magnesium sulfate  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Millipore magnesium sulfate
    Magnesium Sulfate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnesium sulfate/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    magnesium sulfate - by Bioz Stars, 2020-02
    99/100 stars

    Images

    Related Articles

    Mass Measurement:

    Article Title: A method for measuring sulfide toxicity in the nematode Caenorhabditis elegans
    Article Snippet: Analytical balance (Mettler-Toledo) Bacto agar (BD-Difco, Cat. No. 214010) Bacto peptone (BD-Difco, Cat. No. 211677) Bacto tryptone (BD-Difco, Cat. No. 211705) Bacto yeast extract (BD-Difco, Cat. No. 212750) Calcium chloride (Sigma, Cat. No. C1016) Cholesterol (Sigma, Cat. No. C8667) Corning Costar 96 well cell culture plate Corning® 500 ml vacuum filter/storage bottle system, 0.22 μm Costar® 5 ml serological pipettes, (Corning Inc, Product #4487) Dipotassium hydrogen phosphate trihydrate (Sigma, Cat. No. P3786) Double distilled water (DDW) ELMI Intelli RM-2L mixer Heating bath (Lab Companion BW-10H) Heraeus™ Megafuge™ 16 centrifuge (ThermoFisher Scientific) Hydrochloric acid (Sigma, Cat. No. 30721) Incubating shaker (Lab Companion SIF-5000R, Jeio Tech, Korea) Iron chloride (Sigma, Cat. No. 157740) L-Cysteine (Sigma-Aldrich Cat. No. W326305). .. Magnesium sulfate (Sigma, Cat. No. M2670) Microlance hypodermic needle 21G x 1.5” (BD 304432) Minisart 0.2 μm syringe filter (Sartorius Stedim Biotech, Cat. No. 16534) N ,N -Dimethyl-p -phenylenediamine (Sigma, Cat. No. 186384) Nunc 200 ml centrifuge tube (Thermo Fisher Scientific, Cat. No. 376813) Olympus SZ61 stereo microscope (Olympus) Parafilm roll (Bemis, USA, Cat. No. PM-996) Petri dish, 35 × 10 mm (Runlab Labware Manufacturing Co., Taizhou, China, Cat. No. 55801). pH meter (Mettler-Toledo) Platinum/iridium 0.25 mm wire (World Precision Instruments, Inc., Cat. No. PT1002) Potassium dihydrogen phosphate (Merck, Cat. No. 1.04873.1000) Sodium chloride (DAEJUNG Cat. No. 7548-4400) Sodium hydroxide (Gadot, Cat. No. 830224310) Sodium hypochlorite solution (Sigma, Cat. No. 239305) Sodium sulfide (Sigma, Cat. No. 208043) Tecan® Infinite® 200 PRO multimode microplate reader (Tecan Trading AG, Switzerland) Tuttnauer cGMP pharmaceutical autoclave- stream sterilizer, model 5596 Compact Vortex (Lab Companion AAH360115U) Zinc acetate (Sigma, Cat. No. 383317)

    Microscopy:

    Article Title: A method for measuring sulfide toxicity in the nematode Caenorhabditis elegans
    Article Snippet: .. Magnesium sulfate (Sigma, Cat. No. M2670) Microlance hypodermic needle 21G x 1.5” (BD 304432) Minisart 0.2 μm syringe filter (Sartorius Stedim Biotech, Cat. No. 16534) N ,N -Dimethyl-p -phenylenediamine (Sigma, Cat. No. 186384) Nunc 200 ml centrifuge tube (Thermo Fisher Scientific, Cat. No. 376813) Olympus SZ61 stereo microscope (Olympus) Parafilm roll (Bemis, USA, Cat. No. PM-996) Petri dish, 35 × 10 mm (Runlab Labware Manufacturing Co., Taizhou, China, Cat. No. 55801). pH meter (Mettler-Toledo) Platinum/iridium 0.25 mm wire (World Precision Instruments, Inc., Cat. No. PT1002) Potassium dihydrogen phosphate (Merck, Cat. No. 1.04873.1000) Sodium chloride (DAEJUNG Cat. No. 7548-4400) Sodium hydroxide (Gadot, Cat. No. 830224310) Sodium hypochlorite solution (Sigma, Cat. No. 239305) Sodium sulfide (Sigma, Cat. No. 208043) Tecan® Infinite® 200 PRO multimode microplate reader (Tecan Trading AG, Switzerland) Tuttnauer cGMP pharmaceutical autoclave- stream sterilizer, model 5596 Compact Vortex (Lab Companion AAH360115U) Zinc acetate (Sigma, Cat. No. 383317) ..

    Cell Culture:

    Article Title: A method for measuring sulfide toxicity in the nematode Caenorhabditis elegans
    Article Snippet: Analytical balance (Mettler-Toledo) Bacto agar (BD-Difco, Cat. No. 214010) Bacto peptone (BD-Difco, Cat. No. 211677) Bacto tryptone (BD-Difco, Cat. No. 211705) Bacto yeast extract (BD-Difco, Cat. No. 212750) Calcium chloride (Sigma, Cat. No. C1016) Cholesterol (Sigma, Cat. No. C8667) Corning Costar 96 well cell culture plate Corning® 500 ml vacuum filter/storage bottle system, 0.22 μm Costar® 5 ml serological pipettes, (Corning Inc, Product #4487) Dipotassium hydrogen phosphate trihydrate (Sigma, Cat. No. P3786) Double distilled water (DDW) ELMI Intelli RM-2L mixer Heating bath (Lab Companion BW-10H) Heraeus™ Megafuge™ 16 centrifuge (ThermoFisher Scientific) Hydrochloric acid (Sigma, Cat. No. 30721) Incubating shaker (Lab Companion SIF-5000R, Jeio Tech, Korea) Iron chloride (Sigma, Cat. No. 157740) L-Cysteine (Sigma-Aldrich Cat. No. W326305). .. Magnesium sulfate (Sigma, Cat. No. M2670) Microlance hypodermic needle 21G x 1.5” (BD 304432) Minisart 0.2 μm syringe filter (Sartorius Stedim Biotech, Cat. No. 16534) N ,N -Dimethyl-p -phenylenediamine (Sigma, Cat. No. 186384) Nunc 200 ml centrifuge tube (Thermo Fisher Scientific, Cat. No. 376813) Olympus SZ61 stereo microscope (Olympus) Parafilm roll (Bemis, USA, Cat. No. PM-996) Petri dish, 35 × 10 mm (Runlab Labware Manufacturing Co., Taizhou, China, Cat. No. 55801). pH meter (Mettler-Toledo) Platinum/iridium 0.25 mm wire (World Precision Instruments, Inc., Cat. No. PT1002) Potassium dihydrogen phosphate (Merck, Cat. No. 1.04873.1000) Sodium chloride (DAEJUNG Cat. No. 7548-4400) Sodium hydroxide (Gadot, Cat. No. 830224310) Sodium hypochlorite solution (Sigma, Cat. No. 239305) Sodium sulfide (Sigma, Cat. No. 208043) Tecan® Infinite® 200 PRO multimode microplate reader (Tecan Trading AG, Switzerland) Tuttnauer cGMP pharmaceutical autoclave- stream sterilizer, model 5596 Compact Vortex (Lab Companion AAH360115U) Zinc acetate (Sigma, Cat. No. 383317)

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Millipore alcian blue staining
    Meclozine promotes chondrocyte proliferation and ameliorates loss of extracellular matrix in FGF2-treated <t>RCS</t> cells. (A, B) RCS cells were treated with 5 ng/ml FGF2 and the indicated concentrations of meclozine for 48 hours. Cell growth was quantified using the MTS assay (A) or by counting cells (B). Data are normalized to that without meclozine and indicated by the mean and SD ( n = 8 for A and 6 for B). Meclozine rescued the FGF2-mediated growth arrest of RCS cells. (C) Meclozine (10 µM) ameliorated FGF2-mediated alteration of cellular shape and loss of extracellular matrix. RCS cells were treated with 5 ng/ml FGF2 with and without 0.2 µM CNP or 20 µM meclozine for 72 hours, and cartilage-like sulfated proteoglycan matrix was stained by <t>Alcian</t> blue. Growing RCS cells were round-shaped and produced abundant cartilage-like sulfated proteoglycan matrix in the absence of FGF2. FGF2 treatment transformed some cells to fibroblast-like shapes and prominently suppressed expression of sulfated proteoglycan matrix. In the RCS cells treated with CNP or meclozine, the cellular shape remained round and the intensity of Alcian blue staining approximated that of FGF2-negative cells. Representative images of triplicated experiments are shown. Magnified images of the middle panels are shown in the rightmost column. Bars in the left, middle, and right panels are 750, 150, and 30 µm, respectively. (D) Meclozine (20 µM) inhibited mRNA expression of matrix metalloproteinases in FGF2-treated RCS cells. Cells were treated with FGF2 and either CNP or meclozine for four hours and mRNAs were quantified by real-time RT-PCR. Expression levels of Mmp10 , Mmp13 , and Adamts1 are presented as the mean and SD normalized to that of FGF2-negative cells ( n = 3). FGF2-mediated increases of Mmp10 , Mmp13 , and Adamts1 mRNA were antagonized by CNP and meclozine. Statistical significance is estimated by Student's t-test.
    Alcian Blue Staining, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alcian blue staining/product/Millipore
    Average 95 stars, based on 122 article reviews
    Price from $9.99 to $1999.99
    alcian blue staining - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    Millipore m magnesium sulfate
    Meclozine promotes chondrocyte proliferation and ameliorates loss of extracellular matrix in FGF2-treated <t>RCS</t> cells. (A, B) RCS cells were treated with 5 ng/ml FGF2 and the indicated concentrations of meclozine for 48 hours. Cell growth was quantified using the MTS assay (A) or by counting cells (B). Data are normalized to that without meclozine and indicated by the mean and SD ( n = 8 for A and 6 for B). Meclozine rescued the FGF2-mediated growth arrest of RCS cells. (C) Meclozine (10 µM) ameliorated FGF2-mediated alteration of cellular shape and loss of extracellular matrix. RCS cells were treated with 5 ng/ml FGF2 with and without 0.2 µM CNP or 20 µM meclozine for 72 hours, and cartilage-like sulfated proteoglycan matrix was stained by <t>Alcian</t> blue. Growing RCS cells were round-shaped and produced abundant cartilage-like sulfated proteoglycan matrix in the absence of FGF2. FGF2 treatment transformed some cells to fibroblast-like shapes and prominently suppressed expression of sulfated proteoglycan matrix. In the RCS cells treated with CNP or meclozine, the cellular shape remained round and the intensity of Alcian blue staining approximated that of FGF2-negative cells. Representative images of triplicated experiments are shown. Magnified images of the middle panels are shown in the rightmost column. Bars in the left, middle, and right panels are 750, 150, and 30 µm, respectively. (D) Meclozine (20 µM) inhibited mRNA expression of matrix metalloproteinases in FGF2-treated RCS cells. Cells were treated with FGF2 and either CNP or meclozine for four hours and mRNAs were quantified by real-time RT-PCR. Expression levels of Mmp10 , Mmp13 , and Adamts1 are presented as the mean and SD normalized to that of FGF2-negative cells ( n = 3). FGF2-mediated increases of Mmp10 , Mmp13 , and Adamts1 mRNA were antagonized by CNP and meclozine. Statistical significance is estimated by Student's t-test.
    M Magnesium Sulfate, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m magnesium sulfate/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m magnesium sulfate - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier


    Image Search Results


    Meclozine promotes chondrocyte proliferation and ameliorates loss of extracellular matrix in FGF2-treated RCS cells. (A, B) RCS cells were treated with 5 ng/ml FGF2 and the indicated concentrations of meclozine for 48 hours. Cell growth was quantified using the MTS assay (A) or by counting cells (B). Data are normalized to that without meclozine and indicated by the mean and SD ( n = 8 for A and 6 for B). Meclozine rescued the FGF2-mediated growth arrest of RCS cells. (C) Meclozine (10 µM) ameliorated FGF2-mediated alteration of cellular shape and loss of extracellular matrix. RCS cells were treated with 5 ng/ml FGF2 with and without 0.2 µM CNP or 20 µM meclozine for 72 hours, and cartilage-like sulfated proteoglycan matrix was stained by Alcian blue. Growing RCS cells were round-shaped and produced abundant cartilage-like sulfated proteoglycan matrix in the absence of FGF2. FGF2 treatment transformed some cells to fibroblast-like shapes and prominently suppressed expression of sulfated proteoglycan matrix. In the RCS cells treated with CNP or meclozine, the cellular shape remained round and the intensity of Alcian blue staining approximated that of FGF2-negative cells. Representative images of triplicated experiments are shown. Magnified images of the middle panels are shown in the rightmost column. Bars in the left, middle, and right panels are 750, 150, and 30 µm, respectively. (D) Meclozine (20 µM) inhibited mRNA expression of matrix metalloproteinases in FGF2-treated RCS cells. Cells were treated with FGF2 and either CNP or meclozine for four hours and mRNAs were quantified by real-time RT-PCR. Expression levels of Mmp10 , Mmp13 , and Adamts1 are presented as the mean and SD normalized to that of FGF2-negative cells ( n = 3). FGF2-mediated increases of Mmp10 , Mmp13 , and Adamts1 mRNA were antagonized by CNP and meclozine. Statistical significance is estimated by Student's t-test.

