m tris  (Millipore)


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  • 99
    Name:
    Tris 2 carboxyethyl phosphine hydrochloride solution
    Description:
    Tris 2 carboxyethyl phosphine TCEP is very effective in cleaving disulfide bonds in aqueous solution It dissolves in water and is odorless unlike other trialkylphosphines tributylphosphine It is also less toxic than 2 mercaptoethanol These advantages make it better than the other reducing agents
    Catalog Number:
    646547
    Price:
    None
    Applications:
    Tris (2-carboxyethyl) phosphine (TCEP) can be used in several downstream applications including SDS-PAGE, mass spectrometry, labeling with cysteine specific tags, and modification of cysteine containing compounds. It prevents oxidation of protein samples, which makes it a useful buffer component as it helps to preserve enzymatic activity. It has been used in the reduction and measurement of glutathione.
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    Structured Review

    Millipore m tris
    Tris 2 carboxyethyl phosphine hydrochloride solution
    Tris 2 carboxyethyl phosphine TCEP is very effective in cleaving disulfide bonds in aqueous solution It dissolves in water and is odorless unlike other trialkylphosphines tributylphosphine It is also less toxic than 2 mercaptoethanol These advantages make it better than the other reducing agents
    https://www.bioz.com/result/m tris/product/Millipore
    Average 99 stars, based on 139 article reviews
    Price from $9.99 to $1999.99
    m tris - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Lamellipodin promotes actin assembly by clustering Ena/VASP proteins and tethering them to actin filaments
    Article Snippet: .. Actin cosedimentation assay Actin polymerization was initiated by mixing 4–20 µM A. castellani monomeric actin in buffer containing a final concentration of 20 mM HEPES [pH 7.0], 50 mM KCl, 1 mM MgCl2 , 1 mM EDTA, 1 mM ATP, 1 mM TCEP, in the absence (termed ‘native’ actin) or presence of an equal molar concentration of dark phalloidin (Calbiochem, Cat# 516640). .. In parallel, GFP-Lpd or GFP-LZ-Lpd were diluted in buffer containing a final concentration of 20 mM HEPES [pH 7.0], 50–150 mM KCl (depending on the desired final salt concentration), and 1 mM TCEP.

    Article Title: Evaluation and optimization of reduction and alkylation methods to maximize peptide identification with MS-based proteomics
    Article Snippet: .. Four reducing reagents, DTT (Sigma-Aldrich), 2-ME (Sigma-Aldrich), TCEP (Calbiochem), and THPP (Santa Cruz Biotechnology), were compared at the same concentration of 5 mM. .. Then the mixtures were cooled down to room temperature, and iodoacetamide was added to the final concentration of 14 mM to alkylate the peptides.

    Incubation:

    Article Title: Reaction Dynamics of ATP Hydrolysis Catalyzed by P-Glycoprotein
    Article Snippet: .. Solutions of 31 nM liposomes-reconstituted P-gp (200 nm E. coli lipids/cholesterol liposomes) were incubated at 37 °C with 2 mM γ-18 O4 -ATP, 50 mM Tris·HCl, 15 mM NH4 Cl, 5 mM MgSO4 , 2.5 mM EGTA, 0.02 wt % NaN3 , 2 mM TCEP, pH 7.4 in the absence (basal activity) or in presence (stimulated activity) of 50 μM nicardipine, loperamide or verapamil (from 10 mM stock solutions in dimethyl sulfoxide, Sigma-Aldrich). .. Inhibition of P-gp was studied with final concentrations of cyclosporin A of 20 μM, by dilution from a stock solution in DMSO, to yield a final DMSO concentration of 1%.

    Purification:

    Article Title: Bifunctional Chloroplastic DJ-1B from Arabidopsis thaliana is an Oxidation-Robust Holdase and a Glyoxalase Sensitive to H2O2
    Article Snippet: .. Purified AtDJ-1B was either reduced with 5 mM TCEP or oxidized by treatment with varying molar excess (2-fold, 4-fold, 6-fold, 8-fold, 10-fold, or 100-fold) of H2 O2 , or with 5 mM diamide (Sigma-Aldrich, Overijse, Belgium). .. After 1 h of incubation at 25 °C the oxidizing or reducing agents were removed by buffer exchange to Assay Buffer A using Bio-Spin® columns (Bio-Rad Laboratories N.V., Temse, Belgium).

    Activity Assay:

    Article Title: Reaction Dynamics of ATP Hydrolysis Catalyzed by P-Glycoprotein
    Article Snippet: .. Solutions of 31 nM liposomes-reconstituted P-gp (200 nm E. coli lipids/cholesterol liposomes) were incubated at 37 °C with 2 mM γ-18 O4 -ATP, 50 mM Tris·HCl, 15 mM NH4 Cl, 5 mM MgSO4 , 2.5 mM EGTA, 0.02 wt % NaN3 , 2 mM TCEP, pH 7.4 in the absence (basal activity) or in presence (stimulated activity) of 50 μM nicardipine, loperamide or verapamil (from 10 mM stock solutions in dimethyl sulfoxide, Sigma-Aldrich). .. Inhibition of P-gp was studied with final concentrations of cyclosporin A of 20 μM, by dilution from a stock solution in DMSO, to yield a final DMSO concentration of 1%.

    MTT Assay:

    Article Title: Involvement of ROS-mediated mitochondrial dysfunction and SIRT3 down-regulation in tris(2-chloroethyl)phosphate-induced cell cycle arrest
    Article Snippet: .. TCEP, dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), neutral red, 5,6-chloromethyl-2,7-dichlorodihydrofluorescein diacetate (CM-H2 DCFDA), RNAase, and propidium iodide (PI) were obtained from Sigma (Sigma-Aldrich Inc., St Louis, MO, USA). .. RPMI (Roswell Park Memorial Institute)-1640 medium and fetal bovine serum (FBS) were purchased from GIBCO® (Gibco BRL, Gaithersburg, MD, USA).

