m uridine 5  (Millipore)


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  • 91
    Name:
    Uridine 5 trihydrogen diphosphate sodium salt from Saccharomyces cerevisiae
    Description:

    Catalog Number:
    u4125
    Price:
    None
    Applications:
    UDP has been used as a purinergic agonist to study its effects on absorptive cationic flux in cochlear outer sulcus cells (OSC) and vestibular transitional cells (VTC). UDP has also been used for nucleoside diphosphatase (NDPase) activity assays in rabbit retinae.
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    Structured Review

    Millipore m uridine 5
    Uridine 5 trihydrogen diphosphate sodium salt from Saccharomyces cerevisiae

    https://www.bioz.com/result/m uridine 5/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m uridine 5 - by Bioz Stars, 2020-09
    91/100 stars

    Related Products / Commonly Used Together

    m adenosine 5 ′

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    other:

    Article Title: Biochemical Characterization of Recombinant ?-Glucosyltransferase and Analysis of Global 5-Hydroxymethylcytosine in Unique Genomes
    Article Snippet: The UDP (Sigma, catalog no. U4125) solution was made in Milli-Q water at 100 μM.

    Article Title: UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1−/− Microglia In Vitro and In Vivo
    Article Snippet: Cells were treated with 100 μ M uridine 5′-(trihydrogen diphosphate) sodium salt (UDP; U4125, Sigma Aldrich, St. Louis, MO, USA) or 50 μ M adenosine 5′-triphosphate di(tris) salt hydrate (ATP; A9062, Sigma Aldrich, St. Louis, MO, USA).

    Article Title: P2X2 Receptor Mediates Stimulation of Parasensory Cation Absorption by Cochlear Outer Sulcus Cells and Vestibular Transitional Cells
    Article Snippet: UDP (U-4125; Sigma) and ADP (A-2754; Sigma) were preincubated for 1.5 hr at room temperature with hexokinase (1 U/ml; H-4502; Sigma) and glucose (5 m m ) because the commercial preparations of UDP and ADP may be supplied with a minor component of UTP and ATP ( ).

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  • 99
    Millipore 5 fluoro 2 deoxyuridine
    5 Fluoro 2 Deoxyuridine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 fluoro 2 deoxyuridine/product/Millipore
    Average 99 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    5 fluoro 2 deoxyuridine - by Bioz Stars, 2020-09
    99/100 stars
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    91
    Millipore m udp glcnac
    A one-pot reaction for the synthesis of immunologically active O -acetylated CPSA. A , <t>UDP-3</t> O AcManNAc was chemically synthesized to test if O -acetylated CPSA could be obtained in a one-step reaction. Analysis of the reaction by high percentage PAGE followed by combined Alcian blue/silver staining did not reveal any product signals ( left lane ), whereas long CPSA was obtained in the control reaction ( right lane ). B , 31 P NMR analysis of the samples revealed that UDP-3 O AcManNAc ( top spectrum ) was not consumed by CsaB, whereas product signals were detected in the control ( bottom spectrum ). C , in vitro synthesis of O -acetylated and non- O -acetylated CPSA using all enzymes CsaA-C in a one-pot reaction. To control product formation, substrates and enzymes were added as indicated. After a 2-h incubation, products were displayed on high percentage PAGE by a combined Alcian blue/silver staining. Long chains were synthesized in all reactions containing CsaA, CsaB, and <t>UDP-GlcNAc.</t> D , only products obtained in the reaction where CsaC and acetyl-CoA were present could be detected with mAb 932 specifically directed against the CPSA OAc .
    M Udp Glcnac, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m udp glcnac/product/Millipore
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m udp glcnac - by Bioz Stars, 2020-09
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    A one-pot reaction for the synthesis of immunologically active O -acetylated CPSA. A , UDP-3 O AcManNAc was chemically synthesized to test if O -acetylated CPSA could be obtained in a one-step reaction. Analysis of the reaction by high percentage PAGE followed by combined Alcian blue/silver staining did not reveal any product signals ( left lane ), whereas long CPSA was obtained in the control reaction ( right lane ). B , 31 P NMR analysis of the samples revealed that UDP-3 O AcManNAc ( top spectrum ) was not consumed by CsaB, whereas product signals were detected in the control ( bottom spectrum ). C , in vitro synthesis of O -acetylated and non- O -acetylated CPSA using all enzymes CsaA-C in a one-pot reaction. To control product formation, substrates and enzymes were added as indicated. After a 2-h incubation, products were displayed on high percentage PAGE by a combined Alcian blue/silver staining. Long chains were synthesized in all reactions containing CsaA, CsaB, and UDP-GlcNAc. D , only products obtained in the reaction where CsaC and acetyl-CoA were present could be detected with mAb 932 specifically directed against the CPSA OAc .

