Structured Review

GenXPro mace kit
<t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace kit/product/GenXPro
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mace kit - by Bioz Stars, 2021-07
86/100 stars

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1) Product Images from "NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC"

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC

Journal: Cell Death & Disease

doi: 10.1038/cddis.2017.44

NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
Figure Legend Snippet: NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

Techniques Used: Next-Generation Sequencing, Quantitative RT-PCR, Sequencing, Expressing

Related Articles

Sequencing:

Article Title: MACE-Seq-based coding RNA and TrueQuant-based small RNA profile in breast cancer: tumor-suppressive miRNA-1275 identified as a novel marker
Article Snippet: .. Although analyses of MACE-sequencing and sRNA sequencing were done in Genxpro company, in Germany. ..

Generated:

Article Title: A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea (Pisum sp.)
Article Snippet: The integrity of the RNA samples was examined with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA). .. Massive analysis of cDNA ends (MACE) MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. Briefly, cDNA from 5 μg of total RNA was randomly fragmented and biotinylated 3′ ends were captured after binding to a streptavidin matrix.

Next-Generation Sequencing:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC
Article Snippet: Transcriptome profiling using MACE MACE is a 3'-end targeted tag-based reduced representation transcriptome profiling technique that can reliably quantify all poly-adenylated transcripts including those with low expression. .. Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. A total of 152 million single 3'-end reads obtained from 24 mRNA sequencing libraries (total 8 conditions with 3 replicates) was initially filtered to eradicate adaptor sequences.

Genome Wide:

Article Title: Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata
Article Snippet: This approach, based on polyacrylamide gel electrophoresis, may complement the existing methods for the detection of genome indels and is uniquely suited for the evaluation of CRISPR-induced events in combination with the direct assessment of a putatively associated phenotype. .. Obtainment of BcFLA1 BcFLA1 belongs to a list of candidate genes for the Pi deficiency-induced root hair elongation, which was obtained by a genome-wide expression profile analysis by massive analysis of cDNA ends (MACE; GenXPro GmbH, Frankfurt Main, Germany) [ ]. .. The BcFLA1 sequence obtained by MACE was extended by 5’ RNA ligase-mediated rapid amplification of cDNA ends (5’ RLM-RACE) using the ‘FirstChoice® RLM-RACE Kit’ (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer´s instructions.

Expressing:

Article Title: Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata
Article Snippet: This approach, based on polyacrylamide gel electrophoresis, may complement the existing methods for the detection of genome indels and is uniquely suited for the evaluation of CRISPR-induced events in combination with the direct assessment of a putatively associated phenotype. .. Obtainment of BcFLA1 BcFLA1 belongs to a list of candidate genes for the Pi deficiency-induced root hair elongation, which was obtained by a genome-wide expression profile analysis by massive analysis of cDNA ends (MACE; GenXPro GmbH, Frankfurt Main, Germany) [ ]. .. The BcFLA1 sequence obtained by MACE was extended by 5’ RNA ligase-mediated rapid amplification of cDNA ends (5’ RLM-RACE) using the ‘FirstChoice® RLM-RACE Kit’ (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer´s instructions.

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    GenXPro mace kit
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mace kit/product/GenXPro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mace kit - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    GenXPro genxpro s mace kit
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Genxpro S Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genxpro s mace kit/product/GenXPro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genxpro s mace kit - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    GenXPro mace seq kit v2 0
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Mace Seq Kit V2 0, supplied by GenXPro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mace seq kit v2 0/product/GenXPro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mace seq kit v2 0 - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Journal: Cell Death & Disease

    Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC

    doi: 10.1038/cddis.2017.44

    Figure Lengend Snippet: NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described.

    Techniques: Next-Generation Sequencing, Quantitative RT-PCR, Sequencing, Expressing