m mulv reverse transcriptase  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Name:
    M MuLV Reverse Transcriptase
    Description:
    M MuLV Reverse Transcriptase 50 000 units
    Catalog Number:
    m0253l
    Price:
    282
    Size:
    50 000 units
    Category:
    Reverse Transcriptases
    Buy from Supplier


    Structured Review

    New England Biolabs m mulv reverse transcriptase
    M MuLV Reverse Transcriptase
    M MuLV Reverse Transcriptase 50 000 units
    https://www.bioz.com/result/m mulv reverse transcriptase/product/New England Biolabs
    Average 90 stars, based on 346 article reviews
    Price from $9.99 to $1999.99
    m mulv reverse transcriptase - by Bioz Stars, 2020-01
    90/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: cAMP microdomains and L-type Ca2+ channel regulation in guinea-pig ventricular myocytes
    Article Snippet: PCR was carried out using 0.2 μg of total RNA and M-MuLV reverse transcriptase (New England Biolabs). .. As the guinea pig EP receptors have not been cloned, previously published primer sequences for mouse receptors were used ( ).

    Amplification:

    Article Title: cAMP microdomains and L-type Ca2+ channel regulation in guinea-pig ventricular myocytes
    Article Snippet: PCR was carried out using 0.2 μg of total RNA and M-MuLV reverse transcriptase (New England Biolabs). .. The resulting cDNA was amplified by 35 polymerase chain reaction (PCR) cycles using an annealing temperature of 60°C with primer sets specific for each EP receptor.

    Article Title: Physiological and Molecular Biological Characterization of Intracellular Carbonic Anhydrase from the Marine Diatom Phaeodactylum tricornutum 1
    Article Snippet: The cells were frozen immediately, disrupted in liquid N2 , and total RNA was extracted as described by . cDNA was synthesized with M-MuLV Reverse Transcriptase (New England Bio Labs Inc., Beverly, MA) and oligo (dT)15 primer. .. A partial nucleotide sequence of CA cDNA was amplified by PCR, on the assumption that P. tricornutum CA is homologous to β-CAs.

    Article Title: Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis
    Article Snippet: One microgram of fiber cell RNA and at least 200 ng of epithelia RNA were treated with amplification grade DNase I (Thermo Fisher Scientific), for each sample, to remove genomic DNA. .. RNA was converted to cDNA using M-MuLV Reverse Transcriptase and murine RNase inhibitor (New England BioLabs).

    Synthesized:

    Article Title: Physiological and Molecular Biological Characterization of Intracellular Carbonic Anhydrase from the Marine Diatom Phaeodactylum tricornutum 1
    Article Snippet: .. The cells were frozen immediately, disrupted in liquid N2 , and total RNA was extracted as described by . cDNA was synthesized with M-MuLV Reverse Transcriptase (New England Bio Labs Inc., Beverly, MA) and oligo (dT)15 primer. .. Degenerate primers were designed according to the amino acid sequence of the N-terminal end of the purified CA (PtCAF1: 5′-CGC GGA TCC GAY ATI ACI GAR ATH TTY GAY GG-3′) and according to two published nucleotide sequences of β-CAs from Coccomyxa sp. ( ) and P. purpreum ( ; PtCAR1: 5′-CCG GAA TTC CCA TNG CNK CYT GIA CHA T-3′), which corresponded to the sequences of DITEIFDG and IVQ(A/D) AW(A/D), respectively.

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: .. Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated. .. The library fragments were purified, for a 150–200 bp size range, with AMpure XP purification kit (Beckman Coulter, MA, USA).

    Article Title: Altered expression of base excision repair genes in response to high glucose-induced oxidative stress in HepG2 hepatocytes
    Article Snippet: .. Quantitative real-time PCR Total RNA was extracted from HepG2 cells using Trizol (Invitrogen), and cDNA was synthesized from 2 μg total RNA using M-MuLV reverse transcriptase (New England Biolabs, Beverly, MA). .. Real-time PCR was performed in a Lightcycler (Applied Biosystems, Foster City, CA) using the SYBR Green Master Mix Kit.

    Quantitative RT-PCR:

    Article Title: C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development
    Article Snippet: Paragraph title: C. elegans qRT-PCR Experiments ... Animals were washed in M9 buffer, and total RNA was isolated using TRIzol (Invitrogen) followed by DNase I treatment and cleanup using QIAGEN RNeasy columns. cDNA was generated from 1 μg RNA using random primer and M-MuLV reverse transcriptase (New England Biolabs). qPCR was performed in technical duplicates per gene per condition using the Applied Biosystems StepOnePlus Real-Time PCR System and the Fast SYBR Green Master Mix (Thermo Fisher Scientific).

    Article Title: The decay of Redox-stress Response Capacity is a substantive characteristic of aging: Revising the redox theory of aging
    Article Snippet: .. RNA samples were then reverse-transcribed using M-MuLV reverse transcriptase (NEB), and mRNA levels were measured by performing RT-qPCR on a 7500 Real-Time PCR System (Applied Biosystems). ..

    Article Title: The Myeloid Transcription Factor KLF2 Regulates the Host Response to Polymicrobial Infection and Endotoxic Shock
    Article Snippet: Paragraph title: Real-time Quantitative RT-PCR ... Total RNA was extracted from primary macrophages, neutrophils or RAW 264.7 cells following indicated treatment using TRIzol® Reagent (Invitrogen) and 2 µg of total RNA was reverse transcribed using M-MuLV reverse transcriptase (New England Biolabs Inc.) and mixture of random and oligo-dT primers.

    Article Title: TET-mediated epimutagenesis of the Arabidopsis thaliana methylome
    Article Snippet: For RT-qPCR, RNA was further treated with TURBO™ DNase (Thermo Scientific) according to the manufacturer's instructions. .. One microgram of RNA was subsequently reverse transcribed with M-MuLV reverse transcriptase according to the manufacturer's instructions (NEB).

    Article Title: Two Theobroma cacao genotypes with contrasting pathogen tolerance show aberrant transcriptional and ROS responses after salicylic acid treatment
    Article Snippet: .. One microgram of RNA from each sample was reverse transcribed by M MuLV Reverse Transcriptase (New England Biolabs) using oligo-(dT)15 as a primer to generate cDNA. qRT-PCR was performed in 10 µl reactions, consisting of 5 µl of SYBR Green PCR Master Mix (Takara), 0.2 µl of Rox, and 0.4 µl of each primer, diluted to 5 µM, and 4 µl of cDNA. .. Each reaction was performed in technical duplicate using the Applied Biosystem Step One Plus Realtime PCR System (Roche) with the following programme: 15min at 94°C, 40 cycles of 15 s at 94°C, 20 s at 60°C and 40 s at 72°C.

    SYBR Green Assay:

    Article Title: C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development
    Article Snippet: .. Animals were washed in M9 buffer, and total RNA was isolated using TRIzol (Invitrogen) followed by DNase I treatment and cleanup using QIAGEN RNeasy columns. cDNA was generated from 1 μg RNA using random primer and M-MuLV reverse transcriptase (New England Biolabs). qPCR was performed in technical duplicates per gene per condition using the Applied Biosystems StepOnePlus Real-Time PCR System and the Fast SYBR Green Master Mix (Thermo Fisher Scientific). .. Relative transcript abundance was determined using the ΔΔCt method and normalized to averaged ama-1 mRNA expression levels.

    Article Title: The Myeloid Transcription Factor KLF2 Regulates the Host Response to Polymicrobial Infection and Endotoxic Shock
    Article Snippet: Total RNA was extracted from primary macrophages, neutrophils or RAW 264.7 cells following indicated treatment using TRIzol® Reagent (Invitrogen) and 2 µg of total RNA was reverse transcribed using M-MuLV reverse transcriptase (New England Biolabs Inc.) and mixture of random and oligo-dT primers. .. Real-Time PCR was performed using Universal SYBR Green PCR Master Mix on Applied Biosystems Step One Real-Time PCR System (Applied Biosystems) using gene specific primers.

    Article Title: TET-mediated epimutagenesis of the Arabidopsis thaliana methylome
    Article Snippet: One microgram of RNA was subsequently reverse transcribed with M-MuLV reverse transcriptase according to the manufacturer's instructions (NEB). .. RT-qPCR was used to analyze cDNA populations using PP2AA-3 (AT1G13320) as an endogenous control, and was performed on a Roche LIghtCycler 480 instrument using SYBR Green detection chemistry.

    Article Title: Inhibition of mitochondrial respiration under hypoxia and increased antioxidant activity after reoxygenation of Tribolium castaneum
    Article Snippet: .. To prepare qRT-PCR templates, we used 2 μg of RNA to synthesize cDNA with random primers and M-Mulv reverse transcriptase (New England Biolabs, Beverly, MA, USA). qRT-PCR reactions were conducted using Power SYBR Green PCR Master Mix and run on a real-time thermal cycler (Bio-Rad, USA). ..

    Article Title: Two Theobroma cacao genotypes with contrasting pathogen tolerance show aberrant transcriptional and ROS responses after salicylic acid treatment
    Article Snippet: .. One microgram of RNA from each sample was reverse transcribed by M MuLV Reverse Transcriptase (New England Biolabs) using oligo-(dT)15 as a primer to generate cDNA. qRT-PCR was performed in 10 µl reactions, consisting of 5 µl of SYBR Green PCR Master Mix (Takara), 0.2 µl of Rox, and 0.4 µl of each primer, diluted to 5 µM, and 4 µl of cDNA. .. Each reaction was performed in technical duplicate using the Applied Biosystem Step One Plus Realtime PCR System (Roche) with the following programme: 15min at 94°C, 40 cycles of 15 s at 94°C, 20 s at 60°C and 40 s at 72°C.

    Article Title: Altered expression of base excision repair genes in response to high glucose-induced oxidative stress in HepG2 hepatocytes
    Article Snippet: Quantitative real-time PCR Total RNA was extracted from HepG2 cells using Trizol (Invitrogen), and cDNA was synthesized from 2 μg total RNA using M-MuLV reverse transcriptase (New England Biolabs, Beverly, MA). .. Real-time PCR was performed in a Lightcycler (Applied Biosystems, Foster City, CA) using the SYBR Green Master Mix Kit.

    Microarray:

    Article Title: Two Theobroma cacao genotypes with contrasting pathogen tolerance show aberrant transcriptional and ROS responses after salicylic acid treatment
    Article Snippet: qRT-PCR measurement of selected genes qRT-PCR analyses were performed on the same RNA samples produced for the microarray experiment. .. One microgram of RNA from each sample was reverse transcribed by M MuLV Reverse Transcriptase (New England Biolabs) using oligo-(dT)15 as a primer to generate cDNA. qRT-PCR was performed in 10 µl reactions, consisting of 5 µl of SYBR Green PCR Master Mix (Takara), 0.2 µl of Rox, and 0.4 µl of each primer, diluted to 5 µM, and 4 µl of cDNA.

    Incubation:

    Article Title: Identification of Telomerase RNAs from Filamentous Fungi Reveals Conservation with Vertebrates and Yeasts
    Article Snippet: .. Buffer, 40 U of RNase inhibitor (Ambion) and 200 U of M-MuLV Reverse Transcriptase (New England BioLabs) was added and incubated at 42°C for one hour, followed by incubation at 90°C for 10 minutes to inactivate the enzymes. .. PCR Conditions Each tube contained 1 µL of cDNA (either RNased cDNA from reverse transcription, or cDNA from reverse transcription) or 150–300 ng of isolated A. oryzae DNA, in addition to 1.25 µM forward primer, 1.25 µM reverse primer , 17 µl of nuclease free water, and 20 µl JumpStart REDTaq ReadyMix PCR Reaction Mix (Sigma).

    Expressing:

    Article Title: C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development
    Article Snippet: Animals were washed in M9 buffer, and total RNA was isolated using TRIzol (Invitrogen) followed by DNase I treatment and cleanup using QIAGEN RNeasy columns. cDNA was generated from 1 μg RNA using random primer and M-MuLV reverse transcriptase (New England Biolabs). qPCR was performed in technical duplicates per gene per condition using the Applied Biosystems StepOnePlus Real-Time PCR System and the Fast SYBR Green Master Mix (Thermo Fisher Scientific). .. Relative transcript abundance was determined using the ΔΔCt method and normalized to averaged ama-1 mRNA expression levels.

    Article Title: Inhibition of mitochondrial respiration under hypoxia and increased antioxidant activity after reoxygenation of Tribolium castaneum
    Article Snippet: A value of p ≤ 0.05, FDR ≤ 0.001 and fold change ≥1.5 provided significance thresholds for gene expression differences. .. To prepare qRT-PCR templates, we used 2 μg of RNA to synthesize cDNA with random primers and M-Mulv reverse transcriptase (New England Biolabs, Beverly, MA, USA). qRT-PCR reactions were conducted using Power SYBR Green PCR Master Mix and run on a real-time thermal cycler (Bio-Rad, USA).

    Transformation Assay:

    Article Title: Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons
    Article Snippet: .. Isolated mRNA was immediately transformed into cDNA using M-MuLV Reverse Transcriptase (New England Biolabs (NEB), M0253L; other NEB reagents: S1330S, M0314S). ..

    Cell Culture:

    Article Title: Noncanonical Wnt signaling plays an important role in modulating canonical Wnt-regulated stemness, proliferation and terminal differentiation of hepatic progenitors
    Article Snippet: For the cultured cells, subconfluent iHPx cells were treated with different conditions for varied duration and lysed in TRIZOL Reagent. .. Total RNA was extracted according to the manufacturer's instructions and subjected to reverse transcription reactions with hexamer and M-MuLV reverse transcriptase (New England Biolabs, Ipswich, MA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: eIF4A inhibition prevents the onset of cytokine-induced muscle wasting by blocking the STAT3 and iNOS pathways
    Article Snippet: .. Reverse Transcription PCR (RT-PCR) and Quantitative PCR (qPCR) Total RNA was reverse transcribed with the M-MuLV Reverse Transcriptase (New England Biolabs). .. Resulting cDNA was diluted 1/20 and quantified using Sso Fast EvaGreen Supermix (Biorad) as described previously .

    Generated:

    Article Title: C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development
    Article Snippet: .. Animals were washed in M9 buffer, and total RNA was isolated using TRIzol (Invitrogen) followed by DNase I treatment and cleanup using QIAGEN RNeasy columns. cDNA was generated from 1 μg RNA using random primer and M-MuLV reverse transcriptase (New England Biolabs). qPCR was performed in technical duplicates per gene per condition using the Applied Biosystems StepOnePlus Real-Time PCR System and the Fast SYBR Green Master Mix (Thermo Fisher Scientific). .. Relative transcript abundance was determined using the ΔΔCt method and normalized to averaged ama-1 mRNA expression levels.

    Sequencing:

    Article Title: Physiological and Molecular Biological Characterization of Intracellular Carbonic Anhydrase from the Marine Diatom Phaeodactylum tricornutum 1
    Article Snippet: The cells were frozen immediately, disrupted in liquid N2 , and total RNA was extracted as described by . cDNA was synthesized with M-MuLV Reverse Transcriptase (New England Bio Labs Inc., Beverly, MA) and oligo (dT)15 primer. .. Degenerate primers were designed according to the amino acid sequence of the N-terminal end of the purified CA (PtCAF1: 5′-CGC GGA TCC GAY ATI ACI GAR ATH TTY GAY GG-3′) and according to two published nucleotide sequences of β-CAs from Coccomyxa sp. ( ) and P. purpreum ( ; PtCAR1: 5′-CCG GAA TTC CCA TNG CNK CYT GIA CHA T-3′), which corresponded to the sequences of DITEIFDG and IVQ(A/D) AW(A/D), respectively.

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: The NEBNext Ultra RNA Library Prep Kit for Illumina sequencing (NEB, MA, USA) following the protocol as provided by the manufacturer was used for library preparation, index sequences were added to trace back the sequences to each sample. .. Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated.

    Sonication:

    Article Title: TET-mediated epimutagenesis of the Arabidopsis thaliana methylome
    Article Snippet: DNA extraction was carried out on all samples using the DNeasy Plant Mini Kit (Qiagen), and the DNA was sheered to ~200 bp by sonication. .. One microgram of RNA was subsequently reverse transcribed with M-MuLV reverse transcriptase according to the manufacturer's instructions (NEB).

    DNA Extraction:

    Article Title: TET-mediated epimutagenesis of the Arabidopsis thaliana methylome
    Article Snippet: DNA extraction was carried out on all samples using the DNeasy Plant Mini Kit (Qiagen), and the DNA was sheered to ~200 bp by sonication. .. One microgram of RNA was subsequently reverse transcribed with M-MuLV reverse transcriptase according to the manufacturer's instructions (NEB).

    Molecular Cloning:

    Article Title: Physiological and Molecular Biological Characterization of Intracellular Carbonic Anhydrase from the Marine Diatom Phaeodactylum tricornutum 1
    Article Snippet: Paragraph title: Extraction of Total RNA and Molecular Cloning of CA cDNA ... The cells were frozen immediately, disrupted in liquid N2 , and total RNA was extracted as described by . cDNA was synthesized with M-MuLV Reverse Transcriptase (New England Bio Labs Inc., Beverly, MA) and oligo (dT)15 primer.

    RNA Sequencing Assay:

    Article Title: Inhibition of mitochondrial respiration under hypoxia and increased antioxidant activity after reoxygenation of Tribolium castaneum
    Article Snippet: The RNA samples were the same ones used for RNA-seq mentioned previously. .. To prepare qRT-PCR templates, we used 2 μg of RNA to synthesize cDNA with random primers and M-Mulv reverse transcriptase (New England Biolabs, Beverly, MA, USA). qRT-PCR reactions were conducted using Power SYBR Green PCR Master Mix and run on a real-time thermal cycler (Bio-Rad, USA).

    Article Title: Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis
    Article Snippet: Paragraph title: Validation of RNA-Seq Data by Real Time PCR (qPCR) ... RNA was converted to cDNA using M-MuLV Reverse Transcriptase and murine RNase inhibitor (New England BioLabs).

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: Paragraph title: RNA sequencing ... Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated.

    Magnetic Beads:

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: The mRNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation using NEBNext First Strand Synthesis Reaction Buffer (5x). .. Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated.

