m pneumoniae m129  (ATCC)


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    Name:
    Mycoplasma pneumoniae M129 B7
    Description:

    Catalog Number:
    29342
    Price:
    None
    Applications:
    Respiratory research
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    Structured Review

    ATCC m pneumoniae m129
    Crystal violet stain of HeLa cells after mycoplasmal infection. Cytotoxicity of M. <t>pneumoniae</t> strains toward HeLa cell cultures. Confluent HeLa cell culture without infection or with <t>M129</t> wild type cells and with GPM113 ( mpn400 :Tn) mutant cells. (A) After 20 h, 48 h, and 96 h post infection HeLa cells were stained with crystal violet and photographed. (B) OD 595 measurements were plotted and statistical significance of the cytotoxicities in different strains at the two time points was calculated. For the original data, see Supplementary Data Sheet S1 .

    https://www.bioz.com/result/m pneumoniae m129/product/ATCC
    Average 96 stars, based on 7 article reviews
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    Images

    1) Product Images from "Characterization of an Immunoglobulin Binding Protein (IbpM) From Mycoplasma pneumoniae"

    Article Title: Characterization of an Immunoglobulin Binding Protein (IbpM) From Mycoplasma pneumoniae

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2020.00685

    Crystal violet stain of HeLa cells after mycoplasmal infection. Cytotoxicity of M. pneumoniae strains toward HeLa cell cultures. Confluent HeLa cell culture without infection or with M129 wild type cells and with GPM113 ( mpn400 :Tn) mutant cells. (A) After 20 h, 48 h, and 96 h post infection HeLa cells were stained with crystal violet and photographed. (B) OD 595 measurements were plotted and statistical significance of the cytotoxicities in different strains at the two time points was calculated. For the original data, see Supplementary Data Sheet S1 .
    Figure Legend Snippet: Crystal violet stain of HeLa cells after mycoplasmal infection. Cytotoxicity of M. pneumoniae strains toward HeLa cell cultures. Confluent HeLa cell culture without infection or with M129 wild type cells and with GPM113 ( mpn400 :Tn) mutant cells. (A) After 20 h, 48 h, and 96 h post infection HeLa cells were stained with crystal violet and photographed. (B) OD 595 measurements were plotted and statistical significance of the cytotoxicities in different strains at the two time points was calculated. For the original data, see Supplementary Data Sheet S1 .

    Techniques Used: Staining, Infection, Cell Culture, Mutagenesis

    Related Articles

    Diagnostic Assay:

    Article Title: Development of a Multilocus Sequence Typing Scheme for Molecular Typing of Mycoplasma pneumoniae
    Article Snippet: .. Fifty-five M. pneumoniae strains were submitted to Public Health England, United Kingdom, for clinical diagnostic purposes, and the two M. pneumoniae type strains, FH (NCTC 10119, ATCC 15531) and M129 (ATCC 29342), were obtained from National Collection of Type Cultures (NCTC) (held by Public Health England). .. All strains were triple cloned on Mycoplasma agar (Mycoplasma Experience, Surrey, United Kingdom) and confirmed to be M. pneumonia e by amplification of the p1 gene ( ).

    Clone Assay:

    Article Title: Functional Analysis of the Superfamily 1 DNA Helicases Encoded by Mycoplasma pneumoniae and Mycoplasma genitalium
    Article Snippet: .. Cloning of the MPN340, MPNE_0394, MPN341 and MG244 ORFs Bacterial genomic DNA was purified from cultures of M. genitalium strain G37 (ATCC® no. 33530™) and M. pneumoniae strains M129 (ATCC® no. 29342™) and FH (ATCC® no. 15531™), using previously described procedures . .. Before cloning of ORFs MPN340 and MPNE_0394 from M. pneumoniae strains M129 and FH, respectively, a TGA codon within these ORFs was changed into a TGG codon using a PCR-based mutagenesis method .

    Incubation:

    Article Title: In vitro subminimum inhibitory concentrations of macrolide antibiotics induce macrolide resistance in Mycoplasma pneumoniae
    Article Snippet: .. One M. pneumoniae reference strain M129 (ATCC 29342) and 104 clinical isolates (200 μL for each sample) were inoculated in 2 mL medium in a 10 mL culture tube and incubated at 37o C for 6-8 days. ..

    other:

    Article Title: A sensitive and rapid immunoassay for Mycoplasma pneumoniae in children with pneumonia based on single-walled carbon nanotubes
    Article Snippet: Single-walled nanotubes were purchased from Beijing DK Daoking Technology Co.Standard M. pneumoniae FH (ATCC 15531) and M129 (ATCC 29342) strains were purchased from ATCC.

    Purification:

    Article Title: Functional Analysis of the Superfamily 1 DNA Helicases Encoded by Mycoplasma pneumoniae and Mycoplasma genitalium
    Article Snippet: .. Cloning of the MPN340, MPNE_0394, MPN341 and MG244 ORFs Bacterial genomic DNA was purified from cultures of M. genitalium strain G37 (ATCC® no. 33530™) and M. pneumoniae strains M129 (ATCC® no. 29342™) and FH (ATCC® no. 15531™), using previously described procedures . .. Before cloning of ORFs MPN340 and MPNE_0394 from M. pneumoniae strains M129 and FH, respectively, a TGA codon within these ORFs was changed into a TGG codon using a PCR-based mutagenesis method .

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    ATCC m pneumoniae reference strain m129
    A) Polymerase chain reaction (PCR) amplification of ribosomal protein L4 gene from M. <t>pneumoniae</t> clinical iso- lates. 1: DNA marker; 2: M. pneumoniae reference strain <t>M129;</t> 3-11: PCR products (464 bp) of ribosomal protein L4 gene; 12: Negative control.
    M Pneumoniae Reference Strain M129, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC m pneumoniae strain m129
    Analytical sensitivity of M. <t>pneumoniae</t> detection by ELITe InGenius and repMp1 PCR assays. Ten-fold serial culture dilutions and subsequent detection of M. pneumoniae <t>M129</t> (ATCC 29343) showing cycle threshold ( C T ) versus CFU per milliliter for ELITe InGenius PCR (A) and repMp1 PCR (B). Ten-fold serial DNA dilutions and subsequent detection of M. pneumoniae M129 (ATCC 29343) showing C T versus CFU per milliliter for ELITe InGenius PCR (C) and repMp1 PCR (D). Graphs depict group mean ± standard deviation. Each assay was run twice to ensure reproducibility with specimens tested in duplicate within each run. The ELITech PCR LoD was 0.16 CFU/PCR test or 4.16 genome copies (GC)/test. The repMp1 assay LoD was 0.41 CFU/PCR test or 1.66 GC/test. R -squared values for each dilution curve showed significant association between CFU per milliliter concentrations and C T values (0.995 for ELITe InGenius PCR and 0.965 for repMp1 PCR).
    M Pneumoniae Strain M129, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m pneumoniae strain m129/product/ATCC
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    m pneumoniae strain m129 - by Bioz Stars, 2020-07
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    Image Search Results


    A) Polymerase chain reaction (PCR) amplification of ribosomal protein L4 gene from M. pneumoniae clinical iso- lates. 1: DNA marker; 2: M. pneumoniae reference strain M129; 3-11: PCR products (464 bp) of ribosomal protein L4 gene; 12: Negative control.

    Journal: Hippokratia

    Article Title: In vitro subminimum inhibitory concentrations of macrolide antibiotics induce macrolide resistance in Mycoplasma pneumoniae

    doi:

    Figure Lengend Snippet: A) Polymerase chain reaction (PCR) amplification of ribosomal protein L4 gene from M. pneumoniae clinical iso- lates. 1: DNA marker; 2: M. pneumoniae reference strain M129; 3-11: PCR products (464 bp) of ribosomal protein L4 gene; 12: Negative control.

    Article Snippet: One M. pneumoniae reference strain M129 (ATCC 29342) and 104 clinical isolates (200 μL for each sample) were inoculated in 2 mL medium in a 10 mL culture tube and incubated at 37o C for 6-8 days.

    Techniques: Polymerase Chain Reaction, Amplification, Marker, Negative Control

    A) Polymerase chain reaction (PCR) amplification of 23SrRNA gene including 2063 and 2064 from M. pneumoniae clinical isolates. 1: DNA marker; 2: M. pneumoniae reference strain M129; 3-11: PCR products (303 bp) of 23SrRNA gene (including 2063 and 2064).

    Journal: Hippokratia

    Article Title: In vitro subminimum inhibitory concentrations of macrolide antibiotics induce macrolide resistance in Mycoplasma pneumoniae

    doi:

    Figure Lengend Snippet: A) Polymerase chain reaction (PCR) amplification of 23SrRNA gene including 2063 and 2064 from M. pneumoniae clinical isolates. 1: DNA marker; 2: M. pneumoniae reference strain M129; 3-11: PCR products (303 bp) of 23SrRNA gene (including 2063 and 2064).

    Article Snippet: One M. pneumoniae reference strain M129 (ATCC 29342) and 104 clinical isolates (200 μL for each sample) were inoculated in 2 mL medium in a 10 mL culture tube and incubated at 37o C for 6-8 days.

    Techniques: Polymerase Chain Reaction, Amplification, Marker

    Predicted domain structure and purification of the PcrA-like proteins from M. pneumoniae and M. genitalium . (A) Schematic representation of the predicted (sub)domains (1A, 1B, 2A and 2B) and motifs (I, Ia and II to VI) of the PcrA-like proteins from M. pneumoniae and M. genitalium . The predictions are based on the multiple alignment shown in supporting Fig. S1 , and on the crystal structures of PcrA/UvrD/Rep homologs [35] , [60] . Each subdomain is indicated by a separate color. Counterparts of the predicted 2B domains from PcrA Mpn and PcrA Mge (in blue) are absent from the PcrA s M129 and PcrA s FH proteins. (B) ORF structure of the genomes of M. pneumoniae ( Mpn , at the top) and M. genitalium ( Mge , at the bottom) in the region surrounding the ORFs encoding the PcrA-like proteins. All ORFs in these regions have the same orientation, i.e. 5′→3′ from left to right. The ORFs encoding the PcrA-like proteins are presented with the same color scheme as that of their encoded proteins in (A). Neighboring ORFs that are conserved between M. pneumoniae and M. genitalium are indicated in dark grey. The cluster of genes that is unique to M. pneumoniae (consisting of ORFs MPN342 to MPN347) is indicated at the top. (C) Purification of MBP, PcrA s M129 , K29A, K29R, PcrA s FH , PcrA Mpn and PcrA Mge . K29A and K29R are point mutants of protein PcrA s M129. All proteins were purified as fusions to MBP by using the same protocol. Some of the protein preparations, including those of MBP and PcrA Mge , contained minor protein species that are smaller than the full-length proteins. These species are not contaminants, but breakdown products of the full-length proteins; this was demonstrated by MALDI-TOF analysis of the two protein species that are indicated with asterisks (*). Samples of the purified proteins (as indicated above the lanes) were analyzed by SDS-PAGE (10%) and Coomassie brilliant blue (CBB)-staining. The sizes of protein markers (lane 1; PageRulerTM Prestained Protein Ladder [Fermentas]) are shown on the left-hand side of the figure in kDa.

    Journal: PLoS ONE

    Article Title: Functional Analysis of the Superfamily 1 DNA Helicases Encoded by Mycoplasma pneumoniae and Mycoplasma genitalium

    doi: 10.1371/journal.pone.0070870

    Figure Lengend Snippet: Predicted domain structure and purification of the PcrA-like proteins from M. pneumoniae and M. genitalium . (A) Schematic representation of the predicted (sub)domains (1A, 1B, 2A and 2B) and motifs (I, Ia and II to VI) of the PcrA-like proteins from M. pneumoniae and M. genitalium . The predictions are based on the multiple alignment shown in supporting Fig. S1 , and on the crystal structures of PcrA/UvrD/Rep homologs [35] , [60] . Each subdomain is indicated by a separate color. Counterparts of the predicted 2B domains from PcrA Mpn and PcrA Mge (in blue) are absent from the PcrA s M129 and PcrA s FH proteins. (B) ORF structure of the genomes of M. pneumoniae ( Mpn , at the top) and M. genitalium ( Mge , at the bottom) in the region surrounding the ORFs encoding the PcrA-like proteins. All ORFs in these regions have the same orientation, i.e. 5′→3′ from left to right. The ORFs encoding the PcrA-like proteins are presented with the same color scheme as that of their encoded proteins in (A). Neighboring ORFs that are conserved between M. pneumoniae and M. genitalium are indicated in dark grey. The cluster of genes that is unique to M. pneumoniae (consisting of ORFs MPN342 to MPN347) is indicated at the top. (C) Purification of MBP, PcrA s M129 , K29A, K29R, PcrA s FH , PcrA Mpn and PcrA Mge . K29A and K29R are point mutants of protein PcrA s M129. All proteins were purified as fusions to MBP by using the same protocol. Some of the protein preparations, including those of MBP and PcrA Mge , contained minor protein species that are smaller than the full-length proteins. These species are not contaminants, but breakdown products of the full-length proteins; this was demonstrated by MALDI-TOF analysis of the two protein species that are indicated with asterisks (*). Samples of the purified proteins (as indicated above the lanes) were analyzed by SDS-PAGE (10%) and Coomassie brilliant blue (CBB)-staining. The sizes of protein markers (lane 1; PageRulerTM Prestained Protein Ladder [Fermentas]) are shown on the left-hand side of the figure in kDa.

    Article Snippet: Cloning of the MPN340, MPNE_0394, MPN341 and MG244 ORFs Bacterial genomic DNA was purified from cultures of M. genitalium strain G37 (ATCC® no. 33530™) and M. pneumoniae strains M129 (ATCC® no. 29342™) and FH (ATCC® no. 15531™), using previously described procedures .

    Techniques: Purification, IA, SDS Page, Staining

    Adherence of M. pneumoniae cells to HBEC after pretreatment of mycoplasmas with preimmune serum, anti-RP14 serum, anti-RP30 serum, anti-HP14/30 serum, and serum from an animal immunized subcutaneously with M. pneumoniae M129. In each experimental run, all samples were tested as duplicates or triplicates. Values represent the arithmetic means ± standard deviations of at least two independent experiments and are expressed as a percentage of the control (preimmune serum). The broken line represents the maximal level of attachment of M. pneumoniae cells when cells were preincubated with preimmune serum.

    Journal: Infection and Immunity

    Article Title: Strategy To Create Chimeric Proteins Derived from Functional Adhesin Regions of Mycoplasma pneumoniae for Vaccine Development ▿ for Vaccine Development ▿ †

    doi: 10.1128/IAI.00268-09

    Figure Lengend Snippet: Adherence of M. pneumoniae cells to HBEC after pretreatment of mycoplasmas with preimmune serum, anti-RP14 serum, anti-RP30 serum, anti-HP14/30 serum, and serum from an animal immunized subcutaneously with M. pneumoniae M129. In each experimental run, all samples were tested as duplicates or triplicates. Values represent the arithmetic means ± standard deviations of at least two independent experiments and are expressed as a percentage of the control (preimmune serum). The broken line represents the maximal level of attachment of M. pneumoniae cells when cells were preincubated with preimmune serum.

    Article Snippet: The M. pneumoniae wild-type strain M129 (ATCC 29342) was grown in 50 ml of PPLO medium (Difco, Becton Dickinson, Sparks, MD) in 150-cm2 tissue culture flasks (Greiner, Frickenhausen, Germany) at 37°C ( ).

    Techniques:

    (A) Results of immunoblot analysis of recombinant proteins from the P1 adhesin (RP1 to RP15) and the protein P30 (RP30) of M. pneumoniae with 14 human sera that tested positive for IgA and IgM antibodies to M. pneumoniae by commercial ELISA. (B) ELISAs of recombinant proteins RP1 to RP15 and RP30 with hyperimmune serum from guinea pigs immunized subcutaneously (s.c.) or stimulated intranasally (i.n.) with M. pneumoniae M129 (arithmetic mean of OD 450/620 values of four replicates with standard deviations). (C) Adhesion inhibition assay. Cytadherence of M. pneumoniae M129 to HBEC (HBEpC), MRC-5 cells, and HeLa cells after preincubation of mycoplasmas with monospecific antiserum against recombinant proteins RP1 to RP15 and RP30. Preimmune serum (NS; tested after 0 and 1 h of incubation of opsonized mycoplasmas with human cells) and anti-RPADK serum served as negative controls. Hyperimmune serum (PS) from an animal immunized subcutaneously with M. pneumoniae M129 served as a positive control. In each experimental run, all samples were tested as duplicates or triplicates, and tests were repeated on at least three different experimental days. Values represent the arithmetic means with standard deviations and are expressed as a percentage of the control (anti-RPADK). Significant levels for reduction of cytadherence in comparison to the effect of anti-RPADK were set at a P value of

