m tris hcl  (Roche)


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    Structured Review

    Roche m tris hcl
    SDS–PAGE gels [12.5%( w / v ) acrylamide; coloured by the Coomassie staining technique] showing purification of the holo-His 6 <t>-HasA–HasR</t> complex. Lane 1, solubilized membrane fraction in 100 m M <t>Tris–HCl</t> pH 7.5, 2%( w / v ) ZW3-14;
    M Tris Hcl, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/Roche
    Average 95 stars, based on 687 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2020-09
    95/100 stars

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    1) Product Images from "Purification, crystallization and preliminary X-ray analysis of the outer membrane complex HasA-HasR from Serratia marcescens"

    Article Title: Purification, crystallization and preliminary X-ray analysis of the outer membrane complex HasA-HasR from Serratia marcescens

    Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

    doi: 10.1107/S1744309105041394

    SDS–PAGE gels [12.5%( w / v ) acrylamide; coloured by the Coomassie staining technique] showing purification of the holo-His 6 -HasA–HasR complex. Lane 1, solubilized membrane fraction in 100 m M Tris–HCl pH 7.5, 2%( w / v ) ZW3-14;
    Figure Legend Snippet: SDS–PAGE gels [12.5%( w / v ) acrylamide; coloured by the Coomassie staining technique] showing purification of the holo-His 6 -HasA–HasR complex. Lane 1, solubilized membrane fraction in 100 m M Tris–HCl pH 7.5, 2%( w / v ) ZW3-14;

    Techniques Used: SDS Page, Staining, Purification

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Australian multicentre comparison of subtyping methods for the investigation of Campylobacter infection
    Article Snippet: .. PCR amplifications were performed in a 50 μl volume containing 10 m m Tris–HCl (pH 8·3), 1·5 m m MgCl2 , 50 m m KCl, 200 μ m (each) dNTPs, 0·2 μ m each primer, 1·25 U of Taq DNA polymerase (Roche Diagnostics, Castle Hill, Australia) and 1 μl of Instagene™ prepared DNA. .. All PCR experiments were performed on a PC-960G gradient thermal cycler or PC-960 and FTS thermal cyclers (Corbett Research, Sydney, Australia) and the amplification products were analysed on 1% agarose gels.

    Protease Inhibitor:

    Article Title: Spontaneous Glutamatergic Synaptic Activity Regulates Constitutive COX-2 Expression in Neurons
    Article Snippet: .. Beads were washed 4× with 1 ml of buffer containing 2 m m EDTA (pH 8.0), 1% Triton X-100, 0.1% SDS, 20 m m Tris-HCL (pH 8.0), and 150 m m NaCl with 1× Complete Protease Inhibitor (Roche Applied Science) then cleared with 1 ml of final wash buffer (2 m m EDTA (pH 8.0), 1% Triton X-100, 0.1% SDS, 20 m m Tris-HCL (pH 8.0) and 500 m m NaCl with 1× Complete Protease Inhibitor (Roche Applied Science)). .. DNA-protein complexes were eluted from beads by adding 150 μl of warm (30 °C) elution buffer (1% SDS and 100 m m NaHCO3 in H2 0) followed by slow vortexing for a total of 15 min. DNA was purified with Promega Wizard SV Gel and PCR clean-up kit (product # A9281).

    Article Title: A Surface of the Kinase Domain Critical for the Allosteric Activation of G Protein-coupled Receptor Kinases *
    Article Snippet: .. After being washed with ice-cold phosphate-buffered saline, the cells were immediately lysed in 200 μl of RIPA lysis buffer containing 50 m m Tris-HCl, pH 7.5, 0.15 m NaCl, 1% Triton X-100, 1% sodium cholate, 0.1% SDS, 10 m m NaF, and protease inhibitor mixture (1836145001, Roche Applied Science) and phosphatase inhibitor mixtures (sc-45045 and sc-45065, Santa Cruz Biotechnology) at the manufacturers' recommended concentrations on ice. .. The lysate was then spun down at 16,000 × g for 15 min. 5 or 10 μl of the soluble fraction was analyzed by SDS-PAGE, transferred to polyvinylidene difluoride membrane, and then probed with mouse anti-GRK2 monoclonal antibody (C5/1) (10 μg/ml) and rabbit anti-actin polyclonal antibodies (Cytoskeleton AAN01) (1:1000 dilution).