    Isolation:

    Article Title: cAMP microdomains and L-type Ca2+ channel regulation in guinea-pig ventricular myocytes
    Article Snippet: RNA was prepared from isolated ventricular myocytes using Tripure RNA isolation reagent (Roche). .. PCR was carried out using 0.2 μg of total RNA and M-MuLV reverse transcriptase (New England Biolabs).

    Article Title: C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development
    Article Snippet: .. Animals were washed in M9 buffer, and total RNA was isolated using TRIzol (Invitrogen) followed by DNase I treatment and cleanup using QIAGEN RNeasy columns. cDNA was generated from 1 μg RNA using random primer and M-MuLV reverse transcriptase (New England Biolabs). qPCR was performed in technical duplicates per gene per condition using the Applied Biosystems StepOnePlus Real-Time PCR System and the Fast SYBR Green Master Mix (Thermo Fisher Scientific). .. Relative transcript abundance was determined using the ΔΔCt method and normalized to averaged ama-1 mRNA expression levels.

    Article Title: Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons
    Article Snippet: .. Isolated mRNA was immediately transformed into cDNA using M-MuLV Reverse Transcriptase (New England Biolabs (NEB), M0253L; other NEB reagents: S1330S, M0314S). ..

    Article Title: Noncanonical Wnt signaling plays an important role in modulating canonical Wnt-regulated stemness, proliferation and terminal differentiation of hepatic progenitors
    Article Snippet: Paragraph title: Total RNA isolation and touchdown-quantitative real-time PCR (TqPCR) analysis ... Total RNA was extracted according to the manufacturer's instructions and subjected to reverse transcription reactions with hexamer and M-MuLV reverse transcriptase (New England Biolabs, Ipswich, MA).

    Article Title: TET-mediated epimutagenesis of the Arabidopsis thaliana methylome
    Article Snippet: Paragraph title: DNA and RNA isolation ... One microgram of RNA was subsequently reverse transcribed with M-MuLV reverse transcriptase according to the manufacturer's instructions (NEB).

    Purification:

    Article Title: Physiological and Molecular Biological Characterization of Intracellular Carbonic Anhydrase from the Marine Diatom Phaeodactylum tricornutum 1
    Article Snippet: The cells were frozen immediately, disrupted in liquid N2 , and total RNA was extracted as described by . cDNA was synthesized with M-MuLV Reverse Transcriptase (New England Bio Labs Inc., Beverly, MA) and oligo (dT)15 primer. .. Degenerate primers were designed according to the amino acid sequence of the N-terminal end of the purified CA (PtCAF1: 5′-CGC GGA TCC GAY ATI ACI GAR ATH TTY GAY GG-3′) and according to two published nucleotide sequences of β-CAs from Coccomyxa sp. ( ) and P. purpreum ( ; PtCAR1: 5′-CCG GAA TTC CCA TNG CNK CYT GIA CHA T-3′), which corresponded to the sequences of DITEIFDG and IVQ(A/D) AW(A/D), respectively.

    Article Title: Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis
    Article Snippet: RNA was extracted and purified using a standard Trizol protocol (Thermo Fisher Scientific). .. RNA was converted to cDNA using M-MuLV Reverse Transcriptase and murine RNase inhibitor (New England BioLabs).

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: The mRNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation using NEBNext First Strand Synthesis Reaction Buffer (5x). .. Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated.

    Polymerase Chain Reaction:

    Article Title: cAMP microdomains and L-type Ca2+ channel regulation in guinea-pig ventricular myocytes
    Article Snippet: .. PCR was carried out using 0.2 μg of total RNA and M-MuLV reverse transcriptase (New England Biolabs). .. The resulting cDNA was amplified by 35 polymerase chain reaction (PCR) cycles using an annealing temperature of 60°C with primer sets specific for each EP receptor.

    Article Title: Physiological and Molecular Biological Characterization of Intracellular Carbonic Anhydrase from the Marine Diatom Phaeodactylum tricornutum 1
    Article Snippet: The cells were frozen immediately, disrupted in liquid N2 , and total RNA was extracted as described by . cDNA was synthesized with M-MuLV Reverse Transcriptase (New England Bio Labs Inc., Beverly, MA) and oligo (dT)15 primer. .. A partial nucleotide sequence of CA cDNA was amplified by PCR, on the assumption that P. tricornutum CA is homologous to β-CAs.

    Article Title: eIF4A inhibition prevents the onset of cytokine-induced muscle wasting by blocking the STAT3 and iNOS pathways
    Article Snippet: .. Reverse Transcription PCR (RT-PCR) and Quantitative PCR (qPCR) Total RNA was reverse transcribed with the M-MuLV Reverse Transcriptase (New England Biolabs). .. Resulting cDNA was diluted 1/20 and quantified using Sso Fast EvaGreen Supermix (Biorad) as described previously .