    Journal: Infection and Immunity

    Article Title: Strategy To Create Chimeric Proteins Derived from Functional Adhesin Regions of Mycoplasma pneumoniae for Vaccine Development ▿ for Vaccine Development ▿ †

    doi: 10.1128/IAI.00268-09

    Figure Lengend Snippet: (A) Results of immunoblot analysis of recombinant proteins from the P1 adhesin (RP1 to RP15) and the protein P30 (RP30) of M. pneumoniae with 14 human sera that tested positive for IgA and IgM antibodies to M. pneumoniae by commercial ELISA. (B) ELISAs of recombinant proteins RP1 to RP15 and RP30 with hyperimmune serum from guinea pigs immunized subcutaneously (s.c.) or stimulated intranasally (i.n.) with M. pneumoniae M129 (arithmetic mean of OD 450/620 values of four replicates with standard deviations). (C) Adhesion inhibition assay. Cytadherence of M. pneumoniae M129 to HBEC (HBEpC), MRC-5 cells, and HeLa cells after preincubation of mycoplasmas with monospecific antiserum against recombinant proteins RP1 to RP15 and RP30. Preimmune serum (NS; tested after 0 and 1 h of incubation of opsonized mycoplasmas with human cells) and anti-RPADK serum served as negative controls. Hyperimmune serum (PS) from an animal immunized subcutaneously with M. pneumoniae M129 served as a positive control. In each experimental run, all samples were tested as duplicates or triplicates, and tests were repeated on at least three different experimental days. Values represent the arithmetic means with standard deviations and are expressed as a percentage of the control (anti-RPADK). Significant levels for reduction of cytadherence in comparison to the effect of anti-RPADK were set at a P value of

    Article Snippet: The M. pneumoniae wild-type strain M129 (ATCC 29342) was grown in 50 ml of PPLO medium (Difco, Becton Dickinson, Sparks, MD) in 150-cm2 tissue culture flasks (Greiner, Frickenhausen, Germany) at 37°C ( ).

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Inhibition, Incubation, Positive Control

    ) and P30 (MPN453) of M. pneumoniae strain M129 and location of recombinant proteins RP1 to RP15 and RP30.

    Journal: Infection and Immunity

    Article Title: Strategy To Create Chimeric Proteins Derived from Functional Adhesin Regions of Mycoplasma pneumoniae for Vaccine Development ▿ for Vaccine Development ▿ †

    doi: 10.1128/IAI.00268-09

    Figure Lengend Snippet: ) and P30 (MPN453) of M. pneumoniae strain M129 and location of recombinant proteins RP1 to RP15 and RP30.

    Article Snippet: The M. pneumoniae wild-type strain M129 (ATCC 29342) was grown in 50 ml of PPLO medium (Difco, Becton Dickinson, Sparks, MD) in 150-cm2 tissue culture flasks (Greiner, Frickenhausen, Germany) at 37°C ( ).

    Techniques: Recombinant

    Analysis of chimeric protein HP14/30 and the monospecific antiserum derived. (A) SDS-PAGE of purified protein HP14/30 (lane 1) stained with Coomassie blue. (B) Reaction of chimeric protein HP14/30 with anti-His monoclonal antibody (lane 1), polyclonal anti- M. pneumoniae M129 (lane 2), and anti-RP14 (lane 3) and anti-RP30 (lane 4) guinea pig serum. (C) Reaction of M. pneumoniae M129 whole-cell antigen with polyclonal guinea pig antiserum against the recombinant proteins HP14/30 (lane 1), RP14 (lane 2), and RP30 (lane 3). Lanes M, molecular mass markers.

