m smegmatis atcc 700084  (ATCC)


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    ATCC m smegmatis atcc 700084
    M Smegmatis Atcc 700084, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m smegmatis atcc 19420  (ATCC)


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    ATCC m smegmatis atcc 19420
    Live ELISA showing binding activity of MABs GG9 (graphs A and C) and JG7 (graphs B and D) at concentrations 5, 10, and 25 µg/mL to M. tuberculosis (H37Ra; ATCC® 25177™), M. smegmatis (ATCC® 19420™; as a non-pathogenic control), M. avium subsp. avium (ATCC® 15769™), M. bovis (BCG; ATCC® 27290™), M. fortuitum subsp. fortuitum (ATCC® 6841™), and M. intracellulare (ATCC® 13950™) at bacterial dilutions of 4 × 10 5 CFU/mL (neat/undiluted) and 2 × 10 5 CFU/mL (1:2). ELISA, enzyme-linked immunosorbent assay; MABs, monoclonal antibodies; CFU/mL, colony-forming unit per millimeter.
    M Smegmatis Atcc 19420, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "In vitro evaluation of the binding activity of novel mouse IgG1 opsonic monoclonal antibodies to Mycobacterium tuberculosis and other selected mycobacterial species"

    Article Title: In vitro evaluation of the binding activity of novel mouse IgG1 opsonic monoclonal antibodies to Mycobacterium tuberculosis and other selected mycobacterial species

    Journal: Journal of Clinical Tuberculosis and Other Mycobacterial Diseases

    doi: 10.1016/j.jctube.2024.100435

    Live ELISA showing binding activity of MABs GG9 (graphs A and C) and JG7 (graphs B and D) at concentrations 5, 10, and 25 µg/mL to M. tuberculosis (H37Ra; ATCC® 25177™), M. smegmatis (ATCC® 19420™; as a non-pathogenic control), M. avium subsp. avium (ATCC® 15769™), M. bovis (BCG; ATCC® 27290™), M. fortuitum subsp. fortuitum (ATCC® 6841™), and M. intracellulare (ATCC® 13950™) at bacterial dilutions of 4 × 10 5 CFU/mL (neat/undiluted) and 2 × 10 5 CFU/mL (1:2). ELISA, enzyme-linked immunosorbent assay; MABs, monoclonal antibodies; CFU/mL, colony-forming unit per millimeter.
    Figure Legend Snippet: Live ELISA showing binding activity of MABs GG9 (graphs A and C) and JG7 (graphs B and D) at concentrations 5, 10, and 25 µg/mL to M. tuberculosis (H37Ra; ATCC® 25177™), M. smegmatis (ATCC® 19420™; as a non-pathogenic control), M. avium subsp. avium (ATCC® 15769™), M. bovis (BCG; ATCC® 27290™), M. fortuitum subsp. fortuitum (ATCC® 6841™), and M. intracellulare (ATCC® 13950™) at bacterial dilutions of 4 × 10 5 CFU/mL (neat/undiluted) and 2 × 10 5 CFU/mL (1:2). ELISA, enzyme-linked immunosorbent assay; MABs, monoclonal antibodies; CFU/mL, colony-forming unit per millimeter.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Binding Assay, Activity Assay

    m smegmatis atcc 10231  (ATCC)


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    ATCC m smegmatis atcc 10231
    M Smegmatis Atcc 10231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m smegmatis atcc 10231  (ATCC)


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    ATCC m smegmatis atcc 10231
    M Smegmatis Atcc 10231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m smegmatis atcc 607  (ATCC)


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    ATCC m smegmatis atcc 607
    M Smegmatis Atcc 607, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m smegmatis atcc 19420  (ATCC)


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    ATCC m smegmatis atcc 19420
    M Smegmatis Atcc 19420, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m smegmatis atcc 700084  (ATCC)


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    ATCC m smegmatis atcc 700084
    M Smegmatis Atcc 700084, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m smegmatis atcc 700084  (ATCC)


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    ATCC m smegmatis atcc 700084
    M Smegmatis Atcc 700084, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m smegmatis atcc  (ATCC)


