mace kit (GenXPro)

4.9

GenXPro mace kit
The libraries were prepared using GenXPro “MACE kit v.2.0”. cDNA was produced using oligo dT priming. ..
https://www.bioz.com/result/mace kit/product/GenXPro
Average 4.9 stars, based on 1 article reviews
Price from $9.99 to $1999.99

mace kit - by Bioz Product Reviews, 2018-06-01

4.9/5 stars

The libraries were prepared using GenXPro “MACE kit v.2.0”. cDNA was produced using oligo dT priming. ..

Commonly Used Together

truequant,

PCR:

The NADPH organizers NoxO1 and p47phox are both mediators of diabetes-induced vascular dysfunction in mice
The libraries were prepared using GenXPro “MACE kit v.2.0”. cDNA was produced using oligo dT priming. cDNA was sheared to an average size of 350 bps and fragments were ligated to “TrueQuant” (GenXPro property, contains unique molecule identifiers). .. MACE- tags were amplified with 10 PCR cycles and the libraries were sequenced on an Illumina NextSeq.

NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF- β
Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. Duplicate reads generated by PCR during library preparation were recognized by the TrueQuant technology included in the kit and also removed from the data set.

In planta
The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual. .. In order to avoid bias during the PCR amplification steps included in the library preparation, GenXPro's PCR-bias-proof technology “TrueQuant,” implemented in the MACE kit, was employed allowing distinguishing PCR copies from original transcripts.

Precision medicine for hepatocelluar carcinoma using molecular pattern diagnostics: results from a preclinical pilot study
MACE libraries were prepared from the two peritumoral and the two tumor tissues using the MACE Kit (GenXPro GmbH, Frankfurt Germany) according to the supplier’s protocol as described. .. The libraries were amplified by PCR, purified by SPRI beads (Agencourt AMPure XP; Beckman Coulter, Brea, CA, USA) and sequenced (NextSeq 500; Illumina Inc., San Diego, CA, USA).

Derived:

A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea ( Pisum
MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. The library consisted of 50–700 bp-long fragments derived from the 3′-end of the cDNAs.

Binding:

A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea ( Pisum
MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. Briefly, cDNA from 5 μg of total RNA was randomly fragmented and biotinylated 3′ ends were captured after binding to a streptavidin matrix.

Construct:

In planta
MACE libraries were produced for each replicate and treatment separately. .. The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual. .. The technique analyzes 3′-biotinylated cDNA ends of 300–500 bp long, fragmented cDNA.

Produced:

The NADPH organizers NoxO1 and p47phox are both mediators of diabetes-induced vascular dysfunction in mice
3 different pooled aliquots comprising different animals were analyzed for each group (WT and knockouts with and without diabetes). .. The libraries were prepared using GenXPro “MACE kit v.2.0”. cDNA was produced using oligo dT priming. cDNA was sheared to an average size of 350 bps and fragments were ligated to “TrueQuant” (GenXPro property, contains unique molecule identifiers). .. MACE- tags were amplified with 10 PCR cycles and the libraries were sequenced on an Illumina NextSeq.

Generated:

A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea ( Pisum
The integrity of the RNA samples was examined with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA). .. MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. Briefly, cDNA from 5 μg of total RNA was randomly fragmented and biotinylated 3′ ends were captured after binding to a streptavidin matrix.

Expressing:

NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF- β
MACE is a 3'-end targeted tag-based reduced representation transcriptome profiling technique that can reliably quantify all poly-adenylated transcripts including those with low expression. .. Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described.

Sequencing:

A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea ( Pisum
MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ). .. A library ready for high-throughput sequencing was prepared using TrueQuant adapters included in the kit.

NGS-based transcriptome profiling reveals biomarkers for companion diagnostics of the TGF- β
Preparation and next-generation sequencing (NGS) of MACE libraries were performed using the MACE kit (GenXPro GmbH, Frankfurt, Germany) according to the manual provided with the kit and essentially as described. .. A total of 152 million single 3'-end reads obtained from 24 mRNA sequencing libraries (total 8 conditions with 3 replicates) was initially filtered to eradicate adaptor sequences.

In planta
Paragraph title: Preparation and sequencing of massive analysis of cDNA ends (MACE) libraries ... The libraries were constructed from ≈5 μg of total RNA using the “MACE-Kit” (GenXPro GmbH, Frankfurt am Main, Germany) according to the supplier's manual.

mace kit (GenXPro)

Commonly Used Together

Related Articles

PCR:

Derived:

Binding:

Construct:

Produced:

Generated:

Expressing:

Sequencing:

Purified:

Next Generation Sequencing:

Similar Products

About Bioz

Partner With Us

Our Platform

Contact Us

Careers