) Photograph and b) cartoon of Pseudonocardia on the ant exoskeleton. c) Grooves were cut into glass slides and double-sided tape was applied to the back of the slide. The ant thoraxes were removed and positioned into the groove of the slide with the propleural plate facing outward and even with the top of the slide. An additional thin strip of double-sided tape was applied below the propleural plate to stabilize and secure the thorax into place. d) Matrix was applied to the slide using an automatic sprayer. e) A laser was fired at the sample to ionize compounds of interest and introduce them into the mass spectrometer. f) An array of mass spectra was acquired using a MALDI-LTQ Orbitrap and g) compiled into MS images. Photo courtesy of Alex Wild.

    Techniques Used: Mass Spectrometry, Imaging, Stripping Membranes, Introduce

    Related Articles

    other:

    Article Title: Visualizing Neurotransmitters and Metabolites in the Central Nervous System by High Resolution and High Accuracy Mass Spectrometric Imaging
    Article Snippet: The MALDI LTQ Orbitrap XL instrument (Thermo Scientific, Bremen, Germany) equipped with a commercial 60 Hz N2 laser at 337 nm (Lasertechnik Berlin GmbH, Berlin, Germany) was used for MSI.

    Imaging:

    Article Title: Localization of tamoxifen in human breast cancer tumors by MALDI mass spectrometry imaging
    Article Snippet: .. MALDI mass spectrometry imaging analysis The MALDI-MS imaging acquisition was performed using a MALDI LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) with a method that employed 10 laser shots at 10 µJ. ..

    Article Title: Investigation of signaling molecules and metabolites found in crustacean hemolymph via in vivo microdialysis using a multi-faceted mass spectrometric platform
    Article Snippet: .. The matrix covered CE trace was then analyzed using a MALDI LTQ Orbitrap XL (Thermo Scientific, San Jose, CA, USA) with imaging experimental set up. ..

    Mass Spectrometry:

    Article Title: Localization of tamoxifen in human breast cancer tumors by MALDI mass spectrometry imaging
    Article Snippet: .. MALDI mass spectrometry imaging analysis The MALDI-MS imaging acquisition was performed using a MALDI LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) with a method that employed 10 laser shots at 10 µJ. ..

    Article Title: Imaging with Mass Spectrometry of Bacteria on the Exoskeleton of Fungus-Growing Ants
    Article Snippet: .. A MALDI- LTQ Orbitrap mass spectrometer (Thermo Scientific) equipped with an N2 laser (spot diameter of 75 μ m) was used in positive ion mode for MSI. ..

    Article Title: Resolving the Composition of Protein Complexes using a MALDI LTQ Orbitrap
    Article Snippet: .. Mass spectrometric analyses were performed on a MALDI LTQ Orbitrap XL mass spectrometer (Thermo Electron, Bremen, Germany). .. The MALDI Duo source configuration on the LTQ Orbitrap has been modified from the previous model on the vMALDI LTQ instrument, which incorporated a nitrogen laser with 20 Hz firing rate, coupled to the LTQ mass spectrometer via a fiber optic cable.

    Article Title: Localization of tamoxifen in human breast cancer tumors by MALDI mass spectrometry imaging
    Article Snippet: .. Following initial dissolving of a pill, the entire content was used for determining the ionization properties of tamoxifen on a MALDI LTQ Orbitrap XL mass spectrometer. ..

    Article Title: A Targeted Spatial-Temporal Proteomics Approach Implicates Multiple Cellular Trafficking Pathways in Human Cytomegalovirus Virion Maturation *
    Article Snippet: .. Mass spectrometric analyses were performed on a MALDI LTQ Orbitrap XL mass spectrometer (Thermo Electron, Bremen, Germany) ( ) as described ( ). .. Instrument calibration was performed by external calibration using standard peptide mixtures for normal and high mass ranges (ProteoMassTM calibration kit, Sigma) as described ( ).

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  • 99
    Thermo Fisher maldi linear ion trap orbitrap hybrid mass spectrometer
    Accumulation of lipid droplets and spatial distribution of different triacylglycerol (TAG) molecular species within transgenic leaf tissue. (a) 3D reconstructed z-stacks of confocal images from wild-type (left) and transgenic (right) leaf tissue. Neutral lipids including TAG, stained with BODIPY, appear as green droplets within the leaf mesophyll cells that contain visible chloroplasts (red autofluorescence). Note that non-TAG features also fluorescing green include the vascular bundle and leaf epidermis. (b) Bright-field microscopy images of wild-type (upper) and transgenic (lower) leaf cross-sections subsequently used for <t>MALDI-Orbitrap</t> imaging. Scale bars correspond to 1000 microns. (c) Ratio of TAG total ion counts (TIC; all TAG molecular species summed at each analysed position) to PC-TIC as detected by MALDI-Orbitrap imaging in wild-type (upper) and transgenic (lower) leaf cross-sections. (d) Spatial distribution of selected dominant TAG and PC species across a transgenic leaf cross-section as observed by MALDI-Orbitrap.
    Maldi Linear Ion Trap Orbitrap Hybrid Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maldi linear ion trap orbitrap hybrid mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    maldi linear ion trap orbitrap hybrid mass spectrometer - by Bioz Stars, 2020-05
    99/100 stars
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    92
    Thermo Fisher maldi ltq orbitrap xl mass spectrometer
    Full scan spectra of protein mixtures: <t>MALDI-TOF</t> spectrum of histone mixture, 10 mg/mL (a), HRAM <t>MAI-Orbitrap</t> spectrum of histone mixture, 1 mg/mL (b) and HRAM MAI-Orbitrap spectrum of rat brain protein extract.
    Maldi Ltq Orbitrap Xl Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maldi ltq orbitrap xl mass spectrometer/product/Thermo Fisher
    Average 92 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    maldi ltq orbitrap xl mass spectrometer - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    96
    Thermo Fisher maldi ltq orbitrap mass spectrometer
    Workflow for MS imaging of Pseudonocardia on the ant propleural plate. a) Photograph and b) cartoon of Pseudonocardia on the ant exoskeleton. c) Grooves were cut into glass slides and double-sided tape was applied to the back of the slide. The ant thoraxes were removed and positioned into the groove of the slide with the propleural plate facing outward and even with the top of the slide. An additional thin strip of double-sided tape was applied below the propleural plate to stabilize and secure the thorax into place. d) Matrix was applied to the slide using an automatic sprayer. e) A laser was fired at the sample to ionize compounds of interest and introduce them into the mass spectrometer. f) An array of mass spectra was acquired using a <t>MALDI-LTQ</t> <t>Orbitrap</t> and g) compiled into MS images. Photo courtesy of Alex Wild.
    Maldi Ltq Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maldi ltq orbitrap mass spectrometer/product/Thermo Fisher
    Average 96 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    maldi ltq orbitrap mass spectrometer - by Bioz Stars, 2020-05
    96/100 stars
      Buy from Supplier

    Image Search Results


    Accumulation of lipid droplets and spatial distribution of different triacylglycerol (TAG) molecular species within transgenic leaf tissue. (a) 3D reconstructed z-stacks of confocal images from wild-type (left) and transgenic (right) leaf tissue. Neutral lipids including TAG, stained with BODIPY, appear as green droplets within the leaf mesophyll cells that contain visible chloroplasts (red autofluorescence). Note that non-TAG features also fluorescing green include the vascular bundle and leaf epidermis. (b) Bright-field microscopy images of wild-type (upper) and transgenic (lower) leaf cross-sections subsequently used for MALDI-Orbitrap imaging. Scale bars correspond to 1000 microns. (c) Ratio of TAG total ion counts (TIC; all TAG molecular species summed at each analysed position) to PC-TIC as detected by MALDI-Orbitrap imaging in wild-type (upper) and transgenic (lower) leaf cross-sections. (d) Spatial distribution of selected dominant TAG and PC species across a transgenic leaf cross-section as observed by MALDI-Orbitrap.

