m tris base  (Bio-Rad)

 
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  • 99
    Name:
    Tris
    Description:
    500 g 99 8 pure Tris base Tris hydroxymethyl aminomethane or Trimethamine
    Catalog Number:
    1610716
    Price:
    None
    Category:
    Mini Sub Cell GT Cell
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    Structured Review

    Bio-Rad m tris base
    Tris
    500 g 99 8 pure Tris base Tris hydroxymethyl aminomethane or Trimethamine
    https://www.bioz.com/result/m tris base/product/Bio-Rad
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    m tris base - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Francisella tularensis 2-C-Methyl-D-Erythritol 4-Phosphate Cytidylyltransferase: Kinetic Characterization and Phosphoregulation
    Article Snippet: .. Size-Exclusion Chromatography The molecular mass of MEP cytidylyltransferase and the mutant derivatives were estimated by loading 1 mg of protein onto a Sephacryl 200HR (Sigma Aldrich, St. Louis, MO) size-exclusion chromatography column equilibrated with 0.1 M Tris pH 7.5, 1 mM NaCl (flow rate of 2 mL/min) and calibrated with a gel filtration standard kit purchased from Bio-Rad (Hercules, CA). ..

    Filtration:

    Article Title: Francisella tularensis 2-C-Methyl-D-Erythritol 4-Phosphate Cytidylyltransferase: Kinetic Characterization and Phosphoregulation
    Article Snippet: .. Size-Exclusion Chromatography The molecular mass of MEP cytidylyltransferase and the mutant derivatives were estimated by loading 1 mg of protein onto a Sephacryl 200HR (Sigma Aldrich, St. Louis, MO) size-exclusion chromatography column equilibrated with 0.1 M Tris pH 7.5, 1 mM NaCl (flow rate of 2 mL/min) and calibrated with a gel filtration standard kit purchased from Bio-Rad (Hercules, CA). ..

    Mutagenesis:

    Article Title: Francisella tularensis 2-C-Methyl-D-Erythritol 4-Phosphate Cytidylyltransferase: Kinetic Characterization and Phosphoregulation
    Article Snippet: .. Size-Exclusion Chromatography The molecular mass of MEP cytidylyltransferase and the mutant derivatives were estimated by loading 1 mg of protein onto a Sephacryl 200HR (Sigma Aldrich, St. Louis, MO) size-exclusion chromatography column equilibrated with 0.1 M Tris pH 7.5, 1 mM NaCl (flow rate of 2 mL/min) and calibrated with a gel filtration standard kit purchased from Bio-Rad (Hercules, CA). ..

    Size-exclusion Chromatography:

    Article Title: Francisella tularensis 2-C-Methyl-D-Erythritol 4-Phosphate Cytidylyltransferase: Kinetic Characterization and Phosphoregulation
    Article Snippet: .. Size-Exclusion Chromatography The molecular mass of MEP cytidylyltransferase and the mutant derivatives were estimated by loading 1 mg of protein onto a Sephacryl 200HR (Sigma Aldrich, St. Louis, MO) size-exclusion chromatography column equilibrated with 0.1 M Tris pH 7.5, 1 mM NaCl (flow rate of 2 mL/min) and calibrated with a gel filtration standard kit purchased from Bio-Rad (Hercules, CA). ..

    Blocking Assay:

    Article Title: Immunity to LuloHya and Lundep, the salivary spreading factors from Lutzomyia longipalpis, protects against Leishmania major infection
    Article Snippet: .. Proteins were transferred to a nitrocellulose membrane (iBlot, Invitrogene) that was blocked overnight at 4°C with blocking buffer: 50 mM Tris, 150 mM NaCl containing 5% (w/v) powdered nonfat blotting-grade milk (Bio-Rad) and 0.05% of Tween-20 (Sigma). .. Mouse anti-LuloHya antibodies were diluted in blocking buffer (1:5,000) and incubated for 90 min.

    other:

    Article Title: Proteome Analysis Using Gel-LC-MS/MS
    Article Snippet: SDS Resuspension Buffer: 1% (w/v) SDS in 100 mM Tris-HCL, pH 8.0 Dissolve 0.606 g Tris (Bio-Rad) in 40 ml water.

    Stripping Membranes:

    Article Title: Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic Approach
    Article Snippet: .. After the alkylation step, each strip was quickly washed with MOPS running buffer pH 7,7 (50 mM MOPS, 50 mM Tris, 0,1% (w/v), and 1 mM EDTA) and loaded on 13,3 × 8,7 cm Criterion XT gels (Bio-Rad) with the wells previously filled with an agarose solution for 2DE, composed of 0,5% (w/v) low melting point agarose, 25 mM Tris, 192 mM glycine, 0,1% (w/v) SDS, and vestigial quantities of bromophenol blue (Bio-Rad). .. Additionally, 8 μ L of SeeBlue Pre-Stained Standard (Invitrogen) were added to the gel where the strip containing 250 μ g of protein was loaded.

    Two-Dimensional Gel Electrophoresis:

    Article Title: Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic Approach
    Article Snippet: .. After the alkylation step, each strip was quickly washed with MOPS running buffer pH 7,7 (50 mM MOPS, 50 mM Tris, 0,1% (w/v), and 1 mM EDTA) and loaded on 13,3 × 8,7 cm Criterion XT gels (Bio-Rad) with the wells previously filled with an agarose solution for 2DE, composed of 0,5% (w/v) low melting point agarose, 25 mM Tris, 192 mM glycine, 0,1% (w/v) SDS, and vestigial quantities of bromophenol blue (Bio-Rad). .. Additionally, 8 μ L of SeeBlue Pre-Stained Standard (Invitrogen) were added to the gel where the strip containing 250 μ g of protein was loaded.

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  • 99
    Bio-Rad m tris base
    M Tris Base, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris base/product/Bio-Rad
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    m tris base - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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