mace kit  (GenXPro)

 
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    GenXPro mace kit
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mace kit/product/GenXPro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mace kit - by Bioz Stars, 2021-04
    86/100 stars

    Images

    1) Product Images from "NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC"

    Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2017.44

    NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Figure Legend Snippet: NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Techniques Used: Next-Generation Sequencing, Quantitative RT-PCR, Sequencing, Expressing

    Related Articles

    other:

    Article Title: A combined transcriptomic approach to identify candidates for an anti-tick vaccine blocking B. afzelii transmission
    Article Snippet: MACE analysis Essentially, MACE analysis was performed as previously described (Nold-Petry et al. Mueller et al.) using the GenXPro MACE kit (GenXPro, Frankfurt am Main, Germany) and according to the manufacturer’s protocol.

    Construct:

    Article Title: Contribution of brain pericytes in blood–brain barrier formation and maintenance: a transcriptomic study of cocultured human endothelial cells derived from hematopoietic stem cells
    Article Snippet: Preparation of a next-generation sequencing library and subsequent RNA sequencing was performed at GenXPro GmbH. .. A number of 27 MACE libraries was constructed using the MACE-Seq kit v2.0 (GenXPro GmbH) according to the supplier’s protocol. ..

    Article Title: In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
    Article Snippet: Preparation and sequencing of massive analysis of cDNA ends (MACE) libraries MACE libraries were produced for each replicate and treatment separately. .. The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual. ..

    Article Title: Polar Infection of Echovirus-30 Causes Differential Barrier Affection and Gene Regulation at the Blood–Cerebrospinal Fluid Barrier
    Article Snippet: RNA sequencing libraries were analyzed using NGS. .. Fifteen MACE libraries were constructed using the MACE-Seq kit v. 2.0 (GenXPro GmbH, Frankfurt, Germany), according to the manufacturer’s protocol. ..

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    • mace kit  (GenXPro)
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    GenXPro mace kit
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mace kit/product/GenXPro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mace kit - by Bioz Stars, 2021-04
    86/100 stars
    		  Buy from Supplier
    genxpro s mace kit  (GenXPro)
    86
    GenXPro genxpro s mace kit
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Genxpro S Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genxpro s mace kit/product/GenXPro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genxpro s mace kit - by Bioz Stars, 2021-04
    86/100 stars
    		  Buy from Supplier
    mace seq kit v2 0  (GenXPro)
    86
    GenXPro mace seq kit v2 0
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Mace Seq Kit V2 0, supplied by GenXPro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mace seq kit v2 0/product/GenXPro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mace seq kit v2 0 - by Bioz Stars, 2021-04
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Journal: Cell Death & Disease

    Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC

    doi: 10.1038/cddis.2017.44

    Figure Lengend Snippet: NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described.

    Techniques: Next-Generation Sequencing, Quantitative RT-PCR, Sequencing, Expressing