Structured Review

Millipore m tris hcl
Immunochemistry of whole-cell extracts with anti-(1→5)-α-arabinan probe, LM6. A, Immunoblotting on nitrocellulose with LM6 of material separated by SDS-PAGE. Material (10 μg of protein per lane) from WT MG fruit (lanes 1–3) and Cnr MG fruit (lanes 4–6) were prepared in sample buffer immediately (lanes 1 and 4) or after incubation in <t>Tris-buffer</t> (lanes 2 and 5) or incubation in Tris-buffer plus <t>Pronase</t> E (lanes 3 and 6). Significant amounts of LM6-reactive, Pronase E-sensitive material entered the gel from Cnr , but not WT material. R indicates top of resolving gel. M shows M r markers. B, Immunodot assay on nitrocellulose of material (0.4 μg of protein per dot) analyzed in A showing abundance of LM6 epitope in all samples.
M Tris Hcl, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m tris hcl/product/Millipore
Average 99 stars, based on 1 article reviews
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m tris hcl - by Bioz Stars, 2021-09
99/100 stars

Images

1) Product Images from "Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1- > 5)-?-l-Arabinan in the Pericarp of Cnr, a Ripening Mutant of Tomato 1"

Article Title: Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1- > 5)-?-l-Arabinan in the Pericarp of Cnr, a Ripening Mutant of Tomato 1

Journal: Plant Physiology

doi:

Immunochemistry of whole-cell extracts with anti-(1→5)-α-arabinan probe, LM6. A, Immunoblotting on nitrocellulose with LM6 of material separated by SDS-PAGE. Material (10 μg of protein per lane) from WT MG fruit (lanes 1–3) and Cnr MG fruit (lanes 4–6) were prepared in sample buffer immediately (lanes 1 and 4) or after incubation in Tris-buffer (lanes 2 and 5) or incubation in Tris-buffer plus Pronase E (lanes 3 and 6). Significant amounts of LM6-reactive, Pronase E-sensitive material entered the gel from Cnr , but not WT material. R indicates top of resolving gel. M shows M r markers. B, Immunodot assay on nitrocellulose of material (0.4 μg of protein per dot) analyzed in A showing abundance of LM6 epitope in all samples.
Figure Legend Snippet: Immunochemistry of whole-cell extracts with anti-(1→5)-α-arabinan probe, LM6. A, Immunoblotting on nitrocellulose with LM6 of material separated by SDS-PAGE. Material (10 μg of protein per lane) from WT MG fruit (lanes 1–3) and Cnr MG fruit (lanes 4–6) were prepared in sample buffer immediately (lanes 1 and 4) or after incubation in Tris-buffer (lanes 2 and 5) or incubation in Tris-buffer plus Pronase E (lanes 3 and 6). Significant amounts of LM6-reactive, Pronase E-sensitive material entered the gel from Cnr , but not WT material. R indicates top of resolving gel. M shows M r markers. B, Immunodot assay on nitrocellulose of material (0.4 μg of protein per dot) analyzed in A showing abundance of LM6 epitope in all samples.

Techniques Used: SDS Page, Incubation

2) Product Images from "Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1- > 5)-?-l-Arabinan in the Pericarp of Cnr, a Ripening Mutant of Tomato 1"

Article Title: Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1- > 5)-?-l-Arabinan in the Pericarp of Cnr, a Ripening Mutant of Tomato 1

Journal: Plant Physiology

doi:

Immunochemistry of whole-cell extracts with anti-(1→5)-α-arabinan probe, LM6. A, Immunoblotting on nitrocellulose with LM6 of material separated by SDS-PAGE. Material (10 μg of protein per lane) from WT MG fruit (lanes 1–3) and Cnr MG fruit (lanes 4–6) were prepared in sample buffer immediately (lanes 1 and 4) or after incubation in Tris-buffer (lanes 2 and 5) or incubation in Tris-buffer plus Pronase E (lanes 3 and 6). Significant amounts of LM6-reactive, Pronase E-sensitive material entered the gel from Cnr , but not WT material. R indicates top of resolving gel. M shows M r markers. B, Immunodot assay on nitrocellulose of material (0.4 μg of protein per dot) analyzed in A showing abundance of LM6 epitope in all samples.
Figure Legend Snippet: Immunochemistry of whole-cell extracts with anti-(1→5)-α-arabinan probe, LM6. A, Immunoblotting on nitrocellulose with LM6 of material separated by SDS-PAGE. Material (10 μg of protein per lane) from WT MG fruit (lanes 1–3) and Cnr MG fruit (lanes 4–6) were prepared in sample buffer immediately (lanes 1 and 4) or after incubation in Tris-buffer (lanes 2 and 5) or incubation in Tris-buffer plus Pronase E (lanes 3 and 6). Significant amounts of LM6-reactive, Pronase E-sensitive material entered the gel from Cnr , but not WT material. R indicates top of resolving gel. M shows M r markers. B, Immunodot assay on nitrocellulose of material (0.4 μg of protein per dot) analyzed in A showing abundance of LM6 epitope in all samples.