    Journal: PLoS ONE

    Article Title: Meclozine Facilitates Proliferation and Differentiation of Chondrocytes by Attenuating Abnormally Activated FGFR3 Signaling in Achondroplasia

    doi: 10.1371/journal.pone.0081569

    Figure Lengend Snippet: Meclozine promotes chondrocyte proliferation and ameliorates loss of extracellular matrix in FGF2-treated RCS cells. (A, B) RCS cells were treated with 5 ng/ml FGF2 and the indicated concentrations of meclozine for 48 hours. Cell growth was quantified using the MTS assay (A) or by counting cells (B). Data are normalized to that without meclozine and indicated by the mean and SD ( n = 8 for A and 6 for B). Meclozine rescued the FGF2-mediated growth arrest of RCS cells. (C) Meclozine (10 µM) ameliorated FGF2-mediated alteration of cellular shape and loss of extracellular matrix. RCS cells were treated with 5 ng/ml FGF2 with and without 0.2 µM CNP or 20 µM meclozine for 72 hours, and cartilage-like sulfated proteoglycan matrix was stained by Alcian blue. Growing RCS cells were round-shaped and produced abundant cartilage-like sulfated proteoglycan matrix in the absence of FGF2. FGF2 treatment transformed some cells to fibroblast-like shapes and prominently suppressed expression of sulfated proteoglycan matrix. In the RCS cells treated with CNP or meclozine, the cellular shape remained round and the intensity of Alcian blue staining approximated that of FGF2-negative cells. Representative images of triplicated experiments are shown. Magnified images of the middle panels are shown in the rightmost column. Bars in the left, middle, and right panels are 750, 150, and 30 µm, respectively. (D) Meclozine (20 µM) inhibited mRNA expression of matrix metalloproteinases in FGF2-treated RCS cells. Cells were treated with FGF2 and either CNP or meclozine for four hours and mRNAs were quantified by real-time RT-PCR. Expression levels of Mmp10 , Mmp13 , and Adamts1 are presented as the mean and SD normalized to that of FGF2-negative cells ( n = 3). FGF2-mediated increases of Mmp10 , Mmp13 , and Adamts1 mRNA were antagonized by CNP and meclozine. Statistical significance is estimated by Student's t-test.

    Article Snippet: Alcian blue staining For Alcian blue staining, ∼1×105 RCS cells in a 12-well plate were added with 5 ng/ml FGF2 and also with either 10 µM meclozine or 0.2 µM CNP (Calbiochem).

    Techniques: MTS Assay, Staining, Produced, Transformation Assay, Expressing, Quantitative RT-PCR

    In vitro differentiation potential of NCSCs. (A–B) Immunostaining for Schwann cell markers S100β (A) and GFAP (B). (C–D) Immunostaining for peripheral neuron markers peripherin (C) and Tuj1 (D). (E–F) Immunostaining for SMC lineage markers CNN1 (E) and SMA (F). (G–H) Adipogenic differentiation shown by phase contrast imaging (G) and Oil red staining (H). (I–J) Osteogenic differentiation shown by Alizarin red staining for calcified matrix (G) and immunostaining of alkaline phosphatase (ALP) (H). (K–L) Chondrogenic differentiation shown by Alcian blue staining for glycosaminoglycans (E) and immunofluorescent staining of Col-II (F). In all immunofluorescence images, nuclei were stained by DAPI in blue. Scale bar = 100 µm.

    Journal: PLoS ONE

    Article Title: Uniaxial Mechanical Strain Modulates the Differentiation of Neural Crest Stem Cells into Smooth Muscle Lineage on Micropatterned Surfaces

    doi: 10.1371/journal.pone.0026029

    Figure Lengend Snippet: In vitro differentiation potential of NCSCs. (A–B) Immunostaining for Schwann cell markers S100β (A) and GFAP (B). (C–D) Immunostaining for peripheral neuron markers peripherin (C) and Tuj1 (D). (E–F) Immunostaining for SMC lineage markers CNN1 (E) and SMA (F). (G–H) Adipogenic differentiation shown by phase contrast imaging (G) and Oil red staining (H). (I–J) Osteogenic differentiation shown by Alizarin red staining for calcified matrix (G) and immunostaining of alkaline phosphatase (ALP) (H). (K–L) Chondrogenic differentiation shown by Alcian blue staining for glycosaminoglycans (E) and immunofluorescent staining of Col-II (F). In all immunofluorescence images, nuclei were stained by DAPI in blue. Scale bar = 100 µm.

    Article Snippet: The cell pellets were cryosectioned, and stained for glycosaminoglycans (GAGs) by using Alcian blue staining or immunostained for Collagen type II (Col-II; MAB8887, Millipore Corp.).

    Techniques: In Vitro, Immunostaining, Imaging, Staining, ALP Assay, Immunofluorescence