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    ATCC m smegmatis atcc
    (A) Mycobacterial cell-wall compositions. (B) 13 C CPMAS spectra of mAGP from M. <t>smegmatis</t> at OD 1.7, OD 3.0, and OD 5.2. Inset shows the growth curve of M. smegmatis in Middlebrook 7H9 Broth with 0.2% glycerol, 10% ADC enrichment and 0.05% Tween-80. (C) 13 C CPMAS spectra comparison of M. smegmatis mAGP from cells harvested at OD 3.0, and the isolated AGP after removal of MA. (D) 13 C CPMAS spectral comparison of M. smegmatis AGP and PG isolated from cells harvested at OD 3.0. Each spectrum is the result of 40,960 scans with sample masses of 22.3 mg mAGP and 7.7 mg PG. (E) 13 C CPMAS spectral comparison of M. smegmatis mAGP harvested at OD 1.0 with spectra acquired as a function of CP time (10.7 mg, 8192 scans each spectrum). (F) Quantitative CP array plot of selected peak intensities of M. smegmatis mAGP spectra obtained as a function of CP time.
    M Smegmatis Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells"

    Article Title: CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells

    Journal: Journal of magnetic resonance open

    doi: 10.1016/j.jmro.2023.100127

    (A) Mycobacterial cell-wall compositions. (B) 13 C CPMAS spectra of mAGP from M. smegmatis at OD 1.7, OD 3.0, and OD 5.2. Inset shows the growth curve of M. smegmatis in Middlebrook 7H9 Broth with 0.2% glycerol, 10% ADC enrichment and 0.05% Tween-80. (C) 13 C CPMAS spectra comparison of M. smegmatis mAGP from cells harvested at OD 3.0, and the isolated AGP after removal of MA. (D) 13 C CPMAS spectral comparison of M. smegmatis AGP and PG isolated from cells harvested at OD 3.0. Each spectrum is the result of 40,960 scans with sample masses of 22.3 mg mAGP and 7.7 mg PG. (E) 13 C CPMAS spectral comparison of M. smegmatis mAGP harvested at OD 1.0 with spectra acquired as a function of CP time (10.7 mg, 8192 scans each spectrum). (F) Quantitative CP array plot of selected peak intensities of M. smegmatis mAGP spectra obtained as a function of CP time.
    Figure Legend Snippet: (A) Mycobacterial cell-wall compositions. (B) 13 C CPMAS spectra of mAGP from M. smegmatis at OD 1.7, OD 3.0, and OD 5.2. Inset shows the growth curve of M. smegmatis in Middlebrook 7H9 Broth with 0.2% glycerol, 10% ADC enrichment and 0.05% Tween-80. (C) 13 C CPMAS spectra comparison of M. smegmatis mAGP from cells harvested at OD 3.0, and the isolated AGP after removal of MA. (D) 13 C CPMAS spectral comparison of M. smegmatis AGP and PG isolated from cells harvested at OD 3.0. Each spectrum is the result of 40,960 scans with sample masses of 22.3 mg mAGP and 7.7 mg PG. (E) 13 C CPMAS spectral comparison of M. smegmatis mAGP harvested at OD 1.0 with spectra acquired as a function of CP time (10.7 mg, 8192 scans each spectrum). (F) Quantitative CP array plot of selected peak intensities of M. smegmatis mAGP spectra obtained as a function of CP time.

    Techniques Used: Comparison, Isolation

    (A) 13 C CPMAS spectra of pure MA from M. tuberculosis (15.3 mg), AG from Larchwood (28.2 mg), and PG isolated from M. smegmatis (7.7 mg). (B) 13 C CPMAS spectrum of M. smegmatis mAGP from cells harvested at OD 1.0, and a reconstituted spectrum generated by adding 50% PG, 20% AG, and 30% AG from spectra in . Each spectrum is the result of 40,960 scans.
    Figure Legend Snippet: (A) 13 C CPMAS spectra of pure MA from M. tuberculosis (15.3 mg), AG from Larchwood (28.2 mg), and PG isolated from M. smegmatis (7.7 mg). (B) 13 C CPMAS spectrum of M. smegmatis mAGP from cells harvested at OD 1.0, and a reconstituted spectrum generated by adding 50% PG, 20% AG, and 30% AG from spectra in . Each spectrum is the result of 40,960 scans.