Techniques Used: SDS Page, Incubation

3) Product Images from "Unraveling the Redox Properties of the Global Regulator FurA from Anabaena sp. PCC 7120: Disulfide Reductase Activity Based on Its CXXC Motifs"

Article Title: Unraveling the Redox Properties of the Global Regulator FurA from Anabaena sp. PCC 7120: Disulfide Reductase Activity Based on Its CXXC Motifs

Journal: Antioxidants & Redox Signaling

doi: 10.1089/ars.2013.5376

Disulfide reductase activity of FurA mutant proteins. (A) Insulin reduction assays were performed in the reaction buffer containing 0.13 m M insulin, 50 m M Tris/HCl 7.5, 100 m M NaCl, 2 m M EDTA, and 10 μ M
Figure Legend Snippet: Disulfide reductase activity of FurA mutant proteins. (A) Insulin reduction assays were performed in the reaction buffer containing 0.13 m M insulin, 50 m M Tris/HCl 7.5, 100 m M NaCl, 2 m M EDTA, and 10 μ M

Techniques Used: Activity Assay, Mutagenesis

4) Product Images from "Structural and biochemical characterization of a metagenome-derived esterase with a long N-terminal extension"

Article Title: Structural and biochemical characterization of a metagenome-derived esterase with a long N-terminal extension

Journal: Protein Science : A Publication of the Protein Society

doi: 10.1002/pro.2591

Thermal denaturation LC-Est1 and LC-Est1C. The thermal denaturation curves of LC-Est1 (solid line) and LC-Est1C (broken line) measured in 10 m M Tris-HCl (pH 7.0) are shown. The curves were recorded by monitoring the change in CD values at 222 nm as described
Figure Legend Snippet: Thermal denaturation LC-Est1 and LC-Est1C. The thermal denaturation curves of LC-Est1 (solid line) and LC-Est1C (broken line) measured in 10 m M Tris-HCl (pH 7.0) are shown. The curves were recorded by monitoring the change in CD values at 222 nm as described

Techniques Used:

Far-UV CD spectra. The far-UV CD spectra of LC-Est1 (solid line) and LC-Est1C (broken line) were measured at 25°C in 10 m M Tris-HCl (pH 7.0) as described in Materials and Methods.
Figure Legend Snippet: Far-UV CD spectra. The far-UV CD spectra of LC-Est1 (solid line) and LC-Est1C (broken line) were measured at 25°C in 10 m M Tris-HCl (pH 7.0) as described in Materials and Methods.

Techniques Used:

5) Product Images from "Structural Insight into the Activation Mechanism of Human Pancreatic Prophospholipase A2 *"

Article Title: Structural Insight into the Activation Mechanism of Human Pancreatic Prophospholipase A2 *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M808029200

Autocleavage of PROP. A , mass spectrometry data of pro-hG1B (100 μg) digested with 2 μg of trypsin or thrombin, pro-hG1B at 37 °C for 18 h in 100 μl of 10 m m Tris, pH 8. 5. Pro-hG1B stored at 4 °C was used as a
Figure Legend Snippet: Autocleavage of PROP. A , mass spectrometry data of pro-hG1B (100 μg) digested with 2 μg of trypsin or thrombin, pro-hG1B at 37 °C for 18 h in 100 μl of 10 m m Tris, pH 8. 5. Pro-hG1B stored at 4 °C was used as a

Techniques Used: Mass Spectrometry

6) Product Images from "Identification of the Major Cysteine Protease of Giardia and Its Role in Encystation * and Its Role in Encystation * S⃞"

Article Title: Identification of the Major Cysteine Protease of Giardia and Its Role in Encystation * and Its Role in Encystation * S⃞

