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GenXPro mace kit
<t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mace kit/product/GenXPro
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mace kit - by Bioz Stars, 2020-05
88/100 stars

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1) Product Images from "NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC"

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC

Journal: Cell Death & Disease

doi: 10.1038/cddis.2017.44

NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
Figure Legend Snippet: NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

Techniques Used: Next-Generation Sequencing, Quantitative RT-PCR, Sequencing, Expressing

Related Articles

Produced:

Article Title: The NADPH organizers NoxO1 and p47phox are both mediators of diabetes-induced vascular dysfunction in mice
Article Snippet: .. The libraries were prepared using GenXPro “MACE kit v.2.0”. cDNA was produced using oligo dT priming. cDNA was sheared to an average size of 350 bps and fragments were ligated to “TrueQuant” (GenXPro property, contains unique molecule identifiers). .. MACE- tags were amplified with 10 PCR cycles and the libraries were sequenced on an Illumina NextSeq.

Next-Generation Sequencing:

Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC
Article Snippet: .. Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. A total of 152 million single 3'-end reads obtained from 24 mRNA sequencing libraries (total 8 conditions with 3 replicates) was initially filtered to eradicate adaptor sequences.

Construct:

Article Title: In planta Identification of Putative Pathogenicity Factors from the Chickpea Pathogen Ascochyta rabiei by De novo Transcriptome Sequencing Using RNA-Seq and Massive Analysis of cDNA Ends
Article Snippet: .. The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual. ..

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    GenXPro mace kit
    <t>NGS</t> data in HLF cell line from <t>MACE</t> and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)
    Mace Kit, supplied by GenXPro, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mace kit/product/GenXPro
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mace kit - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

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    NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Journal: Cell Death & Disease

    Article Title: NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF-β receptor blocker galunisertib in HCC

    doi: 10.1038/cddis.2017.44

    Figure Lengend Snippet: NGS data in HLF cell line from MACE and validation by qRT-PCR. Three replicates were performed for each condition. ( a ) Clustered heat map of normalized sequence counts of the genes with the highest expression as determined by MACE. ( b ) Transcriptional response of selected potential biomarkers to both TGF- β 1 and galunisertib (gal). ( c ) Comparison of mRNA expression measurement results obtained with either qRT-PCR or MACE. For each gene, the change of expression level is calculated by fold change. ( d ) Expression of mRNA of target genes in comparison with their expression levels in control samples as measured by qRT-PCR. For each gene, the change of expression (−ΔΔCt) is calculated by – (delta Ct target–delta Ct control) and for MACE data the log 2 fold change is used)

    Article Snippet: Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described.

    Techniques: Next-Generation Sequencing, Quantitative RT-PCR, Sequencing, Expressing