    Techniques Used: Isolation, Generated

    (A) 13 C CPMAS spectra of mAGP from M. smegmatis at OD 1.0 (24.5 mg), and mAGP from M. abscessus at OD 0.92 (10.3 mg). (B) 13 C CPMAS spectral overlay of the M. abscessus mAGP and a reconstituted spectrum generated by adding 35% PG, 35% AG, and 30% MA from spectra in . Each spectrum is the result of 40,960 scans.
    Figure Legend Snippet: (A) 13 C CPMAS spectra of mAGP from M. smegmatis at OD 1.0 (24.5 mg), and mAGP from M. abscessus at OD 0.92 (10.3 mg). (B) 13 C CPMAS spectral overlay of the M. abscessus mAGP and a reconstituted spectrum generated by adding 35% PG, 35% AG, and 30% MA from spectra in . Each spectrum is the result of 40,960 scans.

    Techniques Used: Generated

    (A) CPMAS spectra of untreated M. smegmatis whole cells (OD 0.41, 31.3 mg) and vancomycin (80 μM, 10xMIC) treated M. smegmatis whole cells after 8 h (treated at OD 0.58, harvested at OD 0.53, 50 mg). (B) CPMAS spectra of untreated M. smegmatis (OD 0.94, 57.9 mg) and isoniazid-treated (80 μg/mL, 10xMIC) whole cells after 8 h (treated at OD 0.54, harvested at OD 1.6, 46 mg). Each spectrum is the result of 40,960 scans.
    Figure Legend Snippet: (A) CPMAS spectra of untreated M. smegmatis whole cells (OD 0.41, 31.3 mg) and vancomycin (80 μM, 10xMIC) treated M. smegmatis whole cells after 8 h (treated at OD 0.58, harvested at OD 0.53, 50 mg). (B) CPMAS spectra of untreated M. smegmatis (OD 0.94, 57.9 mg) and isoniazid-treated (80 μg/mL, 10xMIC) whole cells after 8 h (treated at OD 0.54, harvested at OD 1.6, 46 mg). Each spectrum is the result of 40,960 scans.

    Techniques Used:

    m smegmatis strain atcc mc 2  (ATCC)