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M802133200

r Gl CP2 processes recombinant cyst wall protein 2 (rCWP2) to the predicted 26-kDa size necessary for incorporation into the cyst wall. A , in-solution cleavage of 35 S-labeled rCWP2 by purified r Gl CP2. rCWP2 was exposed to r Gl CP2 in Tris buffer, pH 7.6, and incubated at 25 °C for 0, 5, 15, 45, 90, and 120 min r Gl CP2 can cleave the 39-kDa rCWP2 to a 26-kDa fragment ( arrowheads ) in a time-dependent manner. B , rCWP2 was incubated for 35 min with various proteases. Peak A from anion exchange purification of r Gl CP2 ( lane 1 ) and R. norvegicus cathepsin C ( lane 6 ) did not process rCWP2 from its full-length size of 39 kDa ( arrowhead ). r Gl CP2 ( lane 2 ) and Giardia lysates ( lane 3 ) exhibited identical processing patterns of rCWP2. Other endopeptidases, such as trypsin ( lane 4 ) and chymotrypsin ( lane 5 ), can also process rCWP2 to 26 kDa ( arrowhead ). Lanes 5 and 6 are set apart as these samples were run on separate gels. C , an endopeptidase inhibitor, K11777 ( panel C ) does not ( panel B ). Panel A is an uninhibited control.
Figure Legend Snippet: r Gl CP2 processes recombinant cyst wall protein 2 (rCWP2) to the predicted 26-kDa size necessary for incorporation into the cyst wall. A , in-solution cleavage of 35 S-labeled rCWP2 by purified r Gl CP2. rCWP2 was exposed to r Gl CP2 in Tris buffer, pH 7.6, and incubated at 25 °C for 0, 5, 15, 45, 90, and 120 min r Gl CP2 can cleave the 39-kDa rCWP2 to a 26-kDa fragment ( arrowheads ) in a time-dependent manner. B , rCWP2 was incubated for 35 min with various proteases. Peak A from anion exchange purification of r Gl CP2 ( lane 1 ) and R. norvegicus cathepsin C ( lane 6 ) did not process rCWP2 from its full-length size of 39 kDa ( arrowhead ). r Gl CP2 ( lane 2 ) and Giardia lysates ( lane 3 ) exhibited identical processing patterns of rCWP2. Other endopeptidases, such as trypsin ( lane 4 ) and chymotrypsin ( lane 5 ), can also process rCWP2 to 26 kDa ( arrowhead ). Lanes 5 and 6 are set apart as these samples were run on separate gels. C , an endopeptidase inhibitor, K11777 ( panel C ) does not ( panel B ). Panel A is an uninhibited control.

Techniques Used: Recombinant, Labeling, Purification, Incubation

7) Product Images from "Crystallization and preliminary X-ray diffraction analysis of a specific VHH domain against mouse prion protein"

Article Title: Crystallization and preliminary X-ray diffraction analysis of a specific VHH domain against mouse prion protein

Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

doi: 10.1107/S1744309110042168

( a ) Size-exclusion chromatography peak of Nb_PrP_01 on Superdex 75 HR 10/300; the running buffer was 20 m M Tris–HCl pH 7.5, 150 m M NaCl. ( b ) SDS–PAGE of purified Nb_PrP_01 (15% polyacrylamide gel stained with Instant Blue).
Figure Legend Snippet: ( a ) Size-exclusion chromatography peak of Nb_PrP_01 on Superdex 75 HR 10/300; the running buffer was 20 m M Tris–HCl pH 7.5, 150 m M NaCl. ( b ) SDS–PAGE of purified Nb_PrP_01 (15% polyacrylamide gel stained with Instant Blue).

Techniques Used: Size-exclusion Chromatography, SDS Page, Purification, Staining

Related Articles

Purification:

Article Title: A Cu(II)-MOF Based on a Propargyl Carbamate-Functionalized Isophthalate Ligand as Nitrite Electrochemical Sensor
Article Snippet: .. All reagents and solvents were purchased from commercial vendors and used as received, tris(hydroxymethyl)aminomethane hydrochloride buffer (TRIS) was purchased from Sigma Aldrich; ultrapure water purified with the Milli-Q plus system (Millipore Co, resistivity over 18 MΩ cm) was used in all cases. ..

other:

