magnetic oligo dt beads  (Thermo Fisher)


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    Structured Review

    Thermo Fisher magnetic oligo dt beads
    Magnetic Oligo Dt Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnetic oligo dt beads/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    magnetic oligo dt beads - by Bioz Stars, 2020-05
    93/100 stars

    Related Products / Commonly Used Together

    poly a mrna
    dnase i
    library construction
    total rna

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    Isolation:

    Article Title: Enriched networks ‘nucleoside/nucleotide and ribonucleoside/ribonucleotide metabolic processes’ and ‘response to stimulus’ potentially conferred to drought adaptation of the epiphytic orchid Dendrobium wangliangii
    Article Snippet: .. Briefly, total RNA was treated with DNase I prior to library construction, and poly (A) mRNA was isolated using magnetic oligo (dT) beads (Invitrogen). .. The mRNA was first fragmented by treatment with divalent cations and heat and then transcribed into first-strand cDNA using reverse transcriptase and random hexamer primers, followed by second strand cDNA synthesis using DNA polymerase I and RNase H. The double-stranded cDNA was further end repaired, and indexed adapters were ligated.

    Article Title: Molecular cloning of skin peptide precursor-encoding cDNAs from tibial gland secretion of the Giant Monkey Frog, Phyllomedusa bicolor (Hylidae, Anura).
    Article Snippet: .. The skins of phyllomedusine frogs have long been considered as being tremendously rich sources of bioactive peptides. .. The skins of phyllomedusine frogs have long been considered as being tremendously rich sources of bioactive peptides.

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    Thermo Fisher sera mag oligo dt magnetic beads
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Sera Mag Oligo Dt Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sera mag oligo dt magnetic beads/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    sera mag oligo dt magnetic beads - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher oligo
    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and <t>mRNA</t> is isolated from this using <t>oligo</t> dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.
    Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2039 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo/product/Thermo Fisher
    Average 99 stars, based on 2039 article reviews
    Price from $9.99 to $1999.99
    oligo - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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    Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and mRNA is isolated from this using oligo dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.

    Journal: Frontiers in Plant Science

    Article Title: A High-Throughput Method for Illumina RNA-Seq Library Preparation

    doi: 10.3389/fpls.2012.00202

    Figure Lengend Snippet: Outline of the high-throughput RNA-seq (HTR) library preparation . In short, frozen tissue samples are ground in the lysis buffer and mRNA is isolated from this using oligo dT beads (1). The mRNA is used to make first and second strands of cDNA (2) and this double stranded cDNA molecules are subsequently enzymatically fragmented (3). The ends of these molecules are repaired and an A nucleotide is added (4) to facilitate TA ligation of the barcoded adapters (5). The ligated samples are then enriched by amplification using adapter specific primers (6) and purified for sequencing.

    Article Snippet: For HTR library preparations, mRNA isolation with both Dynabeads (Invitrogen) and Sera-Mag oligo dT magnetic beads (Thermo Scientific, Cat. # 3815-2103-010150) were performed based on the Dynabeads mRNA direct kit (Invitrogen) protocol with minor adjustments (see Methods in Supplementary Material).

    Techniques: High Throughput Screening Assay, RNA Sequencing Assay, Lysis, Isolation, Ligation, Amplification, Purification, Sequencing