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    ATCC m smegmatis strain atcc mc 2
    M Smegmatis Strain Atcc Mc 2, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC m smegmatis atcc 700084
    M Smegmatis Atcc 700084, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC m smegmatis atcc 19420
    Live ELISA showing binding activity of MABs GG9 (graphs A and C) and JG7 (graphs B and D) at concentrations 5, 10, and 25 µg/mL to M. tuberculosis (H37Ra; ATCC® 25177™), M. smegmatis (ATCC® 19420™; as a non-pathogenic control), M. avium subsp. avium (ATCC® 15769™), M. bovis (BCG; ATCC® 27290™), M. fortuitum subsp. fortuitum (ATCC® 6841™), and M. intracellulare (ATCC® 13950™) at bacterial dilutions of 4 × 10 5 CFU/mL (neat/undiluted) and 2 × 10 5 CFU/mL (1:2). ELISA, enzyme-linked immunosorbent assay; MABs, monoclonal antibodies; CFU/mL, colony-forming unit per millimeter.
    M Smegmatis Atcc 19420, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC m smegmatis atcc 10231
    Live ELISA showing binding activity of MABs GG9 (graphs A and C) and JG7 (graphs B and D) at concentrations 5, 10, and 25 µg/mL to M. tuberculosis (H37Ra; ATCC® 25177™), M. smegmatis (ATCC® 19420™; as a non-pathogenic control), M. avium subsp. avium (ATCC® 15769™), M. bovis (BCG; ATCC® 27290™), M. fortuitum subsp. fortuitum (ATCC® 6841™), and M. intracellulare (ATCC® 13950™) at bacterial dilutions of 4 × 10 5 CFU/mL (neat/undiluted) and 2 × 10 5 CFU/mL (1:2). ELISA, enzyme-linked immunosorbent assay; MABs, monoclonal antibodies; CFU/mL, colony-forming unit per millimeter.
    M Smegmatis Atcc 10231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC m smegmatis atcc 607
    Live ELISA showing binding activity of MABs GG9 (graphs A and C) and JG7 (graphs B and D) at concentrations 5, 10, and 25 µg/mL to M. tuberculosis (H37Ra; ATCC® 25177™), M. smegmatis (ATCC® 19420™; as a non-pathogenic control), M. avium subsp. avium (ATCC® 15769™), M. bovis (BCG; ATCC® 27290™), M. fortuitum subsp. fortuitum (ATCC® 6841™), and M. intracellulare (ATCC® 13950™) at bacterial dilutions of 4 × 10 5 CFU/mL (neat/undiluted) and 2 × 10 5 CFU/mL (1:2). ELISA, enzyme-linked immunosorbent assay; MABs, monoclonal antibodies; CFU/mL, colony-forming unit per millimeter.
    M Smegmatis Atcc 607, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC m smegmatis atcc
    (A) Mycobacterial cell-wall compositions. (B) 13 C CPMAS spectra of mAGP from M. <t>smegmatis</t> at OD 1.7, OD 3.0, and OD 5.2. Inset shows the growth curve of M. smegmatis in Middlebrook 7H9 Broth with 0.2% glycerol, 10% ADC enrichment and 0.05% Tween-80. (C) 13 C CPMAS spectra comparison of M. smegmatis mAGP from cells harvested at OD 3.0, and the isolated AGP after removal of MA. (D) 13 C CPMAS spectral comparison of M. smegmatis AGP and PG isolated from cells harvested at OD 3.0. Each spectrum is the result of 40,960 scans with sample masses of 22.3 mg mAGP and 7.7 mg PG. (E) 13 C CPMAS spectral comparison of M. smegmatis mAGP harvested at OD 1.0 with spectra acquired as a function of CP time (10.7 mg, 8192 scans each spectrum). (F) Quantitative CP array plot of selected peak intensities of M. smegmatis mAGP spectra obtained as a function of CP time.
    M Smegmatis Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC m smegmatis strain atcc mc 2
    (A) Mycobacterial cell-wall compositions. (B) 13 C CPMAS spectra of mAGP from M. <t>smegmatis</t> at OD 1.7, OD 3.0, and OD 5.2. Inset shows the growth curve of M. smegmatis in Middlebrook 7H9 Broth with 0.2% glycerol, 10% ADC enrichment and 0.05% Tween-80. (C) 13 C CPMAS spectra comparison of M. smegmatis mAGP from cells harvested at OD 3.0, and the isolated AGP after removal of MA. (D) 13 C CPMAS spectral comparison of M. smegmatis AGP and PG isolated from cells harvested at OD 3.0. Each spectrum is the result of 40,960 scans with sample masses of 22.3 mg mAGP and 7.7 mg PG. (E) 13 C CPMAS spectral comparison of M. smegmatis mAGP harvested at OD 1.0 with spectra acquired as a function of CP time (10.7 mg, 8192 scans each spectrum). (F) Quantitative CP array plot of selected peak intensities of M. smegmatis mAGP spectra obtained as a function of CP time.
    M Smegmatis Strain Atcc Mc 2, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Live ELISA showing binding activity of MABs GG9 (graphs A and C) and JG7 (graphs B and D) at concentrations 5, 10, and 25 µg/mL to M. tuberculosis (H37Ra; ATCC® 25177™), M. smegmatis (ATCC® 19420™; as a non-pathogenic control), M. avium subsp. avium (ATCC® 15769™), M. bovis (BCG; ATCC® 27290™), M. fortuitum subsp. fortuitum (ATCC® 6841™), and M. intracellulare (ATCC® 13950™) at bacterial dilutions of 4 × 10 5 CFU/mL (neat/undiluted) and 2 × 10 5 CFU/mL (1:2). ELISA, enzyme-linked immunosorbent assay; MABs, monoclonal antibodies; CFU/mL, colony-forming unit per millimeter.

    Journal: Journal of Clinical Tuberculosis and Other Mycobacterial Diseases

    Article Title: In vitro evaluation of the binding activity of novel mouse IgG1 opsonic monoclonal antibodies to Mycobacterium tuberculosis and other selected mycobacterial species

    doi: 10.1016/j.jctube.2024.100435

    Figure Lengend Snippet: Live ELISA showing binding activity of MABs GG9 (graphs A and C) and JG7 (graphs B and D) at concentrations 5, 10, and 25 µg/mL to M. tuberculosis (H37Ra; ATCC® 25177™), M. smegmatis (ATCC® 19420™; as a non-pathogenic control), M. avium subsp. avium (ATCC® 15769™), M. bovis (BCG; ATCC® 27290™), M. fortuitum subsp. fortuitum (ATCC® 6841™), and M. intracellulare (ATCC® 13950™) at bacterial dilutions of 4 × 10 5 CFU/mL (neat/undiluted) and 2 × 10 5 CFU/mL (1:2). ELISA, enzyme-linked immunosorbent assay; MABs, monoclonal antibodies; CFU/mL, colony-forming unit per millimeter.

    Article Snippet: M. tuberculosis ATCC® 25177™, M. smegmatis ATCC® 19420™, M. bovis ATCC® 27290™, and the clinical M. tuberculosis isolates were cultured in mycobacteria growth indicator tubes (MGIT) by adding 500 µL of the isolate suspension into MGIT tubes containing 7 mL of Middlebrook 7H9 broth supplemented with 800 µL of a reconstituted antibiotic mixture composed of polymyxin B, amphotericin B, nalidixic acid, trimethoprim, and azlocillin (PANTA) and BACTEC MGIT Growth Supplement (Becton Dickinson & Company, USA) consisting of oleic acid, albumin, dextrose, and catalase (OADC).

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Activity Assay

    (A) Mycobacterial cell-wall compositions. (B) 13 C CPMAS spectra of mAGP from M. smegmatis at OD 1.7, OD 3.0, and OD 5.2. Inset shows the growth curve of M. smegmatis in Middlebrook 7H9 Broth with 0.2% glycerol, 10% ADC enrichment and 0.05% Tween-80. (C) 13 C CPMAS spectra comparison of M. smegmatis mAGP from cells harvested at OD 3.0, and the isolated AGP after removal of MA. (D) 13 C CPMAS spectral comparison of M. smegmatis AGP and PG isolated from cells harvested at OD 3.0. Each spectrum is the result of 40,960 scans with sample masses of 22.3 mg mAGP and 7.7 mg PG. (E) 13 C CPMAS spectral comparison of M. smegmatis mAGP harvested at OD 1.0 with spectra acquired as a function of CP time (10.7 mg, 8192 scans each spectrum). (F) Quantitative CP array plot of selected peak intensities of M. smegmatis mAGP spectra obtained as a function of CP time.

    Journal: Journal of magnetic resonance open

    Article Title: CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells

    doi: 10.1016/j.jmro.2023.100127

    Figure Lengend Snippet: (A) Mycobacterial cell-wall compositions. (B) 13 C CPMAS spectra of mAGP from M. smegmatis at OD 1.7, OD 3.0, and OD 5.2. Inset shows the growth curve of M. smegmatis in Middlebrook 7H9 Broth with 0.2% glycerol, 10% ADC enrichment and 0.05% Tween-80. (C) 13 C CPMAS spectra comparison of M. smegmatis mAGP from cells harvested at OD 3.0, and the isolated AGP after removal of MA. (D) 13 C CPMAS spectral comparison of M. smegmatis AGP and PG isolated from cells harvested at OD 3.0. Each spectrum is the result of 40,960 scans with sample masses of 22.3 mg mAGP and 7.7 mg PG. (E) 13 C CPMAS spectral comparison of M. smegmatis mAGP harvested at OD 1.0 with spectra acquired as a function of CP time (10.7 mg, 8192 scans each spectrum). (F) Quantitative CP array plot of selected peak intensities of M. smegmatis mAGP spectra obtained as a function of CP time.

    Article Snippet: M. smegmatis ATCC 700,084 and M. abscessus ATCC 19,977 were grown in Middlebrook 7H9 Broth with 0.2% glycerol, 10% ADC (albumin-dextrose-catalase) enrichment and 0.05% Tween-80.