Article Title: Unilateral Ureteral Obstruction for 28 Days in Rats Is Not Associated with Changes in Cardiac Function or Alterations in Mitochondrial Function
Article Snippet: The reagents used in this study were adenosine diphosphate (ADP) sodium salt, antimycin A, bovine serum albumin (BSA), fatty acid (FA)-free BSA, carbonyl cyanide m-chlorophenylhydrazone (CCCP), D-mannitol, eosin, ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), ethylene glycol-bis(2-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), hematoxylin, horseradish peroxidase (HRP), K-lactobionate, magnesium chloride (MgCl2 ), nicotinamide adenine dinucleotide 2′-phosphate-reduced (NADPH), nicotinamide adenine dinucleotide phosphate (NADP+ ), oligomycin, phenylmethylsulfonyl fluoride (PMSF), rotenone, safranin O, Sirius red, sodium chloride (NaCl), sodium dodecyl sulfate (SDS), sodium glutamate, sodium malate, sodium phosphate monobasic (NaH2 PO4 ), sodium pyruvate, sodium succinate dibasic, sucrose, taurine, and tris hydrochloride (Tris-HCl), which were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Article Title: Evaluation of 1β-Hydroxylation of Deoxycholic Acid as a Non-Invasive Urinary Biomarker of CYP3A Activity in the Assessment of Inhibition-Based Drug–Drug Interaction in Healthy Volunteers
Article Snippet: MDZ, 1′-OH-MDZ, DCA, diazepam, activated charcoal (DARCO® G-60, 100 mesh powder), and TRIS hydrochloride were obtained from Sigma Aldrich (GmbH, Germany).

Article Title: Hericium erinaceus (Bull.) Pers. Ethanolic Extract with Antioxidant Properties on Scopolamine-Induced Memory Deficits in a Zebrafish Model of Cognitive Impairment
Article Snippet: The saline phosphate buffer, acetic acid, Tris hydrochloride, were all purchased from Sigma-Aldrich (Steinheim, Germany), and ethyl alcohol (96%) from Prodvinalco S.A. (Cluj-Napoca, Romania).

Lysis:

Article Title: Poly (ADP-Ribose) Polymerase Inhibitor, ABT888, Improved Cisplatin Effect in Human Oral Cell Carcinoma
Article Snippet: .. The lysis buffer contained 0.1 mM phenylmethyl sulfonyl fluoride, 2 mM EDTA, 25 mM-glycerophosphate, 0.1 mM sodium orthovanadate, 25 mM sodium fluoride, 5 µg of leupeptin, 0.2% Triton X-100 (Sigma-Aldrich Chemical Co, St Louis, MO, USA) and 0.3% Nonidet p-40 (Sigma-Aldrich Chemical Co) in 50 mM Tris-hydrochloride (Sigma-Aldrich Chemical Co)/150mM sodium chloride (pH 7.5). ..

Article Title: The effect of parathyroid hormone on osteogenesis is mediated partly by osteolectin
Article Snippet: .. Prior to extracting proteins, cells were washed with PBS and then lysis buffer was added (50 mM Tris⋅HCl, 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM sodium vanadate, 0.5 mM sodium fluoride, and cOmplete Mini EDTA-free Protease Inhibitor Mixture [Sigma]). ..

Protease Inhibitor:

Article Title: The effect of parathyroid hormone on osteogenesis is mediated partly by osteolectin
Article Snippet: .. Prior to extracting proteins, cells were washed with PBS and then lysis buffer was added (50 mM Tris⋅HCl, 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM sodium vanadate, 0.5 mM sodium fluoride, and cOmplete Mini EDTA-free Protease Inhibitor Mixture [Sigma]). ..

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    Millipore m tris hcl
    Immunochemistry of whole-cell extracts with anti-(1→5)-α-arabinan probe, LM6. A, Immunoblotting on nitrocellulose with LM6 of material separated by SDS-PAGE. Material (10 μg of protein per lane) from WT MG fruit (lanes 1–3) and Cnr MG fruit (lanes 4–6) were prepared in sample buffer immediately (lanes 1 and 4) or after incubation in <t>Tris-buffer</t> (lanes 2 and 5) or incubation in Tris-buffer plus <t>Pronase</t> E (lanes 3 and 6). Significant amounts of LM6-reactive, Pronase E-sensitive material entered the gel from Cnr , but not WT material. R indicates top of resolving gel. M shows M r markers. B, Immunodot assay on nitrocellulose of material (0.4 μg of protein per dot) analyzed in A showing abundance of LM6 epitope in all samples.
    M Tris Hcl, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    93
    Millipore m tris hcl buffer
    A crystal of Sa <t>TenA</t> (0.1 × 0.1 × 0.4 mm; the scale bar is 0.1 mm in length) obtained using 0.2 M sodium acetate, 0.1 M <t>Tris–HCl</t> pH 8.5 and 28%( w / v ) PEG 3350.
    M Tris Hcl Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl buffer/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m tris hcl buffer - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Immunochemistry of whole-cell extracts with anti-(1→5)-α-arabinan probe, LM6. A, Immunoblotting on nitrocellulose with LM6 of material separated by SDS-PAGE. Material (10 μg of protein per lane) from WT MG fruit (lanes 1–3) and Cnr MG fruit (lanes 4–6) were prepared in sample buffer immediately (lanes 1 and 4) or after incubation in Tris-buffer (lanes 2 and 5) or incubation in Tris-buffer plus Pronase E (lanes 3 and 6). Significant amounts of LM6-reactive, Pronase E-sensitive material entered the gel from Cnr , but not WT material. R indicates top of resolving gel. M shows M r markers. B, Immunodot assay on nitrocellulose of material (0.4 μg of protein per dot) analyzed in A showing abundance of LM6 epitope in all samples.

    Journal: Plant Physiology

    Article Title: Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1- > 5)-?-l-Arabinan in the Pericarp of Cnr, a Ripening Mutant of Tomato 1

    doi:

    Figure Lengend Snippet: Immunochemistry of whole-cell extracts with anti-(1→5)-α-arabinan probe, LM6. A, Immunoblotting on nitrocellulose with LM6 of material separated by SDS-PAGE. Material (10 μg of protein per lane) from WT MG fruit (lanes 1–3) and Cnr MG fruit (lanes 4–6) were prepared in sample buffer immediately (lanes 1 and 4) or after incubation in Tris-buffer (lanes 2 and 5) or incubation in Tris-buffer plus Pronase E (lanes 3 and 6). Significant amounts of LM6-reactive, Pronase E-sensitive material entered the gel from Cnr , but not WT material. R indicates top of resolving gel. M shows M r markers. B, Immunodot assay on nitrocellulose of material (0.4 μg of protein per dot) analyzed in A showing abundance of LM6 epitope in all samples.

    Article Snippet: The powder (2 mL) was suspended in 50 m m Tris-HCl, pH 6.5, or 50 m m Tris-HCl, pH 6.5, containing Pronase E (Sigma) at 1 mg mL−1 .

    Techniques: SDS Page, Incubation

    Disulfide reductase activity of FurA mutant proteins. (A) Insulin reduction assays were performed in the reaction buffer containing 0.13 m M insulin, 50 m M Tris/HCl 7.5, 100 m M NaCl, 2 m M EDTA, and 10 μ M

    Journal: Antioxidants & Redox Signaling

    Article Title: Unraveling the Redox Properties of the Global Regulator FurA from Anabaena sp. PCC 7120: Disulfide Reductase Activity Based on Its CXXC Motifs

    doi: 10.1089/ars.2013.5376

    Figure Lengend Snippet: Disulfide reductase activity of FurA mutant proteins. (A) Insulin reduction assays were performed in the reaction buffer containing 0.13 m M insulin, 50 m M Tris/HCl 7.5, 100 m M NaCl, 2 m M EDTA, and 10 μ M

    Article Snippet: After washing with buffer A, FurA-GST protein was eluted with 50 m M Tris/HCl pH 8 containing 30 m M reduced GSH (Sigma) and concentrated by centrifugation in 10 kDa Centricon devices (Millipore).

    Techniques: Activity Assay, Mutagenesis

    A crystal of Sa TenA (0.1 × 0.1 × 0.4 mm; the scale bar is 0.1 mm in length) obtained using 0.2 M sodium acetate, 0.1 M Tris–HCl pH 8.5 and 28%( w / v ) PEG 3350.

    Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

    Article Title: Purification, crystallization and preliminary X-ray diffraction analysis of the thiaminase type II from Staphylococcus aureus

    doi: 10.1107/S1744309110043174

    Figure Lengend Snippet: A crystal of Sa TenA (0.1 × 0.1 × 0.4 mm; the scale bar is 0.1 mm in length) obtained using 0.2 M sodium acetate, 0.1 M Tris–HCl pH 8.5 and 28%( w / v ) PEG 3350.

    Article Snippet: The purified Sa TenA was dialyzed against 100 m M Tris–HCl buffer pH 8.0; it was then concentrated to 10 mg ml−1 using an Amicon Ultra centrifugal filter device (Millipore; 3 kDa molecular-weight cutoff) and used for initial screening of crystallization conditions.

    Techniques: