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anti cnf1 antibody  (Hycult Biotech)


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    Structured Review

    Hycult Biotech anti cnf1 antibody
    Anti Cnf1 Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mab+ng8/CNF1%2C+mAb+NG8/pm29738516-111-12-16
    Average 90 stars, based on 1 article reviews
    anti cnf1 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Cytotoxic Necrotizing Factor Type 1-Neutralizing Monoclonal Antibody NG8 Recognizes Three Amino Acids in a C-Terminal Region of the Toxin and Reduces Toxin Binding to HEp-2 Cells
    Article Snippet: .. Purified toxins (0.33 g/well) were incubated for 2 h at 37°C with 5 g of purified MAb NG8 (IgG2a; Hycult, The Netherlands), with 1:6 dilutions of MAb NG8, or with one of the following negative control antibodies: 5 g of MAb 11E10, an anti-Shiga toxin type 2 A-subunit MAb (IgG1; Hycult); or 1 g of nonneutralizing anti-CNF1/CNF2 MAb GC2 (IgG2a hybridoma culture supernatant prepared in our laboratory). ..

    Article Title: Cytotoxic Necrotizing Factor Type 1-Neutralizing Monoclonal Antibody NG8 Recognizes Three Amino Acids in a C-Terminal Region of the Toxin and Reduces Toxin Binding to HEp-2 Cells
    Article Snippet: Cells were incubated for 72 h at 37°C in the presence of 5% CO 2 , fixed, and stained with Hema-3 (Fisher Scientific), and the degree of multinucleation was determined by light microscopy. .. Purified toxins (0.33 μg/well) were incubated for 2 h at 37°C with 5 μg of purified MAb NG8 (IgG2a; Hycult, The Netherlands), with 1:6 dilutions of MAb NG8, or with one of the following negative control antibodies: 5 μg of MAb 11E10, an anti-Shiga toxin type 2 A-subunit MAb (IgG1; Hycult); or 1 μg of nonneutralizing anti-CNF1/CNF2 MAb GC2 (IgG2a hybridoma culture supernatant prepared in our laboratory). ..

    Incubation:

    Article Title: Cytotoxic Necrotizing Factor Type 1-Neutralizing Monoclonal Antibody NG8 Recognizes Three Amino Acids in a C-Terminal Region of the Toxin and Reduces Toxin Binding to HEp-2 Cells
    Article Snippet: .. Purified toxins (0.33 g/well) were incubated for 2 h at 37°C with 5 g of purified MAb NG8 (IgG2a; Hycult, The Netherlands), with 1:6 dilutions of MAb NG8, or with one of the following negative control antibodies: 5 g of MAb 11E10, an anti-Shiga toxin type 2 A-subunit MAb (IgG1; Hycult); or 1 g of nonneutralizing anti-CNF1/CNF2 MAb GC2 (IgG2a hybridoma culture supernatant prepared in our laboratory). ..

    Article Title: Cytotoxic Necrotizing Factor Type 1-Neutralizing Monoclonal Antibody NG8 Recognizes Three Amino Acids in a C-Terminal Region of the Toxin and Reduces Toxin Binding to HEp-2 Cells
    Article Snippet: Cells were incubated for 72 h at 37°C in the presence of 5% CO 2 , fixed, and stained with Hema-3 (Fisher Scientific), and the degree of multinucleation was determined by light microscopy. .. Purified toxins (0.33 μg/well) were incubated for 2 h at 37°C with 5 μg of purified MAb NG8 (IgG2a; Hycult, The Netherlands), with 1:6 dilutions of MAb NG8, or with one of the following negative control antibodies: 5 μg of MAb 11E10, an anti-Shiga toxin type 2 A-subunit MAb (IgG1; Hycult); or 1 μg of nonneutralizing anti-CNF1/CNF2 MAb GC2 (IgG2a hybridoma culture supernatant prepared in our laboratory). ..

    Negative Control:

    Article Title: Cytotoxic Necrotizing Factor Type 1-Neutralizing Monoclonal Antibody NG8 Recognizes Three Amino Acids in a C-Terminal Region of the Toxin and Reduces Toxin Binding to HEp-2 Cells
    Article Snippet: .. Purified toxins (0.33 g/well) were incubated for 2 h at 37°C with 5 g of purified MAb NG8 (IgG2a; Hycult, The Netherlands), with 1:6 dilutions of MAb NG8, or with one of the following negative control antibodies: 5 g of MAb 11E10, an anti-Shiga toxin type 2 A-subunit MAb (IgG1; Hycult); or 1 g of nonneutralizing anti-CNF1/CNF2 MAb GC2 (IgG2a hybridoma culture supernatant prepared in our laboratory). ..

    Article Title: Cytotoxic Necrotizing Factor Type 1-Neutralizing Monoclonal Antibody NG8 Recognizes Three Amino Acids in a C-Terminal Region of the Toxin and Reduces Toxin Binding to HEp-2 Cells
    Article Snippet: Cells were incubated for 72 h at 37°C in the presence of 5% CO 2 , fixed, and stained with Hema-3 (Fisher Scientific), and the degree of multinucleation was determined by light microscopy. .. Purified toxins (0.33 μg/well) were incubated for 2 h at 37°C with 5 μg of purified MAb NG8 (IgG2a; Hycult, The Netherlands), with 1:6 dilutions of MAb NG8, or with one of the following negative control antibodies: 5 μg of MAb 11E10, an anti-Shiga toxin type 2 A-subunit MAb (IgG1; Hycult); or 1 μg of nonneutralizing anti-CNF1/CNF2 MAb GC2 (IgG2a hybridoma culture supernatant prepared in our laboratory). ..



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