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GenXPro mace kit
<t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace kit/product/GenXPro
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mace kit - by Bioz Stars, 2020-02
93/100 stars

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1) Product Images from "NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC"

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC

Journal: Cell Death & Disease

doi: 10.1038/cddis.2017.44

NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
Figure Legend Snippet: NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

Techniques Used: Next-Generation Sequencing, Quantitative RT-PCR, Sequencing, Expressing

Related Articles

Amplification:

Article Title: In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
Article Snippet: The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual. .. In order to avoid bias during the PCR amplification steps included in the library preparation, GenXPro's PCR-bias-proof technology “TrueQuant,” implemented in the MACE kit, was employed allowing distinguishing PCR copies from original transcripts.

Article Title: Precision medicine for hepatocelluar carcinoma using molecular pattern diagnostics: results from a preclinical pilot study
Article Snippet: Massive analysis of cDNA ends MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described. .. The libraries were amplified by PCR, purified by SPRI beads (Agencourt AMPure XP; Beckman Coulter, Brea, CA, USA) and sequenced (NextSeq 500; Illumina Inc., San Diego, CA, USA).

Article Title: The NADPH organizers NoxO1 and p47phox are both mediators of diabetes-induced vascular dysfunction in mice
Article Snippet: The libraries were prepared using GenXPro “MACE kit v.2.0”. cDNA was produced using oligo dT priming. cDNA was sheared to an average size of 350 bps and fragments were ligated to “TrueQuant” (GenXPro property, contains unique molecule identifiers). .. MACE- tags were amplified with 10 PCR cycles and the libraries were sequenced on an Illumina NextSeq.

Next-Generation Sequencing:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC
Article Snippet: .. Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. A total of 152 million single 3'-end reads obtained from 24 mRNA sequencing libraries (total 8 conditions with 3 replicates) was initially filtered to eradicate adaptor sequences.

Construct:

Article Title: In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
Article Snippet: .. The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual. ..

Purification:

Article Title: Precision medicine for hepatocelluar carcinoma using molecular pattern diagnostics: results from a preclinical pilot study
Article Snippet: Massive analysis of cDNA ends MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described. .. In short, polyadenylated mRNA was extracted (Dynabeads mRNA Purification Kit; Life Technologies, Whaltham, MA, USA) from 5 μ g total RNA (large RNA fraction) and reverse transcribed with biotinylated oligo (dT) primers. cDNA was prepared and fragmented to an average size of 250 bp by sonification using a Bioruptor (Diagenode, Seraing, Belgium).

Produced:

Article Title: In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
Article Snippet: Preparation and sequencing of massive analysis of cDNA ends (MACE) libraries MACE libraries were produced for each replicate and treatment separately. .. The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual.

Article Title: The NADPH organizers NoxO1 and p47phox are both mediators of diabetes-induced vascular dysfunction in mice
Article Snippet: .. The libraries were prepared using GenXPro “MACE kit v.2.0”. cDNA was produced using oligo dT priming. cDNA was sheared to an average size of 350 bps and fragments were ligated to “TrueQuant” (GenXPro property, contains unique molecule identifiers). .. MACE- tags were amplified with 10 PCR cycles and the libraries were sequenced on an Illumina NextSeq.

Sequencing:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC
Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. A total of 152 million single 3'-end reads obtained from 24 mRNA sequencing libraries (total 8 conditions with 3 replicates) was initially filtered to eradicate adaptor sequences.

Article Title: In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
Article Snippet: Paragraph title: Preparation and sequencing of massive analysis of cDNA ends (MACE) libraries ... The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual.

Generated:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC
Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. Duplicate reads generated by PCR during library preparation were recognized by the TrueQuant technology included in the kit and also removed from the data set.

Expressing:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC
Article Snippet: Transcriptome profiling using MACE MACE is a 3'-end targeted tag-based reduced representation transcriptome profiling technique that can reliably quantify all poly-adenylated transcripts including those with low expression. .. Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described.

Polymerase Chain Reaction:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC
Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. Duplicate reads generated by PCR during library preparation were recognized by the TrueQuant technology included in the kit and also removed from the data set.

Article Title: In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
Article Snippet: The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual. .. In order to avoid bias during the PCR amplification steps included in the library preparation, GenXPro's PCR-bias-proof technology “TrueQuant,” implemented in the MACE kit, was employed allowing distinguishing PCR copies from original transcripts.

Article Title: Precision medicine for hepatocelluar carcinoma using molecular pattern diagnostics: results from a preclinical pilot study
Article Snippet: Massive analysis of cDNA ends MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described. .. The libraries were amplified by PCR, purified by SPRI beads (Agencourt AMPure XP; Beckman Coulter, Brea, CA, USA) and sequenced (NextSeq 500; Illumina Inc., San Diego, CA, USA).

Article Title: The NADPH organizers NoxO1 and p47phox are both mediators of diabetes-induced vascular dysfunction in mice
Article Snippet: The libraries were prepared using GenXPro “MACE kit v.2.0”. cDNA was produced using oligo dT priming. cDNA was sheared to an average size of 350 bps and fragments were ligated to “TrueQuant” (GenXPro property, contains unique molecule identifiers). .. MACE- tags were amplified with 10 PCR cycles and the libraries were sequenced on an Illumina NextSeq.

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    GenXPro mace kit
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mace kit/product/GenXPro
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mace kit - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    Image Search Results


    NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Journal: Cell Death & Disease

    Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC

    doi: 10.1038/cddis.2017.44

    Figure Lengend Snippet: NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described.

    Techniques: Next-Generation Sequencing, Quantitative RT-PCR, Sequencing, Expressing