    Article Title: Purification, crystallization and preliminary X-ray analysis of the outer membrane complex HasA-HasR from Serratia marcescens
    Article Snippet: .. The cells were harvested at 5000 rev min−1 for 10 min and resuspended in 10 m M Tris–HCl pH 7.5, 10 m M imidazole for His6 -HasA cells and 100 m M Tris–HCl pH 7.5 for HasR cells with protease-inhibitor cocktail (Roche; one tablet per 50 ml). .. The cells were disrupted in a French press by two successive cycles at 69 MPa; DNAse and RNAse were added at 1 mg ml−1 and incubated with the broken cell suspension for 15 min.

    Article Title: VEGFR-1 Regulates Adult Olfactory Bulb Neurogenesis and Migration of Neural Progenitors in the Rostral Migratory Stream In Vivo
    Article Snippet: .. Proteins were extracted in lysis buffer containing 100 m m NaCl, 20 m m Tris-HCl, pH 7.5, 1 m m EDTA, protease inhibitor (Roche), and phosphatase inhibitor solutions (Sigma). .. ELISA was performed using the Quantikine mouse sFlt-1 ELISA kit (R & D Systems) according to the protocol of the manufacturers.

    Lysis:

    Article Title: A Surface of the Kinase Domain Critical for the Allosteric Activation of G Protein-coupled Receptor Kinases *
    Article Snippet: .. After being washed with ice-cold phosphate-buffered saline, the cells were immediately lysed in 200 μl of RIPA lysis buffer containing 50 m m Tris-HCl, pH 7.5, 0.15 m NaCl, 1% Triton X-100, 1% sodium cholate, 0.1% SDS, 10 m m NaF, and protease inhibitor mixture (1836145001, Roche Applied Science) and phosphatase inhibitor mixtures (sc-45045 and sc-45065, Santa Cruz Biotechnology) at the manufacturers' recommended concentrations on ice. .. The lysate was then spun down at 16,000 × g for 15 min. 5 or 10 μl of the soluble fraction was analyzed by SDS-PAGE, transferred to polyvinylidene difluoride membrane, and then probed with mouse anti-GRK2 monoclonal antibody (C5/1) (10 μg/ml) and rabbit anti-actin polyclonal antibodies (Cytoskeleton AAN01) (1:1000 dilution).

    Article Title: VEGFR-1 Regulates Adult Olfactory Bulb Neurogenesis and Migration of Neural Progenitors in the Rostral Migratory Stream In Vivo
    Article Snippet: .. Proteins were extracted in lysis buffer containing 100 m m NaCl, 20 m m Tris-HCl, pH 7.5, 1 m m EDTA, protease inhibitor (Roche), and phosphatase inhibitor solutions (Sigma). .. ELISA was performed using the Quantikine mouse sFlt-1 ELISA kit (R & D Systems) according to the protocol of the manufacturers.

    Article Title: Activation of Phosphatidylinositol 3-Kinase Signaling Promotes Aberrant Pituitary Growth in a Mouse Model of Thyroid-Stimulating Hormone-Secreting Pituitary Tumors
    Article Snippet: .. In short, frozen pituitary was homogenized in cell lysis buffer containing 20 m m Tris-HCl (pH 7.5), 125 m m NaCl, 2 m m EDTA, 0.5% Triton X-100, 0.2 μ m okadaic acid, 2 m m Na3 VO4 , 100 m m NaF, and protease inhibitors (Complete mini-EDTA-free; Roche Diagnostics, Basel, Switzerland). ..

