mace kit (GenXPro)

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GenXPro mace kit
Bioz Stars score: 88/100, Techniques, Protocol Conditions and more for mace kit, supplied by GenXPro. Data for mace kit gathered from related PubMed articles.
https://www.bioz.com/result/mace kit/product/GenXPro
Average 88 stars, based on 1 article reviews
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mace kit - by Bioz Product Reviews, 2018-12-17

88/100 stars

... of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided ...

Commonly Used Together

truequant,

Construct:

In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
MACE libraries were produced for each replicate and treatment separately. .. The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual. .. The technique analyzes 3′-biotinylated cDNA ends of 300–500 bp long, fragmented cDNA.

Produced:

The NADPH organizers NoxO1 and p47phox are both mediators of diabetes-induced vascular dysfunction in mice
3 different pooled aliquots comprising different animals were analyzed for each group (WT and knockouts with and without diabetes). .. The libraries were prepared using GenXPro “MACE kit v.2.0”. cDNA was produced using oligo dT priming. cDNA was sheared to an average size of 350 bps and fragments were ligated to “TrueQuant” (GenXPro property, contains unique molecule identifiers). .. MACE- tags were amplified with 10 PCR cycles and the libraries were sequenced on an Illumina NextSeq 500 machine.

Generated:

A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea ( Pisum sp.)
The integrity of the RNA samples was examined with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA). .. MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. Briefly, cDNA from 5 μg of total RNA was randomly fragmented and biotinylated 3′ ends were captured after binding to a streptavidin matrix.

Expressing:

NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF- β receptor blocker galunisertib in HCC
MACE is a 3'-end targeted tag-based reduced representation transcriptome profiling technique that can reliably quantify all poly-adenylated transcripts including those with low expression. .. Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described.

Sequencing:

In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
Paragraph title: Preparation and sequencing of massive analysis of cDNA ends (MACE) libraries ... The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual.