magnetic his tag dynabeads  (Thermo Fisher)


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    Structured Review

    Thermo Fisher magnetic his tag dynabeads
    Magnetic His Tag Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnetic his tag dynabeads/product/Thermo Fisher
    Average 82 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    magnetic his tag dynabeads - by Bioz Stars, 2019-12
    82/100 stars

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    Related Articles

    Purification:

    Article Title: Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis
    Article Snippet: Amplified cDNA from Round B was purified using AMPure XP beads (Beckman Coulter, Brea, CA), and 1 μg DNA was used as input into Oxford Nanopore Genomic DNA MAP-003 Kits (Chik1, Ebola1) or MAP-004 Kits (HepC1, Ebola2) for generation of MinION Oxford Nanopore-compatible libraries [ , ]. .. Briefly, the steps include: (1) addition of control lambda phage DNA, (2) end-repair with the NEBNext End Repair Module, (3) 1× AMPure purification, (4) dA-tailing with the NEBNext dA-tailing Module, (5) ligation to protein-linked adapters HP/AMP (Oxford Nanopore Technologies, Oxford, UK) using the NEBNext QuickLigation Module for 10 min at room temperature, (6) purification of ligated libraries using magnetic His-Tag Dynabeads (Life Technologies), and (7) elution in 25 μL buffer (Oxford Nanopore Technologies). .. Lambda phage DNA was not added during preparation of the Ebola2 sample library.

    Amplification:

    Article Title: Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis
    Article Snippet: Amplified cDNA from Round B was purified using AMPure XP beads (Beckman Coulter, Brea, CA), and 1 μg DNA was used as input into Oxford Nanopore Genomic DNA MAP-003 Kits (Chik1, Ebola1) or MAP-004 Kits (HepC1, Ebola2) for generation of MinION Oxford Nanopore-compatible libraries [ , ]. .. Briefly, the steps include: (1) addition of control lambda phage DNA, (2) end-repair with the NEBNext End Repair Module, (3) 1× AMPure purification, (4) dA-tailing with the NEBNext dA-tailing Module, (5) ligation to protein-linked adapters HP/AMP (Oxford Nanopore Technologies, Oxford, UK) using the NEBNext QuickLigation Module for 10 min at room temperature, (6) purification of ligated libraries using magnetic His-Tag Dynabeads (Life Technologies), and (7) elution in 25 μL buffer (Oxford Nanopore Technologies).

    Ligation:

    Article Title: Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis
    Article Snippet: Amplified cDNA from Round B was purified using AMPure XP beads (Beckman Coulter, Brea, CA), and 1 μg DNA was used as input into Oxford Nanopore Genomic DNA MAP-003 Kits (Chik1, Ebola1) or MAP-004 Kits (HepC1, Ebola2) for generation of MinION Oxford Nanopore-compatible libraries [ , ]. .. Briefly, the steps include: (1) addition of control lambda phage DNA, (2) end-repair with the NEBNext End Repair Module, (3) 1× AMPure purification, (4) dA-tailing with the NEBNext dA-tailing Module, (5) ligation to protein-linked adapters HP/AMP (Oxford Nanopore Technologies, Oxford, UK) using the NEBNext QuickLigation Module for 10 min at room temperature, (6) purification of ligated libraries using magnetic His-Tag Dynabeads (Life Technologies), and (7) elution in 25 μL buffer (Oxford Nanopore Technologies). .. Lambda phage DNA was not added during preparation of the Ebola2 sample library.

    Nanopore Sequencing:

    Article Title: Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis
    Article Snippet: Paragraph title: Preparation of nanopore sequencing libraries ... Briefly, the steps include: (1) addition of control lambda phage DNA, (2) end-repair with the NEBNext End Repair Module, (3) 1× AMPure purification, (4) dA-tailing with the NEBNext dA-tailing Module, (5) ligation to protein-linked adapters HP/AMP (Oxford Nanopore Technologies, Oxford, UK) using the NEBNext QuickLigation Module for 10 min at room temperature, (6) purification of ligated libraries using magnetic His-Tag Dynabeads (Life Technologies), and (7) elution in 25 μL buffer (Oxford Nanopore Technologies).

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    Thermo Fisher coated magnetic dynabeads
    Nanobody-coated beads agglutinate C. jejuni KC40 cells. The His-tagged nanobodies were coupled to magnetic <t>dynabeads,</t> leading to multimerization. A Nb84 coupled to dynabeads causes agglutination of KC40 cells. B As a negative control, dynabeads coated with anti- E. coli nanobodies were mixed with KC40 cells. No agglutination was observed in this case. C The C. jejuni KC40 bacteria and D the beads coated with Nb84. The results were observed by phase contrast microscopy, using a ×100 oil immersion objective.
    Coated Magnetic Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coated magnetic dynabeads/product/Thermo Fisher
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    coated magnetic dynabeads - by Bioz Stars, 2019-12
    77/100 stars
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    81
    Thermo Fisher his tag dynabeadstm magnetic beads
    Nanobody-coated beads agglutinate C. jejuni KC40 cells. The His-tagged nanobodies were coupled to magnetic <t>dynabeads,</t> leading to multimerization. A Nb84 coupled to dynabeads causes agglutination of KC40 cells. B As a negative control, dynabeads coated with anti- E. coli nanobodies were mixed with KC40 cells. No agglutination was observed in this case. C The C. jejuni KC40 bacteria and D the beads coated with Nb84. The results were observed by phase contrast microscopy, using a ×100 oil immersion objective.
    His Tag Dynabeadstm Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/his tag dynabeadstm magnetic beads/product/Thermo Fisher
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    his tag dynabeadstm magnetic beads - by Bioz Stars, 2019-12
    81/100 stars
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    Nanobody-coated beads agglutinate C. jejuni KC40 cells. The His-tagged nanobodies were coupled to magnetic dynabeads, leading to multimerization. A Nb84 coupled to dynabeads causes agglutination of KC40 cells. B As a negative control, dynabeads coated with anti- E. coli nanobodies were mixed with KC40 cells. No agglutination was observed in this case. C The C. jejuni KC40 bacteria and D the beads coated with Nb84. The results were observed by phase contrast microscopy, using a ×100 oil immersion objective.

    Journal: Veterinary Research

    Article Title: Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization

    doi: 10.1186/s13567-017-0491-9

    Figure Lengend Snippet: Nanobody-coated beads agglutinate C. jejuni KC40 cells. The His-tagged nanobodies were coupled to magnetic dynabeads, leading to multimerization. A Nb84 coupled to dynabeads causes agglutination of KC40 cells. B As a negative control, dynabeads coated with anti- E. coli nanobodies were mixed with KC40 cells. No agglutination was observed in this case. C The C. jejuni KC40 bacteria and D the beads coated with Nb84. The results were observed by phase contrast microscopy, using a ×100 oil immersion objective.

    Article Snippet: Selected nanobodies were coupled to Co2+ -coated magnetic Dynabeads (Dynabeads® His-Tag Isolation and Pulldown, Thermo Fisher Scientific) to make them multivalent, using the buffers described in the manual.

    Techniques: Agglutination, Negative Control, Microscopy