mace kit (GenXPro)

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GenXPro mace kit
Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace kit/product/GenXPro
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99

mace kit - by Bioz Stars, 2019-02

88/100 stars

Related Products / Commonly Used Together

truequant

PCR:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF- β receptor blocker galunisertib in HCC
Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. A total of 152 million single 3'-end reads obtained from 24 mRNA sequencing libraries (total 8 conditions with 3 replicates) was initially filtered to eradicate adaptor sequences.

Article Title: Precision medicine for hepatocelluar carcinoma using molecular pattern diagnostics: results from a preclinical pilot study
Article Snippet: MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described. .. MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described.

Article Title: In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
Article Snippet: The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual. .. These fragments are sequenced from their 5′-ends, thereby generating a single sequence from each transcript.

Derived:

Article Title: A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea ( Pisum sp.)
Article Snippet: MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. A library ready for high-throughput sequencing was prepared using TrueQuant adapters included in the kit.

Construct:

Article Title: In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
Article Snippet: MACE libraries were produced for each replicate and treatment separately. .. The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual. .. The technique analyzes 3′-biotinylated cDNA ends of 300–500 bp long, fragmented cDNA.

Produced:

Article Title: The NADPH organizers NoxO1 and p47phox are both mediators of diabetes-induced vascular dysfunction in mice
Article Snippet: 3 different pooled aliquots comprising different animals were analyzed for each group (WT and knockouts with and without diabetes). .. The libraries were prepared using GenXPro “MACE kit v.2.0”. cDNA was produced using oligo dT priming. cDNA was sheared to an average size of 350 bps and fragments were ligated to “TrueQuant” (GenXPro property, contains unique molecule identifiers). .. MACE- tags were amplified with 10 PCR cycles and the libraries were sequenced on an Illumina NextSeq 500 machine.

Article Title: In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
Article Snippet: MACE libraries were produced for each replicate and treatment separately. .. The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual.

Generated:

Article Title: A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea ( Pisum sp.)
Article Snippet: The integrity of the RNA samples was examined with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA). .. MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. Briefly, cDNA from 5 μg of total RNA was randomly fragmented and biotinylated 3′ ends were captured after binding to a streptavidin matrix.

Expressing:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF- β receptor blocker galunisertib in HCC
Article Snippet: MACE is a 3'-end targeted tag-based reduced representation transcriptome profiling technique that can reliably quantify all poly-adenylated transcripts including those with low expression. .. Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described.

Sequencing:

Article Title: A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea ( Pisum sp.)
Article Snippet: MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. Briefly, cDNA from 5 μg of total RNA was randomly fragmented and biotinylated 3′ ends were captured after binding to a streptavidin matrix.

Article Title: In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
Article Snippet: Paragraph title: Preparation and sequencing of massive analysis of cDNA ends (MACE) libraries ... The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual.