Journal: STAR Protocols

Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

doi: 10.1016/j.xpro.2026.104519

Figure Lengend Snippet: Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

Article Snippet: Note: If no FACS device is available, the sorting of naïve T cells can be performed using a naïve MACS Sort Kit from Miltenyi Biotec (130-104-453 ).

Techniques: Cell Culture, Dot Blot, Luminex

Journal: STAR Protocols

Article Title: Protocol for isolating stromal cells from lymphoid tissue for performing scRNA-seq

doi: 10.1016/j.xpro.2026.104501

Figure Lengend Snippet: Cell selection using automated magnetic cell sorting (A) Cells were stained with CD45-biotin and CD31-biotin and sorted using autoMACS® Pro Separator. (B) Number of cells before staining for autoMACS® separation (step 11), and number of cells recovered from positive selection (CD45 + and CD31 + cells) and negative selection (CD45 - and CD31 - cells) in step 23. Each dot represents combined numbers from inguinal, axillary and brachial lymph nodes from 6 mice. Colors indicate biological replicates. (C) Percentages of cells after separation compared to the pre-staining cell count performed in step 11. (D) Purity check of separated cells using flow cytometry and staining for CD45 and CD31. (E) Overlay plots of positive selection (blue) and negative selection (red). (F) Percentage of CD45 + , CD45 - and CD31 + cells recovered (∗∗∗∗ p value < 0.0001, ∗ p value < 0.05). (G) Final viability check of positive and negative selected cells acquired just before performing scRNA-sequencing analysis.

Article Snippet: autoMACS® Running buffer – MACS Separation Buffer , Miltenyi Biotec , Cat# 130-091-221.

Techniques: Selection, FACS, Staining, Cell Characterization, Flow Cytometry, Sequencing

Journal: Biomaterial Investigations in Dentistry

Article Title: Influence of repetitive diamond bur use on the trueness and surface roughness of subtractively manufactured PICN restorations

doi: 10.2340/biid.v13.46232

Figure Lengend Snippet: Bur degradation recorded during the manufacturing processes as the remaining bur life in the CAM programs of (A) PM7 diamond burs and (B) inLab MC X5 diamond burs. The changes in surface roughness of the diamond bur tips of (A) the PrograMill PM7 and (B) inLab MC X5 during repetitive milling cycles. Bur degradation recorded during the manufacturing processes as the remaining bur life in the CAM programs of (A) PM7 diamond burs and (B) inLab MC X5 diamond burs. The changes in surface roughness of the diamond bur tips of (A) the PrograMill PM7 and (B) inLab MC X5 during repetitive milling cycles.

Article Snippet: For the inLab Mac X5, a water-based lubricant mixture (Dentatec, Dentsply Sirona) was used, while the PrograMill PM7 utilized its proprietary cooling fluid (PrograMill Fluid, Ivoclar).

Techniques:

Journal: Biomaterial Investigations in Dentistry

Article Title: Influence of repetitive diamond bur use on the trueness and surface roughness of subtractively manufactured PICN restorations

doi: 10.2340/biid.v13.46232

Figure Lengend Snippet: Three-dimensional color deviation maps of diamond burs used in the (A) PrograMill PM7 and (B) inLab MC X5 across sequential milling intervals.

Article Snippet: For the inLab Mac X5, a water-based lubricant mixture (Dentatec, Dentsply Sirona) was used, while the PrograMill PM7 utilized its proprietary cooling fluid (PrograMill Fluid, Ivoclar).

Techniques:

Journal: Biomaterial Investigations in Dentistry

Article Title: Influence of repetitive diamond bur use on the trueness and surface roughness of subtractively manufactured PICN restorations

doi: 10.2340/biid.v13.46232

Figure Lengend Snippet: Scanning electron microscopy (SEM) micrographs of diamond burs before and after use (PrograMill PM7 after milling 74 crowns and inLab MC X5 after milling 47 crowns). Red arrows indicate the chipped or broken diamond particles; Blue arrows indicate the diamond particle loss; Yellow arrows and rectangles denote the wear pattern on the matrix.

Article Snippet: For the inLab Mac X5, a water-based lubricant mixture (Dentatec, Dentsply Sirona) was used, while the PrograMill PM7 utilized its proprietary cooling fluid (PrograMill Fluid, Ivoclar).

Techniques: Electron Microscopy

Journal: Biomaterial Investigations in Dentistry

Article Title: Influence of repetitive diamond bur use on the trueness and surface roughness of subtractively manufactured PICN restorations

doi: 10.2340/biid.v13.46232

Figure Lengend Snippet: The average positive and negative deviations (unit: µm) of the overall inner and outer surfaces machined by (A) the PrograMill PM7 and (B) the inLab MC X5 systems.

Article Snippet: For the inLab Mac X5, a water-based lubricant mixture (Dentatec, Dentsply Sirona) was used, while the PrograMill PM7 utilized its proprietary cooling fluid (PrograMill Fluid, Ivoclar).

Techniques:

Journal: Biomaterial Investigations in Dentistry

Article Title: Influence of repetitive diamond bur use on the trueness and surface roughness of subtractively manufactured PICN restorations

doi: 10.2340/biid.v13.46232

Figure Lengend Snippet: The average root mean square (RMS) ± standard deviation (SD) (unit: µm) of various anatomical regions of PICN crowns manufactured from the inLab MC X5.

Article Snippet: For the inLab Mac X5, a water-based lubricant mixture (Dentatec, Dentsply Sirona) was used, while the PrograMill PM7 utilized its proprietary cooling fluid (PrograMill Fluid, Ivoclar).

Techniques: Standard Deviation

Journal: Biomaterial Investigations in Dentistry

Article Title: Influence of repetitive diamond bur use on the trueness and surface roughness of subtractively manufactured PICN restorations

doi: 10.2340/biid.v13.46232

Figure Lengend Snippet: Sequential 3D color deviation maps (unit: mm) of PICN crowns milled with (A) the PrograMill PM7 and (B) the inLab MC X5.

Article Snippet: For the inLab Mac X5, a water-based lubricant mixture (Dentatec, Dentsply Sirona) was used, while the PrograMill PM7 utilized its proprietary cooling fluid (PrograMill Fluid, Ivoclar).

Techniques:

Journal: Biomaterial Investigations in Dentistry

Article Title: Influence of repetitive diamond bur use on the trueness and surface roughness of subtractively manufactured PICN restorations

doi: 10.2340/biid.v13.46232

Figure Lengend Snippet: Average surface roughness (Ra, Sa, Sq) ± standard deviation (SD) (unit: µm) of PICN crowns manufactured from (A) the PrograMill PM7 and (B) the inLab MC X5.

Article Snippet: For the inLab Mac X5, a water-based lubricant mixture (Dentatec, Dentsply Sirona) was used, while the PrograMill PM7 utilized its proprietary cooling fluid (PrograMill Fluid, Ivoclar).

Techniques: Standard Deviation