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  • 99
    Millipore m bis tris
    <t>PsbP</t> crystals from three different conditions: ( a ) 13% PEG 8000, 0.1 M <t>Tris–HCl</t> pH 7.5, 10 m M ZnCl 2 , ( b ) 18% PEG 6000, 20 m M zinc acetate, 0.1 M Tris–HCl pH 7.0 and ( c ) 16% PEG 550 MME, 0.1 M Tris–HCl pH 7.5, 10 m M ZnSO 4 .
    M Bis Tris, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m bis tris/product/Millipore
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    m bis tris - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    92
    Millipore m tris hcl
    Autocleavage of PROP. A , mass spectrometry data of <t>pro-hG1B</t> (100 μg) digested with 2 μg of trypsin or thrombin, pro-hG1B at 37 °C for 18 h in 100 μl of 10 m m <t>Tris,</t> pH 8. 5. Pro-hG1B stored at 4 °C was used as a
    M Tris Hcl, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/Millipore
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier


    Image Search Results


    PsbP crystals from three different conditions: ( a ) 13% PEG 8000, 0.1 M Tris–HCl pH 7.5, 10 m M ZnCl 2 , ( b ) 18% PEG 6000, 20 m M zinc acetate, 0.1 M Tris–HCl pH 7.0 and ( c ) 16% PEG 550 MME, 0.1 M Tris–HCl pH 7.5, 10 m M ZnSO 4 .

    Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

    Article Title: Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea

    doi: 10.1107/S1744309108040578

    Figure Lengend Snippet: PsbP crystals from three different conditions: ( a ) 13% PEG 8000, 0.1 M Tris–HCl pH 7.5, 10 m M ZnCl 2 , ( b ) 18% PEG 6000, 20 m M zinc acetate, 0.1 M Tris–HCl pH 7.0 and ( c ) 16% PEG 550 MME, 0.1 M Tris–HCl pH 7.5, 10 m M ZnSO 4 .

    Article Snippet: Fractions enriched in the His-tagged recombinant PsbP protein were pooled, washed with 20 m M bis-Tris, 0.1 M NaCl pH 6.0 (buffer E ) and finally concentrated to 15 mg ml−1 using centrifugal filter devices (Amicon Ultra 10 000 molecular-weight cutoff, 15 ml capacity) from Millipore (Billerica, Massachusetts, USA).

    Techniques:

    Difraction image of the spinach recombinant PsbP protein crystallized using 20% PEG 550 MME, 0.1 M Tris–HCl pH 7.5 and 5 m M ZnSO 4 .

    Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

    Article Title: Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea

    doi: 10.1107/S1744309108040578

    Figure Lengend Snippet: Difraction image of the spinach recombinant PsbP protein crystallized using 20% PEG 550 MME, 0.1 M Tris–HCl pH 7.5 and 5 m M ZnSO 4 .

    Article Snippet: Fractions enriched in the His-tagged recombinant PsbP protein were pooled, washed with 20 m M bis-Tris, 0.1 M NaCl pH 6.0 (buffer E ) and finally concentrated to 15 mg ml−1 using centrifugal filter devices (Amicon Ultra 10 000 molecular-weight cutoff, 15 ml capacity) from Millipore (Billerica, Massachusetts, USA).

    Techniques: Recombinant

    Autocleavage of PROP. A , mass spectrometry data of pro-hG1B (100 μg) digested with 2 μg of trypsin or thrombin, pro-hG1B at 37 °C for 18 h in 100 μl of 10 m m Tris, pH 8. 5. Pro-hG1B stored at 4 °C was used as a

    Journal: The Journal of Biological Chemistry

    Article Title: Structural Insight into the Activation Mechanism of Human Pancreatic Prophospholipase A2 *

    doi: 10.1074/jbc.M808029200

    Figure Lengend Snippet: Autocleavage of PROP. A , mass spectrometry data of pro-hG1B (100 μg) digested with 2 μg of trypsin or thrombin, pro-hG1B at 37 °C for 18 h in 100 μl of 10 m m Tris, pH 8. 5. Pro-hG1B stored at 4 °C was used as a

    Article Snippet: After purification by Superdex 75 column, pro-hG1B was buffer-exchanged to 10 m m Tris-HCl, pH 8.0, by Amicon Ultra 5k (Millipore).

    Techniques: Mass Spectrometry

    ( a ) Size-exclusion chromatography peak of Nb_PrP_01 on Superdex 75 HR 10/300; the running buffer was 20 m M Tris–HCl pH 7.5, 150 m M NaCl. ( b ) SDS–PAGE of purified Nb_PrP_01 (15% polyacrylamide gel stained with Instant Blue).

    Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

    Article Title: Crystallization and preliminary X-ray diffraction analysis of a specific VHH domain against mouse prion protein

    doi: 10.1107/S1744309110042168

    Figure Lengend Snippet: ( a ) Size-exclusion chromatography peak of Nb_PrP_01 on Superdex 75 HR 10/300; the running buffer was 20 m M Tris–HCl pH 7.5, 150 m M NaCl. ( b ) SDS–PAGE of purified Nb_PrP_01 (15% polyacrylamide gel stained with Instant Blue).

    Article Snippet: The nanobody was further purified by gel filtration on a Superdex 75 HR 10/30 column equilibrated in 20 m M Tris–HCl pH 7.5, 150 m M NaCl; fractions containing 99% pure nanobody were pooled and concentrated using an ultrafiltration unit (3000 molecular-weight cutoff, Millipore).

    Techniques: Size-exclusion Chromatography, SDS Page, Purification, Staining