    Article Title: The Myeloid Transcription Factor KLF2 Regulates the Host Response to Polymicrobial Infection and Endotoxic Shock
    Article Snippet: Total RNA was extracted from primary macrophages, neutrophils or RAW 264.7 cells following indicated treatment using TRIzol® Reagent (Invitrogen) and 2 µg of total RNA was reverse transcribed using M-MuLV reverse transcriptase (New England Biolabs Inc.) and mixture of random and oligo-dT primers. .. Real-Time PCR was performed using Universal SYBR Green PCR Master Mix on Applied Biosystems Step One Real-Time PCR System (Applied Biosystems) using gene specific primers.

    Article Title: Noncanonical Wnt signaling plays an important role in modulating canonical Wnt-regulated stemness, proliferation and terminal differentiation of hepatic progenitors
    Article Snippet: Total RNA was extracted according to the manufacturer's instructions and subjected to reverse transcription reactions with hexamer and M-MuLV reverse transcriptase (New England Biolabs, Ipswich, MA). .. The cDNA products were further diluted and used as PCR templates.

    Article Title: Inhibition of mitochondrial respiration under hypoxia and increased antioxidant activity after reoxygenation of Tribolium castaneum
    Article Snippet: .. To prepare qRT-PCR templates, we used 2 μg of RNA to synthesize cDNA with random primers and M-Mulv reverse transcriptase (New England Biolabs, Beverly, MA, USA). qRT-PCR reactions were conducted using Power SYBR Green PCR Master Mix and run on a real-time thermal cycler (Bio-Rad, USA). ..

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated. .. The cDNA was enriched by PCR reactions using Phusion High-Fidelity DNA polymerase, universal PCR primers and index primers.

    Article Title: Two Theobroma cacao genotypes with contrasting pathogen tolerance show aberrant transcriptional and ROS responses after salicylic acid treatment
    Article Snippet: .. One microgram of RNA from each sample was reverse transcribed by M MuLV Reverse Transcriptase (New England Biolabs) using oligo-(dT)15 as a primer to generate cDNA. qRT-PCR was performed in 10 µl reactions, consisting of 5 µl of SYBR Green PCR Master Mix (Takara), 0.2 µl of Rox, and 0.4 µl of each primer, diluted to 5 µM, and 4 µl of cDNA. .. Each reaction was performed in technical duplicate using the Applied Biosystem Step One Plus Realtime PCR System (Roche) with the following programme: 15min at 94°C, 40 cycles of 15 s at 94°C, 20 s at 60°C and 40 s at 72°C.

    Article Title: Altered expression of base excision repair genes in response to high glucose-induced oxidative stress in HepG2 hepatocytes
    Article Snippet: Quantitative real-time PCR Total RNA was extracted from HepG2 cells using Trizol (Invitrogen), and cDNA was synthesized from 2 μg total RNA using M-MuLV reverse transcriptase (New England Biolabs, Beverly, MA). .. PCR primers were constructed for ogg1, DNA polymerase beta (pol β), ligase III (lig3), X-ray repair cross complementing group 1 (xrcc1) and parp1 , based on the published nucleotide sequences of human gene regions.

    Construct:

    Article Title: Altered expression of base excision repair genes in response to high glucose-induced oxidative stress in HepG2 hepatocytes
    Article Snippet: Quantitative real-time PCR Total RNA was extracted from HepG2 cells using Trizol (Invitrogen), and cDNA was synthesized from 2 μg total RNA using M-MuLV reverse transcriptase (New England Biolabs, Beverly, MA). .. PCR primers were constructed for ogg1, DNA polymerase beta (pol β), ligase III (lig3), X-ray repair cross complementing group 1 (xrcc1) and parp1 , based on the published nucleotide sequences of human gene regions.

    Mouse Assay:

    Article Title: Noncanonical Wnt signaling plays an important role in modulating canonical Wnt-regulated stemness, proliferation and terminal differentiation of hepatic progenitors
    Article Snippet: Briefly, the freshly prepared mouse liver tissues at the indicated development stages (n=5 CD1 male mice for each time point) were dissected out, quickly rinsed with PBS, minced, and then homogenized in the TRIzol reagent. .. Total RNA was extracted according to the manufacturer's instructions and subjected to reverse transcription reactions with hexamer and M-MuLV reverse transcriptase (New England Biolabs, Ipswich, MA).

    Article Title: Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis
    Article Snippet: Validation of RNA-Seq Data by Real Time PCR (qPCR) An independent group of 6-month-old male C57Bl/6 mice of the same genotype/treatment used in RNA-Seq were used for qPCR confirmation of RNA-Seq results. .. RNA was converted to cDNA using M-MuLV Reverse Transcriptase and murine RNase inhibitor (New England BioLabs).

    Plasmid Preparation:

    Article Title: C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development
    Article Snippet: C. elegans qRT-PCR Experiments Animals were synchronized by L1 arrest and grown on ampicillin and IPTG plates seeded with either E. coli HT115 (vector RNAi and mrp-5 RNAi) or E. coli OP50 (vector RNAi and mrp-5 RNAi). .. Animals were washed in M9 buffer, and total RNA was isolated using TRIzol (Invitrogen) followed by DNase I treatment and cleanup using QIAGEN RNeasy columns. cDNA was generated from 1 μg RNA using random primer and M-MuLV reverse transcriptase (New England Biolabs). qPCR was performed in technical duplicates per gene per condition using the Applied Biosystems StepOnePlus Real-Time PCR System and the Fast SYBR Green Master Mix (Thermo Fisher Scientific).

    Software:

    Article Title: Inhibition of mitochondrial respiration under hypoxia and increased antioxidant activity after reoxygenation of Tribolium castaneum
    Article Snippet: Then the raw read counts were input DEseq software to get the normalized signal for each unigene, and the fold change of unigene expression values. .. To prepare qRT-PCR templates, we used 2 μg of RNA to synthesize cDNA with random primers and M-Mulv reverse transcriptase (New England Biolabs, Beverly, MA, USA). qRT-PCR reactions were conducted using Power SYBR Green PCR Master Mix and run on a real-time thermal cycler (Bio-Rad, USA).

    Article Title: Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis
    Article Snippet: RNA was converted to cDNA using M-MuLV Reverse Transcriptase and murine RNase inhibitor (New England BioLabs). .. Primers for qPCR were predesigned KicqStart primers ordered from Sigma-Aldrich Corp. (St. Louis, MO, USA), with the exception of primers for Mt1 and Aldh1a7 , which were designed using Primer-BLAST software (National Center for Biotechnology Information, Bethesda, MD, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development
    Article Snippet: .. Animals were washed in M9 buffer, and total RNA was isolated using TRIzol (Invitrogen) followed by DNase I treatment and cleanup using QIAGEN RNeasy columns. cDNA was generated from 1 μg RNA using random primer and M-MuLV reverse transcriptase (New England Biolabs). qPCR was performed in technical duplicates per gene per condition using the Applied Biosystems StepOnePlus Real-Time PCR System and the Fast SYBR Green Master Mix (Thermo Fisher Scientific). .. Relative transcript abundance was determined using the ΔΔCt method and normalized to averaged ama-1 mRNA expression levels.

    Article Title: eIF4A inhibition prevents the onset of cytokine-induced muscle wasting by blocking the STAT3 and iNOS pathways
    Article Snippet: .. Reverse Transcription PCR (RT-PCR) and Quantitative PCR (qPCR) Total RNA was reverse transcribed with the M-MuLV Reverse Transcriptase (New England Biolabs). .. Resulting cDNA was diluted 1/20 and quantified using Sso Fast EvaGreen Supermix (Biorad) as described previously .

    Article Title: The decay of Redox-stress Response Capacity is a substantive characteristic of aging: Revising the redox theory of aging
    Article Snippet: .. RNA samples were then reverse-transcribed using M-MuLV reverse transcriptase (NEB), and mRNA levels were measured by performing RT-qPCR on a 7500 Real-Time PCR System (Applied Biosystems). ..

    Article Title: Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons
    Article Snippet: Paragraph title: Quantitative Real-Time PCR (qPCR) cDNA isolation from whole brain ... Isolated mRNA was immediately transformed into cDNA using M-MuLV Reverse Transcriptase (New England Biolabs (NEB), M0253L; other NEB reagents: S1330S, M0314S).

    Article Title: The Myeloid Transcription Factor KLF2 Regulates the Host Response to Polymicrobial Infection and Endotoxic Shock
    Article Snippet: Total RNA was extracted from primary macrophages, neutrophils or RAW 264.7 cells following indicated treatment using TRIzol® Reagent (Invitrogen) and 2 µg of total RNA was reverse transcribed using M-MuLV reverse transcriptase (New England Biolabs Inc.) and mixture of random and oligo-dT primers. .. Real-Time PCR was performed using Universal SYBR Green PCR Master Mix on Applied Biosystems Step One Real-Time PCR System (Applied Biosystems) using gene specific primers.

    Article Title: Noncanonical Wnt signaling plays an important role in modulating canonical Wnt-regulated stemness, proliferation and terminal differentiation of hepatic progenitors
    Article Snippet: Paragraph title: Total RNA isolation and touchdown-quantitative real-time PCR (TqPCR) analysis ... Total RNA was extracted according to the manufacturer's instructions and subjected to reverse transcription reactions with hexamer and M-MuLV reverse transcriptase (New England Biolabs, Ipswich, MA).

    Article Title: Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis
    Article Snippet: Paragraph title: Validation of RNA-Seq Data by Real Time PCR (qPCR) ... RNA was converted to cDNA using M-MuLV Reverse Transcriptase and murine RNase inhibitor (New England BioLabs).

    Article Title: Altered expression of base excision repair genes in response to high glucose-induced oxidative stress in HepG2 hepatocytes
    Article Snippet: .. Quantitative real-time PCR Total RNA was extracted from HepG2 cells using Trizol (Invitrogen), and cDNA was synthesized from 2 μg total RNA using M-MuLV reverse transcriptase (New England Biolabs, Beverly, MA). .. Real-time PCR was performed in a Lightcycler (Applied Biosystems, Foster City, CA) using the SYBR Green Master Mix Kit.

    Agarose Gel Electrophoresis:

    Article Title: Physiological and Molecular Biological Characterization of Intracellular Carbonic Anhydrase from the Marine Diatom Phaeodactylum tricornutum 1
    Article Snippet: The cells were frozen immediately, disrupted in liquid N2 , and total RNA was extracted as described by . cDNA was synthesized with M-MuLV Reverse Transcriptase (New England Bio Labs Inc., Beverly, MA) and oligo (dT)15 primer. .. The PCR profile was as follows: five cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 90 s, followed by 35 cycles of 94°C for 30 s, 60°C for 30 s, and 72°C for 90 s. The resulting PCR products were separated by agarose gel electrophoresis and the bands corresponding in size to approximately 400 to 600 bp were cut out of the gel and the extract was used as a template for a second PCR.

    Homogenization:

    Article Title: Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons
    Article Snippet: 2 mL TRIzol reagent and 800 μL chloroform were added after homogenization. .. Isolated mRNA was immediately transformed into cDNA using M-MuLV Reverse Transcriptase (New England Biolabs (NEB), M0253L; other NEB reagents: S1330S, M0314S).

    Random Hexamer Labeling:

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: .. Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated. .. The library fragments were purified, for a 150–200 bp size range, with AMpure XP purification kit (Beckman Coulter, MA, USA).

    Produced:

    Article Title: Two Theobroma cacao genotypes with contrasting pathogen tolerance show aberrant transcriptional and ROS responses after salicylic acid treatment
    Article Snippet: qRT-PCR measurement of selected genes qRT-PCR analyses were performed on the same RNA samples produced for the microarray experiment. .. One microgram of RNA from each sample was reverse transcribed by M MuLV Reverse Transcriptase (New England Biolabs) using oligo-(dT)15 as a primer to generate cDNA. qRT-PCR was performed in 10 µl reactions, consisting of 5 µl of SYBR Green PCR Master Mix (Takara), 0.2 µl of Rox, and 0.4 µl of each primer, diluted to 5 µM, and 4 µl of cDNA.

    Concentration Assay:

    Article Title: Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis
    Article Snippet: RNA purity and concentration was analyzed using a Nanodrop 2000c and only samples showing a 260:280 ratio of ≥1.8 and a 260:230 ratio of ≥2.0 were used (Thermo Fisher Scientific). .. RNA was converted to cDNA using M-MuLV Reverse Transcriptase and murine RNase inhibitor (New England BioLabs).

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: The RNA concentration was measured using the Qubit RNA Assay Kit on a Qubit 2.0 Fluorometer (Invitrogen Co. Ltd., San Diego, USA). .. Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated.

    CTG Assay:

    Article Title: eIF4A inhibition prevents the onset of cytokine-induced muscle wasting by blocking the STAT3 and iNOS pathways
    Article Snippet: Reverse Transcription PCR (RT-PCR) and Quantitative PCR (qPCR) Total RNA was reverse transcribed with the M-MuLV Reverse Transcriptase (New England Biolabs). .. Primers used include: STAT3 (Forward: 5′-GCTGCTTGGTGTATGGCTCT-3′, Reverse:5′-TATCTTGGCCCTTTGGAATG-3′) IL-6 (Forward:5′-AACGATGATGCACTTGCAGA-3′ Reverse:5′CTCTGAAGGACTCTGGCTTTG-3′), RPL32(Forward 5′‐TTC TTC CTC GGC GCT GCC TAC GA‐3′, Reverse 5′‐AAC CTT CTC CGC ACC CTG TTG TCA‐3′)

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New England Biolabs m mulv reverse transcriptase
    M Mulv Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 346 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mulv reverse transcriptase/product/New England Biolabs
    Average 90 stars, based on 346 article reviews
    Price from $9.99 to $1999.99
    m mulv reverse transcriptase - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results