    Journal: Plant Biotechnology Journal

    Article Title: Metabolic engineering of biomass for high energy density: oilseed-like triacylglycerol yields from plant leaves

    doi: 10.1111/pbi.12131

    Figure Lengend Snippet: Accumulation of lipid droplets and spatial distribution of different triacylglycerol (TAG) molecular species within transgenic leaf tissue. (a) 3D reconstructed z-stacks of confocal images from wild-type (left) and transgenic (right) leaf tissue. Neutral lipids including TAG, stained with BODIPY, appear as green droplets within the leaf mesophyll cells that contain visible chloroplasts (red autofluorescence). Note that non-TAG features also fluorescing green include the vascular bundle and leaf epidermis. (b) Bright-field microscopy images of wild-type (upper) and transgenic (lower) leaf cross-sections subsequently used for MALDI-Orbitrap imaging. Scale bars correspond to 1000 microns. (c) Ratio of TAG total ion counts (TIC; all TAG molecular species summed at each analysed position) to PC-TIC as detected by MALDI-Orbitrap imaging in wild-type (upper) and transgenic (lower) leaf cross-sections. (d) Spatial distribution of selected dominant TAG and PC species across a transgenic leaf cross-section as observed by MALDI-Orbitrap.

    Article Snippet: Sections were scanned at 25 micron step-size in a MALDI linear ion-trap-Orbitrap hybrid mass spectrometer (MALDI LTQ Orbitrap-XL; Thermo Fisher Scientific, Bremen, Germany).

    Techniques: Transgenic Assay, Staining, Microscopy, Imaging

    Full scan spectra of protein mixtures: MALDI-TOF spectrum of histone mixture, 10 mg/mL (a), HRAM MAI-Orbitrap spectrum of histone mixture, 1 mg/mL (b) and HRAM MAI-Orbitrap spectrum of rat brain protein extract.

    Journal: Analytical and bioanalytical chemistry

    Article Title: Coupling Matrix-Assisted Ionization with High Resolution Mass Spectrometry and Electron Transfer Dissociation to Characterize Intact Proteins and Post-Translational Modifications

    doi: 10.1007/s00216-017-0611-4

    Figure Lengend Snippet: Full scan spectra of protein mixtures: MALDI-TOF spectrum of histone mixture, 10 mg/mL (a), HRAM MAI-Orbitrap spectrum of histone mixture, 1 mg/mL (b) and HRAM MAI-Orbitrap spectrum of rat brain protein extract.

    Article Snippet: In the comparison study, the MALDI and MAI (MALDI) spectra were acquired on MALDI-LTQ-Orbitrap XL mass spectrometer (Thermo Scientific, Bremen, Germany), which is also a hybrid ion-trap-Orbitrap mass spectrometer but with MALDI source.

    Techniques:

    Workflow for MS imaging of Pseudonocardia on the ant propleural plate. a) Photograph and b) cartoon of Pseudonocardia on the ant exoskeleton. c) Grooves were cut into glass slides and double-sided tape was applied to the back of the slide. The ant thoraxes were removed and positioned into the groove of the slide with the propleural plate facing outward and even with the top of the slide. An additional thin strip of double-sided tape was applied below the propleural plate to stabilize and secure the thorax into place. d) Matrix was applied to the slide using an automatic sprayer. e) A laser was fired at the sample to ionize compounds of interest and introduce them into the mass spectrometer. f) An array of mass spectra was acquired using a MALDI-LTQ Orbitrap and g) compiled into MS images. Photo courtesy of Alex Wild.