    Journal: The Journal of Biological Chemistry

    Article Title: Molecular Cloning and Functional Characterization of Components of the Capsule Biosynthesis Complex of Neisseria meningitidis Serogroup A

    doi: 10.1074/jbc.M114.575142

    Figure Lengend Snippet: A one-pot reaction for the synthesis of immunologically active O -acetylated CPSA. A , UDP-3 O AcManNAc was chemically synthesized to test if O -acetylated CPSA could be obtained in a one-step reaction. Analysis of the reaction by high percentage PAGE followed by combined Alcian blue/silver staining did not reveal any product signals ( left lane ), whereas long CPSA was obtained in the control reaction ( right lane ). B , 31 P NMR analysis of the samples revealed that UDP-3 O AcManNAc ( top spectrum ) was not consumed by CsaB, whereas product signals were detected in the control ( bottom spectrum ). C , in vitro synthesis of O -acetylated and non- O -acetylated CPSA using all enzymes CsaA-C in a one-pot reaction. To control product formation, substrates and enzymes were added as indicated. After a 2-h incubation, products were displayed on high percentage PAGE by a combined Alcian blue/silver staining. Long chains were synthesized in all reactions containing CsaA, CsaB, and UDP-GlcNAc. D , only products obtained in the reaction where CsaC and acetyl-CoA were present could be detected with mAb 932 specifically directed against the CPSA OAc .

    Article Snippet: To produce sufficient CPSA (CPSAiv ) for PAGE and NMR analyses, 0.84 nmol (1.2 μ m final) of StrepII-CsaA-His6 and 37.5 pmol (50 n m final) of Δ69CsaBco -His6 in reaction buffer (50 m m Tris, pH 8.0, 20 m m MgCl2 ) were incubated with 5 m m UDP-GlcNAc and 6.8 μg of CPSAhyd of avDP6 after de- O -acetylation and dephosphorylation (CPSAhyd(deOAc) -deP).

    Techniques: Synthesized, Polyacrylamide Gel Electrophoresis, Silver Staining, Nuclear Magnetic Resonance, In Vitro, Incubation

    In vitro synthesis of CPSA. A , products synthesized in the CsaA/CsaB reaction in the presence of UDP-GlcNAc and CPSA hyd(deOAc) of avDP6 were analyzed by high percentage PAGE and a combined Alcian blue/silver staining. Long chains were produced in the presence of all reactants (reaction) and, although in small amounts, also in control 1 where no priming oligosaccharides were added. The production of long CPSA chains in control 1 argues for the capacity of CsaB to start the polymerization de novo. B , 31 P NMR analyses carried out with the reaction, and controls 1 and 4 show signals characteristic for the phosphodiester linkages of CPSA and byproducts of the reaction. C , the 1 H NMR analysis of control 4 demonstrates that CsaA catalyzes the UDP-GlcNAc/UDP-ManNAc epimerization via the intermediate 2-acetamidoglucal. D , HPLC analysis of reaction products obtained with variant CsaA:CsaB ratios, as indicated. This experiment clearly showed that UDP formation is prevented if the CsaB concentration is equal to or higher than the concentration of CsaA. UMP, UDP, and UDP-GlcNAc/UDP-ManNAc were detected at 280 nm and CPSA at 214 nm.

    Journal: The Journal of Biological Chemistry

    Article Title: Molecular Cloning and Functional Characterization of Components of the Capsule Biosynthesis Complex of Neisseria meningitidis Serogroup A

    doi: 10.1074/jbc.M114.575142

    Figure Lengend Snippet: In vitro synthesis of CPSA. A , products synthesized in the CsaA/CsaB reaction in the presence of UDP-GlcNAc and CPSA hyd(deOAc) of avDP6 were analyzed by high percentage PAGE and a combined Alcian blue/silver staining. Long chains were produced in the presence of all reactants (reaction) and, although in small amounts, also in control 1 where no priming oligosaccharides were added. The production of long CPSA chains in control 1 argues for the capacity of CsaB to start the polymerization de novo. B , 31 P NMR analyses carried out with the reaction, and controls 1 and 4 show signals characteristic for the phosphodiester linkages of CPSA and byproducts of the reaction. C , the 1 H NMR analysis of control 4 demonstrates that CsaA catalyzes the UDP-GlcNAc/UDP-ManNAc epimerization via the intermediate 2-acetamidoglucal. D , HPLC analysis of reaction products obtained with variant CsaA:CsaB ratios, as indicated. This experiment clearly showed that UDP formation is prevented if the CsaB concentration is equal to or higher than the concentration of CsaA. UMP, UDP, and UDP-GlcNAc/UDP-ManNAc were detected at 280 nm and CPSA at 214 nm.

    Article Snippet: To produce sufficient CPSA (CPSAiv ) for PAGE and NMR analyses, 0.84 nmol (1.2 μ m final) of StrepII-CsaA-His6 and 37.5 pmol (50 n m final) of Δ69CsaBco -His6 in reaction buffer (50 m m Tris, pH 8.0, 20 m m MgCl2 ) were incubated with 5 m m UDP-GlcNAc and 6.8 μg of CPSAhyd of avDP6 after de- O -acetylation and dephosphorylation (CPSAhyd(deOAc) -deP).

    Techniques: In Vitro, Synthesized, Polyacrylamide Gel Electrophoresis, Silver Staining, Produced, Nuclear Magnetic Resonance, High Performance Liquid Chromatography, Variant Assay, Concentration Assay