    Journal: Infection and Immunity

    Article Title: Strategy To Create Chimeric Proteins Derived from Functional Adhesin Regions of Mycoplasma pneumoniae for Vaccine Development ▿ for Vaccine Development ▿ †

    doi: 10.1128/IAI.00268-09

    Figure Lengend Snippet: Analysis of chimeric protein HP14/30 and the monospecific antiserum derived. (A) SDS-PAGE of purified protein HP14/30 (lane 1) stained with Coomassie blue. (B) Reaction of chimeric protein HP14/30 with anti-His monoclonal antibody (lane 1), polyclonal anti- M. pneumoniae M129 (lane 2), and anti-RP14 (lane 3) and anti-RP30 (lane 4) guinea pig serum. (C) Reaction of M. pneumoniae M129 whole-cell antigen with polyclonal guinea pig antiserum against the recombinant proteins HP14/30 (lane 1), RP14 (lane 2), and RP30 (lane 3). Lanes M, molecular mass markers.

    Article Snippet: The M. pneumoniae wild-type strain M129 (ATCC 29342) was grown in 50 ml of PPLO medium (Difco, Becton Dickinson, Sparks, MD) in 150-cm2 tissue culture flasks (Greiner, Frickenhausen, Germany) at 37°C ( ).

    Techniques: Derivative Assay, SDS Page, Purification, Staining, Recombinant

    Analytical sensitivity of M. pneumoniae detection by ELITe InGenius and repMp1 PCR assays. Ten-fold serial culture dilutions and subsequent detection of M. pneumoniae M129 (ATCC 29343) showing cycle threshold ( C T ) versus CFU per milliliter for ELITe InGenius PCR (A) and repMp1 PCR (B). Ten-fold serial DNA dilutions and subsequent detection of M. pneumoniae M129 (ATCC 29343) showing C T versus CFU per milliliter for ELITe InGenius PCR (C) and repMp1 PCR (D). Graphs depict group mean ± standard deviation. Each assay was run twice to ensure reproducibility with specimens tested in duplicate within each run. The ELITech PCR LoD was 0.16 CFU/PCR test or 4.16 genome copies (GC)/test. The repMp1 assay LoD was 0.41 CFU/PCR test or 1.66 GC/test. R -squared values for each dilution curve showed significant association between CFU per milliliter concentrations and C T values (0.995 for ELITe InGenius PCR and 0.965 for repMp1 PCR).

    Journal: Journal of Clinical Microbiology

    Article Title: Evaluation of the ELITe InGenius PCR Platform for Detection of Mycoplasma pneumoniae

    doi: 10.1128/JCM.00287-19

    Figure Lengend Snippet: Analytical sensitivity of M. pneumoniae detection by ELITe InGenius and repMp1 PCR assays. Ten-fold serial culture dilutions and subsequent detection of M. pneumoniae M129 (ATCC 29343) showing cycle threshold ( C T ) versus CFU per milliliter for ELITe InGenius PCR (A) and repMp1 PCR (B). Ten-fold serial DNA dilutions and subsequent detection of M. pneumoniae M129 (ATCC 29343) showing C T versus CFU per milliliter for ELITe InGenius PCR (C) and repMp1 PCR (D). Graphs depict group mean ± standard deviation. Each assay was run twice to ensure reproducibility with specimens tested in duplicate within each run. The ELITech PCR LoD was 0.16 CFU/PCR test or 4.16 genome copies (GC)/test. The repMp1 assay LoD was 0.41 CFU/PCR test or 1.66 GC/test. R -squared values for each dilution curve showed significant association between CFU per milliliter concentrations and C T values (0.995 for ELITe InGenius PCR and 0.965 for repMp1 PCR).

    Article Snippet: Frozen aliquots of M. pneumoniae strain M129 (ATCC 29343) of known CFU per milliliter were thawed and serially diluted in 10-fold increments in SP4 broth to provide a broad range of concentrations (107 CFU/ml to 10−2 CFU/ml).

    Techniques: Polymerase Chain Reaction, Standard Deviation