machr antibodies  (Alomone Labs)


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    Structured Review

    Alomone Labs machr antibodies
    Paralysis initiates normal sprouting in <t>mAChR</t> KO mice. LAL muscles of wildtype (+/+), <t>M1/3-/-,</t> M2/4-/-, and M5-/- mice were paralyzed for 7 days and labeled as described in . ( A ), representative junctions of +/+, M2/4 -/- and M5-/- NMJs exhibiting
    Machr Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/machr antibodies/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    machr antibodies - by Bioz Stars, 2022-10
    85/100 stars

    Images

    1) Product Images from "Distinct muscarinic acetylcholine receptor subtypes contribute to stability and growth, but not compensatory plasticity, of neuromuscular synapses"

    Article Title: Distinct muscarinic acetylcholine receptor subtypes contribute to stability and growth, but not compensatory plasticity, of neuromuscular synapses

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    doi: 10.1523/JNEUROSCI.2276-09.2009

    Paralysis initiates normal sprouting in mAChR KO mice. LAL muscles of wildtype (+/+), M1/3-/-, M2/4-/-, and M5-/- mice were paralyzed for 7 days and labeled as described in . ( A ), representative junctions of +/+, M2/4 -/- and M5-/- NMJs exhibiting
    Figure Legend Snippet: Paralysis initiates normal sprouting in mAChR KO mice. LAL muscles of wildtype (+/+), M1/3-/-, M2/4-/-, and M5-/- mice were paralyzed for 7 days and labeled as described in . ( A ), representative junctions of +/+, M2/4 -/- and M5-/- NMJs exhibiting

    Techniques Used: Mouse Assay, Labeling

    Localization of mAChR subtypes at NMJs. (A) , Although an anti M2 antibody labels axon terminals, tSCs, and postsynaptic gutters in wildtype NMJs, nerve terminal-associated labeling (arrow, inset) disappears in M2-/- NMJs, whereas labeling associated with
    Figure Legend Snippet: Localization of mAChR subtypes at NMJs. (A) , Although an anti M2 antibody labels axon terminals, tSCs, and postsynaptic gutters in wildtype NMJs, nerve terminal-associated labeling (arrow, inset) disappears in M2-/- NMJs, whereas labeling associated with

    Techniques Used: Labeling

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    Alomone Labs chrm3
    Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( <t>Chrm3</t> ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p
    Chrm3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chrm3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chrm3 - by Bioz Stars, 2022-10
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    93
    Alomone Labs anti machr m2 antibody
    Expression of mAChRs transcript in the transverse colon. The levels of <t>mAChR</t> M2 and M3 transcripts in the total mRNA of transverse colons were measured by RT-PCR using specific primers. After the intensity of each band was determined using an imaging densitometer, the relative levels of mAChR M2 and M3 transcripts were calculated based on the intensity of actin transcripts. Five to six rats per group were assayed in triplicate by RT-PCR assays. Data represent the means±SD of three replicates. a, p
    Anti Machr M2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti machr m2 antibody/product/Alomone Labs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti machr m2 antibody - by Bioz Stars, 2022-10
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    85
    Alomone Labs machr antibodies
    Paralysis initiates normal sprouting in <t>mAChR</t> KO mice. LAL muscles of wildtype (+/+), <t>M1/3-/-,</t> M2/4-/-, and M5-/- mice were paralyzed for 7 days and labeled as described in . ( A ), representative junctions of +/+, M2/4 -/- and M5-/- NMJs exhibiting
    Machr Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/machr antibodies/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    machr antibodies - by Bioz Stars, 2022-10
    85/100 stars
      Buy from Supplier

    Image Search Results


    Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p

    Journal: Scientific Reports

    Article Title: Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels

    doi: 10.1038/s41598-019-52811-4

    Figure Lengend Snippet: Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p

    Article Snippet: Protein levels were assessed from 50 μg of extracts separated using 10% SDS-PAGE gels by probing with antibodies specific to P2rx1 (APR-001, Alomone Labs), Chrm2 (AMR-002, Alomone Labs,), Chrm3 (AMR-006, Alomone Labs, Israel), α-SMA (ab5694, Rabbit, Abcam), vimentin (sc-6260, Santa Cruz Biotechnology Inc., CA), and β-actin (Sigma-Aldrich).

