Journal: Journal of chemical neuroanatomy

Article Title: Modeling Cerebellar Limb Dysmetria and Impaired Spatial Memory in Rats Using Lamivudine: A preliminary study

doi: 10.1016/j.jchemneu.2020.101838

Figure Lengend Snippet: Distribution of CD68 positive microglial cells. Immunohistochemistry of CD 68 in the cerebellum x1000, **p<0.01 against control.

Article Snippet: Sections were incubated with primary prediluted GFAP (IR524) Dako, and CD68 antibody (M0814) Dako, at 1:100 dilution for 45 minutes each, and sections washed with PBS for 3 minutes.

Techniques: Immunohistochemistry

Journal: Cancers

Article Title: Together Intra-Tumor Hypoxia and Macrophagic Immunity Are Driven Worst Outcome in Pediatric High-Grade Osteosarcomas

doi: 10.3390/cancers14061482

Figure Lengend Snippet: Hypoxic and macrophagic biomarkers studied by immunohistochemistry and their clinical correlations.

Article Snippet: We used the following primary antibodies: CD68 (clone KP1, M0814, dilution: 1/1000 e , Dako, Glostrup, Denmark), CD163 (clone 10D6, MS-1103-S, dilution: 1/200 e , Neomarkers, Portsmouth, NH, USA), HIF-1α (ab8366, dilution: 1/1000 e , Abcam, Cambridge, UK), pmTor (clone 49F9, dilution: 1/100 e , Cell Signaling, Danvers, MA, USA), pS6-RP (clone54D2, Cell Signaling, dilution: 1/100 e ) and CAIX (clone MRQ-54, dilution: 1/100 e , Cell Marque, Rocklin, CA, USA).

Techniques: Immunohistochemistry, Expressing

Journal: Cancers

Article Title: Together Intra-Tumor Hypoxia and Macrophagic Immunity Are Driven Worst Outcome in Pediatric High-Grade Osteosarcomas

doi: 10.3390/cancers14061482

Figure Lengend Snippet: Examples of immunohistochemical staining with hypoxic markers ( upper panels : phospho-mTor (pmTor), pS6-RP, HIF-1α and CAIX and their paired controls) and macrophagic markers ( bottom panel : CD68 and CD163 with their paired controls). Scalebar = 20 µm, black arrows show examples of intra-tumor giant cells.

Article Snippet: We used the following primary antibodies: CD68 (clone KP1, M0814, dilution: 1/1000 e , Dako, Glostrup, Denmark), CD163 (clone 10D6, MS-1103-S, dilution: 1/200 e , Neomarkers, Portsmouth, NH, USA), HIF-1α (ab8366, dilution: 1/1000 e , Abcam, Cambridge, UK), pmTor (clone 49F9, dilution: 1/100 e , Cell Signaling, Danvers, MA, USA), pS6-RP (clone54D2, Cell Signaling, dilution: 1/100 e ) and CAIX (clone MRQ-54, dilution: 1/100 e , Cell Marque, Rocklin, CA, USA).

Techniques: Immunohistochemical staining, Staining

Journal: Cancers

Article Title: Together Intra-Tumor Hypoxia and Macrophagic Immunity Are Driven Worst Outcome in Pediatric High-Grade Osteosarcomas

doi: 10.3390/cancers14061482

Figure Lengend Snippet: Kaplan–Meier significant correlations with immunohistological analyses of hypoxic and macrophagic biomarkers. On the upper rows, Kaplan–Meier estimates curves of OS and EFS according to the expression of pS6-RP (pS6) (( A , B ), respectively), HIF-1α (HIF) ( C , D ), respectively) and carbonic anhydrase IX (CAIX) (( E , F ), respectively). On the two last rows, Kaplan–Meier estimates of OS ( G ) and EFS ( H ) by the presence or absence of intra-tumor giant cells (GC). We stratified the population with three biomarkers associating CD68, CD163 and GC presence to a worse OS and EFS (( I , J ) curves). Finally, OS and EFS distributions based on the expression of CD68 based on the cutpoint of 17.5% expression (( K , L ) graphs, respectively). p values are reported on the right corner of the graphs, in bold when p < 0.05.

Article Snippet: We used the following primary antibodies: CD68 (clone KP1, M0814, dilution: 1/1000 e , Dako, Glostrup, Denmark), CD163 (clone 10D6, MS-1103-S, dilution: 1/200 e , Neomarkers, Portsmouth, NH, USA), HIF-1α (ab8366, dilution: 1/1000 e , Abcam, Cambridge, UK), pmTor (clone 49F9, dilution: 1/100 e , Cell Signaling, Danvers, MA, USA), pS6-RP (clone54D2, Cell Signaling, dilution: 1/100 e ) and CAIX (clone MRQ-54, dilution: 1/100 e , Cell Marque, Rocklin, CA, USA).

Techniques: Expressing