rapid mace seq kit (GenXPro Inc)
Structured Review

Rapid Mace Seq Kit, supplied by GenXPro Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rapid mace seq kit/product/GenXPro Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "A small molecule cocktail for robust induction of blood-brain barrier properties"
Article Title: A small molecule cocktail for robust induction of blood-brain barrier properties
Journal: bioRxiv
doi: 10.1101/2023.02.09.527899

Figure Legend Snippet: a) Schematic drawing of the experimental setup. MACE-seq was performed on samples from brain-like ECs (in co-culture with PCs), with or without 48 h cARLA treatment. b) Principal component analysis (PCA) plot of control and cARLA samples. c) Venn-diagram showing the number of genes upregulated (log 2 FC ≥ 0.2 and FDR < 0.01), downregulated (log 2 FC ≥ -0.2 and FDR < 0.01) or not changed (|log 2 FC| < 0.2 and/or FDR ≥ 0.01) upon cARLA treatment. Log 2 FC: log 2 (fold change), FDR: false discovery rate. d) Mean-difference (MD) plot presenting the range and distribution of all transcripts (circles) regarding their mean expression and log 2 FC upon cARLA treatment. Key transcripts related to different aspects of BBB function are highlighted. e) Functional enrichment analysis of up- and downregulated gene sets upon cARLA treatment using g:Profiler. Gene Ontology Biological Process (GO:BP) terms are ranked based on their gene ratio (the fraction of differentially expressed genes in a given term) and are colored based on statistical significance (FDR). f) Representative staining of F-actin (grey), with or without cARLA treatment. Nuclei were stained with Hoechst (blue). Bar: 40 µm. g) Validation of the definitive endothelial nature of human stem cell-derived brain-like ECs. Normalized counts of key epithelial and endothelial transcripts from our dataset are plotted on a 3-segment y-axis covering multiple orders of magnitude. h) Scaled heatmap of 92 transcripts that are enriched in mouse brain ECs and are well expressed at the human BBB. Genes upregulated, downregulated or not changed upon cARLA treatment as well as genes not expressed (normalized counts ≤ 1) in this model are shown separately, in alphabetical order. Color coding represents row z-scores after Min-Max normalization, the darkest color corresponding to the highest expression in a given row.
Techniques Used: Co-Culture Assay, Expressing, Functional Assay, Staining, Derivative Assay

Figure Legend Snippet: Gene expressional changes after 48 h cARLA treatment were determined using MACE-seq profiling and are presented in panels a-i) by category. Mean ± SD, n =4. Red and blue color indicates up- and downregulation, respectively, upon cARLA treatment.
Techniques Used:
mace seq kit (GenXPro Inc)
Structured Review
Mace Seq Kit, supplied by GenXPro Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace seq kit/product/GenXPro Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
mace kit (GenXPro Inc)
Structured Review
Mace Kit, supplied by GenXPro Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace kit/product/GenXPro Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
mace seq kit (GenXPro Inc)
Structured Review
Mace Seq Kit, supplied by GenXPro Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace seq kit/product/GenXPro Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
mace seq kit (GenXPro Inc)
Structured Review
Mace Seq Kit, supplied by GenXPro Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace seq kit/product/GenXPro Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
3 mace kit (GenXPro Inc)
Structured Review
3 Mace Kit, supplied by GenXPro Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3 mace kit/product/GenXPro Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
mace seq kit (GenXPro Inc)
Structured Review
Mace Seq Kit, supplied by GenXPro Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace seq kit/product/GenXPro Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
cdna ends mace library preparation kit (GenXPro Inc)
Structured Review
Cdna Ends Mace Library Preparation Kit, supplied by GenXPro Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdna ends mace library preparation kit/product/GenXPro Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
mace seq kit v2 0 (GenXPro Inc)
Structured Review
Mace Seq Kit V2 0, supplied by GenXPro Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace seq kit v2 0/product/GenXPro Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
mace kit v 2 0 (GenXPro Inc)
Structured Review
Mace Kit V 2 0, supplied by GenXPro Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace kit v 2 0/product/GenXPro Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99