    Techniques: Polymerase Chain Reaction, Western Blot, Expressing, Immunohistochemistry, Staining

    Expression of mAChRs transcript in the transverse colon. The levels of mAChR M2 and M3 transcripts in the total mRNA of transverse colons were measured by RT-PCR using specific primers. After the intensity of each band was determined using an imaging densitometer, the relative levels of mAChR M2 and M3 transcripts were calculated based on the intensity of actin transcripts. Five to six rats per group were assayed in triplicate by RT-PCR assays. Data represent the means±SD of three replicates. a, p

    Journal: PLoS ONE

    Article Title: Gallotannin-Enriched Extract Isolated from Galla Rhois May Be a Functional Candidate with Laxative Effects for Treatment of Loperamide-Induced Constipation of SD Rats

    doi: 10.1371/journal.pone.0161144

    Figure Lengend Snippet: Expression of mAChRs transcript in the transverse colon. The levels of mAChR M2 and M3 transcripts in the total mRNA of transverse colons were measured by RT-PCR using specific primers. After the intensity of each band was determined using an imaging densitometer, the relative levels of mAChR M2 and M3 transcripts were calculated based on the intensity of actin transcripts. Five to six rats per group were assayed in triplicate by RT-PCR assays. Data represent the means±SD of three replicates. a, p

    Article Snippet: Western blotting Total proteins collected from the transverse colons of subset groups (No, GEGR, Lop+vehicle, Lop+LoGEGR, Lop+MeGEGR, Lop+HiGEGR and Lop+BS treated SD rats) were separated by 4%–20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for 3 h, after which the resolved proteins were transferred to nitrocellulose membranes for 2 h at 40 V. Each membrane was then incubated separately with primary antibody, anti-mAChR M2 antibody (Alomone Labs, Jerusalem, Israel), anti-PI-3K (Cell Signaling Technology Inc., Cambridge, MA, USA), anti-p-PI3K (Cell Signaling Technology Inc., Cambridge, MA, USA), anti-mAChR M3 antibody (Alomone Labs, Jerusalem, Israel), anti-PKC (Cell Signaling Technology Inc.), anti-p-PKC (Cell Signaling Technology Inc.), anti-Gα (Abcame, Cambridge, UK) or anti-actin (Sigma-Aldrich Co.) overnight at 4°C.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Imaging

    Expression of key proteins in the mAChR M2 and M3 signaling pathway. The expression of several related proteins in the mAChR M2 and M3 signaling pathway was measured by Western blot analysis using HRP-labeled anti-rabbit IgG antibody. After the intensity of each band was determined using an imaging densitometer, the relative levels of six proteins were calculated based on the intensity of actin protein. Five to six rats per group were assayed in triplicate by Western blotting. Data represent the means ± SD of three replicates. a, p

    Journal: PLoS ONE

    Article Title: Gallotannin-Enriched Extract Isolated from Galla Rhois May Be a Functional Candidate with Laxative Effects for Treatment of Loperamide-Induced Constipation of SD Rats

    doi: 10.1371/journal.pone.0161144

    Figure Lengend Snippet: Expression of key proteins in the mAChR M2 and M3 signaling pathway. The expression of several related proteins in the mAChR M2 and M3 signaling pathway was measured by Western blot analysis using HRP-labeled anti-rabbit IgG antibody. After the intensity of each band was determined using an imaging densitometer, the relative levels of six proteins were calculated based on the intensity of actin protein. Five to six rats per group were assayed in triplicate by Western blotting. Data represent the means ± SD of three replicates. a, p

    Article Snippet: Western blotting Total proteins collected from the transverse colons of subset groups (No, GEGR, Lop+vehicle, Lop+LoGEGR, Lop+MeGEGR, Lop+HiGEGR and Lop+BS treated SD rats) were separated by 4%–20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for 3 h, after which the resolved proteins were transferred to nitrocellulose membranes for 2 h at 40 V. Each membrane was then incubated separately with primary antibody, anti-mAChR M2 antibody (Alomone Labs, Jerusalem, Israel), anti-PI-3K (Cell Signaling Technology Inc., Cambridge, MA, USA), anti-p-PI3K (Cell Signaling Technology Inc., Cambridge, MA, USA), anti-mAChR M3 antibody (Alomone Labs, Jerusalem, Israel), anti-PKC (Cell Signaling Technology Inc.), anti-p-PKC (Cell Signaling Technology Inc.), anti-Gα (Abcame, Cambridge, UK) or anti-actin (Sigma-Aldrich Co.) overnight at 4°C.

    Techniques: Expressing, Western Blot, Labeling, Imaging

    Immunostaining of HL-1 cells. Immunofluorescent staining of HL-1 cells and the M2 GIRK1/4 cell line stained for GIRK4 and the M2 receptor, respectively.  Arrows  show potential membrane localisation. In the  lower panels  of the figure, two controls are shown. In parental HEK293 cells without GIRK4 expression, the GIRK4 antibody does not stain the cells. Incubation of the M2 GIRK1/4 cell line with only the secondary antibody and not the primary does not lead to staining

    Journal: Pflugers Archiv

    Article Title: HL-1 cells express an inwardly rectifying K+ current activated via muscarinic receptors comparable to that in mouse atrial myocytes

    doi: 10.1007/s00424-010-0799-z

    Figure Lengend Snippet: Immunostaining of HL-1 cells. Immunofluorescent staining of HL-1 cells and the M2 GIRK1/4 cell line stained for GIRK4 and the M2 receptor, respectively. Arrows show potential membrane localisation. In the lower panels of the figure, two controls are shown. In parental HEK293 cells without GIRK4 expression, the GIRK4 antibody does not stain the cells. Incubation of the M2 GIRK1/4 cell line with only the secondary antibody and not the primary does not lead to staining

    Article Snippet: GIRK4 (APC-027, Alomone Laboratories) and M2 receptor (AMR-002, Alomone Laboratories) antibodies were used at a final dilution of 1/500.

    Techniques: Immunostaining, Staining, Expressing, Incubation

    K + current activated by carbachol in HEK293 stable cell line. Carbachol activates an inwardly rectifying K + current in a stable HEK293 cell line expressing the M2 receptor and the GIRK1 and GIRK4 channel subunits. Sample voltage-clamp recordings and current voltage relationships are shown

    Journal: Pflugers Archiv

    Article Title: HL-1 cells express an inwardly rectifying K+ current activated via muscarinic receptors comparable to that in mouse atrial myocytes

    doi: 10.1007/s00424-010-0799-z

    Figure Lengend Snippet: K + current activated by carbachol in HEK293 stable cell line. Carbachol activates an inwardly rectifying K + current in a stable HEK293 cell line expressing the M2 receptor and the GIRK1 and GIRK4 channel subunits. Sample voltage-clamp recordings and current voltage relationships are shown

    Article Snippet: GIRK4 (APC-027, Alomone Laboratories) and M2 receptor (AMR-002, Alomone Laboratories) antibodies were used at a final dilution of 1/500.

    Techniques: Stable Transfection, Expressing

    Paralysis initiates normal sprouting in mAChR KO mice. LAL muscles of wildtype (+/+), M1/3-/-, M2/4-/-, and M5-/- mice were paralyzed for 7 days and labeled as described in . ( A ), representative junctions of +/+, M2/4 -/- and M5-/- NMJs exhibiting

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Distinct muscarinic acetylcholine receptor subtypes contribute to stability and growth, but not compensatory plasticity, of neuromuscular synapses

    doi: 10.1523/JNEUROSCI.2276-09.2009

    Figure Lengend Snippet: Paralysis initiates normal sprouting in mAChR KO mice. LAL muscles of wildtype (+/+), M1/3-/-, M2/4-/-, and M5-/- mice were paralyzed for 7 days and labeled as described in . ( A ), representative junctions of +/+, M2/4 -/- and M5-/- NMJs exhibiting

    Article Snippet: Sources of mAChR antibodies were as follows: anti-M1, -M2, and -M3 rabbit polyclonal antibodies (Alomone Labs, Jerusalem, Israel); anti-M2 monoclonal and anti-M5 rabbit polyclonal antibodies (Abcam Inc. Cambridge, MA); anti-M4 polyclonal antibody (Santa Cruz Biotechnology Inc., Santa Cruz, CA.).

    Techniques: Mouse Assay, Labeling

    Localization of mAChR subtypes at NMJs. (A) , Although an anti M2 antibody labels axon terminals, tSCs, and postsynaptic gutters in wildtype NMJs, nerve terminal-associated labeling (arrow, inset) disappears in M2-/- NMJs, whereas labeling associated with

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Distinct muscarinic acetylcholine receptor subtypes contribute to stability and growth, but not compensatory plasticity, of neuromuscular synapses

    doi: 10.1523/JNEUROSCI.2276-09.2009

    Figure Lengend Snippet: Localization of mAChR subtypes at NMJs. (A) , Although an anti M2 antibody labels axon terminals, tSCs, and postsynaptic gutters in wildtype NMJs, nerve terminal-associated labeling (arrow, inset) disappears in M2-/- NMJs, whereas labeling associated with

    Article Snippet: Sources of mAChR antibodies were as follows: anti-M1, -M2, and -M3 rabbit polyclonal antibodies (Alomone Labs, Jerusalem, Israel); anti-M2 monoclonal and anti-M5 rabbit polyclonal antibodies (Abcam Inc. Cambridge, MA); anti-M4 polyclonal antibody (Santa Cruz Biotechnology Inc., Santa Cruz, CA.).

    Techniques: Labeling