    Techniques: Comparison, Isolation

    (A) 13 C CPMAS spectra of pure MA from M. tuberculosis (15.3 mg), AG from Larchwood (28.2 mg), and PG isolated from M. smegmatis (7.7 mg). (B) 13 C CPMAS spectrum of M. smegmatis mAGP from cells harvested at OD 1.0, and a reconstituted spectrum generated by adding 50% PG, 20% AG, and 30% AG from spectra in . Each spectrum is the result of 40,960 scans.

    Journal: Journal of magnetic resonance open

    Article Title: CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells

    doi: 10.1016/j.jmro.2023.100127

    Figure Lengend Snippet: (A) 13 C CPMAS spectra of pure MA from M. tuberculosis (15.3 mg), AG from Larchwood (28.2 mg), and PG isolated from M. smegmatis (7.7 mg). (B) 13 C CPMAS spectrum of M. smegmatis mAGP from cells harvested at OD 1.0, and a reconstituted spectrum generated by adding 50% PG, 20% AG, and 30% AG from spectra in . Each spectrum is the result of 40,960 scans.

    Article Snippet: M. smegmatis ATCC 700,084 and M. abscessus ATCC 19,977 were grown in Middlebrook 7H9 Broth with 0.2% glycerol, 10% ADC (albumin-dextrose-catalase) enrichment and 0.05% Tween-80.

    Techniques: Isolation, Generated

    (A) 13 C CPMAS spectra of mAGP from M. smegmatis at OD 1.0 (24.5 mg), and mAGP from M. abscessus at OD 0.92 (10.3 mg). (B) 13 C CPMAS spectral overlay of the M. abscessus mAGP and a reconstituted spectrum generated by adding 35% PG, 35% AG, and 30% MA from spectra in . Each spectrum is the result of 40,960 scans.

    Journal: Journal of magnetic resonance open

    Article Title: CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells

    doi: 10.1016/j.jmro.2023.100127

    Figure Lengend Snippet: (A) 13 C CPMAS spectra of mAGP from M. smegmatis at OD 1.0 (24.5 mg), and mAGP from M. abscessus at OD 0.92 (10.3 mg). (B) 13 C CPMAS spectral overlay of the M. abscessus mAGP and a reconstituted spectrum generated by adding 35% PG, 35% AG, and 30% MA from spectra in . Each spectrum is the result of 40,960 scans.

    Article Snippet: M. smegmatis ATCC 700,084 and M. abscessus ATCC 19,977 were grown in Middlebrook 7H9 Broth with 0.2% glycerol, 10% ADC (albumin-dextrose-catalase) enrichment and 0.05% Tween-80.

    Techniques: Generated

    (A) CPMAS spectra of untreated M. smegmatis whole cells (OD 0.41, 31.3 mg) and vancomycin (80 μM, 10xMIC) treated M. smegmatis whole cells after 8 h (treated at OD 0.58, harvested at OD 0.53, 50 mg). (B) CPMAS spectra of untreated M. smegmatis (OD 0.94, 57.9 mg) and isoniazid-treated (80 μg/mL, 10xMIC) whole cells after 8 h (treated at OD 0.54, harvested at OD 1.6, 46 mg). Each spectrum is the result of 40,960 scans.

    Journal: Journal of magnetic resonance open

    Article Title: CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells

    doi: 10.1016/j.jmro.2023.100127

    Figure Lengend Snippet: (A) CPMAS spectra of untreated M. smegmatis whole cells (OD 0.41, 31.3 mg) and vancomycin (80 μM, 10xMIC) treated M. smegmatis whole cells after 8 h (treated at OD 0.58, harvested at OD 0.53, 50 mg). (B) CPMAS spectra of untreated M. smegmatis (OD 0.94, 57.9 mg) and isoniazid-treated (80 μg/mL, 10xMIC) whole cells after 8 h (treated at OD 0.54, harvested at OD 1.6, 46 mg). Each spectrum is the result of 40,960 scans.

    Article Snippet: M. smegmatis ATCC 700,084 and M. abscessus ATCC 19,977 were grown in Middlebrook 7H9 Broth with 0.2% glycerol, 10% ADC (albumin-dextrose-catalase) enrichment and 0.05% Tween-80.

    Techniques: