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GenXPro mace kit
<t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace kit/product/GenXPro
Average 87 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mace kit - by Bioz Stars, 2019-06
87/100 stars

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1) Product Images from "NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC"

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC

Journal: Cell Death & Disease

doi: 10.1038/cddis.2017.44

NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
Figure Legend Snippet: NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

Techniques Used: Next-Generation Sequencing, Quantitative RT-PCR, Sequencing, Expressing

Related Articles

Amplification:

Article Title: Precision medicine for hepatocelluar carcinoma using molecular pattern diagnostics: results from a preclinical pilot study
Article Snippet: MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described. .. MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described.

Polymerase Chain Reaction:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC
Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. A total of 152 million single 3'-end reads obtained from 24 mRNA sequencing libraries (total 8 conditions with 3 replicates) was initially filtered to eradicate adaptor sequences.

Article Title: Precision medicine for hepatocelluar carcinoma using molecular pattern diagnostics: results from a preclinical pilot study
Article Snippet: MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described. .. MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described.

Next-Generation Sequencing:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC
Article Snippet: MACE is a 3'-end targeted tag-based reduced representation transcriptome profiling technique that can reliably quantify all poly-adenylated transcripts including those with low expression. .. Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. A total of 152 million single 3'-end reads obtained from 24 mRNA sequencing libraries (total 8 conditions with 3 replicates) was initially filtered to eradicate adaptor sequences.

Purification:

Article Title: Precision medicine for hepatocelluar carcinoma using molecular pattern diagnostics: results from a preclinical pilot study
Article Snippet: MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described. .. MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described.

Produced:

Article Title: The NADPH organizers NoxO1 and p47phox are both mediators of diabetes-induced vascular dysfunction in mice
Article Snippet: 3 different pooled aliquots comprising different animals were analyzed for each group (WT and knockouts with and without diabetes). .. The libraries were prepared using GenXPro “MACE kit v.2.0”. cDNA was produced using oligo dT priming. cDNA was sheared to an average size of 350 bps and fragments were ligated to “TrueQuant” (GenXPro property, contains unique molecule identifiers). .. MACE- tags were amplified with 10 PCR cycles and the libraries were sequenced on an Illumina NextSeq.

High Throughput Screening Assay:

Article Title: A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea (Pisum sp.)
Article Snippet: MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. Briefly, cDNA from 5 μg of total RNA was randomly fragmented and biotinylated 3′ ends were captured after binding to a streptavidin matrix.

Generated:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC
Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. A total of 152 million single 3'-end reads obtained from 24 mRNA sequencing libraries (total 8 conditions with 3 replicates) was initially filtered to eradicate adaptor sequences.

Article Title: A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea (Pisum sp.)
Article Snippet: The integrity of the RNA samples was examined with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA). .. MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. Briefly, cDNA from 5 μg of total RNA was randomly fragmented and biotinylated 3′ ends were captured after binding to a streptavidin matrix.

Expressing:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC
Article Snippet: MACE is a 3'-end targeted tag-based reduced representation transcriptome profiling technique that can reliably quantify all poly-adenylated transcripts including those with low expression. .. Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described.

Sequencing:

Article Title: A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea (Pisum sp.)
Article Snippet: MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. Briefly, cDNA from 5 μg of total RNA was randomly fragmented and biotinylated 3′ ends were captured after binding to a streptavidin matrix.

Derivative Assay:

Article Title: A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea (Pisum sp.)
Article Snippet: MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. A library ready for high-throughput sequencing was prepared using TrueQuant adapters included in the kit.

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    GenXPro mace kit
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mace kit/product/GenXPro
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mace kit - by Bioz Stars, 2019-06
    87/100 stars
      Buy from Supplier

    Image Search Results


    NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Journal: Cell Death & Disease

    Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC

    doi: 10.1038/cddis.2017.44

    Figure Lengend Snippet: NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described.

    Techniques: Next-Generation Sequencing, Quantitative RT-PCR, Sequencing, Expressing