    Journal: ACS chemical biology

    Article Title: Imaging with Mass Spectrometry of Bacteria on the Exoskeleton of Fungus-Growing Ants

    doi: 10.1021/acschembio.7b00038

    Figure Lengend Snippet: Workflow for MS imaging of Pseudonocardia on the ant propleural plate. a) Photograph and b) cartoon of Pseudonocardia on the ant exoskeleton. c) Grooves were cut into glass slides and double-sided tape was applied to the back of the slide. The ant thoraxes were removed and positioned into the groove of the slide with the propleural plate facing outward and even with the top of the slide. An additional thin strip of double-sided tape was applied below the propleural plate to stabilize and secure the thorax into place. d) Matrix was applied to the slide using an automatic sprayer. e) A laser was fired at the sample to ionize compounds of interest and introduce them into the mass spectrometer. f) An array of mass spectra was acquired using a MALDI-LTQ Orbitrap and g) compiled into MS images. Photo courtesy of Alex Wild.

    Article Snippet: A MALDI- LTQ Orbitrap mass spectrometer (Thermo Scientific) equipped with an N2 laser (spot diameter of 75 μ m) was used in positive ion mode for MSI.

    Techniques: Mass Spectrometry, Imaging, Stripping Membranes, Introduce

    HRMSI spectra averaged from a blue crab brain section acquired on a MALDI LTQ Orbitrap XL. (a) Averaged HRMS spectra displaying the m / z range of 90–500. (b–d) Zoomed-in spectra shown in (a) over the range of (b) m / z 147.06–147.12, (c) m / z 175.98–176.04, and (d) m / z 385.94–386.14. Panels (e) and (f) display different distributions of two ions present in (c) from blue crab brain.

    Journal: ACS Chemical Neuroscience

    Article Title: Visualizing Neurotransmitters and Metabolites in the Central Nervous System by High Resolution and High Accuracy Mass Spectrometric Imaging

    doi: 10.1021/cn400065k

    Figure Lengend Snippet: HRMSI spectra averaged from a blue crab brain section acquired on a MALDI LTQ Orbitrap XL. (a) Averaged HRMS spectra displaying the m / z range of 90–500. (b–d) Zoomed-in spectra shown in (a) over the range of (b) m / z 147.06–147.12, (c) m / z 175.98–176.04, and (d) m / z 385.94–386.14. Panels (e) and (f) display different distributions of two ions present in (c) from blue crab brain.

    Article Snippet: The regional MS spectra and MS images obtained on the MALDI LTQ Orbitrap XL were processed using ImageQuest (Thermo Scientific).

    Techniques:

    Comparison between HRMS- and MRMS-produced spectra acquired from serial rat CNS sections. (a) HRMS-generated spectrum corresponding to the region of interest (ROI) localized in the cortex region of a rat brain section as highlighted in (b). The inset shows an enlarged m / z range of 362–366 obtained on a MALDI LTQ Orbitrap XL. (c) MRMS-generated mass spectrum corresponding to an identical ROI from the cortex region of a serial rat brain section as shown in (d). The inset shows an enlarged m / z range of 362–366 obtained on a MALDI-TOF/TOF.

    Journal: ACS Chemical Neuroscience

    Article Title: Visualizing Neurotransmitters and Metabolites in the Central Nervous System by High Resolution and High Accuracy Mass Spectrometric Imaging

    doi: 10.1021/cn400065k

    Figure Lengend Snippet: Comparison between HRMS- and MRMS-produced spectra acquired from serial rat CNS sections. (a) HRMS-generated spectrum corresponding to the region of interest (ROI) localized in the cortex region of a rat brain section as highlighted in (b). The inset shows an enlarged m / z range of 362–366 obtained on a MALDI LTQ Orbitrap XL. (c) MRMS-generated mass spectrum corresponding to an identical ROI from the cortex region of a serial rat brain section as shown in (d). The inset shows an enlarged m / z range of 362–366 obtained on a MALDI-TOF/TOF.

    Article Snippet: The regional MS spectra and MS images obtained on the MALDI LTQ Orbitrap XL were processed using ImageQuest (Thermo Scientific).

    Techniques: Produced, Generated