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    Roche m tris hcl
    Removal of Zn 2+ causes loss of structure in parkin. CD spectra of full-length parkin (3 μ m ) in 5 m m <t>Tris,</t> 20 m m <t>NaCl,</t> and 1 m m dithiothreitol at pH 7.4. The spectra show full-length parkin in the absence ( thick solid line ), and presence of
    M Tris Hcl, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/Roche
    Average 95 stars, based on 687 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

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    Removal of Zn 2+ causes loss of structure in parkin. CD spectra of full-length parkin (3 μ m ) in 5 m m Tris, 20 m m NaCl, and 1 m m dithiothreitol at pH 7.4. The spectra show full-length parkin in the absence ( thick solid line ), and presence of

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of a Novel Zn2+-binding Domain in the Autosomal Recessive Juvenile Parkinson-related E3 Ligase Parkin *-binding Domain in the Autosomal Recessive Juvenile Parkinson-related E3 Ligase Parkin * S⃞

    doi: 10.1074/jbc.M808700200

    Figure Lengend Snippet: Removal of Zn 2+ causes loss of structure in parkin. CD spectra of full-length parkin (3 μ m ) in 5 m m Tris, 20 m m NaCl, and 1 m m dithiothreitol at pH 7.4. The spectra show full-length parkin in the absence ( thick solid line ), and presence of

    Article Snippet: The E. coli cells were harvested and lysed by sonication on ice in 20 m m Tris-HCl (pH 7.4), 120 m m NaCl, 1 m m dithiothreitol buffer supplemented with EDTA-free protease inhibitor mixture (Roche Applied Science).

    Techniques:

    NMR dynamics of isolated VSV P 60 . (a) Secondary structure propensity (SSP). The SSP parameter was calculated from C α and C β secondary chemical shifts. (b) { 1 H}- 15 N heteronuclear NOEs. (c, d) Relaxation rates of longitudinal, R 1 , and transverse, R 2 , magnetization of backbone 15 N nuclei. The shaded area highlights the regions predicted to be helical from the SSP parameter (aa 2–12 and 24–39). NMR data were recorded at 600 MHz and 10°C using a peptide sample in 20 m M Tris, pH 6.0, 150 m M NaCl, 50 m M Arg, and 50 m M

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: The N0-binding region of the vesicular stomatitis virus phosphoprotein is globally disordered but contains transient ?-helices

    doi: 10.1002/pro.587

    Figure Lengend Snippet: NMR dynamics of isolated VSV P 60 . (a) Secondary structure propensity (SSP). The SSP parameter was calculated from C α and C β secondary chemical shifts. (b) { 1 H}- 15 N heteronuclear NOEs. (c, d) Relaxation rates of longitudinal, R 1 , and transverse, R 2 , magnetization of backbone 15 N nuclei. The shaded area highlights the regions predicted to be helical from the SSP parameter (aa 2–12 and 24–39). NMR data were recorded at 600 MHz and 10°C using a peptide sample in 20 m M Tris, pH 6.0, 150 m M NaCl, 50 m M Arg, and 50 m M

    Article Snippet: After an incubation of 3 h, cells were harvested by centrifugation and resuspended in 20 m M Tris-HCl, 150 m M NaCl, 50 m M Glu, and 50 m M Arg, pH 7.5 (Buffer A) supplemented with antiproteases (Complete™ Protease Inhibitor Cocktail Tablets, Roche), and cells were disrupted by sonication.

    Techniques: Nuclear Magnetic Resonance, Isolation

    SDS–PAGE gels [12.5%( w / v ) acrylamide; coloured by the Coomassie staining technique] showing purification of the holo-His 6 -HasA–HasR complex. Lane 1, solubilized membrane fraction in 100 m M Tris–HCl pH 7.5, 2%( w / v ) ZW3-14;

    Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

    Article Title: Purification, crystallization and preliminary X-ray analysis of the outer membrane complex HasA-HasR from Serratia marcescens

    doi: 10.1107/S1744309105041394

    Figure Lengend Snippet: SDS–PAGE gels [12.5%( w / v ) acrylamide; coloured by the Coomassie staining technique] showing purification of the holo-His 6 -HasA–HasR complex. Lane 1, solubilized membrane fraction in 100 m M Tris–HCl pH 7.5, 2%( w / v ) ZW3-14;

    Article Snippet: The cells were harvested at 5000 rev min−1 for 10 min and resuspended in 10 m M Tris–HCl pH 7.5, 10 m M imidazole for His6 -HasA cells and 100 m M Tris–HCl pH 7.5 for HasR cells with protease-inhibitor cocktail (Roche; one tablet per 50 ml).

    Techniques: SDS Page, Staining, Purification